FastQCFastQC Report
Sat 18 Jun 2016
SRR3548426_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3548426_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences259564
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTCTCTTATACACATCTGACGCTTGCTTCGTCGTATGCCGTCTTCTGCT19850.7647439552480313No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA16960.6534033995469326No Hit
AATGATACCTGTCTCTTATACACATCTGACGCTTGCTTCGTCGTATGCCGT12400.477724183631012No Hit
AATGATACGGCTGTCTCTTATACACATCTGACGCTTGCTTCGTCGTATGCC12110.4665516019170609No Hit
AATCTGTCTCTTATACACATCTGACGCTTGCTTCGTCGTATGCCGTCTTCT11440.4407390855434498No Hit
AAACTGTCTCTTATACACATCTGACGCTTGCTTCGTCGTATGCCGTCTTCT6650.25619885654405083No Hit
AAAAACTGTCTCTTATACACATCTGACGCTTGCTTCGTCGTATGCCGTCTT4980.19186019632922902No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4890.18839284338352005No Hit
CCTGTCTCTTATACACATCTGACGCTTGCTTCGTCGTATGCCGTCTTCTGC4580.17644973879274475No Hit
GCTCTAGGGATTTGGAGAAAGAAGATTAGAAAATGGAAGTGAAAGACGAAG3540.1363825491978857No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3200.12328366029187407No Hit
AATGACTGTCTCTTATACACATCTGACGCTTGCTTCGTCGTATGCCGTCTT3080.11866052303092878No Hit
AAAAAACTGTCTCTTATACACATCTGACGCTTGCTTCGTCGTATGCCGTCT2780.1071026798785656No Hit
AATGATCTGTCTCTTATACACATCTGACGCTTGCTTCGTCGTATGCCGTCT2720.10479111124809297No Hit
AACTGTCTCTTATACACATCTGACGCTTGCTTCGTCGTATGCCGTCTTCTG2680.10325006549444453No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCCCA207.023184E-445.00000417
CGGGTCC207.023184E-445.0000046
CTAGAAT207.023184E-445.00000428
GCCCATT207.023184E-445.00000440
AGTCCGT207.023184E-445.00000437
ATCTACA207.023184E-445.00000410
GTCGAAC207.023184E-445.00000436
ATAGGCG207.023184E-445.0000041
CACGGAG207.023184E-445.00000422
GCGAATG207.023184E-445.0000041
GAGTCCG207.023184E-445.00000436
GGGTCGT207.023184E-445.0000048
GATCCGT207.023184E-445.00000430
ACTTCGC207.023184E-445.00000414
GTTAGGT207.023184E-445.00000418
TTCAACG207.023184E-445.0000041
TGAGTCC207.023184E-445.00000414
ACCTCGT207.023184E-445.00000410
ATCCGTT207.023184E-445.00000431
CGTTAGC207.023184E-445.00000432