##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3548424_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	574128
Sequences flagged as poor quality	0
Sequence length	51
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.767036270657414	31.0	31.0	34.0	30.0	34.0
2	31.966638101608005	33.0	31.0	34.0	30.0	34.0
3	32.12948506256445	34.0	31.0	34.0	30.0	34.0
4	35.78534229300783	37.0	35.0	37.0	35.0	37.0
5	35.55389216342	37.0	35.0	37.0	33.0	37.0
6	34.86141243764457	37.0	35.0	37.0	32.0	37.0
7	35.27342508987543	37.0	35.0	37.0	33.0	37.0
8	35.71033288743973	37.0	35.0	37.0	33.0	37.0
9	37.40629441518268	39.0	37.0	39.0	35.0	39.0
10	36.93053117074938	39.0	37.0	39.0	33.0	39.0
11	36.951320959785974	39.0	37.0	39.0	33.0	39.0
12	36.743496223838584	39.0	35.0	39.0	33.0	39.0
13	36.722676824680214	39.0	35.0	39.0	33.0	39.0
14	37.82879253406906	40.0	37.0	41.0	33.0	41.0
15	37.87915064236547	40.0	37.0	41.0	33.0	41.0
16	37.72323419167851	40.0	36.0	41.0	33.0	41.0
17	37.717068319259816	39.0	36.0	41.0	33.0	41.0
18	37.66166081431319	39.0	36.0	41.0	33.0	41.0
19	37.69136499177883	40.0	36.0	41.0	32.0	41.0
20	37.63823224089402	39.0	36.0	41.0	33.0	41.0
21	37.65236149430092	39.0	36.0	41.0	33.0	41.0
22	37.645216746091464	39.0	36.0	41.0	33.0	41.0
23	37.56771138143411	39.0	36.0	41.0	33.0	41.0
24	37.40533469888248	39.0	36.0	41.0	32.0	41.0
25	37.48632883259482	39.0	36.0	41.0	32.0	41.0
26	37.427700791461135	39.0	36.0	41.0	32.0	41.0
27	37.327524524147925	39.0	35.0	41.0	32.0	41.0
28	37.29189832232534	39.0	35.0	41.0	32.0	41.0
29	37.0305802887161	39.0	35.0	41.0	31.0	41.0
30	37.13774802831424	39.0	35.0	41.0	32.0	41.0
31	37.01622983028175	39.0	35.0	41.0	31.0	41.0
32	36.9449756848647	39.0	35.0	41.0	31.0	41.0
33	36.76792457431095	39.0	35.0	41.0	31.0	41.0
34	36.64236198199705	39.0	35.0	41.0	30.0	41.0
35	36.630113842209404	39.0	35.0	41.0	30.0	41.0
36	36.61983564640638	39.0	35.0	40.0	31.0	41.0
37	36.594367458127806	39.0	35.0	40.0	31.0	41.0
38	36.4528763620656	39.0	35.0	40.0	30.0	41.0
39	36.36447621436335	39.0	35.0	40.0	30.0	41.0
40	36.3061738845693	39.0	35.0	40.0	30.0	41.0
41	36.2947966307165	39.0	35.0	40.0	30.0	41.0
42	36.26254424100549	38.0	35.0	40.0	30.0	41.0
43	36.17672191567037	38.0	35.0	40.0	30.0	41.0
44	36.11667433046289	38.0	35.0	40.0	30.0	41.0
45	35.86066695928434	38.0	35.0	40.0	29.0	41.0
46	35.641818549173706	38.0	34.0	40.0	28.0	41.0
47	35.53549731070423	38.0	34.0	40.0	28.0	41.0
48	35.45148816988546	37.0	34.0	40.0	28.0	41.0
49	35.33632221386172	37.0	34.0	40.0	28.0	41.0
50	35.239291586545164	37.0	34.0	40.0	28.0	41.0
51	33.95595233118747	36.0	32.0	39.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	1.0
10	6.0
11	6.0
12	7.0
13	6.0
14	18.0
15	25.0
16	46.0
17	105.0
18	230.0
19	473.0
20	798.0
21	1177.0
22	1689.0
23	2282.0
24	2846.0
25	3280.0
26	3994.0
27	5141.0
28	6364.0
29	8184.0
30	10715.0
31	14096.0
32	19077.0
33	26177.0
34	41062.0
35	48786.0
36	53549.0
37	76252.0
38	113552.0
39	134131.0
40	52.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	19.353001421285846	33.10968982526545	20.347204804503523	27.190103948945183
