##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR3548423_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 584891 Sequences flagged as poor quality 0 Sequence length 51 %GC 49 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.77866987182227 31.0 31.0 34.0 30.0 34.0 2 31.983559329858043 33.0 31.0 34.0 30.0 34.0 3 32.12274081837471 34.0 31.0 34.0 30.0 34.0 4 35.77495635939004 37.0 35.0 37.0 35.0 37.0 5 35.55672766378693 37.0 35.0 37.0 33.0 37.0 6 35.58494317744674 37.0 35.0 37.0 33.0 37.0 7 35.64305998895521 37.0 35.0 37.0 35.0 37.0 8 35.727627882802096 37.0 35.0 37.0 35.0 37.0 9 37.43948872524966 39.0 37.0 39.0 35.0 39.0 10 36.95529936347114 39.0 37.0 39.0 33.0 39.0 11 36.96755976754643 39.0 37.0 39.0 33.0 39.0 12 36.780076971606675 39.0 35.0 39.0 33.0 39.0 13 36.767001030961325 39.0 35.0 39.0 33.0 39.0 14 37.8717795281514 40.0 37.0 41.0 33.0 41.0 15 37.91542355755175 40.0 37.0 41.0 33.0 41.0 16 37.772027608562965 40.0 36.0 41.0 33.0 41.0 17 37.736350875633235 40.0 36.0 41.0 33.0 41.0 18 37.65503145030441 39.0 36.0 41.0 33.0 41.0 19 37.70110157277168 40.0 36.0 41.0 32.0 41.0 20 37.65847995609438 39.0 36.0 41.0 33.0 41.0 21 37.65510838771669 39.0 36.0 41.0 33.0 41.0 22 37.639042146314445 39.0 36.0 41.0 33.0 41.0 23 37.52398994000592 39.0 36.0 41.0 32.0 41.0 24 37.39247654691216 39.0 36.0 41.0 32.0 41.0 25 37.461557794529234 39.0 36.0 41.0 32.0 41.0 26 37.39621228570793 39.0 36.0 41.0 32.0 41.0 27 37.33476835854886 39.0 36.0 41.0 32.0 41.0 28 37.27480846858646 39.0 35.0 41.0 32.0 41.0 29 37.05448536564933 39.0 35.0 41.0 31.0 41.0 30 37.13261616266963 39.0 35.0 41.0 31.0 41.0 31 36.984992075446534 39.0 35.0 41.0 31.0 41.0 32 36.89575664525527 39.0 35.0 41.0 31.0 41.0 33 36.872825876958274 39.0 35.0 41.0 31.0 41.0 34 36.70040229718016 39.0 35.0 41.0 31.0 41.0 35 36.66715678647817 39.0 35.0 41.0 30.0 41.0 36 36.614546983967955 39.0 35.0 40.0 31.0 41.0 37 36.588480588690885 39.0 35.0 40.0 31.0 41.0 38 36.54301570720015 39.0 35.0 40.0 31.0 41.0 39 36.406299635316664 39.0 35.0 40.0 30.0 41.0 40 36.31150590451896 39.0 35.0 40.0 30.0 41.0 41 36.26449030674092 38.0 35.0 40.0 30.0 41.0 42 36.224585435576884 38.0 35.0 40.0 30.0 41.0 43 36.124195790326745 38.0 35.0 40.0 30.0 41.0 44 36.07867448806701 38.0 35.0 40.0 30.0 41.0 45 35.84073955660114 38.0 35.0 40.0 29.0 41.0 46 35.625347286930385 38.0 34.0 40.0 28.0 41.0 47 35.48354479723572 38.0 34.0 40.0 28.0 41.0 48 35.41461400500264 37.0 34.0 40.0 28.0 41.0 49 35.24178864096045 37.0 34.0 40.0 28.0 41.0 50 35.14728043344828 37.0 34.0 40.0 28.0 41.0 51 33.787580933883405 36.0 32.0 39.0 24.0 41.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 7 2.0 8 3.0 9 0.0 10 6.0 11 4.0 12 8.0 13 13.0 14 16.0 15 27.0 16 42.0 17 117.0 18 221.0 19 422.0 20 750.0 21 1166.0 22 1653.0 23 2201.0 24 2837.0 25 3380.0 26 4148.0 27 5195.0 28 6539.0 29 8531.0 30 11090.0 31 14356.0 32 19281.0 33 26212.0 34 41297.0 35 49313.0 36 53871.0 37 77211.0 38 117541.0 39 137394.0 40 44.