##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3548435_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	391891
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.758700761181043	31.0	31.0	34.0	30.0	34.0
2	31.94279786981584	33.0	31.0	34.0	30.0	34.0
3	32.066444496046095	34.0	31.0	34.0	30.0	34.0
4	35.73657726255515	37.0	35.0	37.0	33.0	37.0
5	35.5312599676951	37.0	35.0	37.0	33.0	37.0
6	35.55423574412273	37.0	35.0	37.0	33.0	37.0
7	35.55199277350079	37.0	35.0	37.0	33.0	37.0
8	35.63589110237285	37.0	35.0	37.0	35.0	37.0
9	37.32296735571881	39.0	37.0	39.0	34.0	39.0
10	36.90167674174707	39.0	37.0	39.0	32.0	39.0
11	36.95549527802373	39.0	37.0	39.0	33.0	39.0
12	36.884506150945036	39.0	37.0	39.0	33.0	39.0
13	36.87900972464282	39.0	37.0	39.0	33.0	39.0
14	38.0027813856404	40.0	37.0	41.0	33.0	41.0
15	38.05909041034369	40.0	37.0	41.0	33.0	41.0
16	37.95296651364793	40.0	37.0	41.0	33.0	41.0
17	37.92344299818062	40.0	37.0	41.0	33.0	41.0
18	37.79282504573976	40.0	37.0	41.0	33.0	41.0
19	37.849698002760974	40.0	37.0	41.0	33.0	41.0
20	37.807941493935814	40.0	37.0	41.0	33.0	41.0
21	37.807566389633855	40.0	37.0	41.0	33.0	41.0
22	37.76965278610634	40.0	37.0	41.0	33.0	41.0
23	37.64380912039317	39.0	36.0	41.0	33.0	41.0
24	37.52399008908089	39.0	36.0	41.0	32.0	41.0
25	37.54780028120064	39.0	36.0	41.0	32.0	41.0
26	37.49150146341712	39.0	36.0	41.0	32.0	41.0
27	37.430632497301545	39.0	36.0	41.0	32.0	41.0
28	37.25751548262144	39.0	36.0	41.0	32.0	41.0
29	37.08423770895479	39.0	36.0	41.0	31.0	41.0
30	37.097034634630546	39.0	36.0	41.0	31.0	41.0
31	37.028780962053226	39.0	36.0	41.0	31.0	41.0
32	36.983620445481016	39.0	36.0	41.0	31.0	41.0
33	36.89060478551434	39.0	35.0	41.0	31.0	41.0
34	36.75522785672547	39.0	35.0	41.0	30.0	41.0
35	36.71569645641262	39.0	35.0	41.0	30.0	41.0
36	36.62456397314559	39.0	35.0	40.0	30.0	41.0
37	36.57294757981173	39.0	35.0	40.0	30.0	41.0
38	36.53020099976779	39.0	35.0	40.0	30.0	41.0
39	36.41947633398062	39.0	35.0	40.0	30.0	41.0
40	36.282450987647074	39.0	35.0	40.0	30.0	41.0
41	36.12687456461108	38.0	35.0	40.0	30.0	41.0
42	36.10396002970214	38.0	35.0	40.0	30.0	41.0
43	36.063806007282636	38.0	35.0	40.0	30.0	41.0
44	36.01557065612632	38.0	35.0	40.0	30.0	41.0
45	35.89445789773177	38.0	35.0	40.0	29.0	41.0
46	35.67090338895254	38.0	35.0	40.0	29.0	41.0
47	35.53610315113131	38.0	34.0	40.0	28.0	41.0
48	35.45272792689804	38.0	34.0	40.0	28.0	41.0
49	35.262108596523014	38.0	34.0	40.0	27.0	41.0
50	35.1781566813221	37.0	34.0	40.0	27.0	41.0
51	33.78492999328895	36.0	32.0	39.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	2.0
9	0.0
10	4.0
11	4.0
12	3.0
13	6.0
14	7.0
15	23.0
16	31.0
17	75.0
18	139.0
19	317.0
20	564.0
21	900.0
22	1241.0
23	1627.0
24	2021.0
25	2322.0
26	2844.0
27	3407.0
28	4331.0
29	5455.0
30	7384.0
31	9350.0
32	12581.0
33	16866.0
34	24462.0
35	31591.0
36	37866.0
37	53637.0
38	81172.0
39	91633.0
40	26.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	20.256652997899927	35.04596941496488	19.65903784470682	25.038339742428377
2	22.937755651443897	34.12556042368924	19.29414046252657	23.642543462340292
3	19.942790214626005	31.72846531305899	23.671122837727836	24.65762163458717
4	22.695596479633366	31.064760354282186	18.283655404181264	27.955987761903184
5	18.101972232074733	35.53615673746016	18.597518187455186	27.76435284300992