2	23.19883370955606	32.12454365577015	20.70566145528523	23.97096117938857
3	20.6751804475657	30.307875595685978	22.835500097539224	26.181443859209097
4	22.724723406627092	31.81834016107906	18.2783281776886	27.178608254605248
5	17.63369840871722	35.039224702505365	17.991109996377112	29.3359668924003
6	20.389355683749965	38.026711813393526	20.263251400384583	21.320681102471923
7	81.88417913775325	7.226088955772929	6.033671933784801	4.856059972689017
8	83.86561881670987	4.720724298414291	4.624230136833598	6.789426748042249
9	77.91224256611767	7.732422038291113	8.985278544157401	5.3700568514338265
10	34.88612295515983	33.46396622355991	13.728645876877628	17.921264944402644
11	27.635997547585205	26.749435665914223	25.783100632611543	19.83146615388903
12	27.3439024050386	23.024133991026392	30.651178831201403	18.980784772733607
13	27.20577989577237	24.182238107181675	25.746175068974164	22.865806928071787
14	20.816438146197363	26.625247331605493	30.199537385391412	22.35877713680573
15	20.697126773123763	29.09629908313129	28.166889613465983	22.039684530278965
16	22.706086447621438	27.233299891313433	27.245666471588216	22.81494718947691
17	21.345588440208456	27.630249700415238	28.2515397263328	22.7726221330435
18	23.360818493437	26.19555221135357	27.054594097483488	23.389035197725942
19	23.30926204609425	30.205633586935317	26.391153192319482	20.093951174650947
20	25.08099239194047	29.177639829445702	25.16877769417273	20.5725900844411
21	24.403791489005936	28.303792882423433	25.43997157428309	21.852444054287545
22	21.48980715101859	27.270225454950815	27.30000975392247	23.93995764010813
23	23.510262519856198	27.298093804865815	25.265097678566452	23.926545996711535
24	20.6835409525402	29.072262631329597	26.717909595072875	23.526286821057322
25	24.12667558453864	26.330713708441323	23.84955968007134	25.693051026948694
26	23.589164785553045	27.41653429200457	23.736867040102556	25.257433882339825
27	19.925347657665192	26.07554412953209	26.79106401359975	27.208044199202963
28	22.993478806119892	26.948694367806482	26.15165956023744	23.90616726583619
29	22.25026474932419	31.978583173090318	22.44325307248558	23.32789900509991
30	24.501156536521474	24.97038987821531	25.748961903965668	24.77949168129755
31	26.08982665886353	28.624278906445948	22.85709806872335	22.42879636596717
32	24.015376362065602	27.10336370983474	24.43984616670847	24.441413761391185
33	25.075070367583535	25.232178190229355	26.034612490594434	23.658138951592676
34	22.166485522392218	27.931402056684224	26.208789677563193	23.693322743360365
35	22.91771172978848	26.127448931248782	27.002689295766796	23.952150043195942
36	22.65191734247415	30.64194744029206	25.841798344620017	20.864336872613773
37	22.318890560989885	30.785643619541286	22.206197921021094	24.689267898447735
38	24.206274558983363	28.021277485160102	26.311380040687794	21.461067915168744
39	22.278133099239195	27.87044004124516	25.529672825571996	24.321754033943648
40	23.15755371624446	26.038966920268653	25.538555862107405	25.26492350137948
