##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3548439_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	526519
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.82053449163278	31.0	31.0	34.0	30.0	34.0
2	32.01215720610272	33.0	31.0	34.0	30.0	34.0
3	32.12350361525415	34.0	31.0	34.0	30.0	34.0
4	35.78846537351929	37.0	35.0	37.0	35.0	37.0
5	35.56060844907781	37.0	35.0	37.0	33.0	37.0
6	35.58743369185158	37.0	35.0	37.0	33.0	37.0
7	35.60531718703409	37.0	35.0	37.0	33.0	37.0
8	35.69808117085993	37.0	35.0	37.0	35.0	37.0
9	37.44667523869034	39.0	37.0	39.0	35.0	39.0
10	36.973417863362954	39.0	37.0	39.0	33.0	39.0
11	36.96738769161227	39.0	37.0	39.0	33.0	39.0
12	36.83125965064888	39.0	37.0	39.0	33.0	39.0
13	36.84847650322211	39.0	37.0	39.0	33.0	39.0
14	37.975567833259575	40.0	37.0	41.0	33.0	41.0
15	38.02811294559171	40.0	37.0	41.0	33.0	41.0
16	37.88330905437411	40.0	37.0	41.0	33.0	41.0
17	37.87393237471012	40.0	37.0	41.0	33.0	41.0
18	37.73617476292404	40.0	37.0	41.0	33.0	41.0
19	37.78062709987674	40.0	37.0	41.0	33.0	41.0
20	37.78772465950896	40.0	37.0	41.0	33.0	41.0
21	37.80602789263065	40.0	37.0	41.0	33.0	41.0
22	37.761115933138214	40.0	36.0	41.0	33.0	41.0
23	37.62547220518158	39.0	36.0	41.0	33.0	41.0
24	37.51398714956155	39.0	36.0	41.0	32.0	41.0
25	37.59194634951445	39.0	36.0	41.0	32.0	41.0
26	37.50227437186502	39.0	36.0	41.0	32.0	41.0
27	37.445374241005545	39.0	36.0	41.0	32.0	41.0
28	37.41248463968062	39.0	36.0	41.0	32.0	41.0
29	37.17866401782272	39.0	36.0	41.0	31.0	41.0
30	37.24467113247575	39.0	36.0	41.0	32.0	41.0
31	37.09902776537979	39.0	35.0	41.0	31.0	41.0
32	37.0398637086221	39.0	35.0	41.0	31.0	41.0
33	36.942247098395306	39.0	35.0	41.0	31.0	41.0
34	36.847785170145805	39.0	35.0	41.0	31.0	41.0
35	36.80744664485042	39.0	35.0	41.0	31.0	41.0
36	36.57023393267859	39.0	35.0	41.0	30.0	41.0
37	36.65886701144688	39.0	35.0	41.0	30.0	41.0
38	36.55007701526441	39.0	35.0	40.0	30.0	41.0
39	36.43767841236499	39.0	35.0	40.0	30.0	41.0
40	36.38979030196441	39.0	35.0	40.0	30.0	41.0
41	36.24910212167082	39.0	35.0	40.0	30.0	41.0
42	36.23568380248386	38.0	35.0	40.0	30.0	41.0
43	36.07362127482579	38.0	35.0	40.0	29.0	41.0
44	36.081780524539475	38.0	35.0	40.0	30.0	41.0
45	35.91096997449285	38.0	35.0	40.0	29.0	41.0
46	35.71422493775153	38.0	34.0	40.0	28.0	41.0
47	35.653256577635375	38.0	34.0	40.0	28.0	41.0
48	35.61550675284273	38.0	34.0	40.0	28.0	41.0
49	35.42964831278643	38.0	34.0	40.0	28.0	41.0
50	35.331406843817604	38.0	34.0	40.0	27.0	41.0
51	34.08771383368881	36.0	32.0	40.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	2.0
9	3.0
10	12.0
11	7.0
12	9.0
13	16.0
14	28.0
15	34.0
16	65.0
17	111.0
18	187.0
19	364.0
20	694.0
21	1065.0
22	1440.0
23	1850.0
24	2428.0
25	3008.0
26	3556.0
27	4506.0
28	5947.0
29	7640.0
30	9867.0
31	13047.0
32	16832.0
33	22769.0
34	34479.0
35	41212.0
36	48951.0
37	68461.0
38	107129.0
39	130772.0
40	28.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	21.935770598971736	34.51081537418403	18.151291786241334	25.402122240602903
2	25.297092792472824	33.504014100155935	18.628007726216907	22.570885381154337
3	20.813114056662723	29.250226487553157	24.352397539310072	25.58426191647405
