##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3548427_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	376282
Sequences flagged as poor quality	0
Sequence length	51
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.83002375877666	33.0	31.0	34.0	30.0	34.0
2	32.02471816350503	33.0	31.0	34.0	30.0	34.0
3	32.18954135462233	34.0	31.0	34.0	30.0	34.0
4	35.840794935713106	37.0	35.0	37.0	35.0	37.0
5	35.62628028978266	37.0	35.0	37.0	33.0	37.0
6	35.65299695441185	37.0	35.0	37.0	33.0	37.0
7	35.73947730691343	37.0	35.0	37.0	35.0	37.0
8	35.78233346266896	37.0	35.0	37.0	35.0	37.0
9	37.484410628199065	39.0	37.0	39.0	35.0	39.0
10	37.017699491338945	39.0	37.0	39.0	33.0	39.0
11	37.039231214886705	39.0	37.0	39.0	33.0	39.0
12	36.97229737271515	39.0	37.0	39.0	33.0	39.0
13	36.9043058131933	39.0	37.0	39.0	33.0	39.0
14	38.07381697769226	40.0	37.0	41.0	33.0	41.0
15	38.09664028574314	40.0	37.0	41.0	33.0	41.0
16	38.01140633886288	40.0	37.0	41.0	33.0	41.0
17	37.971646265301025	40.0	37.0	41.0	33.0	41.0
18	37.95004012947736	40.0	37.0	41.0	33.0	41.0
19	37.97498152981009	40.0	37.0	41.0	33.0	41.0
20	37.86944100435312	40.0	37.0	41.0	33.0	41.0
21	37.8265104363217	40.0	37.0	41.0	33.0	41.0
22	37.79224358326999	40.0	36.0	41.0	33.0	41.0
23	37.67731648072456	40.0	36.0	41.0	33.0	41.0
24	37.553398780701706	39.0	36.0	41.0	32.0	41.0
25	37.6129844106282	39.0	36.0	41.0	33.0	41.0
26	37.55127005809473	39.0	36.0	41.0	33.0	41.0
27	37.49743543406275	39.0	36.0	41.0	33.0	41.0
28	37.43048830398478	39.0	36.0	41.0	32.0	41.0
29	37.23711737473491	39.0	36.0	41.0	32.0	41.0
30	37.32918396309151	39.0	36.0	41.0	32.0	41.0
31	37.26440807692103	39.0	36.0	41.0	32.0	41.0
32	37.201973519860104	39.0	36.0	41.0	32.0	41.0
33	37.13847858786761	39.0	35.0	41.0	32.0	41.0
34	37.07211878325299	39.0	35.0	41.0	31.0	41.0
35	37.02638180938764	39.0	35.0	41.0	31.0	41.0
36	36.88112107408805	39.0	35.0	41.0	31.0	41.0
37	36.867721549263585	39.0	35.0	41.0	31.0	41.0
38	36.75866238618908	39.0	35.0	40.0	31.0	41.0
39	36.66128063526823	39.0	35.0	40.0	31.0	41.0
40	36.555862358550236	39.0	35.0	40.0	31.0	41.0
41	36.44395161076002	39.0	35.0	40.0	30.0	41.0
42	36.35524952030658	39.0	35.0	40.0	30.0	41.0
43	36.23811131013442	38.0	35.0	40.0	30.0	41.0
44	36.19653876613816	38.0	35.0	40.0	30.0	41.0
45	36.00725253931891	38.0	35.0	40.0	30.0	41.0
46	35.8957271408146	38.0	35.0	40.0	30.0	41.0
47	35.755250051822834	38.0	35.0	40.0	29.0	41.0
48	35.65723048139427	38.0	34.0	40.0	29.0	41.0
49	35.47657873616065	38.0	34.0	40.0	28.0	41.0
50	35.38025470259008	37.0	34.0	40.0	28.0	41.0
51	34.183051541131384	36.0	33.0	40.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	3.0
11	5.0
12	10.0
13	11.0
14	18.0
15	29.0
16	42.0
17	102.0
18	159.0
19	245.0
20	477.0
21	675.0
22	958.0
23	1270.0
24	1572.0
25	1916.0
26	2439.0
27	2989.0
28	3911.0
29	4910.0
30	6394.0
31	8413.0
32	11108.0
33	15227.0
34	24334.0
35	30329.0
36	35005.0
37	50755.0
38	78922.0
39	94021.0
