##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3548428_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	369258
Sequences flagged as poor quality	0
Sequence length	51
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.839161236858782	33.0	31.0	34.0	30.0	34.0
2	32.03042317295766	33.0	31.0	34.0	30.0	34.0
3	32.217005454181084	34.0	31.0	34.0	30.0	34.0
4	35.85813983718701	37.0	35.0	37.0	35.0	37.0
5	35.640484430939885	37.0	35.0	37.0	33.0	37.0
6	34.949929317712815	37.0	35.0	37.0	33.0	37.0
7	35.3868162639672	37.0	35.0	37.0	33.0	37.0
8	35.79264904213314	37.0	35.0	37.0	35.0	37.0
9	37.46063998613435	39.0	37.0	39.0	35.0	39.0
10	37.026496379225364	39.0	37.0	39.0	33.0	39.0
11	37.04079803281175	39.0	37.0	39.0	33.0	39.0
12	36.939920055895875	39.0	37.0	39.0	33.0	39.0
13	36.879230781729845	39.0	37.0	39.0	33.0	39.0
14	38.05951936044717	40.0	37.0	41.0	33.0	41.0
15	38.084715835540464	40.0	37.0	41.0	33.0	41.0
16	38.00014082294764	40.0	37.0	41.0	33.0	41.0
17	37.995856555579024	40.0	37.0	41.0	33.0	41.0
18	38.00005416267217	40.0	37.0	41.0	33.0	41.0
19	37.999710229703894	40.0	37.0	41.0	33.0	41.0
20	37.88350150843042	40.0	37.0	41.0	33.0	41.0
21	37.85899019114007	40.0	37.0	41.0	33.0	41.0
22	37.82958798455281	40.0	37.0	41.0	33.0	41.0
23	37.75744330522291	40.0	36.0	41.0	33.0	41.0
24	37.60544659831337	39.0	36.0	41.0	33.0	41.0
25	37.65070492717829	39.0	36.0	41.0	33.0	41.0
26	37.62232368696142	40.0	36.0	41.0	33.0	41.0
27	37.52440299194601	39.0	36.0	41.0	33.0	41.0
28	37.481395122109745	39.0	36.0	41.0	33.0	41.0
29	37.22491320431785	39.0	36.0	41.0	32.0	41.0
30	37.33466844320231	39.0	36.0	41.0	32.0	41.0
31	37.28688071754708	39.0	36.0	41.0	32.0	41.0
32	37.24041185295918	39.0	36.0	41.0	32.0	41.0
33	37.0295403214013	39.0	35.0	41.0	31.0	41.0
34	37.008021491748316	39.0	35.0	41.0	31.0	41.0
35	36.99034821181938	39.0	35.0	41.0	31.0	41.0
36	36.899734061279645	39.0	35.0	41.0	31.0	41.0
37	36.88999290468995	39.0	35.0	41.0	31.0	41.0
38	36.663273375255244	39.0	35.0	40.0	31.0	41.0
39	36.63881892877067	39.0	35.0	40.0	31.0	41.0
40	36.55990933168679	39.0	35.0	40.0	31.0	41.0
41	36.487485714595216	39.0	35.0	40.0	30.0	41.0
42	36.40512866342774	39.0	35.0	40.0	30.0	41.0
43	36.31954622513256	38.0	35.0	40.0	30.0	41.0
44	36.275709666412105	38.0	35.0	40.0	30.0	41.0
45	36.06592138829761	38.0	35.0	40.0	30.0	41.0
46	35.93559787465674	38.0	35.0	40.0	30.0	41.0
47	35.838105064751474	38.0	35.0	40.0	29.0	41.0
48	35.7157326313851	38.0	35.0	40.0	29.0	41.0
49	35.61361432927655	38.0	34.0	40.0	29.0	41.0
50	35.50349620048855	38.0	34.0	40.0	28.0	41.0
51	34.37620038022196	36.0	33.0	40.0	25.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	4.0
10	2.0
11	4.0
12	13.0
13	10.0
14	19.0
15	27.0
16	37.0
17	82.0
18	151.0
19	285.0
20	440.0
21	711.0
22	959.0
23	1218.0
24	1583.0
25	1934.0
26	2353.0
27	2871.0
28	3671.0
29	4640.0
30	6127.0
31	8202.0
32	10868.0
33	15152.0
34	23735.0
35	29947.0
36	35038.0
37	50505.0
38	76910.0
39	91735.0
