##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3548445_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	605875
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.589859294408914	31.0	31.0	34.0	30.0	34.0
2	31.789187538683723	31.0	31.0	34.0	30.0	34.0
3	31.93485289870023	33.0	31.0	34.0	30.0	34.0
4	35.61419764802971	37.0	35.0	37.0	33.0	37.0
5	35.37320734474933	37.0	35.0	37.0	33.0	37.0
6	35.39036104807097	37.0	35.0	37.0	33.0	37.0
7	35.38090860325975	37.0	35.0	37.0	33.0	37.0
8	35.48759232514958	37.0	35.0	37.0	33.0	37.0
9	37.14376067670724	39.0	37.0	39.0	34.0	39.0
10	36.741565504435734	39.0	37.0	39.0	32.0	39.0
11	36.78878316484423	39.0	37.0	39.0	32.0	39.0
12	36.63324943263875	39.0	35.0	39.0	32.0	39.0
13	36.665237879100474	39.0	35.0	39.0	32.0	39.0
14	37.70324406849598	39.0	36.0	41.0	33.0	41.0
15	37.799650092840935	39.0	37.0	41.0	33.0	41.0
16	37.60690076335878	39.0	36.0	41.0	32.0	41.0
17	37.57147431400867	39.0	36.0	41.0	32.0	41.0
18	37.434249638951925	39.0	36.0	41.0	32.0	41.0
19	37.43744996905302	39.0	36.0	41.0	32.0	41.0
20	37.46515535382711	39.0	36.0	41.0	32.0	41.0
21	37.447833298947806	39.0	36.0	41.0	32.0	41.0
22	37.403608004951515	39.0	36.0	41.0	32.0	41.0
23	37.25115411594801	39.0	36.0	41.0	32.0	41.0
24	37.14731256447287	39.0	35.0	41.0	31.0	41.0
25	37.15311574169589	39.0	35.0	41.0	31.0	41.0
26	37.12928409325356	39.0	36.0	41.0	31.0	41.0
27	37.01986218279348	39.0	35.0	40.0	31.0	41.0
28	36.8909577057974	39.0	35.0	40.0	31.0	41.0
29	36.73066061481329	39.0	35.0	40.0	31.0	41.0
30	36.7409465648855	39.0	35.0	40.0	30.0	41.0
31	36.723789560552916	39.0	35.0	40.0	31.0	41.0
32	36.63362739839076	39.0	35.0	40.0	30.0	41.0
33	36.63190096967196	39.0	35.0	40.0	30.0	41.0
34	36.578137404580154	39.0	35.0	40.0	30.0	41.0
35	36.468184031359606	39.0	35.0	40.0	30.0	41.0
36	36.46759975242418	39.0	35.0	40.0	30.0	41.0
37	36.33214772023933	39.0	35.0	40.0	30.0	41.0
38	36.3852065194966	39.0	35.0	40.0	30.0	41.0
39	36.29084382092016	39.0	35.0	40.0	30.0	41.0
40	36.09223519702909	38.0	35.0	40.0	30.0	41.0
41	36.095136785640605	38.0	35.0	40.0	30.0	41.0
42	36.01547183825046	38.0	35.0	40.0	30.0	41.0
43	35.97262636682484	38.0	35.0	40.0	30.0	41.0
44	35.87217495357953	38.0	35.0	40.0	29.0	41.0
45	35.66425417784196	38.0	35.0	40.0	28.0	41.0
46	35.46500350732412	38.0	34.0	40.0	28.0	41.0
47	35.363225087683105	38.0	34.0	40.0	27.0	41.0
48	35.287116979574996	38.0	34.0	40.0	27.0	41.0
49	35.08998555807716	37.0	34.0	40.0	27.0	41.0
50	35.01617990509594	37.0	34.0	40.0	26.0	41.0
51	33.483840726222404	36.0	31.0	39.0	22.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	3.0
9	6.0
10	3.0
11	5.0
12	1.0
13	12.0
14	10.0
15	17.0
16	61.0
17	113.0
18	270.0
19	530.0
20	1072.0
21	1749.0
22	2317.0
23	2873.0
24	3319.0
25	3906.0
26	4767.0
27	5763.0
28	7372.0
29	9587.0
30	12271.0
31	16284.0
32	21387.0
33	28589.0
34	42859.0
35	52071.0
36	58913.0
37	81276.0
38	120756.0
39	127682.0
40	31.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	18.338766247163193	37.91491644316072	18.389931916649473	25.356385393026613
2	21.487600577676915	35.781803177223026	19.988446461728905	22.742149783371158
3	20.200041262636685	26.74611099649268	26.176851660821125	26.876996080049516
4	23.971941407055912	29.434124200536417	18.3740870641634	28.219847328244274
5	16.64650299154116	36.66630905714875	18.11561790798432	28.571570043325767
