##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3548443_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	356900
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.387525917623986	31.0	31.0	34.0	30.0	34.0
2	31.591711964135612	31.0	31.0	34.0	30.0	34.0
3	31.737097226113757	31.0	31.0	34.0	30.0	34.0
4	35.41710002801905	37.0	35.0	37.0	33.0	37.0
5	35.14959092182684	37.0	35.0	37.0	32.0	37.0
6	35.14572709442421	37.0	35.0	37.0	32.0	37.0
7	35.109621742785095	37.0	35.0	37.0	32.0	37.0
8	35.32126365928831	37.0	35.0	37.0	33.0	37.0
9	36.933398711123566	39.0	37.0	39.0	33.0	39.0
10	36.48596805827963	38.0	35.0	39.0	32.0	39.0
11	36.56089941159989	38.0	35.0	39.0	32.0	39.0
12	36.50283272625385	38.0	35.0	39.0	32.0	39.0
13	36.56567385822359	38.0	35.0	39.0	32.0	39.0
14	37.51301485009807	39.0	36.0	40.0	32.0	41.0
15	37.681650322219106	39.0	36.0	41.0	33.0	41.0
16	37.380453908657884	39.0	36.0	40.0	32.0	41.0
17	37.32761277668815	39.0	36.0	40.0	32.0	41.0
18	37.14420846175399	39.0	36.0	40.0	31.0	41.0
19	37.07555057439058	39.0	35.0	40.0	31.0	41.0
20	37.20066405155506	39.0	36.0	40.0	32.0	41.0
21	37.21695152703838	39.0	36.0	40.0	32.0	41.0
22	37.15065844774447	39.0	36.0	40.0	31.0	41.0
23	36.930997478285235	39.0	35.0	40.0	31.0	41.0
24	36.892899971980945	39.0	35.0	40.0	31.0	41.0
25	36.845550574390586	39.0	35.0	40.0	31.0	41.0
26	36.832984029139816	39.0	35.0	40.0	31.0	41.0
27	36.705009806668535	39.0	35.0	40.0	30.0	41.0
28	36.54449425609415	39.0	35.0	40.0	30.0	41.0
29	36.39369010927431	39.0	35.0	40.0	30.0	41.0
30	36.375021014289715	39.0	35.0	40.0	30.0	41.0
31	36.35913981507425	39.0	35.0	40.0	30.0	41.0
32	36.21293359484449	38.0	35.0	40.0	30.0	41.0
33	36.036957130848975	38.0	35.0	40.0	29.0	41.0
34	36.17390025217148	39.0	35.0	40.0	30.0	41.0
35	35.99262818716728	38.0	35.0	40.0	29.0	41.0
36	36.11251330905015	39.0	35.0	40.0	30.0	41.0
37	35.912616979546094	38.0	35.0	40.0	28.0	41.0
38	35.80666012888764	38.0	35.0	40.0	28.0	41.0
39	35.73650322219109	38.0	35.0	40.0	28.0	41.0
40	35.285906416363126	38.0	34.0	40.0	26.0	41.0
41	35.5323564023536	38.0	35.0	40.0	27.0	41.0
42	35.55032221910899	38.0	35.0	40.0	27.0	41.0
43	35.62257495096666	38.0	35.0	40.0	28.0	41.0
44	35.513533202577754	38.0	35.0	40.0	27.0	41.0
45	35.28202857943401	38.0	34.0	40.0	26.0	41.0
46	35.09151583076492	38.0	34.0	40.0	26.0	41.0
47	34.868346875875595	38.0	34.0	40.0	24.0	41.0
48	34.89918744746428	38.0	34.0	40.0	25.0	41.0
49	34.70862706640516	38.0	33.0	40.0	24.0	41.0
50	34.72118520594004	38.0	33.0	40.0	24.0	41.0
51	33.09755113477164	36.0	31.0	39.0	20.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	0.0
11	2.0
12	4.0
13	5.0
14	7.0
15	6.0
16	40.0
17	79.0
18	198.0
19	402.0
20	741.0
21	1224.0
22	1835.0
23	2320.0
24	2658.0
25	3000.0
26	3263.0
27	3837.0
28	4786.0
29	6392.0
30	8351.0
31	11150.0
32	14406.0
33	18779.0
34	24746.0
35	31234.0
36	35830.0