40	25.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	21.240704331388894	31.281109684827413	20.43503458286618	27.043151400917516
2	24.930265559581645	31.210969024367785	19.90126144863483	23.957503967415736
3	22.797881156264726	30.873264763390367	20.043438463080015	26.285415617264896
4	20.509237443738524	31.54081969788061	19.749605966559965	28.200336891820893
5	19.182522788944315	33.07985202757963	18.507655893711174	29.229969289764878
6	19.241560101609174	39.749443478543455	18.48490757139994	22.524088848447427
7	84.6316125852385	5.47178395593325	5.595545661840773	4.301057796987473
8	86.24918078958343	4.593807040064129	4.164026236398398	4.992985933954038
9	78.91663823126378	8.041261123658797	8.009305147078736	5.0327954979986895
10	35.136408689859124	34.95415129800844	12.993625053485639	16.9158149586468
11	26.456298847959964	28.754150214754997	24.864728726256438	19.924822211028605
12	27.148497798287373	23.175394981286797	29.917564412957876	19.75854280746795
13	25.85211423990814	24.34666276695427	27.843675695584118	21.95754729755347
14	21.80182961506589	27.501909234193977	28.553206701005802	22.143054449734333
15	19.584409816442705	30.631157618792283	27.470765697696464	22.313666867068555
16	21.522079413309935	27.928711090890378	27.70041542769554	22.848794068104144
17	21.847867886410043	26.304372552524253	28.371490935876814	23.476268625188894
18	23.941525979125704	25.485432949319986	25.740809948599626	24.83223112295468
19	23.047300261605706	30.034014158122506	25.796868314295153	21.121817265976635
20	25.480558308824726	27.533052770691498	24.617205314441392	22.369183606042387
21	25.002572726928058	27.861820190760934	25.716707559484153	21.41889952282686
22	22.405201783035167	25.72185301334027	26.79671124254586	25.076233961078703
23	23.782558536307945	27.183161908475917	24.706844536882073	24.327435018334064
24	21.800204734900802	28.3330354386364	25.881362082879722	23.98539774358308
25	22.59964577612401	28.318140703789762	24.535690492826152	24.546523027260072
26	24.37347328967822	27.41579058544432	24.416532614053047	23.79420351082441
27	20.78221731147328	27.24734467499689	25.65739943345845	26.313038580071385
28	21.108276597934235	27.49459727345108	29.54357116162683	21.85355496698785
29	21.86276262125668	31.46797090381251	23.6953566341149	22.973909840815907
30	23.846199676107222	25.967751544990225	26.55704141819541	23.629007360707146
31	24.356953674666492	27.454246082684737	24.650786171186542	23.53801407146223
32	23.2149337319706	27.74618288567885	25.686105649708335	23.35277773264222
33	23.513640868985913	24.860395712482873	27.272801130916598	24.353162287614623
34	21.47495788852239	26.85926912890174	27.954709173531782	23.711063809044084
35	24.109701076212296	25.317799478955095	26.80754377697978	23.76495566785283
36	24.275168039690406	28.240146455865546	25.58346738594695	21.901218118497095
37	23.71160543576578	29.51513575873779	23.485476279457725	23.287782526038704
38	23.83184656798228	28.718673664483912	25.715895119401612	21.7335846481322