6	20.825582834743138	33.660573550649886	23.71429750361048	21.79954611099649
7	75.31107901794924	10.309882401485455	8.166205900557046	6.212832680008253
8	76.88483598101918	4.960264080874768	6.231483391788736	11.92341654631731
9	72.58675469362493	8.51512275634413	11.738559933979781	7.159562616051166
10	33.2438209201568	33.13538271095523	15.309428512481949	18.311367856406026
11	24.277449969053023	26.852403548586757	27.502537652155972	21.367608830204247
12	24.92510831442129	21.899071590674644	35.56492675881989	17.610893336084178
13	26.775820094904063	24.840602434495565	26.025005157829582	22.358572312770786
14	17.528368062719206	28.376315246544255	33.39484217041469	20.700474520321848
15	16.914710129977305	28.726882607798636	29.114091190427065	25.24431607179699
16	19.07010521972354	28.59946358572313	29.617495357953374	22.71293583659996
17	18.287270476583455	26.674479059211887	28.598803383536207	26.439447080668455
18	21.90451825871673	26.547060037136372	25.993975655044355	25.554446049102538
19	20.92873942644935	29.93719826696926	29.424056117185888	19.7100061893955
20	22.748256653600166	28.714998968434085	26.441757788322672	22.094986589643078
21	21.671466886734063	27.18035898493914	26.793645553950896	24.3545285743759
22	19.778337115741696	27.917639777181762	27.25281617495358	25.05120693212296
23	22.199628636269857	26.408582628429954	24.08170002063132	27.31008871466887
24	18.005694243862184	31.590839694656488	25.069692593356713	25.333773468124615
25	20.59170621002682	26.131957912110582	25.83008046214153	27.446255415721065
26	23.414730761295647	26.621662884258303	24.212585104188157	25.751021250257892
27	17.278481534970084	25.25009284093254	29.375366205900555	28.096059418196823
28	22.253600165050546	24.736950691149165	26.596410150608623	26.413038993191666
29	23.697462347844027	28.089457396327628	24.692056942438622	23.521023313389726
30	21.015060862389106	23.102125025789146	28.36905302248814	27.51376108933361
31	24.218691974417165	26.2737363317516	25.385599339797814	24.12197235403342
32	22.09086032597483	23.40581803177223	24.66961006808335	29.83371157416959
33	22.158365999587375	23.32906952754281	26.3790385805653	28.13352589230452
34	20.348091603053437	22.76492675881989	29.484629667835776	27.4023519702909
35	20.446791829997938	25.19612131215185	27.19620383742521	27.160883020425004
36	22.181638126676294	24.404208788941613	31.784113884877247	21.63003919950485
37	20.017165256859915	26.481369919537855	26.89317103362905	26.60829378997318
38	21.483144212915207	25.470930472457194	28.414937074479056	24.630988240148547
39	21.036022281823808	21.85846915617908	28.948050340416753	28.157458221580363
40	21.96014029296472	26.744130389931918	25.033546523622856	26.262182793480505
41	19.014648236022282	23.02009490406437	32.68991128533113	25.275345574582214
42	23.100804621415307	25.789973179286157	24.85100061893955	26.25822158035899
43	21.805487930678773	21.851537033216424	29.18506292552094	27.157912110583865
44	21.606932122962657	19.68491850629255	28.946069733855996	29.7620796368888
45	26.076005776769133	22.137569630699403	27.667423148339175	24.119001444192286
46	18.720693212296265	21.56731999174747	33.809779244893754	25.90220755106251
47	21.9319166494739	24.22760470394058	25.75696306994017	28.083515576645347