37	48028.0
38	68067.0
39	65499.0
40	10.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	15.120201737181283	41.12692630989073	17.42084617539927	26.332025777528717
2	18.518072289156624	43.74782852339591	17.202577752871953	20.531521434575513
3	17.681423367890165	27.59092182684225	29.594284113196974	25.133370692070606
4	25.474082376015687	27.217988231997758	17.0098066685346	30.298122723451947
5	14.575231157186888	39.3552815914822	18.185486130568787	27.884001120762118
6	18.74838890445503	34.20818156346315	26.861025497338193	20.182404034743627
7	69.44606332305968	14.300644438217988	9.951527038386104	6.301765200336229
8	71.76071728775567	5.247968618660689	6.342953208181563	16.648360885402074
9	70.52003362286354	8.840011207621181	12.928831605491734	7.711123564023536
10	27.725693471560664	40.035023816195014	15.130008405715886	17.109274306528437
11	18.309050154104792	27.099747828523395	34.19529279910339	20.395909218268425
12	20.072008966096945	21.00168114317736	41.608573830204534	17.317736060521156
13	25.86382740263379	26.506864667974224	26.544690389464836	21.08461753992715
14	14.898010647240124	29.043149341552255	37.28747548332866	18.77136452787896
15	13.88736340711684	31.04735219949566	29.597646399551696	25.467637993835808
16	16.068646679742223	31.52787895769123	30.65004202857943	21.75343233398711
17	14.157186887083217	29.09106192210703	30.255533762958812	26.496217427850937
18	18.121042308769965	26.873073690109273	27.375455309610537	27.63042869151023
19	18.541047912580556	31.13813393107313	33.107593163351076	17.213224992995237
20	18.292518912860743	32.926590081255256	27.350798543009248	21.430092462874757
21	18.60437097226114	27.180722891566266	29.85990473521995	24.35500140095265
22	16.9417203698515	31.455029420005605	29.196973942280753	22.406276267862147
23	20.267862146259457	26.510226954328942	25.334547492294764	27.887363407116837
24	13.353880638834406	35.2787895769123	26.130008405715888	25.237321378537402
25	17.33034463435136	27.295601008685903	27.92490893807789	27.44914541888484
26	20.669935556178203	29.5990473521995	23.24292518912861	26.488091902493693
27	13.932754272905576	29.004763239002525	28.111515830764922	28.950966657326983
28	19.573269823479965	24.65816755393668	25.470159708601848	30.29840291398151
29	17.90725693471561	31.0	23.623984309330346	27.46875875595405
30	19.52479686186607	29.430092462874757	22.258896049313535	28.786214625945643
31	20.00056038105912	29.022975623423932	20.992995236760997	29.983468758755954
32	17.178201176800222	26.047912580554776	22.341552255533763	34.432333987111235
33	19.080975063042867	30.11571868870832	22.183524796861867	28.619781451386945
34	13.811711964135611	23.299243485570187	28.546931913701318	34.34211263659288
35	15.287755673858225	28.242084617539927	26.982908377696834	29.487251330905018
