FastQCFastQC Report
Sat 18 Jun 2016
SRR3548429_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3548429_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences396895
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AATGATACGGCTGTCTCTTATACACATCTGACGCGGACAATCTCGTATGCC37110.9350079995968708No Hit
AATGATACCTGTCTCTTATACACATCTGACGCGGACAATCTCGTATGCCGT35760.9009939656584235No Hit
CTGTCTCTTATACACATCTGACGCGGACAATCTCGTATGCCGTCTTCTGCT31780.8007155544917423RNA PCR Primer, Index 36 (96% over 26bp)
AATCTGTCTCTTATACACATCTGACGCGGACAATCTCGTATGCCGTCTTCT30540.769473034429761RNA PCR Primer, Index 15 (95% over 23bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA15970.4023734237014827No Hit
AAACTGTCTCTTATACACATCTGACGCGGACAATCTCGTATGCCGTCTTCT9470.2386021491830333RNA PCR Primer, Index 15 (95% over 23bp)
AATGACTGTCTCTTATACACATCTGACGCGGACAATCTCGTATGCCGTCTT7780.19602161780823643RNA PCR Primer, Index 15 (95% over 21bp)
AAAAACTGTCTCTTATACACATCTGACGCGGACAATCTCGTATGCCGTCTT7460.1879590319857897RNA PCR Primer, Index 15 (95% over 21bp)
AATGATCTGTCTCTTATACACATCTGACGCGGACAATCTCGTATGCCGTCT6340.1597399816072261No Hit
AACTGTCTCTTATACACATCTGACGCGGACAATCTCGTATGCCGTCTTCTG5610.14134720769976944RNA PCR Primer, Index 36 (95% over 24bp)
AATGATACGGCGACTGTCTCTTATACACATCTGACGCGGACAATCTCGTAT5210.13126897542171104No Hit
AATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGGACAATCTC5100.12849746154524497No Hit
TGCAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGG4780.12043487572279822No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4530.11413598054901171No Hit
CCTGTCTCTTATACACATCTGACGCGGACAATCTCGTATGCCGTCTTCTGC4500.11338011312815731RNA PCR Primer, Index 36 (96% over 25bp)
CTAGAGGGAGCAATAATAGCTCTACTAAACCTATTCTTTTATACTCGCCTA4210.10607339472656495No Hit
AAAAAACTGTCTCTTATACACATCTGACGCGGACAATCTCGTATGCCGTCT4200.1058214389196135No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCACGCG302.1618962E-645.0000041
AACGTAC253.8861734E-545.00000410
AATCCCG253.8861734E-545.0000041
TCGTTCA302.1618962E-645.00000416
CGTATTT253.8861734E-545.00000442
GTACATT302.1618962E-645.00000410
TTCAGTA253.8861734E-545.00000419
AGTCACG253.8861734E-545.0000041
AGATACG302.1618962E-645.0000041
TAGACCG253.8861734E-545.0000041
CGGCATA302.1618962E-645.0000046
CTATACG302.1618962E-645.0000041
GGCCGAT253.8861734E-545.0000048
GCACGGT253.8861734E-545.0000043
TTCGTTG302.1618962E-645.00000432
GCCATAT253.8861734E-545.00000417
CGTTCAT302.1618962E-645.00000417
ATCGTCT302.1618962E-645.00000439
GCCGATG253.8861734E-545.0000049
TACGCGG502.1827873E-1145.0000042
Produced by FastQC (version 0.11.5)