4	24.468632660929615	29.653440806504612	18.088995838706676	27.788930693859097
5	18.177121813267895	35.28647589165823	17.728894873689267	28.807507421384603
6	20.583872566801958	35.28495647830373	22.58133134796655	21.549839606927765
7	80.73801705161637	8.443000157639135	6.265680820635152	4.553301970109341
8	81.87890655417944	4.662129951625677	4.932965382065984	8.525998112128907
9	77.49027100636444	7.8658130095969945	8.679268934264481	5.964647049774082
10	34.45573664008326	33.66032374900051	14.959004328428794	16.924935282487432
11	26.298196266421535	28.043622357407806	26.140747057561075	19.517434318609585
12	25.80609626623161	25.378761260277404	31.26819734900355	17.546945124487433
13	26.638734784499707	26.98857970937421	25.970762688525962	20.401922817600123
14	21.52761818661815	27.05049580357024	30.638590440231027	20.783295569580584
15	19.461785804500884	29.663886773316822	27.41515500865116	23.459172413531135
16	22.251998503377845	28.91044767615224	27.862622241552536	20.97493157891738
17	21.552878433636774	27.74163895320017	27.65313312530032	23.052349487862735
18	23.352623552046555	26.792575386643218	26.65259943135955	23.202201629950675
19	23.136297075699073	29.725992794182165	26.97205608914398	20.16565404097478
20	24.70680070424809	29.459525677136057	25.910935787692374	19.92273783092348
21	24.6615981569516	27.801465854033758	26.966548215733905	20.570387773280736
22	23.86751475255404	27.943531002679865	26.17227488466703	22.016679360099065
23	22.68275218937968	28.585862998296356	25.453972221325344	23.277412590998615
24	19.895768243881037	31.178741887757138	25.756335478871605	23.169154389490217
25	23.22917121699312	28.04476191742368	24.788849025391297	23.937217840191902
26	23.9474738803348	28.26146824710979	24.126194876158316	23.66486299639709
27	20.629454967437074	26.68621645182795	27.717138412858795	24.967190167876183
28	24.3425213525058	27.179076158695125	26.11548681054245	22.362915678256627
29	22.325500124401966	29.613746132618196	27.391983954994974	20.668769787984857
30	24.218309310775112	26.258501592535122	25.244103251734508	24.27908584495526
31	24.792077778769617	30.03367399846919	23.839975385503656	21.334272837257533
32	23.43581143320564	27.81514057422429	25.146670870376948	23.602377122193122
33	24.633109156554656	27.73347210641971	25.61825879028107	22.01515994674456
34	23.218155470172967	26.947175695463983	27.729483646364137	22.105185187998913
35	24.141578936372664	27.56253810403803	26.74167503926734	21.554207920321964
36	23.058996921288692	29.196097386798957	28.00316797684414	19.74173771506821
37	22.715039723162885	30.10622598614675	22.658821429046245	24.51991286164412
38	23.905500086416634	31.227173188431944	24.669575077062746	20.197751648088673
39	24.26996936482824	27.02162694983467	25.335078126335418	23.37332555900167
40	26.283951766223062	27.700804719297878	24.431596960413586	21.583646554065474
41	24.04870479507862	24.57594122909145	28.68614427969361	22.689209696136324
42	26.630567937719245	26.783458906516195	24.384305219754655	22.201667936009905
43	23.01683320070121	25.524435015640464	27.436236868944903	24.02249491471343
44	23.301153424662736	24.31023381872259	26.262490052590692	26.126122704023974
