SRR3548443_1.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR3548443_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	356900
Sequences flagged as poor quality	0
Sequence length	51
%GC	46

Per base sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
AATGATACCTGTCTCTTATACACATCTGACGCCCACCATTTCGTATGCCGT	10308	2.8882039787055196	No Hit
AATGATACGGCTGTCTCTTATACACATCTGACGCCCACCATTTCGTATGCC	9987	2.7982628187167276	No Hit
AATCTGTCTCTTATACACATCTGACGCCCACCATTTCGTATGCCGTCTTCT	8749	2.4513869431213227	RNA PCR Primer, Index 14 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCCCACCATTTCGTATGCCGTCTTCTGCT	4888	1.3695713084897732	Illumina PCR Primer Index 11 (95% over 23bp)
AATGACTGTCTCTTATACACATCTGACGCCCACCATTTCGTATGCCGTCTT	2429	0.680582796301485	No Hit
CACCCAGGGAATGATACCTGTCTCTTATACACATCTGACGCCCACCATTTC	2008	0.5626225833566825	No Hit
AATGATCTGTCTCTTATACACATCTGACGCCCACCATTTCGTATGCCGTCT	1893	0.530400672457271	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1882	0.5273185766321098	No Hit
AACTGTCTCTTATACACATCTGACGCCCACCATTTCGTATGCCGTCTTCTG	1629	0.45643037265340436	Illumina PCR Primer Index 11 (95% over 21bp)
AATGATACGGCGACTGTCTCTTATACACATCTGACGCCCACCATTTCGTAT	1531	0.4289717007565144	No Hit
AATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCCCACCATTTC	1437	0.40263379097786495	No Hit
CACCCAGGGAATGATACGGCTGTCTCTTATACACATCTGACGCCCACCATT	1245	0.3488372093023256	No Hit
AAACTGTCTCTTATACACATCTGACGCCCACCATTTCGTATGCCGTCTTCT	1041	0.29167834127206504	RNA PCR Primer, Index 14 (95% over 21bp)
CACCCAGGGAATGATCTGTCTCTTATACACATCTGACGCCCACCATTTCGT	882	0.24712804707200897	No Hit
CACCTGTCTCTTATACACATCTGACGCCCACCATTTCGTATGCCGTCTTCT	836	0.23423928271224434	RNA PCR Primer, Index 14 (95% over 21bp)
AAAAACTGTCTCTTATACACATCTGACGCCCACCATTTCGTATGCCGTCTT	795	0.2227514710002802	No Hit
CACCCAGGGAACTGTCTCTTATACACATCTGACGCCCACCATTTCGTATGC	774	0.21686746987951808	No Hit
CCTGTCTCTTATACACATCTGACGCCCACCATTTCGTATGCCGTCTTCTGC	682	0.19108994115998879	Illumina PCR Primer Index 11 (95% over 22bp)
AATGCTGTCTCTTATACACATCTGACGCCCACCATTTCGTATGCCGTCTTC	634	0.17764079574110395	No Hit
AATGATACGGCTGTCTCTTATACACATCTGACGCCCACCCTTTCGTATGCC	488	0.13673297842532922	No Hit
AAAAAACTGTCTCTTATACACATCTGACGCCCACCATTTCGTATGCCGTCT	444	0.12440459512468478	No Hit
AATGATACGGCCTGTCTCTTATACACATCTGACGCCCACCATTTCGTATGC	442	0.12384421406556458	No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	429	0.12020173718128328	No Hit
AATGATACGGCGCTGTCTCTTATACACATCTGACGCCCACCATTTCGTATG	418	0.11711964135612217	No Hit
ACCTGTCTCTTATACACATCTGACGCCCACCATTTCGTATGCCGTCTTCTG	399	0.11179602129448026	RNA PCR Primer, Index 14 (95% over 22bp)
TACCTGTCTCTTATACACATCTGACGCCCACCATTTCGTATGCCGTCTTCT	382	0.10703278229195853	No Hit
CACCCAGGGCTGTCTCTTATACACATCTGACGCCCACCATTTCGTATGCCG	380	0.10647240123283833	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTTAACG	25	3.8853825E-5	45.000004	1
CGGGTAG	25	3.8853825E-5	45.000004	6
TACCCTT	25	3.8853825E-5	45.000004	35
GTACCAC	25	3.8853825E-5	45.000004	23
GTTCTCG	25	3.8853825E-5	45.000004	24
CTCAACG	25	3.8853825E-5	45.000004	1
GCGCGTA	25	3.8853825E-5	45.000004	11
GTCCTTC	25	3.8853825E-5	45.000004	30
TTTAACG	25	3.8853825E-5	45.000004	1
CCCAGCG	25	3.8853825E-5	45.000004	1
TACGGGT	25	3.8853825E-5	45.000004	4
CGCGACG	25	3.8853825E-5	45.000004	1
TTCCCGG	25	3.8853825E-5	45.000004	2
CTAGTCG	25	3.8853825E-5	45.000004	1
TGCGAGG	25	3.8853825E-5	45.000004	2
AGCGCGG	50	2.1827873E-11	45.000004	2
CGTAATA	25	3.8853825E-5	45.000004	40
TTTGCGC	25	3.8853825E-5	45.000004	13
ACGTAAT	25	3.8853825E-5	45.000004	39
ATACGTA	25	3.8853825E-5	45.000004	37

Summary

Basic Statistics

Per base sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content