FastQCFastQC Report
Sat 18 Jun 2016
SRR3548447_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3548447_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences260372
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AATGATACGGCTGTCTCTTATACACATCTGACGCTGAAGCCATCGTATGCC29561.1352987264375587No Hit
AATGATACCTGTCTCTTATACACATCTGACGCTGAAGCCATCGTATGCCGT29281.12454488193815No Hit
CTGTCTCTTATACACATCTGACGCTGAAGCCATCGTATGCCGTCTTCTGCT25850.9928102868203954No Hit
AATCTGTCTCTTATACACATCTGACGCTGAAGCCATCGTATGCCGTCTTCT22880.8787427219516691No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA14910.5726422195935047No Hit
AATGACTGTCTCTTATACACATCTGACGCTGAAGCCATCGTATGCCGTCTT6850.26308512436053033No Hit
AAACTGTCTCTTATACACATCTGACGCTGAAGCCATCGTATGCCGTCTTCT5890.2262148003625582No Hit
AATGATCTGTCTCTTATACACATCTGACGCTGAAGCCATCGTATGCCGTCT5590.21469282411319188No Hit
TGCAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGG5240.20125051848893122No Hit
AATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCTGAAGCCATC4940.18972854223956492No Hit
TCCCCCGGGAATGATACCTGTCTCTTATACACATCTGACGCTGAAGCCATC4570.17551810486534652No Hit
AACTGTCTCTTATACACATCTGACGCTGAAGCCATCGTATGCCGTCTTCTG4500.17282964374049437No Hit
AATGATACGGCGACTGTCTCTTATACACATCTGACGCTGAAGCCATCGTAT4220.16207579924108584No Hit
AAAAACTGTCTCTTATACACATCTGACGCTGAAGCCATCGTATGCCGTCTT4030.15477854761648718No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3680.1413362419922265No Hit
CCTGTCTCTTATACACATCTGACGCTGAAGCCATCGTATGCCGTCTTCTGC3290.12635767286805033No Hit
TCCCCCGGGAATGATACGGCTGTCTCTTATACACATCTGACGCTGAAGCCA2960.1136834989937474No Hit
CGCAACGGGAGAAACAAGCGAGATAGGAATGTCTTACACGCGGGGCAAGAC2680.10292965449433887No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2620.10062525924446561No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CATGCGG453.8198777E-1045.0000042
TACCTAG650.045.0000041
TCAAACG453.8198777E-1045.0000041
TATCATG253.882468E-545.01
CTTAACG207.0232264E-445.01
TGTCATA207.0232264E-445.045
CAGGTAG207.0232264E-445.020
ACTATAG253.882468E-545.01
ATCTCAA207.0232264E-445.018
GGTACAA253.882468E-545.08
CAAACGG551.8189894E-1245.02
CGAACCG207.0232264E-445.01
GCGACTC253.882468E-545.043
CTACTGA207.0232264E-445.013
CGTATAG207.0232264E-445.01
CTGTAAG207.0232264E-445.014
CGAAATA207.0232264E-445.011
CGAGTGC207.0232264E-445.031
CGACGTT207.0232264E-445.024
ACGCATG207.0232264E-445.01
Produced by FastQC (version 0.11.5)