36	15.506024096385543	25.27850938638274	30.266461193611654	28.949005323620064
37	17.400672457270943	28.23676099747828	22.99915942841132	31.363407116839454
38	18.955169515270384	28.97226113757355	26.478565424488654	25.59400392266741
39	17.55701877276548	21.770804146819838	30.83720930232558	29.8349677780891
40	19.396469599327542	29.30989072569347	27.644718408517793	23.648921266461194
41	16.300084057158866	21.160549173437936	37.17736060521155	25.36200616419165
42	21.873353880638835	23.343513589240683	27.66797422247128	27.1151583076492
43	18.230596805827965	22.869151022695434	29.52815914822079	29.372093023255815
44	18.69319137013169	21.55365648641076	27.05463715326422	32.69851499019333
45	25.425329223872232	24.154104791258053	24.85598206780611	25.564583917063604
46	15.77556738582236	21.591202017371813	36.67862146259456	25.954609134211264
47	20.069767441860463	26.98094704398991	25.07312972821519	27.876155785934436
48	17.325021014289717	20.180442701036704	37.8932474082376	24.601288876435977
49	21.03194172036985	20.36368730736901	31.31101148781171	27.293359484449425
50	20.148781171196415	19.145979265900813	27.93723732137854	32.768002241524236
51	16.99915942841132	18.349677780891007	36.7282151863267	27.922947604370975
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	2233.0
1	1741.0
2	1249.0
3	858.5
4	468.0
5	453.5
6	439.0
7	507.5
8	576.0
9	630.0
10	684.0
11	754.0
12	824.0
13	862.0
14	900.0
15	1009.5
16	1119.0
17	1118.5
18	1118.0
19	1250.5
20	1383.0
21	1355.5
22	1328.0
23	1839.5
24	2351.0
25	2159.5
26	2390.5
27	2813.0
28	3522.0
29	4231.0
30	4664.0
31	5097.0
32	5817.5
33	6538.0
34	6814.5
35	7091.0
36	7739.0
37	8387.0
38	9348.5
39	10310.0
40	11081.5
41	11853.0
42	14657.5
43	17462.0
44	32933.0
45	48404.0
46	46822.0
47	45240.0
48	45033.0
49	44826.0
50	40753.0
51	36680.0
52	31385.5
53	26091.0
54	22231.0
55	18371.0
56	15890.5
57	13410.0
58	11669.5
59	9929.0
60	8921.0
61	7913.0
62	6734.0
63	5555.0
64	4662.0
65	3769.0
66	3180.5
67	2592.0
68	1983.0
69	1374.0
70	1140.5
71	907.0
72	728.0
73	549.0
74	427.0
75	283.5
76	262.0
77	219.5
78	177.0
79	111.0
80	45.0
81	36.0
82	27.0
83	29.5
84	32.0
85	20.0
86	8.0
87	7.5
88	7.0
89	5.0
90	3.0
91	2.0
92	1.0
93	0.5
94	0.0
95	0.0
96	0.0
97	0.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	356900.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	29.134855174002166
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.48243553511448	21.70034972322513
2	11.20136082241001	6.527000506253149
3	3.9584420994560277	3.4598591184697334
4	2.032203898880301	2.3683186511168044
5	1.1993922997396813	1.7472060474864504
6	0.7812685617462043	1.3657287839085979
7	0.619623228203735	1.263684311131446
8	0.4929044015476702	1.1488558682975663
9	0.3991128325783372	1.0465285117730063
>10	4.122835490549133	26.36990172465979
>50	0.5757202510952879	11.142040030433135
>100	0.11574271945763155	6.270159189532778
