##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3548438_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	228586
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.64416018478822	31.0	31.0	34.0	30.0	34.0
2	31.84436054701513	33.0	31.0	34.0	30.0	34.0
3	32.00980375001094	33.0	31.0	34.0	30.0	34.0
4	35.69531379874533	37.0	35.0	37.0	33.0	37.0
5	35.4484307875373	37.0	35.0	37.0	33.0	37.0
6	34.89263121975974	37.0	35.0	37.0	32.0	37.0
7	35.16145345734209	37.0	35.0	37.0	32.0	37.0
8	35.52644081439808	37.0	35.0	37.0	33.0	37.0
9	37.18970978100146	39.0	37.0	39.0	34.0	39.0
10	36.85705161295967	39.0	37.0	39.0	32.0	39.0
11	36.8574015906486	39.0	37.0	39.0	32.0	39.0
12	36.71687242438295	39.0	35.0	39.0	32.0	39.0
13	36.71849544591532	39.0	35.0	39.0	32.0	39.0
14	37.81696604341473	40.0	37.0	41.0	33.0	41.0
15	37.864641754088176	40.0	37.0	41.0	33.0	41.0
16	37.728023588496235	40.0	36.0	41.0	33.0	41.0
17	37.717498009501895	39.0	36.0	41.0	33.0	41.0
18	37.62665255089988	39.0	36.0	41.0	33.0	41.0
19	37.54552334788657	39.0	36.0	41.0	32.0	41.0
20	37.4897937756468	39.0	36.0	41.0	32.0	41.0
21	37.46507660136666	39.0	36.0	41.0	32.0	41.0
22	37.45297612277217	39.0	36.0	41.0	32.0	41.0
23	37.293491289930266	39.0	35.0	41.0	32.0	41.0
24	37.17291085193319	39.0	35.0	41.0	32.0	41.0
25	37.22738487921395	39.0	35.0	41.0	32.0	41.0
26	37.151050370538876	39.0	35.0	41.0	32.0	41.0
27	36.98277672298391	39.0	35.0	40.0	31.0	41.0
28	36.94557846937258	39.0	35.0	40.0	31.0	41.0
29	36.67039101257295	39.0	35.0	40.0	30.0	41.0
30	36.61426334071203	39.0	35.0	40.0	30.0	41.0
31	36.57785253690077	39.0	35.0	40.0	30.0	41.0
32	36.499759390338866	39.0	35.0	40.0	30.0	41.0
33	36.23665491324928	39.0	35.0	40.0	30.0	41.0
34	36.2516514572196	39.0	35.0	40.0	30.0	41.0
35	36.13182784597482	38.0	35.0	40.0	30.0	41.0
36	36.07678947967067	38.0	35.0	40.0	30.0	41.0
37	35.91102692203372	38.0	35.0	40.0	29.0	41.0
38	35.76109210537828	38.0	35.0	40.0	29.0	41.0
39	35.80657608077485	38.0	35.0	40.0	29.0	41.0
40	35.63457954555397	38.0	35.0	40.0	28.0	41.0
41	35.655840690156005	38.0	35.0	40.0	28.0	41.0
42	35.53253480090644	38.0	35.0	40.0	28.0	41.0
43	35.429553865941045	38.0	34.0	40.0	27.0	41.0
44	35.22878478996964	38.0	34.0	40.0	26.0	41.0
45	35.01256419903231	38.0	34.0	40.0	26.0	41.0
46	34.77680172888979	37.0	34.0	40.0	25.0	41.0
47	34.64042417295897	37.0	33.0	40.0	25.0	41.0
48	34.573687802402596	37.0	33.0	40.0	25.0	41.0
49	34.46692273367573	37.0	33.0	40.0	24.0	41.0
50	34.34164821992598	37.0	33.0	40.0	24.0	41.0
51	33.0331253882565	35.0	31.0	39.0	20.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	0.0
10	1.0
11	2.0
12	2.0
13	3.0
14	6.0
15	12.0
16	41.0
17	72.0
18	148.0
19	300.0
20	495.0
21	734.0
22	1115.0
23	1361.0
24	1639.0
25	1770.0
26	2129.0
27	2520.0
28	3110.0
29	3845.0
30	4886.0
31	6211.0
32	8034.0
33	10495.0
34	15681.0
35	20300.0
36	22450.0
37	31401.0
38	44845.0
39	44969.0
40	8.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	20.24795919260147	35.519235648727395	18.490633722100217	25.74217143657092
2	22.443631718477945	34.92558599389289	19.263646942507414	23.36713534512175
3	20.072095403918	31.953837942831143	23.00403349286483	24.970033160386027
4	21.86748094808956	32.72991346801641	19.25096025128398	26.151645332610045