45	26.024511935941536	26.75326056609543	25.249041345136643	21.973186152826393
46	20.915864384761043	26.422598234821535	29.749733627846286	22.91180375257113
47	23.76172559774671	28.258049567062155	24.673943390456945	23.30628144473419
48	22.866601205274645	25.601735170050844	29.556958058493617	21.974705566180898
49	24.110241035936024	24.57100313568931	27.48770699632872	23.831048832045948
50	21.705959329103035	26.688875425198333	26.190697771590386	25.41446747410825
51	22.261874690182122	24.055352228504574	29.96852915089484	23.714243930418466
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1623.0
1	1286.5
2	950.0
3	678.5
4	407.0
5	389.5
6	372.0
7	375.0
8	378.0
9	407.0
10	436.0
11	486.0
12	536.0
13	649.5
14	763.0
15	756.5
16	750.0
17	757.0
18	764.0
19	783.0
20	802.0
21	1454.5
22	2107.0
23	2211.5
24	2316.0
25	2980.5
26	4056.5
27	4468.0
28	6007.5
29	7547.0
30	8607.5
31	9668.0
32	10398.0
33	11128.0
34	12662.5
35	14197.0
36	15852.0
37	17507.0
38	19320.5
39	21134.0
40	21804.5
41	22475.0
42	23750.5
43	25026.0
44	35023.0
45	45020.0
46	47172.5
47	49325.0
48	48241.5
49	47158.0
50	45782.5
51	44407.0
52	40955.0
53	37503.0
54	34514.0
55	31525.0
56	29130.0
57	26735.0
58	24794.0
59	22853.0
60	21476.5
61	20100.0
62	18522.0
63	16944.0
64	13507.0
65	10070.0
66	8948.0
67	7826.0
68	6806.0
69	5786.0
70	5228.5
71	4671.0
72	3793.0
73	2915.0
74	2463.0
75	1481.0
76	951.0
77	1014.5
78	1078.0
79	695.5
80	313.0
81	246.0
82	179.0
83	110.0
84	41.0
85	39.5
86	38.0
87	37.0
88	36.0
89	30.0
90	24.0
91	15.5
92	7.0
93	5.5
94	4.0
95	2.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	526519.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	26.167997428987988
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.01874847372517	20.154264120443127
2	9.076431087050508	4.750240507006487
3	3.113398252526735	2.4441419240260593
4	1.4585808617901224	1.5267256096518003
5	0.9040400261930207	1.1828458540560602
6	0.6340917544157096	0.9955746839575664
7	0.5269307885255847	0.965210646357448
8	0.4129458276670668	0.8644772285362496
9	0.3501635510517464	0.8246771013282664
>10	5.010719981369466	31.961573350466928
>50	1.3487853674908863	24.01129785329058
>100	0.13641041845364832	5.218851427075432
>500	0.0036473373918087786	0.7204076549298712
>1k	0.0036473373918087786	2.1533965965193103
>5k	0.0014589349567235115	2.2263154423548195
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AATGATACCTGTCTCTTATACACATCTGACGCGTCAAAGGTCGTATGCCGT	5897	1.1199975689386328	No Hit
AATGATACGGCTGTCTCTTATACACATCTGACGCGTCAAAGGTCGTATGCC	5766	1.0951171752586326	No Hit
AATCTGTCTCTTATACACATCTGACGCGTCAAAGGTCGTATGCCGTCTTCT	4882	0.9272219995859597	No Hit
CTGTCTCTTATACACATCTGACGCGTCAAAGGTCGTATGCCGTCTTCTGCT	2879	0.5467988809520644	Illumina Single End Adapter 1 (95% over 22bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1289	0.24481547674442897	No Hit
AATGACTGTCTCTTATACACATCTGACGCGTCAAAGGTCGTATGCCGTCTT	1219	0.2315206098925205	No Hit
AATGATCTGTCTCTTATACACATCTGACGCGTCAAAGGTCGTATGCCGTCT	1012	0.192205789344734	No Hit
AATGATACGGCGACTGTCTCTTATACACATCTGACGCGTCAAAGGTCGTAT	896	0.17017429570442852	No Hit
AATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGTCAAAGGTC	847	0.1608678889080926	No Hit