48	20.212750154734888	20.993769341860947	34.55184650299154	24.241634000412628
49	22.76806271920776	21.533814730761293	28.663008046214156	27.035114503816793
50	20.686775324943262	20.132865690117598	28.33043119455333	30.849927790385806
51	20.162079636888798	19.09205694243862	33.86193521766041	26.883928203012175
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	2564.0
1	2021.0
2	1478.0
3	979.0
4	480.0
5	466.5
6	453.0
7	476.5
8	500.0
9	529.5
10	559.0
11	625.5
12	692.0
13	701.5
14	711.0
15	829.0
16	947.0
17	1017.0
18	1087.0
19	1293.0
20	1499.0
21	1343.0
22	1187.0
23	1797.0
24	2407.0
25	2936.5
26	3824.5
27	4183.0
28	5801.0
29	7419.0
30	8158.5
31	8898.0
32	9779.5
33	10661.0
34	11234.0
35	11807.0
36	13231.5
37	14656.0
38	15636.0
39	16616.0
40	18520.5
41	20425.0
42	23541.5
43	26658.0
44	44904.0
45	63150.0
46	63796.0
47	64442.0
48	65291.5
49	66141.0
50	59861.0
51	53581.0
52	49238.0
53	44895.0
54	40604.0
55	36313.0
56	32989.5
57	29666.0
58	27166.0
59	24666.0
60	23722.0
61	22778.0
62	20313.0
63	17848.0
64	15906.5
65	13965.0
66	11737.5
67	9510.0
68	8265.0
69	7020.0
70	5644.5
71	4269.0
72	3691.0
73	3113.0
74	2566.5
75	1851.5
76	1683.0
77	1156.5
78	630.0
79	448.0
80	266.0
81	295.5
82	325.0
83	203.5
84	82.0
85	58.0
86	34.0
87	60.5
88	87.0
89	59.5
90	32.0
91	17.5
92	3.0
93	2.5
94	2.0
95	1.0
96	0.0
97	0.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	605875.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	25.932121424125004
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.34734457778227	19.539164885764368
2	10.505182038427623	5.448433124060843
3	4.054588054808633	3.154322092863129
4	2.0182619596807467	2.0935125681653446
5	1.1896373656145787	1.5424910307896724
6	0.7661259464623492	1.1920362641900601
7	0.5551243556968349	1.0076886538193637
8	0.3961448237330322	0.821830053646687
9	0.313061811799584	0.7306521225159045
>10	3.35307399150232	20.73346476884336
>50	1.141157144575534	21.232708894648322
>100	0.34747593952805583	13.298061457660651
>500	0.005769895674820115	1.0445531847087297
>1k	0.0044876966359712	2.164417939156924
>5k	0.0012821990388489145	2.613959847712137
>10k+	0.0012821990388489145	3.382703111454484
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AATGATACCTGTCTCTTATACACATCTGACGCCGTTCCTATCGTATGCCGT	10294	1.6990303280379617	No Hit
AATGATACGGCTGTCTCTTATACACATCTGACGCCGTTCCTATCGTATGCC	10053	1.659253146276047	No Hit
AATCTGTCTCTTATACACATCTGACGCCGTTCCTATCGTATGCCGTCTTCT	9258	1.528037961625748	No Hit
CTGTCTCTTATACACATCTGACGCCGTTCCTATCGTATGCCGTCTTCTGCT	6465	1.0670517846090366	TruSeq Adapter, Index 16 (95% over 23bp)
AATGACTGTCTCTTATACACATCTGACGCCGTTCCTATCGTATGCCGTCTT	2587	0.4269857643903445	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2128	0.3512275634413039	No Hit
AATGATCTGTCTCTTATACACATCTGACGCCGTTCCTATCGTATGCCGTCT	2042	0.3370332164225294	No Hit
AACTGTCTCTTATACACATCTGACGCCGTTCCTATCGTATGCCGTCTTCTG	1816	0.2997317928615639	No Hit
AATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCCGTTCCTATC	1592	0.2627604703940582	No Hit
AATGATACGGCGACTGTCTCTTATACACATCTGACGCCGTTCCTATCGTAT	1530	0.25252733649680215	No Hit
AAACTGTCTCTTATACACATCTGACGCCGTTCCTATCGTATGCCGTCTTCT	1324	0.21852692387043532	No Hit
CCTGTCTCTTATACACATCTGACGCCGTTCCTATCGTATGCCGTCTTCTGC	990	0.16340004126263669	TruSeq Adapter, Index 13 (95% over 21bp)