5	17.99847759705319	35.21125528247574	18.521694241992073	28.268572878479
6	18.424575433316125	37.541231746476164	21.101029809349654	22.933163010858056
7	76.03221544626531	11.058420025723361	7.306659200475969	5.60270532753537
8	77.85778656610641	7.121608497458287	6.0493643530224945	8.971240583412808
9	72.21002161112229	10.589012450456284	10.939427611489768	6.261538326931658
10	34.5668588627475	33.02126989404425	13.900676331883844	18.511194911324402
11	25.1318978415126	30.149265484325372	25.063652192172746	19.655184481989274
12	26.087336932270567	24.41969324455566	31.29894219243523	18.19402763073854
13	25.541809209662887	26.598741830208322	25.800355227354256	22.059093732774535
14	18.466135283875655	29.173702676454376	30.637046888260873	21.723115151409097
15	16.686061263594446	30.27307009178165	29.13651754700638	23.90435109761753
16	19.141154751384597	29.328567803802507	28.294821205148175	23.23545623966472
17	17.148469285083078	28.91603160298531	28.67629688607351	25.2592022258581
18	21.979473808544707	28.60980112517827	24.65680312880054	24.753921937476488
19	20.404574208394216	31.754350660145413	27.31007148294296	20.531003648517405
20	23.085403305539273	29.741104004619707	24.98315732372061	22.190335366120408
21	22.108528081334818	30.141828458435775	25.72161024734673	22.02803321288268
22	21.553375972281767	28.66973480440622	25.313448767641063	24.463440455670952
23	21.270768988476984	28.90859457709571	24.24426692798334	25.576369506443964
24	18.769740929015775	31.198761078981214	25.005905873500566	25.025592118502445
25	21.535002143613344	28.973777921657494	24.219331017647626	25.271888917081537
26	23.94109875495437	29.75729047273236	23.522437944581032	22.779172827732232
27	20.560751752075806	27.048463160473517	27.407627763730062	24.98315732372061
28	23.755610579825536	27.28819787738532	26.37256874874227	22.583622794046878
29	25.067589441173126	29.241073381571926	23.97478410751315	21.716553069741803
30	23.866728496058375	25.589493669778552	26.80041647344982	23.743361360713255
31	25.72117277523558	28.10364589257435	23.287077948780766	22.88810338340931
32	26.175706298723455	24.77667048725644	24.99190676594367	24.055716448076435
33	22.74898725206268	25.09427523995345	29.65448452661143	22.502252981372436
34	23.74423630493556	24.158959866308525	26.871724427567745	25.22507940118817
35	25.702361474456005	22.832544425292888	30.350502655455713	21.114591444795394
36	24.889100819822737	24.473939786338622	30.0477719545379	20.589187439300744
37	23.50668894857953	25.523435380994464	27.406315347396603	23.563560323029407
38	23.623931474368508	25.980593737149256	29.954152922751177	20.44132186573106
39	25.245203118301205	21.81060957363968	28.24801168925481	24.6961756188043
40	25.05359033361623	23.735924334823654	27.502121739739096	23.708363591821023
41	21.09096795079314	21.62599634273315	31.021147401853135	26.26188830462058
42	26.093024069715554	23.75473563560323	26.686673724550058	23.465566570131156
43	22.77348569028724	22.426570306142985	28.50612023483503	26.293823768734743
44	23.93628656173169	20.716491823646244	28.282571986035894	27.064649628586178
45	26.71817171655307	22.17983603545274	28.454935997830137	22.647056250164052
