SRR3548423_1.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR3548423_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	584891
Sequences flagged as poor quality	0
Sequence length	51
%GC	49

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
AATGATACGGCTGTCTCTTATACACATCTGACGCGACGATACTCGTATGCC	4539	0.7760420317631832	No Hit
CTGTCTCTTATACACATCTGACGCGACGATACTCGTATGCCGTCTTCTGCT	4149	0.7093629411292018	TruSeq Adapter, Index 23 (95% over 22bp)
AATGATACCTGTCTCTTATACACATCTGACGCGACGATACTCGTATGCCGT	4106	0.7020111439567372	No Hit
AATCTGTCTCTTATACACATCTGACGCGACGATACTCGTATGCCGTCTTCT	3663	0.626270535877625	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1921	0.3284372643791749	No Hit
AAACTGTCTCTTATACACATCTGACGCGACGATACTCGTATGCCGTCTTCT	1153	0.1971307474384116	No Hit
AATGACTGTCTCTTATACACATCTGACGCGACGATACTCGTATGCCGTCTT	954	0.16310731401235443	No Hit
TGCAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGG	923	0.15780718116708925	No Hit
AATGATCTGTCTCTTATACACATCTGACGCGACGATACTCGTATGCCGTCT	840	0.1436165029039599	No Hit
AAAAACTGTCTCTTATACACATCTGACGCGACGATACTCGTATGCCGTCTT	810	0.13848734208596133	No Hit
AACTGTCTCTTATACACATCTGACGCGACGATACTCGTATGCCGTCTTCTG	739	0.12634832815003136	No Hit
CCTGTCTCTTATACACATCTGACGCGACGATACTCGTATGCCGTCTTCTGC	728	0.12446763585009857	TruSeq Adapter, Index 20 (95% over 21bp)
AATGATACGGCGACTGTCTCTTATACACATCTGACGCGACGATACTCGTAT	640	0.1094220974506361	No Hit
AATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGACGATACTC	587	0.1003605800055053	No Hit

Adapter graph

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGCGATT	35	1.2105556E-7	45.000004	11
TCTAGCG	70	0.0	45.000004	1
TAGCACA	35	1.2105556E-7	45.000004	11
TGATTCG	35	1.2105556E-7	45.000004	15
TAACACG	35	1.2105556E-7	45.000004	1
TATCGGT	35	1.2105556E-7	45.000004	29
CTTCGTC	25	3.888448E-5	45.0	41
AGGTATC	20	7.030441E-4	45.0	33
AGCGTAA	20	7.030441E-4	45.0	1
AGCCGAT	25	3.888448E-5	45.0	12
TCGTTAT	25	3.888448E-5	45.0	43
TCGCACG	20	7.030441E-4	45.0	1
ACGCCTA	25	3.888448E-5	45.0	43
CTCGTCA	50	2.1827873E-11	45.0	36
CGTATAG	20	7.030441E-4	45.0	1
AATCACC	20	7.030441E-4	45.0	23
ACGTTTG	20	7.030441E-4	45.0	45
CCGATTC	20	7.030441E-4	45.0	24
CGAAACG	25	3.888448E-5	45.0	1
ACGTTGT	20	7.030441E-4	45.0	11