>500	0.005986692385739563	1.3381029602401304
>1k	0.00997782064289927	5.809105247551277
>5k	0.0019955641285798542	5.446599405400627
>10k+	9.977820642899271E-4	2.9965599205203706
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AATGATACCTGTCTCTTATACACATCTGACGCCCACCATTTCGTATGCCGT	10308	2.8882039787055196	No Hit
AATGATACGGCTGTCTCTTATACACATCTGACGCCCACCATTTCGTATGCC	9987	2.7982628187167276	No Hit
AATCTGTCTCTTATACACATCTGACGCCCACCATTTCGTATGCCGTCTTCT	8749	2.4513869431213227	RNA PCR Primer, Index 14 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCCCACCATTTCGTATGCCGTCTTCTGCT	4888	1.3695713084897732	Illumina PCR Primer Index 11 (95% over 23bp)
AATGACTGTCTCTTATACACATCTGACGCCCACCATTTCGTATGCCGTCTT	2429	0.680582796301485	No Hit
CACCCAGGGAATGATACCTGTCTCTTATACACATCTGACGCCCACCATTTC	2008	0.5626225833566825	No Hit
AATGATCTGTCTCTTATACACATCTGACGCCCACCATTTCGTATGCCGTCT	1893	0.530400672457271	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1882	0.5273185766321098	No Hit
AACTGTCTCTTATACACATCTGACGCCCACCATTTCGTATGCCGTCTTCTG	1629	0.45643037265340436	Illumina PCR Primer Index 11 (95% over 21bp)
AATGATACGGCGACTGTCTCTTATACACATCTGACGCCCACCATTTCGTAT	1531	0.4289717007565144	No Hit
AATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCCCACCATTTC	1437	0.40263379097786495	No Hit
CACCCAGGGAATGATACGGCTGTCTCTTATACACATCTGACGCCCACCATT	1245	0.3488372093023256	No Hit
AAACTGTCTCTTATACACATCTGACGCCCACCATTTCGTATGCCGTCTTCT	1041	0.29167834127206504	RNA PCR Primer, Index 14 (95% over 21bp)
CACCCAGGGAATGATCTGTCTCTTATACACATCTGACGCCCACCATTTCGT	882	0.24712804707200897	No Hit
CACCTGTCTCTTATACACATCTGACGCCCACCATTTCGTATGCCGTCTTCT	836	0.23423928271224434	RNA PCR Primer, Index 14 (95% over 21bp)
AAAAACTGTCTCTTATACACATCTGACGCCCACCATTTCGTATGCCGTCTT	795	0.2227514710002802	No Hit
CACCCAGGGAACTGTCTCTTATACACATCTGACGCCCACCATTTCGTATGC	774	0.21686746987951808	No Hit
CCTGTCTCTTATACACATCTGACGCCCACCATTTCGTATGCCGTCTTCTGC	682	0.19108994115998879	Illumina PCR Primer Index 11 (95% over 22bp)
AATGCTGTCTCTTATACACATCTGACGCCCACCATTTCGTATGCCGTCTTC	634	0.17764079574110395	No Hit
AATGATACGGCTGTCTCTTATACACATCTGACGCCCACCCTTTCGTATGCC	488	0.13673297842532922	No Hit
AAAAAACTGTCTCTTATACACATCTGACGCCCACCATTTCGTATGCCGTCT	444	0.12440459512468478	No Hit
AATGATACGGCCTGTCTCTTATACACATCTGACGCCCACCATTTCGTATGC	442	0.12384421406556458	No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	429	0.12020173718128328	No Hit
AATGATACGGCGCTGTCTCTTATACACATCTGACGCCCACCATTTCGTATG	418	0.11711964135612217	No Hit
ACCTGTCTCTTATACACATCTGACGCCCACCATTTCGTATGCCGTCTTCTG	399	0.11179602129448026	RNA PCR Primer, Index 14 (95% over 22bp)
TACCTGTCTCTTATACACATCTGACGCCCACCATTTCGTATGCCGTCTTCT	382	0.10703278229195853	No Hit
CACCCAGGGCTGTCTCTTATACACATCTGACGCCCACCATTTCGTATGCCG	380	0.10647240123283833	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	1.622303166152984	0.0