46	22.00440971888042	22.600684206381843	31.423184272002658	23.971721802735075
47	23.163710813435642	24.21714365709186	28.150892880578866	24.468252648893632
48	22.696928070835483	22.319827111021674	31.513740999011315	23.46950381913153
49	23.406070363014358	21.0253471341202	30.151015372769983	25.41756713009546
50	20.807048550654898	21.93091440420673	28.074772733238255	29.18726431190012
51	21.853919312643818	20.673619556753255	31.327815351771328	26.1446457788316
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	2341.0
1	1824.0
2	1307.0
3	898.0
4	489.0
5	436.5
6	384.0
7	416.0
8	448.0
9	471.0
10	494.0
11	535.5
12	577.0
13	579.0
14	581.0
15	593.5
16	606.0
17	705.5
18	805.0
19	784.5
20	764.0
21	792.0
22	820.0
23	1149.0
24	1478.0
25	1293.5
26	1389.5
27	1670.0
28	2162.0
29	2654.0
30	2575.0
31	2496.0
32	3116.0
33	3736.0
34	3920.0
35	4104.0
36	4592.5
37	5081.0
38	5739.5
39	6398.0
40	6934.5
41	7471.0
42	8905.0
43	10339.0
44	13980.0
45	17621.0
46	19234.5
47	20848.0
48	22561.5
49	24275.0
50	22862.5
51	21450.0
52	19581.5
53	17713.0
54	15828.0
55	13943.0
56	12791.5
57	11640.0
58	11405.0
59	11170.0
60	9939.5
61	8709.0
62	7992.0
63	7275.0
64	6509.5
65	5744.0
66	4882.0
67	4020.0
68	3426.0
69	2832.0
70	2283.0
71	1734.0
72	1458.5
73	1183.0
74	1089.0
75	822.0
76	649.0
77	398.0
78	147.0
79	177.0
80	207.0
81	170.0
82	133.0
83	130.0
84	127.0
85	69.0
86	11.0
87	8.0
88	5.0
89	3.0
90	1.0
91	1.5
92	2.0
93	1.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	228586.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	33.8690908454586
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.94859209506588	26.73917037788841
2	9.179798501679153	6.21822858792752
3	3.1658486179281837	3.2167324333073766
4	1.5448204598295014	2.092866579755541
5	0.8524928958925343	1.443657966804616
6	0.5631619736502196	1.144427042776023
7	0.43528803926633947	1.031996710209724
8	0.37199690002583313	1.0079357440963137
9	0.2777060191165074	0.8465085350808886
>10	3.8349263756135366	30.591549788700974
>50	0.7452854559545338	16.533383496802077
>100	0.07104107465771119	4.212856430402562
>500	0.0025833118057349523	0.6321472006159607
>1k	0.006458279514337381	4.288539105632016
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CTGTCTCTTATACACATCTGACGCGGTTGTCTTCGTATGCCGTCTTCTGCT	2471	1.0809935866588505	No Hit
AATGATACGGCTGTCTCTTATACACATCTGACGCGGTTGTCTTCGTATGCC	2026	0.8863184971958038	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2021	0.8841311366400392	No Hit
AATGATACCTGTCTCTTATACACATCTGACGCGGTTGTCTTCGTATGCCGT	1802	0.788324744297551	No Hit
AATCTGTCTCTTATACACATCTGACGCGGTTGTCTTCGTATGCCGTCTTCT	1483	0.6487711408397714	No Hit
AAACTGTCTCTTATACACATCTGACGCGGTTGTCTTCGTATGCCGTCTTCT	895	0.39153753948185804	No Hit
AAAAACTGTCTCTTATACACATCTGACGCGGTTGTCTTCGTATGCCGTCTT	550	0.24060966113410273	No Hit
AATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGGTTGTCTTC	482	0.21086155757570457	No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	461	0.20167464324149337	No Hit
CCTGTCTCTTATACACATCTGACGCGGTTGTCTTCGTATGCCGTCTTCTGC	414	0.18111345401730639	No Hit
AATGACTGTCTCTTATACACATCTGACGCGGTTGTCTTCGTATGCCGTCTT	414	0.18111345401730639	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	374	0.16361456957118983	No Hit
