SRR3548427_1.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR3548427_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	376282
Sequences flagged as poor quality	0
Sequence length	51
%GC	49

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
CTGTCTCTTATACACATCTGACGCTACTAGGGTCGTATGCCGTCTTCTGCT	2388	0.6346304101710951	Illumina Single End Adapter 2 (95% over 21bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1212	0.32209885139336986	No Hit
AAACTGTCTCTTATACACATCTGACGCTACTAGGGTCGTATGCCGTCTTCT	827	0.2197819720316146	No Hit
AAAAACTGTCTCTTATACACATCTGACGCTACTAGGGTCGTATGCCGTCTT	620	0.16477003949165786	No Hit
AATCTGTCTCTTATACACATCTGACGCTACTAGGGTCGTATGCCGTCTTCT	574	0.1525451655938897	No Hit
AATGATACGGCTGTCTCTTATACACATCTGACGCTACTAGGGTCGTATGCC	558	0.14829303554249207	No Hit
AATGATACCTGTCTCTTATACACATCTGACGCTACTAGGGTCGTATGCCGT	542	0.14404090549109444	No Hit
CCTGTCTCTTATACACATCTGACGCTACTAGGGTCGTATGCCGTCTTCTGC	497	0.13208178972153864	No Hit
TGCAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGG	475	0.1262351109008669	No Hit
TGCGAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGG	427	0.11347872074667403	No Hit

Adapter graph

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCATACG	35	1.2090095E-7	45.000004	1
CGGACAA	20	7.027232E-4	45.0	27
TCTGCGT	20	7.027232E-4	45.0	18
TCGTTTA	20	7.027232E-4	45.0	18
GTCGCGC	20	7.027232E-4	45.0	10
AGATCGT	20	7.027232E-4	45.0	19
AGCCCGT	20	7.027232E-4	45.0	17
GTCTCGA	25	3.8857877E-5	45.0	13
CGACCTA	25	3.8857877E-5	45.0	29
CACGCGT	25	3.8857877E-5	45.0	42
ATTCGAA	20	7.027232E-4	45.0	16
TAACGTC	20	7.027232E-4	45.0	32
AAGCGCG	20	7.027232E-4	45.0	1
CGCGACG	45	3.8380676E-10	45.0	1
TAACGCA	25	3.8857877E-5	45.0	27
ATCACGC	40	6.7939254E-9	45.0	42
TCGGCAC	20	7.027232E-4	45.0	27
ATCCGTC	25	3.8857877E-5	45.0	28
CGTTACG	25	3.8857877E-5	45.0	1
ACTAGTG	20	7.027232E-4	45.0	12