41	19.975859041886128	26.044192235877713	28.186919989967397	25.793028732268763
42	23.615291363598363	26.871185519605383	23.307868628598502	26.205654488197755
43	21.355516539865675	25.323621213387952	26.664959730234372	26.655902516511997
44	22.79596187609732	22.706086447621438	25.928886938104394	28.569064738176852
45	25.069496697600535	25.11913719588663	26.339596744976728	23.471769361536104
46	20.67483209319176	25.22677869743333	30.244997631190255	23.853391578184656
47	21.514888665942088	26.232129420617007	27.12844522475824	25.124536688682664
48	21.23097985118301	24.40466237494078	29.95133489396093	24.41302287991528
49	21.682969651366943	24.00562243959535	29.201850458434357	25.109557450603347
50	21.958169606777584	23.18716383802915	27.316034055123595	27.53863250006967
51	21.369276537636207	22.252354875567818	30.895026892957667	25.48334169383831
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	2241.0
1	1826.0
2	1411.0
3	950.5
4	490.0
5	440.0
6	390.0
7	461.0
8	532.0
9	564.5
10	597.0
11	674.0
12	751.0
13	849.5
14	948.0
15	959.5
16	971.0
17	1030.5
18	1090.0
19	1201.5
20	1313.0
21	1361.0
22	1409.0
23	1845.5
24	2282.0
25	2682.5
26	4133.5
27	5184.0
28	6544.5
29	7905.0
30	8590.5
31	9276.0
32	10236.5
33	11197.0
34	11298.5
35	11400.0
36	13367.5
37	15335.0
38	16785.0
39	18235.0
40	20197.5
41	22160.0
42	24483.5
43	26807.0
44	35671.5
45	44536.0
46	48341.0
47	52146.0
48	53573.0
49	55000.0
50	51510.5
51	48021.0
52	45448.5
53	42876.0
54	38032.0
55	33188.0
56	32830.0
57	32472.0
58	29103.0
59	25734.0
60	26361.5
61	26989.0
62	24269.0
63	21549.0
64	19404.0
65	17259.0
66	13894.5
67	10530.0
68	8673.5
69	6817.0
70	5721.0
71	4625.0
72	3767.5
73	2910.0
74	2424.0
75	1555.5
76	1173.0
77	903.5
78	634.0
79	556.5
80	479.0
81	297.5
82	116.0
83	83.5
84	51.0
85	37.0
86	23.0
87	33.0
88	43.0
89	23.0
90	3.0
91	3.5
92	4.0
93	4.0
94	4.0
95	2.0
96	0.0
97	0.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	574128.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	25.389038719773744
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.96496789155067	19.03288472425175
2	10.491074218909494	5.327165791118264
3	3.9924868647238396	3.040962107899849
4	1.8318309818810368	1.8603371090823515
5	1.1335092510318376	1.4389355131834531
6	0.694781796508908	1.0583905160015177
7	0.5290633868092043	0.9402687569039465
8	0.41610708747654485	0.8451647164411422
9	0.3055322812977249	0.6981453828007868
>10	3.9314725181369004	24.14733623893707
>50	1.2939388788622839	23.5003530604734
>100	0.40474911445773215	13.84712291955392
>500	0.006291437012296355	1.1415623509173394
>1k	0.0041942913415309035	3.1213708124352064
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTGTCTCTTATACACATCTGACGCGACGATACTCGTATGCCGTCTTCTGCT	3922	0.6831229272914751	TruSeq Adapter, Index 23 (95% over 22bp)
AATGATACGGCTGTCTCTTATACACATCTGACGCGACGATACTCGTATGCC	3808	0.6632667279770365	No Hit
AATGATACCTGTCTCTTATACACATCTGACGCGACGATACTCGTATGCCGT	3714	0.646894072401973	No Hit
