##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3548447_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	260372
Sequences flagged as poor quality	0
Sequence length	51
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.744046978937828	31.0	31.0	34.0	30.0	34.0
2	31.949195766057795	33.0	31.0	34.0	30.0	34.0
3	32.072676785522255	34.0	31.0	34.0	30.0	34.0
4	35.72836556926244	37.0	35.0	37.0	33.0	37.0
5	35.51673375017283	37.0	35.0	37.0	33.0	37.0
6	35.53061773155331	37.0	35.0	37.0	33.0	37.0
7	35.50697079563086	37.0	35.0	37.0	33.0	37.0
8	35.57483907639838	37.0	35.0	37.0	35.0	37.0
9	37.23583949118953	39.0	37.0	39.0	34.0	39.0
10	36.89379426359209	39.0	37.0	39.0	33.0	39.0
11	36.90405650377153	39.0	37.0	39.0	33.0	39.0
12	36.774503402823655	39.0	35.0	39.0	33.0	39.0
13	36.78847187869663	39.0	35.0	39.0	33.0	39.0
14	37.86744734456854	40.0	37.0	41.0	33.0	41.0
15	37.933944510162384	40.0	37.0	41.0	33.0	41.0
16	37.791018235447744	40.0	36.0	41.0	33.0	41.0
17	37.731434255603524	39.0	36.0	41.0	33.0	41.0
18	37.615703685496136	39.0	36.0	41.0	33.0	41.0
19	37.63761848432243	39.0	36.0	41.0	32.0	41.0
20	37.622052294409535	39.0	36.0	41.0	32.0	41.0
21	37.58234372359547	39.0	36.0	41.0	32.0	41.0
22	37.555562810133196	39.0	36.0	41.0	32.0	41.0
23	37.43183214785	39.0	36.0	41.0	32.0	41.0
24	37.3536363357043	39.0	36.0	41.0	32.0	41.0
25	37.2673021676678	39.0	36.0	41.0	32.0	41.0
26	37.25401732905228	39.0	35.0	41.0	32.0	41.0
27	37.14284946153964	39.0	35.0	41.0	32.0	41.0
28	36.96300293426329	39.0	35.0	41.0	31.0	41.0
29	36.816620066673835	39.0	35.0	41.0	31.0	41.0
30	36.78935138955033	39.0	35.0	41.0	31.0	41.0
31	36.74436575361406	39.0	35.0	40.0	31.0	41.0
32	36.66123085431613	39.0	35.0	40.0	30.0	41.0
33	36.603467346719306	39.0	35.0	40.0	30.0	41.0
34	36.603905181816785	39.0	35.0	40.0	30.0	41.0
35	36.461578049867114	39.0	35.0	40.0	30.0	41.0
36	36.39528443918701	39.0	35.0	40.0	30.0	41.0
37	36.279292704284636	39.0	35.0	40.0	30.0	41.0
38	36.222785092098995	38.0	35.0	40.0	30.0	41.0
39	36.160090178667446	38.0	35.0	40.0	30.0	41.0
40	36.039259213740344	38.0	35.0	40.0	30.0	41.0
41	36.012101915720585	38.0	35.0	40.0	30.0	41.0
42	35.931144669933786	38.0	35.0	40.0	29.0	41.0
43	35.85710829121411	38.0	35.0	40.0	29.0	41.0
44	35.800792711965954	38.0	35.0	40.0	29.0	41.0
45	35.627037469466764	38.0	35.0	40.0	29.0	41.0
46	35.38559829782004	38.0	34.0	40.0	28.0	41.0
47	35.25808842732705	38.0	34.0	40.0	27.0	41.0
48	35.199656645107765	37.0	34.0	40.0	27.0	41.0
49	35.01366506383175	37.0	34.0	40.0	26.0	41.0
50	34.954280798242515	37.0	34.0	40.0	26.0	41.0
51	33.537423378857945	36.0	32.0	39.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	3.0
11	0.0
12	2.0
13	6.0
14	9.0
15	10.0
16	33.0
17	56.0
18	109.0
19	268.0
20	497.0
21	753.0
22	1030.0
23	1308.0
24	1564.0
25	1885.0
26	2067.0
27	2413.0
28	3059.0
29	3745.0
30	4957.0
31	6483.0
32	8792.0
33	11599.0
34	17630.0
35	22587.0
36	24556.0
37	35505.0
38	52250.0
39	57178.0
40	18.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	17.981196134761035	35.131657781942764	20.7145929669857	26.17255311631051
2	23.638870539074862	34.78331003333692	18.630267463475334	22.947551964112883