6	19.802700240628134	35.11333508552123	22.115588263062943	22.968376410787695
7	79.59330528131547	8.709564649354029	6.679663477854812	5.017466591475691
8	80.47365211244963	5.246611940565106	5.77839246116905	8.501343485816209
9	75.56412369766083	9.021130875677166	9.470490519047388	5.944254907614617
10	32.33271496410992	35.157990359564266	14.773750864398519	17.735543811927297
11	23.850764625878114	29.241549308353598	26.810005843461575	20.097680222306714
12	24.388414125356288	23.782633436338166	33.44935198818039	18.379600450125164
13	26.111852530423	25.957728041725886	26.00467987271971	21.9257395551314
14	19.777948460158566	28.413257768103882	30.781007984363	21.02778578737455
15	17.529874378334792	29.943019870321088	29.545970690829847	22.981135060514276
16	19.635561929209906	29.358163366854562	28.123891592305007	22.88238311163053
17	18.93358102125336	27.51606951933063	27.57705586502369	25.973293594392317
18	22.564947906433165	27.31575871862329	25.9005692909508	24.218724083992743
19	22.236540262470943	29.500294724808683	28.18309172703635	20.08007328568403
20	24.026833992104947	28.72916193533406	24.887277329665647	22.356726742895347
21	23.086521507255846	27.70668374624577	26.46475678186026	22.742037964638126
22	20.931330395441588	28.271126410149762	26.60586744783626	24.19167574657239
23	22.32636115654608	27.807477079085768	24.383821011454714	25.482340752913434
24	19.454389103092442	30.024164882582145	25.83192775542179	24.68951825890362
25	20.386280879122	28.549775320178316	25.203181496895823	25.860762303803863
26	22.668803315207544	28.24509876470753	23.69485392621928	25.391243993865643
27	19.917783261161905	27.36449676057883	26.449956748177428	26.267763230081837
28	21.465152300001787	28.927686525079675	26.652564105835552	22.954597069082986
29	21.263820807316318	29.945316427271866	24.508345432786157	24.282517332625652
30	23.328680679066373	29.43088767029608	23.739254027267787	23.501177623369763
31	23.91532339349462	29.025417781985297	24.08271687790738	22.976541946612706
32	24.526207542403373	27.839118530407692	23.515467311063535	24.119206616125403
33	23.93599240605168	27.461972844489924	25.427478559089135	23.174556190369263
34	21.440400519532215	26.68803315207545	27.740621754518475	24.130944573873858
35	24.03678573889168	27.9432291121766	25.498671824563463	22.521313324368254
36	23.30163234164602	26.95366823938289	28.195340030773863	21.549359388197228
37	23.92017168039072	29.1456042624097	23.686178044405203	23.248046012794372
38	25.480809714946247	26.71355045152861	26.97510277092355	20.83053706260159
39	26.337170284594443	22.971693659716603	26.76560574241307	23.925530313275885
40	27.390779579015597	25.252429884840428	25.39405089680549	21.962739639338487
41	22.28910589934446	23.99927530869553	30.2020204597707	23.509598332189306
42	25.926086590403962	26.04907997376822	24.49456609108145	23.53026734474637
43	22.595823838771494	25.040636299379166	27.79038048845215	24.573159373397193
44	24.644862984860584	23.524908711861205	25.374657749221086	26.45557055405712
45	26.48108785351029	23.243197725898273	27.414510667507034	22.861203753084403
46	22.38658198325555	23.998254616717404	29.903212883174145	23.7119505168529
47	22.937245305454834	26.513239650821273	26.381825558637477	24.167689485086413