AATCTGTCTCTTATACACATCTGACGCGACGATACTCGTATGCCGTCTTCT	3232	0.5629406682830309	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1868	0.3253629852576429	No Hit
AAACTGTCTCTTATACACATCTGACGCGACGATACTCGTATGCCGTCTTCT	1043	0.18166680600841625	No Hit
TGCAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGG	981	0.17086782041635315	No Hit
AATGACTGTCTCTTATACACATCTGACGCGACGATACTCGTATGCCGTCTT	853	0.14857314048435194	No Hit
AATGATCTGTCTCTTATACACATCTGACGCGACGATACTCGTATGCCGTCT	801	0.1395159267619764	No Hit
AAAAACTGTCTCTTATACACATCTGACGCGACGATACTCGTATGCCGTCTT	717	0.12488504305660061	No Hit
AACTGTCTCTTATACACATCTGACGCGACGATACTCGTATGCCGTCTTCTG	702	0.12227238525206922	No Hit
CCTGTCTCTTATACACATCTGACGCGACGATACTCGTATGCCGTCTTCTGC	668	0.1163503608951314	TruSeq Adapter, Index 20 (95% over 21bp)
TGTATGGGGTCAGAGATTGTATTTGTTTGTTTAATTTTTTACTGTTTGAGG	593	0.10328707187247443	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.7604575983056043	0.0
2	0.0	0.0	0.0	1.0391410974556197	0.0
3	0.0	0.0	0.0	1.4707521667642058	0.0
4	0.0	0.0	0.0	3.3762505922024357	0.0
5	0.0	0.0	0.0	3.647792826686732	0.0
6	0.0	0.0	0.0	4.8962949028788	0.0
7	0.0	0.0	0.0	5.918889167572388	0.0
8	0.0	0.0	0.0	6.643814619736365	0.0
9	0.0	0.0	0.0	8.231788033330547	0.0
10	0.0	0.0	0.0	9.71839032410891	0.0
11	0.0	0.0	0.0	11.592362678705793	0.0
12	0.0	0.0	0.0	12.259112950422205	0.0
13	0.0	0.0	0.0	12.556955940138785	0.0
14	0.0	0.0	0.0	12.965053089206588	0.0
15	0.0	0.0	0.0	13.277526962628542	0.0
16	0.0	0.0	0.0	13.877915726109856	0.0
17	0.0	0.0	0.0	14.573231056489146	0.0
18	0.0	0.0	0.0	15.689532647771925	0.0
19	0.0	0.0	0.0	16.096933088091856	0.0
20	0.0	0.0	0.0	16.579578073182287	0.0
21	0.0	0.0	0.0	17.030522810244406	0.0
22	0.0	0.0	0.0	17.51403868126968	0.0
23	0.0	0.0	0.0	18.003476576651895	0.0
24	0.0	0.0	0.0	18.38074436362623	0.0
25	0.0	0.0	0.0	18.69286988267425	0.0
26	0.0	0.0	0.0	18.98879692333417	0.0
27	0.0	0.0	0.0	19.33453863946716	0.0
28	0.0	0.0	0.0	19.68689908870496	0.0
29	0.0	0.0	0.0	20.032640804837946	0.0
30	0.0	0.0	0.0	20.503267564027535	0.0
31	0.0	0.0	0.0	20.810516121840426	0.0
32	0.0	0.0	0.0	21.164618342947914	0.0
33	0.0	0.0	0.0	21.505657275032746	0.0
34	0.0	0.0	0.0	21.853489117409357	0.0
35	0.0	0.0	0.0	22.210552350695316	0.0
36	0.0	0.0	0.0	22.54706267591896	0.0
37	0.0	0.0	0.0	22.87556085054204	0.0
38	0.0	0.0	0.0	23.258228130312403	0.0
39	0.0	0.0	0.0	23.676775910598334	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTTAGCG	35	1.210501E-7	45.000004	1
TAAACGT	35	1.210501E-7	45.000004	18
TAACGGT	35	1.210501E-7	45.000004	19
CCCGTTC	35	1.210501E-7	45.000004	16
CCCCGTT	35	1.210501E-7	45.000004	15
CGGTCTA	35	1.210501E-7	45.000004	31
CCGTTCG	35	1.210501E-7	45.000004	17
TATCACG	45	3.8380676E-10	45.0	1
CAGAACG	75	0.0	45.0	1
CTCGTCA	50	2.1827873E-11	45.0	36
AATCACG	45	3.8380676E-10	45.0	1
ATCTACG	20	7.0303347E-4	45.0	1
GGTCGAT	20	7.0303347E-4	45.0	15
CGACGTA	20	7.0303347E-4	45.0	3
CATGCGT	20	7.0303347E-4	45.0	13
ACGTTCA	20	7.0303347E-4	45.0	31
CCGATCG	20	7.0303347E-4	45.0	26
TTTCGCT	20	7.0303347E-4	45.0	15
CGTGACC	20	7.0303347E-4	45.0	39
GACCGGT	20	7.0303347E-4	45.0	21
>>END_MODULE