AAAAAACTGTCTCTTATACACATCTGACGCGGTTGTCTTCGTATGCCGTCT	339	0.14830304568083785	No Hit
AACTGTCTCTTATACACATCTGACGCGGTTGTCTTCGTATGCCGTCTTCTG	337	0.14742810145853202	No Hit
AATGATCTGTCTCTTATACACATCTGACGCGGTTGTCTTCGTATGCCGTCT	314	0.137366242902015	No Hit
GAAAAACTGTCTCTTATACACATCTGACGCGGTTGTCTTCGTATGCCGTCT	262	0.11461769312206348	No Hit
AATGATACGGCGACTGTCTCTTATACACATCTGACGCGGTTGTCTTCGTAT	251	0.1098054998993814	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	4.37472111152914E-4	0.0	0.0	1.2218596064500888	0.0
2	4.37472111152914E-4	0.0	0.0	1.6243339487107697	0.0
3	4.37472111152914E-4	0.0	0.0	2.1759862808745942	0.0
4	4.37472111152914E-4	0.0	0.0	4.736073075341447	0.0
5	4.37472111152914E-4	0.0	0.0	5.097425039153754	0.0
6	4.37472111152914E-4	0.0	0.0	6.857375342321927	0.0
7	4.37472111152914E-4	0.0	0.0	8.212226470562502	0.0
8	4.37472111152914E-4	0.0	0.0	9.197851136990018	0.0
9	4.37472111152914E-4	0.0	0.0	11.178287384179258	0.0
10	4.37472111152914E-4	0.0	0.0	13.237468611376025	0.0
11	4.37472111152914E-4	0.0	0.0	15.839115256402405	0.0
12	4.37472111152914E-4	0.0	0.0	16.834364309275283	0.0
13	4.37472111152914E-4	0.0	0.0	17.262212033982834	0.0
14	4.37472111152914E-4	0.0	0.0	17.790678344255554	0.0
15	4.37472111152914E-4	0.0	0.0	18.205401905628516	0.0
16	4.37472111152914E-4	0.0	0.0	18.96266613003421	0.0
17	4.37472111152914E-4	0.0	0.0	19.8992939200126	0.0
18	4.37472111152914E-4	0.0	0.0	21.475068464385394	0.0
19	4.37472111152914E-4	0.0	0.0	22.058656260663383	0.0
20	4.37472111152914E-4	0.0	0.0	22.657555580831723	0.0
21	4.37472111152914E-4	0.0	0.0	23.19870858232788	0.0
22	4.37472111152914E-4	0.0	0.0	23.7875460439397	0.0
23	4.37472111152914E-4	0.0	0.0	24.313824993656656	0.0
24	4.37472111152914E-4	0.0	0.0	24.67517695746896	0.0
25	4.37472111152914E-4	0.0	0.0	24.977470186275625	0.0
26	4.37472111152914E-4	0.0	0.0	25.289825273638805	0.0
27	4.37472111152914E-4	0.0	0.0	25.699736641789087	0.0
28	4.37472111152914E-4	0.0	0.0	26.06677574304638	0.0
29	4.37472111152914E-4	0.0	0.0	26.417190904079863	0.0
30	4.37472111152914E-4	0.0	0.0	26.993779146579406	0.0
31	4.37472111152914E-4	0.0	0.0	27.331070144278304	0.0
32	4.37472111152914E-4	0.0	0.0	27.655674450753764	0.0
33	4.37472111152914E-4	0.0	0.0	27.988153255229978	0.0
34	4.37472111152914E-4	0.0	0.0	28.279072209146666	0.0
35	4.37472111152914E-4	0.0	0.0	28.592302240732153	0.0
36	4.37472111152914E-4	0.0	0.0	28.8919706368719	0.0
37	4.37472111152914E-4	0.0	0.0	29.191201560900492	0.0
38	4.37472111152914E-4	0.0	0.0	29.550366164157037	0.0
39	4.37472111152914E-4	0.0	0.0	29.870158277409814	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACTATGG	35	1.2062264E-7	45.000004	2
ATTCCGG	35	1.2062264E-7	45.000004	2
CGTACCA	35	1.2062264E-7	45.000004	45
GCGTAGG	70	0.0	45.000004	2
GCATAAG	20	7.021418E-4	45.0	1
TCTGCAA	25	3.8809696E-5	45.0	39
CGTGGTG	20	7.021418E-4	45.0	39
AGGTAAT	25	3.8809696E-5	45.0	6
GCCTTAC	20	7.021418E-4	45.0	17
TCGCAGG	25	3.8809696E-5	45.0	2
TCGCAAT	20	7.021418E-4	45.0	40
TACCCCG	20	7.021418E-4	45.0	25
CAAGTTA	20	7.021418E-4	45.0	32
CTCCGTA	20	7.021418E-4	45.0	17
CTACTCG	20	7.021418E-4	45.0	41
CTCGTAC	20	7.021418E-4	45.0	29
GGTCGTA	20	7.021418E-4	45.0	13
TACTTGC	25	3.8809696E-5	45.0	42
GGGCTAT	20	7.021418E-4	45.0	7
GTCTGCG	20	7.021418E-4	45.0	26
>>END_MODULE