40	32.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	21.22796200721799	31.461244492162795	20.335546212680917	26.975247287938302
2	24.857420764214076	31.484896965573693	19.924152630208194	23.73352964000404
3	22.730292706002412	30.86089688053109	20.101944817982258	26.306865595484236
4	20.646217464561154	31.550804981370355	19.656002678841933	28.14697487522656
5	19.188268373188194	33.12329582600284	18.663130311840586	29.025305488968378
6	19.888009524771316	38.16605630883221	18.78883390648503	23.157100259911452
7	84.0951733008754	5.597397696408544	5.8514624669795525	4.455966535736495
8	85.56136089422294	4.708170999410017	4.39324761747838	5.3372204888886525
9	78.53471598428838	8.022174858218039	8.301220892841007	5.141888264652574
10	34.8244136046901	34.806873568228085	13.164063122870612	17.204649704211203
11	26.33211261766441	28.437714267490872	25.26642252353288	19.963750591311836
12	26.927145066731867	23.078435853960595	30.354893404414774	19.639525674892766
13	25.599151700054744	24.339723930456415	28.066184404250006	21.994939965238835
14	21.64945439856278	27.334020761024973	28.80977564698816	22.20674919342408
15	19.4444592087849	30.549959870522642	27.55061363551804	22.45496728517442
16	21.334531016631146	27.998681839684064	27.6508044498541	23.01598269383069
17	21.538101742841807	26.471635634975893	28.461898257158197	23.528364365024103
18	23.772064568594832	25.328344167406357	25.890954124831904	25.0086371391669
19	22.639669184282003	30.25496834820693	26.1274788589409	20.97788360857017
20	25.315056260995743	27.48257955469568	24.86592502431687	22.33643915999171
21	24.695839822260965	27.994163951504458	25.856405568164302	21.453590658070276
22	22.36726710286434	25.702265853801137	26.917577774116218	25.0128892692183
23	23.774987908005166	27.330034389101794	24.53505615469249	24.359921548200553
24	21.364030168862715	28.537639323698716	25.89228291547297	24.2060475919656
25	22.509979217714374	28.20730197033076	24.68202039959392	24.60069841236094
26	24.375335519636867	27.307976464460165	24.46223842756231	23.85444958834066
27	20.510680819172855	27.35129503935878	25.629448126670955	26.508576014797413
28	20.97602330167268	27.3345522772814	29.767036424809053	21.922387996236864
29	21.768514040001914	31.529544331113367	23.592146315795066	23.109795313089652
30	23.653004927155695	25.873945604626318	26.752541976496353	23.720507491721634
31	24.274347430916173	27.50463747933731	24.68334919023498	23.537665899511538
32	23.16826210129637	27.821421168166427	25.5938365374905	23.416480193046706
33	23.519062830536672	24.656507619285538	27.346245634922745	24.478183915255048
34	21.6348377015111	26.708957643469528	27.91204468988684	23.744159965132532
35	24.19488575058068	25.21220786537756	26.824296671113686	23.768609712928072
36	24.29534232304495	28.030041298813124	25.664528199594983	22.01008817854694
37	23.674265577412683	29.436167555184678	23.5326164950755	23.356950372327137
38	23.865079913469152	28.528603547339493	25.925502681499513	21.680813857691838