3	19.41529811193216	29.432120197256236	23.094649194229795	28.05793249658181
4	22.305777887023183	31.685818751632276	19.118415190573486	26.88998817077105
5	17.395111609543267	35.81337471003027	17.467315994039296	29.324197686387173
6	19.826632664034534	35.313705006682746	22.30385755764829	22.55580477163443
7	78.56605164917887	9.24254528136666	7.0222604581137755	5.169142611340697
8	79.8526723303581	5.7218134054353005	5.065060759221422	9.360453504985175
9	75.01574670087413	8.974083234756424	9.825557279584594	6.184612784784846
10	33.97216290538153	34.38503372098382	13.976157190481311	17.666646183153333
11	25.25002688461125	29.167114743520813	25.134423056242603	20.448435315625336
12	24.632833023520195	22.64874871337932	33.629576144900376	19.088842118200112
13	27.069346934386186	25.53385156622064	25.766211420582856	21.630590078810318
14	19.32312230193723	27.53253037961071	31.57597591138832	21.56837140706374
15	18.216628516123084	28.895196104035765	28.884826325411332	24.003349054429815
16	20.203785353263793	28.833361498164166	28.135897869202527	22.826955279369518
17	19.82816892753445	26.519364601416434	28.502680779807356	25.14978569124176
18	23.613906257201236	25.301875777733397	25.56457683621895	25.51964112884642
19	21.97048837816662	28.652466471049117	28.29221268031893	21.084832470465333
20	24.134699583672592	28.89327577466087	24.868649470757223	22.103375170909313
21	23.709538660070976	27.661576513603613	26.460218456669686	22.168666369655725
22	20.973069300846483	28.577573625428233	25.93251194444871	24.516845129276575
23	22.205536693653695	28.668597237798227	23.46872935645922	25.657136712088857
24	19.497872275052615	29.9145069362297	25.450125205475242	25.137495583242437
25	20.673881984237934	28.216551702948088	26.08114543806554	25.028420874748434
26	24.76418355276297	27.606655093481635	23.47564254220884	24.153518811546558
27	19.379195919684143	27.06896286851121	26.98446837601585	26.567372835788795
28	21.919791682669413	28.070222604581136	27.870124283717146	22.139861429032308
29	25.192801069239394	29.861889911357597	22.97328437773647	21.972024641666536
30	23.374249151214414	27.72494738297513	25.122517014118262	23.778286451692193
31	25.098320863994594	29.419061957506955	21.477347794693745	24.005269383804713
32	24.391639654033458	27.07433979076091	23.891201818935983	24.642818736269646
33	22.276588880524788	27.176885379380273	24.808351128385542	25.738174611709404
34	21.47619559706881	25.536540027345488	26.265112992180416	26.72215138340528
35	22.215522406403146	28.317945093942516	26.414898683422184	23.051633816232158
36	24.06326333092652	28.560290661054182	25.71436252746071	21.662083480558586
37	23.31548707234265	31.232621019157207	20.434993010001076	25.01689889849907
38	23.45874364370977	29.802743766610853	24.270658903415114	22.46785368626427
39	25.024196150123668	24.111271565298882	23.609681532576467	27.25485075200098
40	23.853563363188055	26.772848078902495	23.548615058454825	25.82497349945463
41	20.37239027237952	24.18424408154487	28.053323706082068	27.390041939993548
42	23.688415036947134	26.814711259275192	24.345167683161016	25.151706020616654