AACTGTCTCTTATACACATCTGACGCGTCAAAGGTCGTATGCCGTCTTCTG	800	0.1519413354503826	No Hit
AATGCTGTCTCTTATACACATCTGACGCGTCAAAGGTCGTATGCCGTCTTC	664	0.12611130842381757	No Hit
AAACTGTCTCTTATACACATCTGACGCGTCAAAGGTCGTATGCCGTCTTCT	567	0.10768842150045868	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	3.798533386259565E-4	0.0	0.0	0.637393902214355	0.0
2	3.798533386259565E-4	0.0	0.0	0.8523908918766464	0.0
3	3.798533386259565E-4	0.0	0.0	1.2537059441349694	0.0
4	3.798533386259565E-4	0.0	0.0	3.2528740653233785	0.0
5	3.798533386259565E-4	0.0	0.0	3.557896296240022	0.0
6	3.798533386259565E-4	0.0	0.0	4.688719685329494	0.0
7	3.798533386259565E-4	0.0	0.0	5.515280550179575	0.0
8	3.798533386259565E-4	0.0	0.0	5.9946554635255325	0.0
9	3.798533386259565E-4	0.0	0.0	7.8242190690174525	0.0
10	3.798533386259565E-4	0.0	0.0	8.871664650278527	0.0
11	3.798533386259565E-4	0.0	0.0	10.988587306440984	0.0
12	3.798533386259565E-4	0.0	0.0	11.565394601144497	0.0
13	3.798533386259565E-4	0.0	0.0	11.874975072124652	0.0
14	3.798533386259565E-4	0.0	0.0	12.325101278396412	0.0
15	3.798533386259565E-4	0.0	0.0	12.595936708836717	0.0
16	3.798533386259565E-4	0.0	0.0	13.063536168685271	0.0
17	3.798533386259565E-4	0.0	0.0	13.634075883301458	0.0
18	3.798533386259565E-4	0.0	0.0	14.564336709596425	0.0
19	3.798533386259565E-4	0.0	0.0	14.891960214161312	0.0
20	3.798533386259565E-4	0.0	0.0	15.257379125919483	0.0
21	3.798533386259565E-4	0.0	0.0	15.68889251859857	0.0
22	5.697800079389348E-4	0.0	0.0	16.1035024377088	0.0
23	5.697800079389348E-4	0.0	0.0	16.515073530110026	0.0
24	5.697800079389348E-4	0.0	0.0	16.819905834357353	0.0
25	5.697800079389348E-4	0.0	0.0	17.090931191466975	0.0
26	5.697800079389348E-4	0.0	0.0	17.360057281883464	0.0
27	5.697800079389348E-4	0.0	0.0	17.648176039231252	0.0
28	5.697800079389348E-4	0.0	0.0	17.92793802312927	0.0
29	5.697800079389348E-4	0.0	0.0	18.22631282061996	0.0
30	5.697800079389348E-4	0.0	0.0	18.652888119896907	0.0
31	5.697800079389348E-4	0.0	0.0	18.92543289036103	0.0
32	5.697800079389348E-4	0.0	0.0	19.205574727597675	0.0
33	5.697800079389348E-4	0.0	0.0	19.4986315783476	0.0
34	5.697800079389348E-4	0.0	0.0	19.796056742491725	0.0
35	5.697800079389348E-4	0.0	0.0	20.11019545353539	0.0
36	5.697800079389348E-4	0.0	0.0	20.373053963864553	0.0
37	5.697800079389348E-4	0.0	0.0	20.660223087865774	0.0
38	5.697800079389348E-4	0.0	0.0	20.95954751870303	0.0
39	5.697800079389348E-4	0.0	0.0	21.305403983521963	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGGGTTA	30	2.1632513E-6	45.000004	6
CAACGCT	20	7.0297986E-4	45.000004	24
CAACGAA	20	7.0297986E-4	45.000004	19
GCGGTCA	25	3.8879152E-5	45.000004	32
GACCGTA	20	7.0297986E-4	45.000004	27
GAATCGC	30	2.1632513E-6	45.000004	22
ATTGTCG	25	3.8879152E-5	45.000004	10
ATTCGAA	30	2.1632513E-6	45.000004	10
CACGATA	25	3.8879152E-5	45.000004	22
ATCGTAG	20	7.0297986E-4	45.000004	1
AATGCGG	80	0.0	45.000004	2
TCTACCG	25	3.8879152E-5	45.000004	1
TACGATC	20	7.0297986E-4	45.000004	36
CGTAGCG	25	3.8879152E-5	45.000004	1
ACTACTA	25	3.8879152E-5	45.000004	41
AAACCCG	30	2.1632513E-6	45.000004	1
AGCGATC	20	7.0297986E-4	45.000004	42
CGAATCT	20	7.0297986E-4	45.000004	22
CGTACTC	20	7.0297986E-4	45.000004	45
GGACGAC	20	7.0297986E-4	45.000004	8
>>END_MODULE