Basic Statistics
Measure | Value |
---|---|
Filename | SRR3548424_1.fastq |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 574128 |
Sequences flagged as poor quality | 0 |
Sequence length | 51 |
%GC | 49 |
Per base sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
CTGTCTCTTATACACATCTGACGCGACGATACTCGTATGCCGTCTTCTGCT | 3922 | 0.6831229272914751 | TruSeq Adapter, Index 23 (95% over 22bp) |
AATGATACGGCTGTCTCTTATACACATCTGACGCGACGATACTCGTATGCC | 3808 | 0.6632667279770365 | No Hit |
AATGATACCTGTCTCTTATACACATCTGACGCGACGATACTCGTATGCCGT | 3714 | 0.646894072401973 | No Hit |
AATCTGTCTCTTATACACATCTGACGCGACGATACTCGTATGCCGTCTTCT | 3232 | 0.5629406682830309 | No Hit |
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA | 1868 | 0.3253629852576429 | No Hit |
AAACTGTCTCTTATACACATCTGACGCGACGATACTCGTATGCCGTCTTCT | 1043 | 0.18166680600841625 | No Hit |
TGCAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGG | 981 | 0.17086782041635315 | No Hit |
AATGACTGTCTCTTATACACATCTGACGCGACGATACTCGTATGCCGTCTT | 853 | 0.14857314048435194 | No Hit |
AATGATCTGTCTCTTATACACATCTGACGCGACGATACTCGTATGCCGTCT | 801 | 0.1395159267619764 | No Hit |
AAAAACTGTCTCTTATACACATCTGACGCGACGATACTCGTATGCCGTCTT | 717 | 0.12488504305660061 | No Hit |
AACTGTCTCTTATACACATCTGACGCGACGATACTCGTATGCCGTCTTCTG | 702 | 0.12227238525206922 | No Hit |
CCTGTCTCTTATACACATCTGACGCGACGATACTCGTATGCCGTCTTCTGC | 668 | 0.1163503608951314 | TruSeq Adapter, Index 20 (95% over 21bp) |
TGTATGGGGTCAGAGATTGTATTTGTTTGTTTAATTTTTTACTGTTTGAGG | 593 | 0.10328707187247443 | No Hit |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
TTTAGCG | 35 | 1.210501E-7 | 45.000004 | 1 |
TAAACGT | 35 | 1.210501E-7 | 45.000004 | 18 |
TAACGGT | 35 | 1.210501E-7 | 45.000004 | 19 |
CCCGTTC | 35 | 1.210501E-7 | 45.000004 | 16 |
CCCCGTT | 35 | 1.210501E-7 | 45.000004 | 15 |
CGGTCTA | 35 | 1.210501E-7 | 45.000004 | 31 |
CCGTTCG | 35 | 1.210501E-7 | 45.000004 | 17 |
TATCACG | 45 | 3.8380676E-10 | 45.0 | 1 |
CAGAACG | 75 | 0.0 | 45.0 | 1 |
CTCGTCA | 50 | 2.1827873E-11 | 45.0 | 36 |
AATCACG | 45 | 3.8380676E-10 | 45.0 | 1 |
ATCTACG | 20 | 7.0303347E-4 | 45.0 | 1 |
GGTCGAT | 20 | 7.0303347E-4 | 45.0 | 15 |
CGACGTA | 20 | 7.0303347E-4 | 45.0 | 3 |
CATGCGT | 20 | 7.0303347E-4 | 45.0 | 13 |
ACGTTCA | 20 | 7.0303347E-4 | 45.0 | 31 |
CCGATCG | 20 | 7.0303347E-4 | 45.0 | 26 |
TTTCGCT | 20 | 7.0303347E-4 | 45.0 | 15 |
CGTGACC | 20 | 7.0303347E-4 | 45.0 | 39 |
GACCGGT | 20 | 7.0303347E-4 | 45.0 | 21 |