AAAAACTGTCTCTTATACACATCTGACGCCGTTCCTATCGTATGCCGTCTT	797	0.13154528574375904	No Hit
AGCCCAGGGAATGATACCTGTCTCTTATACACATCTGACGCCGTTCCTATC	784	0.12939962863626986	No Hit
AATGCTGTCTCTTATACACATCTGACGCCGTTCCTATCGTATGCCGTCTTC	756	0.12477821332783165	No Hit
TGCAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGG	679	0.11206932122962657	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.6505054672993604E-4	0.0	0.0	1.205199092221993	0.0
2	3.301010934598721E-4	0.0	0.0	1.6006602021869198	0.0
3	3.301010934598721E-4	0.0	0.0	2.341076954817413	0.0
4	3.301010934598721E-4	0.0	0.0	5.760594181968227	0.0
5	3.301010934598721E-4	0.0	0.0	6.16727872911079	0.0
6	3.301010934598721E-4	0.0	0.0	8.058592944089128	0.0
7	3.301010934598721E-4	0.0	0.0	9.459872085826284	0.0
8	3.301010934598721E-4	0.0	0.0	10.412213740458014	0.0
9	3.301010934598721E-4	0.0	0.0	13.361997111615432	0.0
10	3.301010934598721E-4	0.0	0.0	15.389312977099237	0.0
11	3.301010934598721E-4	0.0	0.0	18.592448937487106	0.0
12	3.301010934598721E-4	0.0	0.0	19.551392613988035	0.0
13	3.301010934598721E-4	0.0	0.0	20.07674850422942	0.0
14	3.301010934598721E-4	0.0	0.0	20.736455539508974	0.0
15	3.301010934598721E-4	0.0	0.0	21.185227976067672	0.0
16	3.301010934598721E-4	0.0	0.0	22.026655663296886	0.0
17	3.301010934598721E-4	0.0	0.0	22.948297916236847	0.0
18	3.301010934598721E-4	0.0	0.0	24.759562616051166	0.0
19	3.301010934598721E-4	0.0	0.0	25.271549412007428	0.0
20	3.301010934598721E-4	0.0	0.0	26.050587992572726	0.0
21	3.301010934598721E-4	0.0	0.0	26.593769341860945	0.0
22	3.301010934598721E-4	0.0	0.0	27.18002888384568	0.0
23	3.301010934598721E-4	0.0	0.0	27.72040437383949	0.0
24	3.301010934598721E-4	0.0	0.0	28.10216628842583	0.0
25	3.301010934598721E-4	0.0	0.0	28.43606354446049	0.0
26	3.301010934598721E-4	0.0	0.0	28.7691355477615	0.0
27	3.301010934598721E-4	0.0	0.0	29.218733237053847	0.0
28	3.301010934598721E-4	0.0	0.0	29.607097173509388	0.0
29	3.301010934598721E-4	0.0	0.0	29.98407262224056	0.0
30	3.301010934598721E-4	0.0	0.0	30.623973591912524	0.0
31	3.301010934598721E-4	0.0	0.0	30.94763771404993	0.0
32	3.301010934598721E-4	0.0	0.0	31.33187538683722	0.0
33	3.301010934598721E-4	0.0	0.0	31.69152052816175	0.0
34	3.301010934598721E-4	0.0	0.0	32.02805859294409	0.0
35	3.301010934598721E-4	0.0	0.0	32.40404373839488	0.0
36	3.301010934598721E-4	0.0	0.0	32.74668867340623	0.0
37	3.301010934598721E-4	0.0	0.0	33.07744996905302	0.0
38	3.301010934598721E-4	0.0	0.0	33.4054054054054	0.0
39	3.301010934598721E-4	0.0	0.0	33.73220548793068	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACGCGAC	35	1.2106648E-7	45.000004	45
ACAACGT	30	2.1637952E-6	45.000004	38
GTACCAT	20	7.030642E-4	45.000004	12
GCGACTC	20	7.030642E-4	45.000004	23
CTCGTGG	20	7.030642E-4	45.000004	30
CGAAACG	20	7.030642E-4	45.000004	1
CCTAGCG	40	6.8066583E-9	45.000004	1
AACCCGA	20	7.030642E-4	45.000004	24
GGCCGTA	20	7.030642E-4	45.000004	42
ATAGCGC	20	7.030642E-4	45.000004	1
ACCGAGT	20	7.030642E-4	45.000004	20
TTCGTTC	20	7.030642E-4	45.000004	42
GCGTTCG	20	7.030642E-4	45.000004	39
CGTTCGC	20	7.030642E-4	45.000004	40
TCGGCAA	20	7.030642E-4	45.000004	42
AATATCG	20	7.030642E-4	45.000004	24
ACCTAGA	30	2.1637952E-6	45.000004	23
TCGGACG	20	7.030642E-4	45.000004	26
TATCTCG	20	7.030642E-4	45.000004	1
CGTACTC	20	7.030642E-4	45.000004	45
>>END_MODULE