39	24.666606428157607	24.460643878793036	26.092664544145084	24.78008514890428
40	26.789216598189658	24.60601357492519	25.326483860508876	23.27828596637628
41	23.417808983687767	23.86135929967418	28.566341201545647	24.1544905150924
42	24.65889944243945	25.706517983852535	25.844712210522957	23.78987036318506
43	21.848772994722044	24.842538309034182	27.588351289724194	25.720337406519576
44	23.883417224315806	22.60432335322976	26.541795780823957	26.970463641630477
45	25.17606475994068	24.70540711487661	27.478858940900707	22.639669184282003
46	21.512854720661633	25.06604089486077	28.446218527593665	24.974885856883933
47	22.81002014446612	25.893611706114033	26.738191037572882	24.558177111846966
48	23.200418834810062	24.72268139321041	29.118586591970914	22.95831318000861
49	23.01598269383069	24.100541615065296	28.446750043850088	24.436725647253922
50	22.38374410681351	23.985202587421135	26.98614337119501	26.64490993457035
51	22.816398339543216	21.808112001105552	29.718668445474407	25.656821213876825
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1472.0
1	1172.5
2	873.0
3	623.5
4	374.0
5	331.0
6	288.0
7	310.0
8	332.0
9	396.0
10	460.0
11	403.0
12	346.0
13	451.0
14	556.0
15	585.5
16	615.0
17	716.0
18	817.0
19	718.0
20	619.0
21	824.5
22	1030.0
23	1253.5
24	1477.0
25	1686.0
26	2738.5
27	3582.0
28	4129.0
29	4676.0
30	5184.0
31	5692.0
32	6085.5
33	6479.0
34	7387.0
35	8295.0
36	9670.5
37	11046.0
38	11634.5
39	12223.0
40	13674.5
41	15126.0
42	16992.5
43	18859.0
44	21905.5
45	24952.0
46	28278.0
47	31604.0
48	33998.5
49	36393.0
50	35194.0
51	33995.0
52	31225.5
53	28456.0
54	26064.0
55	23672.0
56	21689.0
57	19706.0
58	18406.5
59	17107.0
60	16286.5
61	15466.0
62	14585.5
63	13705.0
64	12042.0
65	10379.0
66	9142.0
67	7905.0
68	6577.5
69	5250.0
70	4538.0
71	3826.0
72	3161.0
73	2496.0
74	2170.5
75	1453.5
76	1062.0
77	872.0
78	682.0
79	539.5
80	397.0
81	254.5
82	112.0
83	83.5
84	55.0
85	45.0
86	35.0
87	28.0
88	21.0
89	20.5
90	20.0
91	12.0
92	4.0
93	3.5
94	3.0
95	2.0
96	1.0
97	1.0
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	376282.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	26.858083903230167
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.97903867667564	20.406513956649246
2	9.74646574876615	5.2354278968064065
3	3.380634115649303	2.7239206417269393
4	1.6266861151426912	1.7475868865888773
5	0.9828066450419386	1.3198151666594267
6	0.6748671146373297	1.0875382553076152
7	0.4669080595104437	0.8778179086198117
8	0.401920856232078	0.8635859263311408
9	0.3369336531049492	0.8144453092423117
>10	4.863042399707227	31.904344240563887
>50	1.39372555616609	25.592325961949797
>100	0.1399724374915729	5.621901415853996
>500	0.004999015624699032	0.8380757699121284
>1k	0.001999606249879613	0.9667006637884211
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTGTCTCTTATACACATCTGACGCTACTAGGGTCGTATGCCGTCTTCTGCT	2388	0.6346304101710951	Illumina Single End Adapter 2 (95% over 21bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1212	0.32209885139336986	No Hit