43	22.250088335151244	25.287665340359176	27.02633155638855	25.435914768101025
44	22.632233880755226	23.25595686172092	25.27307083710998	28.83873842041387
45	27.38850567649363	23.987218287680705	24.892461555005916	23.73181448081975
46	22.20745702302859	22.970979982486597	30.54053431244527	24.281028682039544
47	21.535725807690532	26.49555251716774	24.81872090700997	27.15000076813175
48	20.63509133086507	24.093220469174874	29.982102530225983	25.289585669734073
49	23.878143579186702	24.23417264529212	27.199161200129048	24.68852257539213
50	21.713164241930777	22.810824512620403	26.874625535771894	28.601385709676926
51	20.745318236984005	22.02656199591354	30.528628270320922	26.69949149678153
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1721.0
1	1331.0
2	941.0
3	621.0
4	301.0
5	283.0
6	265.0
7	287.0
8	309.0
9	355.0
10	401.0
11	423.0
12	445.0
13	459.5
14	474.0
15	489.5
16	505.0
17	623.0
18	741.0
19	730.5
20	720.0
21	741.0
22	762.0
23	941.5
24	1121.0
25	1237.0
26	1533.5
27	1714.0
28	2069.0
29	2424.0
30	2952.5
31	3481.0
32	4234.5
33	4988.0
34	4637.0
35	4286.0
36	5087.5
37	5889.0
38	6702.0
39	7515.0
40	7738.5
41	7962.0
42	10128.0
43	12294.0
44	17729.5
45	23165.0
46	23467.0
47	23769.0
48	24955.5
49	26142.0
50	25249.5
51	24357.0
52	22091.5
53	19826.0
54	17847.5
55	15869.0
56	15064.0
57	14259.0
58	13564.0
59	12869.0
60	12006.5
61	11144.0
62	9634.0
63	8124.0
64	7011.5
65	5899.0
66	5121.5
67	4344.0
68	3873.0
69	3402.0
70	2709.0
71	2016.0
72	1787.5
73	1559.0
74	1340.5
75	917.0
76	712.0
77	658.0
78	604.0
79	467.0
80	330.0
81	261.0
82	192.0
83	112.5
84	33.0
85	21.5
86	10.0
87	6.5
88	3.0
89	4.0
90	5.0
91	4.0
92	3.0
93	2.5
94	2.0
95	1.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	260372.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	29.599188852872043
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.0842891991488	23.112316224478825
2	9.150360720402762	5.416865100702072
3	3.124513416722894	2.7744918808474033
4	1.483105828618882	1.7559491804034228
5	0.8602792339232884	1.273178375554975
6	0.6215290392899777	1.1038053246892907
7	0.5423781595474126	1.1237767501881923
8	0.3659106243836612	0.8664526139523451
9	0.34514973789380804	0.91945370469943
>10	4.301396169616443	30.967231499546806
>50	0.9978201069185655	19.816262885410104
>100	0.1115897648829605	5.260934355460649
>500	0.00519022162246328	0.9052432673252117
>1k	0.006487777028079098	4.704038836741278
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AATGATACGGCTGTCTCTTATACACATCTGACGCTGAAGCCATCGTATGCC	2956	1.1352987264375587	No Hit
AATGATACCTGTCTCTTATACACATCTGACGCTGAAGCCATCGTATGCCGT	2928	1.12454488193815	No Hit
CTGTCTCTTATACACATCTGACGCTGAAGCCATCGTATGCCGTCTTCTGCT	2585	0.9928102868203954	No Hit
AATCTGTCTCTTATACACATCTGACGCTGAAGCCATCGTATGCCGTCTTCT	2288	0.8787427219516691	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1491	0.5726422195935047	No Hit
AATGACTGTCTCTTATACACATCTGACGCTGAAGCCATCGTATGCCGTCTT	685	0.26308512436053033	No Hit
AAACTGTCTCTTATACACATCTGACGCTGAAGCCATCGTATGCCGTCTTCT	589	0.2262148003625582	No Hit
AATGATCTGTCTCTTATACACATCTGACGCTGAAGCCATCGTATGCCGTCT	559	0.21469282411319188	No Hit
TGCAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGG	524	0.20125051848893122	No Hit
AATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCTGAAGCCATC	494	0.18972854223956492	No Hit
TCCCCCGGGAATGATACCTGTCTCTTATACACATCTGACGCTGAAGCCATC	457	0.17551810486534652	No Hit
AACTGTCTCTTATACACATCTGACGCTGAAGCCATCGTATGCCGTCTTCTG	450	0.17282964374049437	No Hit
AATGATACGGCGACTGTCTCTTATACACATCTGACGCTGAAGCCATCGTAT	422	0.16207579924108584	No Hit
AAAAACTGTCTCTTATACACATCTGACGCTGAAGCCATCGTATGCCGTCTT	403	0.15477854761648718	No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	368	0.1413362419922265	No Hit
CCTGTCTCTTATACACATCTGACGCTGAAGCCATCGTATGCCGTCTTCTGC	329	0.12635767286805033	No Hit
TCCCCCGGGAATGATACGGCTGTCTCTTATACACATCTGACGCTGAAGCCA	296	0.1136834989937474	No Hit
CGCAACGGGAGAAACAAGCGAGATAGGAATGTCTTACACGCGGGGCAAGAC	268	0.10292965449433887	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	262	0.10062525924446561	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	1.1145591691886994	0.0
2	0.0	0.0	0.0	1.4129783540472862	0.0
3	0.0	0.0	0.0	1.9053508057702058	0.0
4	0.0	0.0	0.0	4.465917994254374	0.0
5	0.0	0.0	0.0	4.838077827108906	0.0
6	0.0	0.0	0.0	6.413516046272257	0.0
7	0.0	0.0	0.0	7.628700474705422	0.0
8	0.0	0.0	0.0	8.427557494661484	0.0
9	0.0	0.0	0.0	10.563347825419015	0.0
10	0.0	0.0	0.0	12.088089349085156	0.0
11	0.0	0.0	0.0	14.518074140076505	0.0
12	0.0	0.0	0.0	15.38836741277864	0.0
13	0.0	0.0	0.0	15.810839875255404	0.0
14	0.0	0.0	0.0	16.35198869310064	0.0
15	0.0	0.0	0.0	16.72222819658028	0.0
16	0.0	0.0	0.0	17.41930775966694	0.0
17	0.0	0.0	0.0	18.21432412087321	0.0
18	0.0	0.0	0.0	19.86311892215753	0.0
19	0.0	0.0	0.0	20.301722151383405	0.0
20	0.0	0.0	0.0	20.931590186348764	0.0
21	0.0	0.0	0.0	21.41436099119721	0.0
22	0.0	0.0	0.0	21.94091530579325	0.0
23	0.0	0.0	0.0	22.457099841764858	0.0
24	0.0	0.0	0.0	22.773954188622433	0.0
25	0.0	0.0	0.0	23.104634906979246	0.0
26	0.0	0.0	0.0	23.37194475596454	0.0
27	0.0	0.0	0.0	23.797489745441137	0.0
28	0.0	0.0	0.0	24.145453428172	0.0
29	0.0	0.0	0.0	24.443104481280628	0.0
30	0.0	0.0	0.0	25.005760988124685	0.0
31	0.0	0.0	0.0	25.316470280982593	0.0
32	0.0	0.0	0.0	25.65905704146375	0.0
33	0.0	0.0	0.0	25.987049298695712	0.0
34	0.0	0.0	0.0	26.283164088304424	0.0
35	0.0	0.0	0.0	26.629591507535373	0.0
36	0.0	0.0	0.0	26.89805355414561	0.0
37	0.0	0.0	0.0	27.210683176378414	0.0
38	0.0	0.0	0.0	27.50564576836219	0.0
39	0.0	0.0	0.0	27.836326486719003	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CATGCGG	45	3.8198777E-10	45.000004	2
TACCTAG	65	0.0	45.000004	1
TCAAACG	45	3.8198777E-10	45.000004	1
TATCATG	25	3.882468E-5	45.0	1
CTTAACG	20	7.0232264E-4	45.0	1
TGTCATA	20	7.0232264E-4	45.0	45
CAGGTAG	20	7.0232264E-4	45.0	20
ACTATAG	25	3.882468E-5	45.0	1
ATCTCAA	20	7.0232264E-4	45.0	18
GGTACAA	25	3.882468E-5	45.0	8
CAAACGG	55	1.8189894E-12	45.0	2
CGAACCG	20	7.0232264E-4	45.0	1
GCGACTC	25	3.882468E-5	45.0	43
CTACTGA	20	7.0232264E-4	45.0	13
CGTATAG	20	7.0232264E-4	45.0	1
CTGTAAG	20	7.0232264E-4	45.0	14
CGAAATA	20	7.0232264E-4	45.0	11
CGAGTGC	20	7.0232264E-4	45.0	31
CGACGTT	20	7.0232264E-4	45.0	24
ACGCATG	20	7.0232264E-4	45.0	1
>>END_MODULE