##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3548426_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	259564
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.692607603519747	31.0	31.0	34.0	30.0	34.0
2	31.891964987440478	33.0	31.0	34.0	30.0	34.0
3	32.06299024518038	34.0	31.0	34.0	30.0	34.0
4	35.736824058806306	37.0	35.0	37.0	33.0	37.0
5	35.512740595768285	37.0	35.0	37.0	33.0	37.0
6	34.880765437425836	37.0	35.0	37.0	32.0	37.0
7	35.30737698602272	37.0	35.0	37.0	33.0	37.0
8	35.727462205852895	37.0	35.0	37.0	35.0	37.0
9	37.362573392304014	39.0	37.0	39.0	34.0	39.0
10	36.89818695967083	39.0	37.0	39.0	33.0	39.0
11	36.950809819543544	39.0	37.0	39.0	33.0	39.0
12	36.96220585289177	39.0	37.0	39.0	33.0	39.0
13	36.955459925105174	39.0	37.0	39.0	33.0	39.0
14	38.10217133346689	40.0	37.0	41.0	33.0	41.0
15	38.15187776425082	40.0	37.0	41.0	33.0	41.0
16	37.98622305096238	40.0	37.0	41.0	33.0	41.0
17	37.99099644018431	40.0	37.0	41.0	33.0	41.0
18	37.90119585150483	40.0	37.0	41.0	33.0	41.0
19	37.881485876315665	40.0	37.0	41.0	33.0	41.0
20	37.847760089997074	40.0	37.0	41.0	33.0	41.0
21	37.813182875899585	40.0	37.0	41.0	33.0	41.0
22	37.79559954385046	39.0	37.0	41.0	33.0	41.0
23	37.67506665022884	39.0	37.0	41.0	33.0	41.0
24	37.602441016473776	39.0	36.0	41.0	32.0	41.0
25	37.56719730008784	39.0	36.0	41.0	32.0	41.0
26	37.54509099875175	39.0	36.0	41.0	33.0	41.0
27	37.468724476429706	39.0	36.0	41.0	32.0	41.0
28	37.41137831132206	39.0	36.0	41.0	32.0	41.0
29	37.20643848915874	39.0	36.0	41.0	31.0	41.0
30	37.26191613629009	39.0	36.0	41.0	32.0	41.0
31	37.231330230694546	39.0	36.0	41.0	32.0	41.0
32	37.199018353854925	39.0	36.0	41.0	32.0	41.0
33	36.984477816646375	39.0	36.0	41.0	31.0	41.0
34	37.0071080735387	39.0	36.0	41.0	31.0	41.0
35	36.940072583254995	39.0	36.0	41.0	31.0	41.0
36	36.928010047618315	39.0	36.0	41.0	31.0	41.0
37	36.863255305050004	39.0	35.0	41.0	31.0	41.0
38	36.69450308979673	39.0	35.0	41.0	30.0	41.0
39	36.64903838744972	39.0	35.0	41.0	30.0	41.0
40	36.54128461574024	39.0	35.0	40.0	30.0	41.0
41	36.543885130449524	39.0	35.0	40.0	30.0	41.0
42	36.44204897443405	39.0	35.0	40.0	30.0	41.0
43	36.39055493057589	39.0	35.0	40.0	30.0	41.0
44	36.29922485398591	39.0	35.0	40.0	30.0	41.0
45	36.072298161532416	39.0	35.0	40.0	29.0	41.0
46	35.9196190534897	38.0	35.0	40.0	29.0	41.0
47	35.788622459200816	38.0	35.0	40.0	28.0	41.0
48	35.69110893652432	38.0	35.0	40.0	28.0	41.0
49	35.58845602625942	38.0	34.0	40.0	28.0	41.0
50	35.47632183199519	38.0	34.0	40.0	27.0	41.0
51	34.224044936894174	37.0	33.0	40.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	1.0
11	0.0
12	2.0
13	2.0
14	3.0
15	12.0
16	19.0
17	47.0
18	113.0
19	246.0
20	430.0
21	710.0
22	901.0
23	1202.0
24	1397.0
25	1605.0
26	1842.0
27	2151.0
28	2659.0
29	3383.0
30	4353.0
31	5867.0
32	7544.0
33	10147.0
34	14470.0
35	19760.0
36	24004.0
37	35447.0
38	55979.0
39	65254.0
40	13.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	19.86215345733615	34.71706399963015	20.863833197207626	24.556949345826077
2	21.765344963091955	32.311491578184956	20.756730517329057	25.166432941394028
3	19.47997411043134	32.9302214482748	22.401411597910343	25.18839284338352
4	19.577059992911188	32.96296867053983	19.911081660014485	27.548889676534493