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 19.353007654417663 33.41340523276987 20.150763133643704 27.08282397916877 2 22.894522227218406 32.3514979714169 20.715141795650812 24.038838005713885 3 20.663508243416295 30.228880252901824 23.05814245731256 26.04946904636932 4 22.956072157034388 31.634612261087963 18.2582737638295 27.15104181804815 5 17.58994410924429 35.06362723994727 18.07225619816342 29.274172452645026 6 20.902527137535028 36.60630784197397 20.782162830339328 21.70900219015167 7 81.46988071281658 7.274859760194635 6.265098967157984 4.990160559830806 8 83.25055437679842 4.735412239203543 4.794568560637794 7.21946482336025 9 77.54607268704767 7.70998356958818 9.3022460595222 5.441697683841946 10 34.570372941283075 33.443838253623326 13.954907837528701 18.030880967564897 11 27.6778066340566 26.43654971610095 26.086740948313448 19.798902701529002 12 27.044184301006513 22.896060975463804 31.057581669063122 19.00217305446656 13 27.15719681102975 24.04875438329535 25.83472818012245 22.959320625552454 14 20.6419657679807 26.584440519686574 30.573730831898594 22.199862880434132 15 20.65000145326223 28.954967677738246 28.247656400936243 22.14737446806328 16 22.552749144712433 27.131038090857956 27.458107579018996 22.858105185410615 17 20.98425176656847 27.742092116308854 28.47846863774618 22.7951874793765 18 23.187397309926123 26.06297583652339 27.133944615321486 23.615682238229002 19 22.916235674681264 30.351125252397456 26.668387785074483 20.064251287846798 20 24.995597470297884 29.294518123889752 25.21512555330822 20.49475885250414 21 24.189464361735777 28.38272430247687 25.48902274098935 21.938788594798005 22 21.281914066039658 27.383392803103483 27.44135232034687 23.89334081050999 23 23.58302658102108 27.17275526551101 25.194950854090763 24.04926729937715 24 20.704712501987547 29.033443838253625 26.736947568008397 23.524896091750428 25 24.083803648885006 26.176159318573887 23.91967050270905 25.820366529832057 26 23.58712990967548 27.551629277933838 23.591233238329874 25.27000757406081 27 19.78881535192027 26.024678102415667 26.74447033720813 27.44203620845593 28 23.23048226079731 26.794565141197246 26.02775559890646 23.947196999098978 29 22.34399229941989 31.868501994388698 22.500089760314314 23.287415945877097 30 24.580648360121803 24.83129335209466 25.55912127216866 25.028937015614876 31 26.297036541851387 28.722787664710175 22.662342214190335 22.3178335792481 32 23.98310112482497 27.201136622037268 24.240072081806694 24.57569017133107 33 25.1927282177363 25.154943399710373 26.10777050766724 23.54455787488609 34 22.141048503054414 28.061467863242896 26.04263016527866 23.75485346842403 35 22.987531010051445 26.055453067323654 26.7348959036812 24.222120018943702 36 22.65259680863614 30.61835453101518 25.939705004864155 20.789343655484526 37 22.27577446054051 30.71358595020269 22.051630132793974 24.959009456462827 38 24.251527207633558 27.88519570313101 26.373290066012302 21.48998702322313 39 22.26893557944985 27.86553391999535 25.633152159975108 24.232378340579697 40 23.045148583240298 26.055965983405454 25.57587653084079 25.32300890251346 41 19.90969257519777 26.03288475972446 28.337074771196686 25.720347893881083 42 23.791270510231822 26.750283386135195 23.20312673643465 26.255319367198332 43 21.177450157379752 25.19871223869063 26.783793903479452 26.840043700450167 44 22.72628575238805 22.543174711185504 26.025532962551996 28.70500657387445 45 25.235813168607486 25.03799853306001 26.221466905799538 23.504721392532968 46 20.54194713202973 