39	24.763444529299296	24.542190013486508	26.008102735756573	24.686262721457624
40	26.742548570376268	24.554918241446362	25.430999463789544	23.271533724387826
41	23.255555736097797	24.074766152662907	28.545082300180365	24.124595811058935
42	24.40786658650591	25.700729571194124	25.94581566276154	23.945588179538426
43	21.80426693531352	25.10791912429792	27.547947505538133	25.539866434850428
44	23.860011157510467	22.884812245096924	26.51425290718143	26.740923690211183
45	24.959242589192378	24.900746903249217	27.507054688050093	22.63295581950831
46	21.51070525215432	25.17399758434482	28.34684692003965	24.968450243461213
47	22.689826625286386	26.025976417572537	26.69569785894957	24.58849909819151
48	23.09740073336258	24.87122824691679	29.001402813209193	23.029968206511438
49	23.16537488693542	24.32147712439541	28.31841151715061	24.194736471518556
50	22.43444962600675	24.199340298652974	26.952699738394294	26.413510336945983
51	22.71582470792779	22.14224200965179	29.66922856106029	25.472704721360135
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1440.0
1	1182.5
2	925.0
3	618.5
4	312.0
5	300.5
6	289.0
7	303.0
8	317.0
9	371.5
10	426.0
11	385.0
12	344.0
13	430.0
14	516.0
15	557.5
16	599.0
17	693.5
18	788.0
19	685.0
20	582.0
21	784.5
22	987.0
23	1226.0
24	1465.0
25	1702.5
26	2748.0
27	3556.0
28	4100.5
29	4645.0
30	5185.5
31	5726.0
32	6058.5
33	6391.0
34	7270.5
35	8150.0
36	9609.5
37	11069.0
38	11639.5
39	12210.0
40	13756.5
41	15303.0
42	16934.0
43	18565.0
44	21157.5
45	23750.0
46	27119.0
47	30488.0
48	32639.0
49	34790.0
50	34008.0
51	33226.0
52	30635.5
53	28045.0
54	25654.0
55	23263.0
56	21441.0
57	19619.0
58	18330.5
59	17042.0
60	16115.5
61	15189.0
62	14367.0
63	13545.0
64	11865.0
65	10185.0
66	9022.5
67	7860.0
68	6517.5
69	5175.0
70	4456.5
71	3738.0
72	3125.0
73	2512.0
74	2245.0
75	1495.5
76	1013.0
77	856.5
78	700.0
79	541.0
80	382.0
81	244.0
82	106.0
83	72.0
84	38.0
85	32.5
86	27.0
87	25.5
88	24.0
89	16.5
90	9.0
91	6.0
92	3.0
93	3.5
94	4.0
95	2.5
96	1.0
97	1.0
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	369258.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	27.33609018550412
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.27187281486745	20.849747938845166
2	9.60618004267671	5.251908079696007
3	3.380808910620882	2.7725429184206516
4	1.5838738466990034	1.7318767326330118
5	0.9999196926659369	1.3666947448488806
6	0.6469480287785707	1.0611017796015076
7	0.4599630495740646	0.8801513983609294
8	0.391968511804741	0.857190927085779
9	0.333973170772255	0.8216568644192219
>10	4.845610735216545	32.50683386595298
>50	1.3288932453782065	24.624558749766422
>100	0.14398843290779664	5.737111086924424
>500	0.0039996786918832395	0.6253995029245358
>1k	0.0019998393459416198	0.9132254105204869
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTGTCTCTTATACACATCTGACGCTACTAGGGTCGTATGCCGTCTTCTGCT	2175	0.5890190598443366	Illumina Single End Adapter 2 (95% over 21bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1166	0.3157683787487339	No Hit