48	22.941583246361873	23.78824724221786	30.801166650931002	22.46900286048927
49	24.388414125356288	23.991109772870516	28.013146512678272	23.607329589094928
50	22.10282961333636	23.152356139845008	27.77277355182946	26.97204069498917
51	22.204388465159955	22.81884503599215	29.77919880783177	25.197567691016125
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	2077.0
1	1675.5
2	1274.0
3	876.5
4	479.0
5	417.0
6	355.0
7	361.5
8	368.0
9	381.5
10	395.0
11	461.5
12	528.0
13	594.5
14	661.0
15	729.5
16	798.0
17	765.5
18	733.0
19	816.5
20	900.0
21	1242.5
22	1585.0
23	1589.0
24	1593.0
25	1810.5
26	2694.0
27	3360.0
28	3874.5
29	4389.0
30	5007.0
31	5625.0
32	7014.5
33	8404.0
34	9275.0
35	10146.0
36	10586.0
37	11026.0
38	11217.5
39	11409.0
40	13267.0
41	15125.0
42	17329.5
43	19534.0
44	26416.0
45	33298.0
46	35985.0
47	38672.0
48	39728.0
49	40784.0
50	37332.5
51	33881.0
52	30875.5
53	27870.0
54	25126.0
55	22382.0
56	20986.0
57	19590.0
58	18578.0
59	17566.0
60	16182.5
61	14799.0
62	13005.5
63	11212.0
64	10155.0
65	9098.0
66	7718.0
67	6338.0
68	5465.0
69	4592.0
70	4024.5
71	3457.0
72	2775.5
73	2094.0
74	1673.5
75	1138.5
76	1024.0
77	690.0
78	356.0
79	384.0
80	412.0
81	366.0
82	320.0
83	183.0
84	46.0
85	38.5
86	31.0
87	24.0
88	17.0
89	9.5
90	2.0
91	3.0
92	4.0
93	2.5
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	391891.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	26.353970581439125
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.41490120723934	19.874820878177232
2	10.322731758045606	5.440899381432425
3	3.6143270153470755	2.857556035024725
4	1.7373149300994015	1.8314058623413836
5	1.038137510226936	1.3679522702004565
6	0.7087056455569889	1.1206324640345224
7	0.48511197324099853	0.894923867004801
8	0.4122451356025802	0.8691436940809424
9	0.33139316063193874	0.7860173045565818
>10	4.396948365392839	28.322665408094487
>50	1.329565317168647	23.925650980265186
>100	0.19564174336715828	7.2177853328845565
>500	0.007985377280292175	1.4073602861335512
>1k	0.004990860800182609	4.083186235769156
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
AATGATACCTGTCTCTTATACACATCTGACGCACATGGTGTCGTATGCCGT	3790	0.9671056492749259	No Hit
AATGATACGGCTGTCTCTTATACACATCTGACGCACATGGTGTCGTATGCC	3748	0.9563883835045969	No Hit
AATCTGTCTCTTATACACATCTGACGCACATGGTGTCGTATGCCGTCTTCT	3405	0.8688640463802435	No Hit
CTGTCTCTTATACACATCTGACGCACATGGTGTCGTATGCCGTCTTCTGCT	2869	0.732091321311283	TruSeq Adapter, Index 21 (95% over 23bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1710	0.43634582064910904	No Hit
AATGACTGTCTCTTATACACATCTGACGCACATGGTGTCGTATGCCGTCTT	912	0.23271777101285815	No Hit
AATGATCTGTCTCTTATACACATCTGACGCACATGGTGTCGTATGCCGTCT	823	0.21000737449954196	No Hit
AAACTGTCTCTTATACACATCTGACGCACATGGTGTCGTATGCCGTCTTCT	737	0.1880624969698207	No Hit
AACTGTCTCTTATACACATCTGACGCACATGGTGTCGTATGCCGTCTTCTG	664	0.16943486836901075	No Hit
AAAAACTGTCTCTTATACACATCTGACGCACATGGTGTCGTATGCCGTCTT	597	0.15233827773539071	No Hit
AATGATACGGCGACTGTCTCTTATACACATCTGACGCACATGGTGTCGTAT	595	0.15182793174632742	No Hit
AATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCACATGGTGTC	520	0.1326899571564542	No Hit
CCTGTCTCTTATACACATCTGACGCACATGGTGTCGTATGCCGTCTTCTGC	502	0.12809684325488466	TruSeq Adapter, Index 14 (95% over 21bp)