25.044837414150674 30.57185013959866 23.84136531422094 47 21.61240299474603 26.051520710696526 27.021786965434586 25.314289329122865 48 21.241393695577468 24.205535732298838 30.27350395201841 24.27956662010528 49 21.741999791414127 23.813496873776483 29.20202225713851 25.24248107767088 50 21.917588063416947 22.944446059180258 27.346462845213892 27.791503032188903 51 21.112993703100237 22.058981929966436 31.180681528695093 25.647342838238234 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 2287.0 1 1835.5 2 1384.0 3 953.5 4 523.0 5 467.5 6 412.0 7 485.0 8 558.0 9 570.0 10 582.0 11 675.5 12 769.0 13 883.5 14 998.0 15 991.5 16 985.0 17 1034.0 18 1083.0 19 1175.0 20 1267.0 21 1373.5 22 1480.0 23 1863.0 24 2246.0 25 2575.0 26 4002.5 27 5101.0 28 6417.0 29 7733.0 30 8370.5 31 9008.0 32 9957.0 33 10906.0 34 11286.0 35 11666.0 36 13651.0 37 15636.0 38 16960.5 39 18285.0 40 20360.0 41 22435.0 42 24762.0 43 27089.0 44 37029.0 45 46969.0 46 51300.5 47 55632.0 48 56090.0 49 56548.0 50 52937.0 51 49326.0 52 46363.5 53 43401.0 54 38583.0 55 33765.0 56 32992.5 57 32220.0 58 29076.5 59 25933.0 60 26524.5 61 27116.0 62 24438.5 63 21761.0 64 19431.0 65 17101.0 66 13835.0 67 10569.0 68 8803.0 69 7037.0 70 5849.5 71 4662.0 72 3830.5 73 2999.0 74 2484.5 75 1580.0 76 1190.0 77 902.0 78 614.0 79 553.5 80 493.0 81 305.0 82 117.0 83 84.0 84 51.0 85 36.5 86 22.0 87 32.0 88 42.0 89 24.5 90 7.0 91 5.0 92 3.0 93 4.0 94 5.0 95 3.0 96 1.0 97 0.5 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 44 0.0 45 0.0 46 0.0 47 0.0 48 0.0 49 0.0 50 0.0 51 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 51 584891.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 25.22647440791766 #Duplication Level Percentage of deduplicated Percentage of total 1 74.80555173735462 18.870803364725365 2 10.531152116965776 5.313276787290501 3 3.8973000146471155 2.949454172384178 4 2.0141708721383518 2.032417198366853 5 1.108403027757069 1.3980550306686073 6 0.7668535373215559 1.1607006680318015 7 0.5122770578829285 0.9046060863312935 8 0.4114743934747873 0.8304038605204132 9 0.34924395466884334 0.7929174316116179 >10 3.900077742154283 23.782792681872365 >50 1.2772806873393867 23.14290180921141 >100 0.41598570169622306 14.292111228944716 >500 0.006137493959452471 1.1696344954271853 >1k 0.0040916626396349815 3.359925184613672 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source AATGATACGGCTGTCTCTTATACACATCTGACGCGACGATACTCGTATGCC 4539 0.7760420317631832 No Hit CTGTCTCTTATACACATCTGACGCGACGATACTCGTATGCCGTCTTCTGCT 4149 0.7093629411292018 TruSeq Adapter, Index 23 (95% over 22bp) AATGATACCTGTCTCTTATACACATCTGACGCGACGATACTCGTATGCCGT 4106 0.7020111439567372 No Hit AATCTGTCTCTTATACACATCTGACGCGACGATACTCGTATGCCGTCTTCT 3663 0.626270535877625 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1921 0.3284372643791749 No Hit AAACTGTCTCTTATACACATCTGACGCGACGATACTCGTATGCCGTCTTCT 1153 0.1971307474384116 No Hit AATGACTGTCTCTTATACACATCTGACGCGACGATACTCGTATGCCGTCTT 954 0.16310731401235443 No Hit TGCAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGG 923 0.15780718116708925 No Hit AATGATCTGTCTCTTATACACATCTGACGCGACGATACTCGTATGCCGTCT 840 0.1436165029039599 No Hit AAAAACTGTCTCTTATACACATCTGACGCGACGATACTCGTATGCCGTCTT 810 0.13848734208596133 No Hit