SRR3548448_1.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR3548448_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	254856
Sequences flagged as poor quality	0
Sequence length	51
%GC	49

Per base sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
AATGATACCTGTCTCTTATACACATCTGACGCTGAAGCCATCGTATGCCGT	2589	1.015867784160467	No Hit
AATGATACGGCTGTCTCTTATACACATCTGACGCTGAAGCCATCGTATGCC	2485	0.9750604262799384	No Hit
CTGTCTCTTATACACATCTGACGCTGAAGCCATCGTATGCCGTCTTCTGCT	2291	0.8989390086951062	No Hit
AATCTGTCTCTTATACACATCTGACGCTGAAGCCATCGTATGCCGTCTTCT	1975	0.7749474212888847	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1503	0.5897447970618702	No Hit
AAACTGTCTCTTATACACATCTGACGCTGAAGCCATCGTATGCCGTCTTCT	576	0.22600998210754308	No Hit
AATGACTGTCTCTTATACACATCTGACGCTGAAGCCATCGTATGCCGTCTT	561	0.22012430549015913	No Hit
TGCAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGG	492	0.19305019305019305	No Hit
AATGATCTGTCTCTTATACACATCTGACGCTGAAGCCATCGTATGCCGTCT	481	0.18873403019744484	No Hit
AATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCTGAAGCCATC	443	0.17382364943340553	No Hit
TCCCCCGGGAATGATACCTGTCTCTTATACACATCTGACGCTGAAGCCATC	413	0.16205229619863765	No Hit
AACTGTCTCTTATACACATCTGACGCTGAAGCCATCGTATGCCGTCTTCTG	376	0.14753429387575728	No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	367	0.14400288790532692	No Hit
AAAAACTGTCTCTTATACACATCTGACGCTGAAGCCATCGTATGCCGTCTT	362	0.1420409956995323	No Hit
AATGATACGGCGACTGTCTCTTATACACATCTGACGCTGAAGCCATCGTAT	359	0.1408638603760555	No Hit
CGCAACGGGAGAAACAAGCGAGATAGGAATGTCTTACACGCGGGGCAAGAC	279	0.10947358508334118	No Hit
ATAGAAGGGATCTCAGCATTTTGATAATTTAGGCTTACCATAGAAGTTACT	279	0.10947358508334118	No Hit
CCTGTCTCTTATACACATCTGACGCTGAAGCCATCGTATGCCGTCTTCTGC	274	0.10751169287754653	No Hit
TCCCCCGGGAATGATACGGCTGTCTCTTATACACATCTGACGCTGAAGCCA	261	0.10241077314248047	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	260	0.10201839470132153	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACTATGG	70	0.0	45.000004	2
ACAGTCG	25	3.8822353E-5	45.000004	1
GGTACAA	25	3.8822353E-5	45.000004	8
CAAACGG	40	6.7793735E-9	45.000004	2
CGAACAG	20	7.022944E-4	45.000004	1
ACTGCGC	20	7.022944E-4	45.000004	38
GCGACTC	20	7.022944E-4	45.000004	43
GCGACGT	25	3.8822353E-5	45.000004	37
CTGTATA	20	7.022944E-4	45.000004	17
ATAGGTA	25	3.8822353E-5	45.000004	23
GTCGACA	20	7.022944E-4	45.000004	42
CGAGTCT	20	7.022944E-4	45.000004	16
CGACGTC	25	3.8822353E-5	45.000004	38
CGAGTAC	40	6.7793735E-9	45.000004	27
CATGCGG	35	1.206954E-7	45.000004	2
CCCTATC	25	3.8822353E-5	45.000004	29
TTAGCAC	20	7.022944E-4	45.000004	14
TCACACG	20	7.022944E-4	45.000004	1
TTTCGTC	25	3.8822353E-5	45.000004	16
GCAACGG	80	0.0	45.000004	2

Summary

Basic Statistics

Per base sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content