AATGCTGTCTCTTATACACATCTGACGCACATGGTGTCGTATGCCGTCTTC	431	0.10997956064313802	No Hit
TGCGAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGG	418	0.10666231171422666	No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	417	0.10640713871969502	No Hit
TGCAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGG	416	0.10615196572516336	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.551729945316427E-4	0.0	0.0	0.8333950001403452	0.0
2	2.551729945316427E-4	0.0	0.0	1.1459819184416076	0.0
3	2.551729945316427E-4	0.0	0.0	1.6343830299751716	0.0
4	2.551729945316427E-4	0.0	0.0	3.923284790924007	0.0
5	2.551729945316427E-4	0.0	0.0	4.3820858350919005	0.0
6	2.551729945316427E-4	0.0	0.0	5.86387541433715	0.0
7	2.551729945316427E-4	0.0	0.0	7.0335884212702	0.0
8	2.551729945316427E-4	0.0	0.0	7.85065234976052	0.0
9	2.551729945316427E-4	0.0	0.0	9.703208290060246	0.0
10	2.551729945316427E-4	0.0	0.0	11.253894577829039	0.0
11	2.551729945316427E-4	0.0	0.0	13.504010043609064	0.0
12	2.551729945316427E-4	0.0	0.0	14.25804624245007	0.0
13	2.551729945316427E-4	0.0	0.0	14.72705420639923	0.0
14	2.551729945316427E-4	0.0	0.0	15.22260016177968	0.0
15	2.551729945316427E-4	0.0	0.0	15.560449206539573	0.0
16	2.551729945316427E-4	0.0	0.0	16.19710582789602	0.0
17	2.551729945316427E-4	0.0	0.0	16.932004052147153	0.0
18	2.551729945316427E-4	0.0	0.0	18.173675843538128	0.0
19	2.551729945316427E-4	0.0	0.0	18.568173293084048	0.0
20	2.551729945316427E-4	0.0	0.0	19.063719248464498	0.0
21	2.551729945316427E-4	0.0	0.0	19.521499600654263	0.0
22	2.551729945316427E-4	0.0	0.0	19.969073033062767	0.0
23	2.551729945316427E-4	0.0	0.0	20.442418937918962	0.0
24	2.551729945316427E-4	0.0	0.0	20.746074801411616	0.0
25	2.551729945316427E-4	0.0	0.0	21.062489314630852	0.0
26	2.551729945316427E-4	0.0	0.0	21.32429680702032	0.0
27	2.551729945316427E-4	0.0	0.0	21.67235277156148	0.0
28	2.551729945316427E-4	0.0	0.0	21.984939689862742	0.0
29	2.551729945316427E-4	0.0	0.0	22.27966449854679	0.0
30	2.551729945316427E-4	0.0	0.0	22.744589694583443	0.0
31	2.551729945316427E-4	0.0	0.0	23.038804157278427	0.0
32	2.551729945316427E-4	0.0	0.0	23.408039480365716	0.0
33	2.551729945316427E-4	0.0	0.0	23.732619529409963	0.0
34	2.551729945316427E-4	0.0	0.0	24.066640979251883	0.0
35	2.551729945316427E-4	0.0	0.0	24.392752066263323	0.0
36	2.551729945316427E-4	0.0	0.0	24.679056166127825	0.0
37	2.551729945316427E-4	0.0	0.0	24.983222375609543	0.0
38	2.551729945316427E-4	0.0	0.0	25.28228512520063	0.0
39	2.551729945316427E-4	0.0	0.0	25.61477553707536	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATCCTCT	20	7.027589E-4	45.000004	37
TGATAAC	20	7.027589E-4	45.000004	27
GATCGGA	20	7.027589E-4	45.000004	25
CGAGTAA	20	7.027589E-4	45.000004	36
CACGGCT	20	7.027589E-4	45.000004	33
TAGACAT	40	6.7957444E-9	45.000004	17
ATTACGG	20	7.027589E-4	45.000004	2
TAGCGAT	20	7.027589E-4	45.000004	45
TAGCGAA	20	7.027589E-4	45.000004	25
CACGCTC	20	7.027589E-4	45.000004	20
CGATTGC	20	7.027589E-4	45.000004	45
TAACGGT	20	7.027589E-4	45.000004	40
CCGCTAC	20	7.027589E-4	45.000004	28
TCTAGCG	20	7.027589E-4	45.000004	1
GCCGCTA	20	7.027589E-4	45.000004	27
TTTCACG	20	7.027589E-4	45.000004	1
TAGCACG	20	7.027589E-4	45.000004	1
CCGTAGT	20	7.027589E-4	45.000004	27
CTAGTAG	20	7.027589E-4	45.000004	1
CAGATAC	20	7.027589E-4	45.000004	14
>>END_MODULE