2	0.0	0.0	0.0	2.0966657326982348	0.0
3	0.0	0.0	0.0	3.086298683104511	0.0
4	2.801905295601009E-4	0.0	0.0	8.212384421406556	0.0
5	2.801905295601009E-4	0.0	0.0	8.745586999159428	0.0
6	2.801905295601009E-4	0.0	0.0	11.30568786775007	0.0
7	2.801905295601009E-4	0.0	0.0	13.107312972821518	0.0
8	2.801905295601009E-4	0.0	0.0	14.156066124964976	0.0
9	2.801905295601009E-4	0.0	0.0	18.596805827963014	0.0
10	2.801905295601009E-4	0.0	0.0	20.64808069487251	0.0
11	2.801905295601009E-4	0.0	0.0	25.53068086298683	0.0
12	2.801905295601009E-4	0.0	0.0	26.97170075651443	0.0
13	2.801905295601009E-4	0.0	0.0	27.656486410759317	0.0
14	2.801905295601009E-4	0.0	0.0	28.60633230596806	0.0
15	2.801905295601009E-4	0.0	0.0	29.109554497058	0.0
16	2.801905295601009E-4	0.0	0.0	30.211824040347437	0.0
17	2.801905295601009E-4	0.0	0.0	31.272065004202858	0.0
18	2.801905295601009E-4	0.0	0.0	33.882600168114315	0.0
19	2.801905295601009E-4	0.0	0.0	34.52031381339311	0.0
20	2.801905295601009E-4	0.0	0.0	35.61529840291398	0.0
21	2.801905295601009E-4	0.0	0.0	36.29532081815635	0.0
22	2.801905295601009E-4	0.0	0.0	37.020173718128326	0.0
23	2.801905295601009E-4	0.0	0.0	37.74894928551415	0.0
24	2.801905295601009E-4	0.0	0.0	38.19641356122163	0.0
25	2.801905295601009E-4	0.0	0.0	38.62230316615298	0.0
26	2.801905295601009E-4	0.0	0.0	38.96581675539367	0.0
27	2.801905295601009E-4	0.0	0.0	39.5463715326422	0.0
28	2.801905295601009E-4	0.0	0.0	40.02465676660129	0.0
29	2.801905295601009E-4	0.0	0.0	40.435976463995516	0.0
30	2.801905295601009E-4	0.0	0.0	41.229476043709724	0.0
31	2.801905295601009E-4	0.0	0.0	41.61978145138694	0.0
32	2.801905295601009E-4	0.0	0.0	42.00728495376856	0.0
33	2.801905295601009E-4	0.0	0.0	42.38358083496778	0.0
34	5.603810591202018E-4	0.0	0.0	42.74978985710283	0.0
35	5.603810591202018E-4	0.0	0.0	43.102829924348555	0.0
36	5.603810591202018E-4	0.0	0.0	43.47856542448865	0.0
37	5.603810591202018E-4	0.0	0.0	43.84001120762118	0.0
38	5.603810591202018E-4	0.0	0.0	44.231997758475764	0.0
39	5.603810591202018E-4	0.0	0.0	44.61221630708882	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTTAACG	25	3.8853825E-5	45.000004	1
CGGGTAG	25	3.8853825E-5	45.000004	6
TACCCTT	25	3.8853825E-5	45.000004	35
GTACCAC	25	3.8853825E-5	45.000004	23
GTTCTCG	25	3.8853825E-5	45.000004	24
CTCAACG	25	3.8853825E-5	45.000004	1
GCGCGTA	25	3.8853825E-5	45.000004	11
GTCCTTC	25	3.8853825E-5	45.000004	30
TTTAACG	25	3.8853825E-5	45.000004	1
CCCAGCG	25	3.8853825E-5	45.000004	1
TACGGGT	25	3.8853825E-5	45.000004	4
CGCGACG	25	3.8853825E-5	45.000004	1
TTCCCGG	25	3.8853825E-5	45.000004	2
CTAGTCG	25	3.8853825E-5	45.000004	1
TGCGAGG	25	3.8853825E-5	45.000004	2
AGCGCGG	50	2.1827873E-11	45.000004	2
CGTAATA	25	3.8853825E-5	45.000004	40
TTTGCGC	25	3.8853825E-5	45.000004	13
ACGTAAT	25	3.8853825E-5	45.000004	39
ATACGTA	25	3.8853825E-5	45.000004	37
>>END_MODULE