FastQCFastQC Report
Sat 18 Jun 2016
SRR3548424_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3548424_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences574128
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTCTCTTATACACATCTGACGCGACGATACTCGTATGCCGTCTTCTGCT39220.6831229272914751TruSeq Adapter, Index 23 (95% over 22bp)
AATGATACGGCTGTCTCTTATACACATCTGACGCGACGATACTCGTATGCC38080.6632667279770365No Hit
AATGATACCTGTCTCTTATACACATCTGACGCGACGATACTCGTATGCCGT37140.646894072401973No Hit
AATCTGTCTCTTATACACATCTGACGCGACGATACTCGTATGCCGTCTTCT32320.5629406682830309No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA18680.3253629852576429No Hit
AAACTGTCTCTTATACACATCTGACGCGACGATACTCGTATGCCGTCTTCT10430.18166680600841625No Hit
TGCAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGG9810.17086782041635315No Hit
AATGACTGTCTCTTATACACATCTGACGCGACGATACTCGTATGCCGTCTT8530.14857314048435194No Hit
AATGATCTGTCTCTTATACACATCTGACGCGACGATACTCGTATGCCGTCT8010.1395159267619764No Hit
AAAAACTGTCTCTTATACACATCTGACGCGACGATACTCGTATGCCGTCTT7170.12488504305660061No Hit
AACTGTCTCTTATACACATCTGACGCGACGATACTCGTATGCCGTCTTCTG7020.12227238525206922No Hit
CCTGTCTCTTATACACATCTGACGCGACGATACTCGTATGCCGTCTTCTGC6680.1163503608951314TruSeq Adapter, Index 20 (95% over 21bp)
TGTATGGGGTCAGAGATTGTATTTGTTTGTTTAATTTTTTACTGTTTGAGG5930.10328707187247443No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTTAGCG351.210501E-745.0000041
TAAACGT351.210501E-745.00000418
TAACGGT351.210501E-745.00000419
CCCGTTC351.210501E-745.00000416
CCCCGTT351.210501E-745.00000415
CGGTCTA351.210501E-745.00000431
CCGTTCG351.210501E-745.00000417
TATCACG453.8380676E-1045.01
CAGAACG750.045.01
CTCGTCA502.1827873E-1145.036
AATCACG453.8380676E-1045.01
ATCTACG207.0303347E-445.01
GGTCGAT207.0303347E-445.015
CGACGTA207.0303347E-445.03
CATGCGT207.0303347E-445.013
ACGTTCA207.0303347E-445.031
CCGATCG207.0303347E-445.026
TTTCGCT207.0303347E-445.015
CGTGACC207.0303347E-445.039
GACCGGT207.0303347E-445.021