AAACTGTCTCTTATACACATCTGACGCTACTAGGGTCGTATGCCGTCTTCT	748	0.20256839391428216	No Hit
AAAAACTGTCTCTTATACACATCTGACGCTACTAGGGTCGTATGCCGTCTT	526	0.14244782780603263	No Hit
TGCAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGG	507	0.1373023739499212	No Hit
CCTGTCTCTTATACACATCTGACGCTACTAGGGTCGTATGCCGTCTTCTGC	507	0.1373023739499212	No Hit
AATGATACCTGTCTCTTATACACATCTGACGCTACTAGGGTCGTATGCCGT	460	0.12457414599006655	No Hit
AATCTGTCTCTTATACACATCTGACGCTACTAGGGTCGTATGCCGTCTTCT	457	0.12376170590752265	No Hit
AATGATACGGCTGTCTCTTATACACATCTGACGCTACTAGGGTCGTATGCC	454	0.12294926582497874	No Hit
TGCGAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGG	420	0.11374161155614773	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.6615970405515926	0.0
2	0.0	0.0	0.0	0.9478467629678978	0.0
3	0.0	0.0	0.0	1.2489912202308413	0.0
4	0.0	0.0	0.0	2.6845728460859344	0.0
5	0.0	0.0	0.0	2.91341013600247	0.0
6	0.0	0.0	0.0	4.054617638615818	0.0
7	0.0	0.0	0.0	4.917699819638302	0.0
8	0.0	0.0	0.0	5.59310834159314	0.0
9	0.0	0.0	0.0	6.58482686901841	0.0
10	0.0	0.0	0.0	8.04613576415406	0.0
11	0.0	0.0	0.0	9.230673404503085	0.0
12	0.0	0.0	0.0	9.829441745337949	0.0
13	0.0	0.0	0.0	10.076694343792145	0.0
14	0.0	0.0	0.0	10.36429813301269	0.0
15	0.0	0.0	0.0	10.619404318931478	0.0
16	0.0	0.0	0.0	11.155343960049613	0.0
17	0.0	0.0	0.0	11.79365105156828	0.0
18	0.0	0.0	0.0	12.75314278905264	0.0
19	0.0	0.0	0.0	13.108449918485178	0.0
20	0.0	0.0	0.0	13.527398187716988	0.0
21	0.0	0.0	0.0	13.951221097444064	0.0
22	0.0	0.0	0.0	14.371523433480114	0.0
23	0.0	0.0	0.0	14.822698492652833	0.0
24	0.0	0.0	0.0	15.149841032557182	0.0
25	0.0	0.0	0.0	15.42634147398296	0.0
26	0.0	0.0	0.0	15.683072540066837	0.0
27	0.0	0.0	0.0	15.982050490442996	0.0
28	0.0	0.0	0.0	16.294298295500706	0.0
29	0.0	0.0	0.0	16.626857102622015	0.0
30	0.0	0.0	0.0	17.08778144278526	0.0
31	0.0	0.0	0.0	17.374572791923264	0.0
32	0.0	0.0	0.0	17.68059188968147	0.0
33	0.0	0.0	0.0	17.999880842121225	0.0
34	0.0	0.0	0.0	18.29831716577569	0.0
35	0.0	0.0	0.0	18.611377410915946	0.0
36	0.0	0.0	0.0	18.92308358925196	0.0
37	0.0	0.0	0.0	19.200396470760282	0.0
38	0.0	0.0	0.0	19.513456715900535	0.0
39	0.0	0.0	0.0	19.85738968417746	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATCCCGC	35	1.2089367E-7	45.000004	44
TAGGGTA	65	0.0	45.000004	5
AGTAGGA	35	1.2089367E-7	45.000004	28
CGTGGTT	20	7.027061E-4	45.0	32
CGAACCG	20	7.027061E-4	45.0	44
CTCGTGG	25	3.885646E-5	45.0	2
GTACATC	20	7.027061E-4	45.0	36
CTCAACG	20	7.027061E-4	45.0	1
GTCGAAC	20	7.027061E-4	45.0	11
GTCTGCG	20	7.027061E-4	45.0	44
CGAGTAG	20	7.027061E-4	45.0	27
CGAAACG	20	7.027061E-4	45.0	1
CCGATAT	20	7.027061E-4	45.0	28
GTTTAGG	25	3.885646E-5	45.0	2
GTTTACC	25	3.885646E-5	45.0	41
ATAATCG	20	7.027061E-4	45.0	28
GGCCGAT	45	3.8380676E-10	45.0	8
ATAGCCC	20	7.027061E-4	45.0	25
TAAACGT	20	7.027061E-4	45.0	21
TAAACGG	95	0.0	45.0	2
>>END_MODULE