5	17.749764990522568	35.8420273997935	18.7244764297052	27.683731179978732
6	19.486138293445933	37.61268897073554	20.51208950393737	22.389083231881155
7	82.18397004207056	7.420520565255583	5.980798569909541	4.414710822764327
8	84.01742922747376	5.467630333944615	4.398529842351019	6.116410596230602
9	76.81920451218197	10.566565471328843	8.27233360558475	4.34189641090444
10	27.782743369650646	47.039651107241376	11.809418871646299	13.368186651461683
11	19.524664437287143	28.898460495292106	33.560509161517004	18.016365905903747
12	21.862045584133394	25.177220261669568	33.999321939868395	18.961412214328643
13	23.945924704504478	27.269960395124134	28.32326516774283	20.46084973262856
14	17.654220153796366	31.065556086360203	30.934181935861677	20.346041823981754
15	18.14157587338768	31.71587739439984	29.423571835847806	20.71897489636467
16	20.562944013807773	32.486785532662466	26.822286603689268	20.127983849840504
17	19.31354116903731	29.868163535775377	27.72842150683454	23.089873788352776
18	22.117859179239034	28.477369743107673	26.960980721517625	22.443790356135672
19	20.7420905826694	32.14274706816045	28.425359448922038	18.68980290024811
20	21.035659798739424	30.38980752338537	27.181350264289346	21.39318241358586
21	22.00613336209952	29.947142130649855	27.860180918771476	20.186543588479143
22	19.756206561772817	28.509346442495875	29.041007227504583	22.69343976822672
23	20.280547379451697	29.54724075757809	27.362808401781447	22.80940346118876
24	19.348599959932812	30.01263657517992	27.28305928403014	23.355704180857128
25	20.0802114314774	31.063244517729732	26.773358401010928	22.083185649781942
26	21.972230355519258	30.414464255443747	26.712101832303404	20.9012035567336
27	20.171518392381067	29.353839515495217	26.53950470789478	23.935137384228938
28	20.684301366907583	28.286280069655266	29.826170038988458	21.20324852444869
29	21.939097871815814	28.62685118121157	28.78789046246783	20.646160484504787
30	22.54704042163012	27.27496879382349	28.36333235733769	21.814658427208705
31	21.165107642045893	29.22670324081922	25.479265229384662	24.12892388775023
32	23.015133069300827	25.43688647115933	28.200366768889367	23.347613690650476
33	20.527885222912268	26.57803085173599	30.01687445100245	22.877209474349293
34	18.922886070487433	25.782465981414987	30.431801020172287	24.86284692792529
35	21.78807538795827	26.209720916614014	30.869072752770027	21.133130942657687
36	21.925998982909803	25.4072213404016	31.03396464841041	21.63281502827819
37	22.62062535636683	27.603982062227427	26.223590328396853	23.55180225300889
38	22.330908754680927	26.263657517991707	29.506017783668	21.899415943659367
39	22.237675486585196	24.709898136875683	30.088918339985515	22.963508036553606
40	23.875807122713475	25.869920327934537	27.706846866283456	22.547425683068532
41	21.478325191474934	23.61190303740118	30.07235209813379	24.83741967299009
42	25.198794902220644	25.356752091969607	27.259558336287004	22.18489466952274
43	21.654774930267678	25.03582931377233	29.02058837126874	24.288807384691253
44	24.355072352098134	23.425051239771307	26.859271701776827	25.360604706353733
45	24.229091861737377	25.146014085158185	27.820498990615032	22.804395062489405