AAACTGTCTCTTATACACATCTGACGCTACTAGGGTCGTATGCCGTCTTCT	827	0.2197819720316146	No Hit
AAAAACTGTCTCTTATACACATCTGACGCTACTAGGGTCGTATGCCGTCTT	620	0.16477003949165786	No Hit
AATCTGTCTCTTATACACATCTGACGCTACTAGGGTCGTATGCCGTCTTCT	574	0.1525451655938897	No Hit
AATGATACGGCTGTCTCTTATACACATCTGACGCTACTAGGGTCGTATGCC	558	0.14829303554249207	No Hit
AATGATACCTGTCTCTTATACACATCTGACGCTACTAGGGTCGTATGCCGT	542	0.14404090549109444	No Hit
CCTGTCTCTTATACACATCTGACGCTACTAGGGTCGTATGCCGTCTTCTGC	497	0.13208178972153864	No Hit
TGCAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGG	475	0.1262351109008669	No Hit
TGCGAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGG	427	0.11347872074667403	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.726051206276144	0.0
2	0.0	0.0	0.0	1.0431006532334792	0.0
3	0.0	0.0	0.0	1.3891177361659606	0.0
4	0.0	0.0	0.0	2.978351342875822	0.0
5	0.0	0.0	0.0	3.2175336582669383	0.0
6	0.0	0.0	0.0	4.467925651506051	0.0
7	0.0	0.0	0.0	5.378412998761567	0.0
8	0.0	0.0	0.0	6.082140522267873	0.0
9	0.0	0.0	0.0	7.118065706039619	0.0
10	0.0	0.0	0.0	8.701186875800596	0.0
11	0.0	0.0	0.0	9.998883815861507	0.0
12	0.0	0.0	0.0	10.684539786649374	0.0
13	0.0	0.0	0.0	10.93727576657932	0.0
14	0.0	0.0	0.0	11.246352469690285	0.0
15	0.0	0.0	0.0	11.495899352081683	0.0
16	0.0	0.0	0.0	12.039374724275943	0.0
17	0.0	0.0	0.0	12.710413998012129	0.0
18	0.0	0.0	0.0	13.695579379295316	0.0
19	0.0	0.0	0.0	14.076145018895403	0.0
20	0.0	0.0	0.0	14.507470460984049	0.0
21	0.0	0.0	0.0	14.939858935585544	0.0
22	0.0	0.0	0.0	15.392179269802966	0.0
23	0.0	0.0	0.0	15.85034628284106	0.0
24	0.0	0.0	0.0	16.184404250003986	0.0
25	0.0	0.0	0.0	16.463184526498743	0.0
26	0.0	0.0	0.0	16.726550831557184	0.0
27	0.0	0.0	0.0	17.055559394284074	0.0
28	0.0	0.0	0.0	17.373140357497835	0.0
29	0.0	0.0	0.0	17.69816254830154	0.0
30	0.0	0.0	0.0	18.16403654705779	0.0
31	0.0	0.0	0.0	18.47231597578412	0.0
32	0.0	0.0	0.0	18.79547785969034	0.0
33	0.0	0.0	0.0	19.120234292365833	0.0
34	0.0	0.0	0.0	19.4229328003997	0.0
35	0.0	0.0	0.0	19.769481399588607	0.0
36	0.0	0.0	0.0	20.075634763289234	0.0
37	0.0	0.0	0.0	20.35866716983539	0.0
38	0.0	0.0	0.0	20.66482053353602	0.0
39	0.0	0.0	0.0	21.00180184010928	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCATACG	35	1.2090095E-7	45.000004	1
CGGACAA	20	7.027232E-4	45.0	27
TCTGCGT	20	7.027232E-4	45.0	18
TCGTTTA	20	7.027232E-4	45.0	18
GTCGCGC	20	7.027232E-4	45.0	10
AGATCGT	20	7.027232E-4	45.0	19
AGCCCGT	20	7.027232E-4	45.0	17
GTCTCGA	25	3.8857877E-5	45.0	13
CGACCTA	25	3.8857877E-5	45.0	29
CACGCGT	25	3.8857877E-5	45.0	42
ATTCGAA	20	7.027232E-4	45.0	16
TAACGTC	20	7.027232E-4	45.0	32
AAGCGCG	20	7.027232E-4	45.0	1
CGCGACG	45	3.8380676E-10	45.0	1
TAACGCA	25	3.8857877E-5	45.0	27
ATCACGC	40	6.7939254E-9	45.0	42
TCGGCAC	20	7.027232E-4	45.0	27
ATCCGTC	25	3.8857877E-5	45.0	28
CGTTACG	25	3.8857877E-5	45.0	1
ACTAGTG	20	7.027232E-4	45.0	12
>>END_MODULE