AACTGTCTCTTATACACATCTGACGCGACGATACTCGTATGCCGTCTTCTG 739 0.12634832815003136 No Hit CCTGTCTCTTATACACATCTGACGCGACGATACTCGTATGCCGTCTTCTGC 728 0.12446763585009857 TruSeq Adapter, Index 20 (95% over 21bp) AATGATACGGCGACTGTCTCTTATACACATCTGACGCGACGATACTCGTAT 640 0.1094220974506361 No Hit AATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGACGATACTC 587 0.1003605800055053 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 1.709720272666189E-4 0.0 0.0 0.8269916958886356 0.0 2 1.709720272666189E-4 0.0 0.0 1.1232862191416861 0.0 3 1.709720272666189E-4 0.0 0.0 1.5784137557254259 0.0 4 1.709720272666189E-4 0.0 0.0 3.6668370687871756 0.0 5 1.709720272666189E-4 0.0 0.0 3.9769803262488224 0.0 6 1.709720272666189E-4 0.0 0.0 5.3189397682645145 0.0 7 1.709720272666189E-4 0.0 0.0 6.413502686825408 0.0 8 1.709720272666189E-4 0.0 0.0 7.17347334802553 0.0 9 1.709720272666189E-4 0.0 0.0 8.86130920120159 0.0 10 1.709720272666189E-4 0.0 0.0 10.39099592915603 0.0 11 1.709720272666189E-4 0.0 0.0 12.406585158602201 0.0 12 1.709720272666189E-4 0.0 0.0 13.114409351486003 0.0 13 1.709720272666189E-4 0.0 0.0 13.431391490038315 0.0 14 1.709720272666189E-4 0.0 0.0 13.873354180522524 0.0 15 1.709720272666189E-4 0.0 0.0 14.180932857575172 0.0 16 1.709720272666189E-4 0.0 0.0 14.800193540334865 0.0 17 1.709720272666189E-4 0.0 0.0 15.511095229709467 0.0 18 1.709720272666189E-4 0.0 0.0 16.64720435089615 0.0 19 1.709720272666189E-4 0.0 0.0 17.050014447136306 0.0 20 1.709720272666189E-4 0.0 0.0 17.53831055700977 0.0 21 1.709720272666189E-4 0.0 0.0 17.99378003764804 0.0 22 1.709720272666189E-4 0.0 0.0 18.494728077539232 0.0 23 1.709720272666189E-4 0.0 0.0 19.003027914602892 0.0 24 1.709720272666189E-4 0.0 0.0 19.384124563380187 0.0 25 1.709720272666189E-4 0.0 0.0 19.705722946668697 0.0 26 1.709720272666189E-4 0.0 0.0 20.02048244886654 0.0 27 1.709720272666189E-4 0.0 0.0 20.36533302786331 0.0 28 1.709720272666189E-4 0.0 0.0 20.726596921477675 0.0 29 1.709720272666189E-4 0.0 0.0 21.08580915076484 0.0 30 1.709720272666189E-4 0.0 0.0 21.606931889873497 0.0 31 1.709720272666189E-4 0.0 0.0 21.920152643825944 0.0 32 1.709720272666189E-4 0.0 0.0 22.274577656349646 0.0 33 1.709720272666189E-4 0.0 0.0 22.62626711643708 0.0 34 1.709720272666189E-4 0.0 0.0 22.980350184906246 0.0 35 1.709720272666189E-4 0.0 0.0 23.348111015556746 0.0 36 1.709720272666189E-4 0.0 0.0 23.691764790362647 0.0 37 1.709720272666189E-4 0.0 0.0 24.021569830959958 0.0 38 1.709720272666189E-4 0.0 0.0 24.407966612582516 0.0 39 1.709720272666189E-4 0.0 0.0 24.808554072468205 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGCGATT 35 1.2105556E-7 45.000004 11 TCTAGCG 70 0.0 45.000004 1 TAGCACA 35 1.2105556E-7 45.000004 11 TGATTCG 35 1.2105556E-7 45.000004 15 TAACACG 35 1.2105556E-7 45.000004 1 TATCGGT 35 1.2105556E-7 45.000004 29 CTTCGTC 25 3.888448E-5 45.0 41 AGGTATC 20 7.030441E-4 45.0 33 AGCGTAA 20 7.030441E-4 45.0 1 AGCCGAT 25 3.888448E-5 45.0 12 TCGTTAT 25 3.888448E-5 45.0 43 TCGCACG 20 7.030441E-4 45.0 1 ACGCCTA 25 3.888448E-5 45.0 43 CTCGTCA 50 2.1827873E-11 45.0 36 CGTATAG 20 7.030441E-4 45.0 1 AATCACC 20 7.030441E-4 45.0 23 ACGTTTG 20 7.030441E-4 45.0 45 CCGATTC 20 7.030441E-4 45.0 24 CGAAACG 25 3.888448E-5 45.0 1 ACGTTGT 20 7.030441E-4 45.0 11 >>END_MODULE