46	21.02756930853277	24.506865358832503	31.489728930051935	22.975836402582793
47	21.410133916875992	25.655329706738993	28.016982324205202	24.91755405217981
48	21.927154767225037	24.2394939205745	31.640366152471067	22.192985159729393
49	24.319628299764222	23.085635912530243	28.443466736527405	24.15126905117813
50	20.865759504399687	24.006025488896764	28.12678183415266	27.001433172550893
51	21.71680202185203	23.30831702393244	30.429874712980226	24.5450062412353
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	2000.0
1	1615.5
2	1231.0
3	830.5
4	430.0
5	390.0
6	350.0
7	363.0
8	376.0
9	377.5
10	379.0
11	471.0
12	563.0
13	654.5
14	746.0
15	745.0
16	744.0
17	811.0
18	878.0
19	897.0
20	916.0
21	1102.0
22	1288.0
23	1260.5
24	1233.0
25	1712.5
26	2288.0
27	2384.0
28	2931.5
29	3479.0
30	3990.5
31	4502.0
32	5119.0
33	5736.0
34	6234.0
35	6732.0
36	7626.0
37	8520.0
38	9150.5
39	9781.0
40	10706.0
41	11631.0
42	13847.0
43	16063.0
44	19413.0
45	22763.0
46	24494.5
47	26226.0
48	27106.5
49	27987.0
50	26433.0
51	24879.0
52	22493.5
53	20108.0
54	17893.5
55	15679.0
56	13301.0
57	10923.0
58	9892.0
59	8861.0
60	7824.5
61	6788.0
62	5626.0
63	4464.0
64	3890.5
65	3317.0
66	2792.0
67	2267.0
68	1690.5
69	1114.0
70	920.5
71	727.0
72	631.0
73	535.0
74	421.0
75	287.0
76	267.0
77	187.0
78	107.0
79	71.5
80	36.0
81	25.0
82	14.0
83	11.0
84	8.0
85	4.5
86	1.0
87	9.5
88	18.0
89	11.0
90	4.0
91	5.5
92	7.0
93	4.0
94	1.0
95	1.0
96	1.0
97	1.0
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	259564.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	34.82763403245443
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.4391592920354	26.970226996039514
2	9.455752212389381	6.586429551093372
3	3.3451327433628317	3.4950917692746297
4	1.598451327433628	2.226811114021975
5	1.0254424778761062	1.7856867670401135
6	0.7289823008849557	1.5233237274814688
7	0.5752212389380531	1.4023516358200676
8	0.42477876106194695	1.183523138801991
9	0.37610619469026546	1.1789000015410456
>10	4.422566371681416	34.34181935861676
>50	0.538716814159292	12.454346519548166
>100	0.06305309734513274	3.792128338290364
>500	0.0011061946902654867	0.25619885654405083
>1k	0.0055309734513274336	2.803162225886487
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTGTCTCTTATACACATCTGACGCTTGCTTCGTCGTATGCCGTCTTCTGCT	1985	0.7647439552480313	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1696	0.6534033995469326	No Hit
AATGATACCTGTCTCTTATACACATCTGACGCTTGCTTCGTCGTATGCCGT	1240	0.477724183631012	No Hit
AATGATACGGCTGTCTCTTATACACATCTGACGCTTGCTTCGTCGTATGCC	1211	0.4665516019170609	No Hit
AATCTGTCTCTTATACACATCTGACGCTTGCTTCGTCGTATGCCGTCTTCT	1144	0.4407390855434498	No Hit
AAACTGTCTCTTATACACATCTGACGCTTGCTTCGTCGTATGCCGTCTTCT	665	0.25619885654405083	No Hit
AAAAACTGTCTCTTATACACATCTGACGCTTGCTTCGTCGTATGCCGTCTT	498	0.19186019632922902	No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	489	0.18839284338352005	No Hit
CCTGTCTCTTATACACATCTGACGCTTGCTTCGTCGTATGCCGTCTTCTGC	458	0.17644973879274475	No Hit
GCTCTAGGGATTTGGAGAAAGAAGATTAGAAAATGGAAGTGAAAGACGAAG	354	0.1363825491978857	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	320	0.12328366029187407	No Hit
AATGACTGTCTCTTATACACATCTGACGCTTGCTTCGTCGTATGCCGTCTT	308	0.11866052303092878	No Hit
AAAAAACTGTCTCTTATACACATCTGACGCTTGCTTCGTCGTATGCCGTCT	278	0.1071026798785656	No Hit
AATGATCTGTCTCTTATACACATCTGACGCTTGCTTCGTCGTATGCCGTCT	272	0.10479111124809297	No Hit
AACTGTCTCTTATACACATCTGACGCTTGCTTCGTCGTATGCCGTCTTCTG	268	0.10325006549444453	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	7.705228768242129E-4	0.0	0.0	0.8444930729993374	0.0
2	7.705228768242129E-4	0.0	0.0	1.251714413400934	0.0
3	7.705228768242129E-4	0.0	0.0	1.7124870937418133	0.0
4	7.705228768242129E-4	0.0	0.0	3.8410565409687014	0.0
5	7.705228768242129E-4	0.0	0.0	4.1847097440323004	0.0
6	7.705228768242129E-4	0.0	0.0	5.738854386586738	0.0
7	7.705228768242129E-4	0.0	0.0	6.909278636482717	0.0
8	7.705228768242129E-4	0.0	0.0	7.857792297853323	0.0
9	7.705228768242129E-4	0.0	0.0	9.465488280347044	0.0
10	7.705228768242129E-4	0.0	0.0	11.390639688092339	0.0
11	7.705228768242129E-4	0.0	0.0	13.279191259188485	0.0
12	7.705228768242129E-4	0.0	0.0	14.111355966158635	0.0
13	7.705228768242129E-4	0.0	0.0	14.533987764096716	0.0
14	7.705228768242129E-4	0.0	0.0	14.959701653542094	0.0
15	7.705228768242129E-4	0.0	0.0	15.316068484073293	0.0
16	7.705228768242129E-4	0.0	0.0	15.999137014377958	0.0
17	7.705228768242129E-4	0.0	0.0	16.944953845679677	0.0
18	7.705228768242129E-4	0.0	0.0	18.372347474996534	0.0
19	7.705228768242129E-4	0.0	0.0	18.87472839068592	0.0
20	7.705228768242129E-4	0.0	0.0	19.543542247769338	0.0
21	7.705228768242129E-4	0.0	0.0	20.071350418393923	0.0
22	7.705228768242129E-4	0.0	0.0	20.673899308070457	0.0
23	7.705228768242129E-4	0.0	0.0	21.23445470096007	0.0
24	7.705228768242129E-4	0.0	0.0	21.621642446564238	0.0
25	0.0011557843152363194	0.0	0.0	21.98378819867162	0.0
26	0.0011557843152363194	0.0	0.0	22.338613983449168	0.0
27	0.0011557843152363194	0.0	0.0	22.778967807554206	0.0
28	0.0011557843152363194	0.0	0.0	23.157294540074894	0.0
29	0.0011557843152363194	0.0	0.0	23.570294802052672	0.0
30	0.0011557843152363194	0.0	0.0	24.224083463038017	0.0
31	0.0011557843152363194	0.0	0.0	24.63246058775485	0.0
32	0.0011557843152363194	0.0	0.0	25.043149281102156	0.0
33	0.0011557843152363194	0.0	0.0	25.421476013622843	0.0
34	0.0011557843152363194	0.0	0.0	25.80249957621242	0.0
35	0.0011557843152363194	0.0	0.0	26.17812947866422	0.0
36	0.0011557843152363194	0.0	0.0	26.56146460988427	0.0
37	0.0011557843152363194	0.0	0.0	26.95520179994144	0.0
38	0.0011557843152363194	0.0	0.0	27.34662742136814	0.0
39	0.0011557843152363194	0.0	0.0	27.75307823889291	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGCCCA	20	7.023184E-4	45.000004	17
CGGGTCC	20	7.023184E-4	45.000004	6
CTAGAAT	20	7.023184E-4	45.000004	28
GCCCATT	20	7.023184E-4	45.000004	40
AGTCCGT	20	7.023184E-4	45.000004	37
ATCTACA	20	7.023184E-4	45.000004	10
GTCGAAC	20	7.023184E-4	45.000004	36
ATAGGCG	20	7.023184E-4	45.000004	1
CACGGAG	20	7.023184E-4	45.000004	22
GCGAATG	20	7.023184E-4	45.000004	1
GAGTCCG	20	7.023184E-4	45.000004	36
GGGTCGT	20	7.023184E-4	45.000004	8
GATCCGT	20	7.023184E-4	45.000004	30
ACTTCGC	20	7.023184E-4	45.000004	14
GTTAGGT	20	7.023184E-4	45.000004	18
TTCAACG	20	7.023184E-4	45.000004	1
TGAGTCC	20	7.023184E-4	45.000004	14
ACCTCGT	20	7.023184E-4	45.000004	10
ATCCGTT	20	7.023184E-4	45.000004	31
CGTTAGC	20	7.023184E-4	45.000004	32
>>END_MODULE