Basic Statistics
Measure | Value |
---|---|
Filename | SRR3548422_1.fastq |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 651269 |
Sequences flagged as poor quality | 0 |
Sequence length | 51 |
%GC | 49 |
Per base sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
AATGATACCTGTCTCTTATACACATCTGACGCTCCTTGGTTCGTATGCCGT | 10917 | 1.676265874776782 | No Hit |
AATGATACGGCTGTCTCTTATACACATCTGACGCTCCTTGGTTCGTATGCC | 10898 | 1.6733484934796528 | No Hit |
AATCTGTCTCTTATACACATCTGACGCTCCTTGGTTCGTATGCCGTCTTCT | 9298 | 1.4276742789845671 | No Hit |
CTGTCTCTTATACACATCTGACGCTCCTTGGTTCGTATGCCGTCTTCTGCT | 4785 | 0.7347194477243658 | Illumina Single End Adapter 2 (95% over 21bp) |
AATGACTGTCTCTTATACACATCTGACGCTCCTTGGTTCGTATGCCGTCTT | 2636 | 0.4047482683806538 | No Hit |
AATGATCTGTCTCTTATACACATCTGACGCTCCTTGGTTCGTATGCCGTCT | 2074 | 0.3184552005392549 | No Hit |
AATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCTCCTTGGTTC | 1700 | 0.2610288529010286 | No Hit |
AACTGTCTCTTATACACATCTGACGCTCCTTGGTTCGTATGCCGTCTTCTG | 1649 | 0.2531979873139978 | No Hit |
AATGATACGGCGACTGTCTCTTATACACATCTGACGCTCCTTGGTTCGTAT | 1478 | 0.22694155563983548 | No Hit |
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA | 1395 | 0.2141972057629029 | No Hit |
AAACTGTCTCTTATACACATCTGACGCTCCTTGGTTCGTATGCCGTCTTCT | 788 | 0.12099455063882972 | No Hit |
CCTGTCTCTTATACACATCTGACGCTCCTTGGTTCGTATGCCGTCTTCTGC | 788 | 0.12099455063882972 | No Hit |
TGCAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGG | 761 | 0.11684879826922515 | No Hit |
AATGCTGTCTCTTATACACATCTGACGCTCCTTGGTTCGTATGCCGTCTTC | 722 | 0.11086048929090744 | No Hit |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
TCACGCA | 20 | 7.0310314E-4 | 45.000004 | 24 |
CAGCGCG | 20 | 7.0310314E-4 | 45.000004 | 1 |
GATACGT | 20 | 7.0310314E-4 | 45.000004 | 9 |
CGTATAG | 30 | 2.1640462E-6 | 45.000004 | 1 |
TCCGATC | 20 | 7.0310314E-4 | 45.000004 | 35 |
ACTTGCG | 20 | 7.0310314E-4 | 45.000004 | 1 |
GACGTCG | 20 | 7.0310314E-4 | 45.000004 | 1 |
GATCACG | 20 | 7.0310314E-4 | 45.000004 | 1 |
ATCGTTA | 20 | 7.0310314E-4 | 45.000004 | 22 |
AATTGCG | 40 | 6.8066583E-9 | 45.000004 | 1 |
CTACGAA | 30 | 2.1640462E-6 | 45.000004 | 10 |
CTTGCCG | 20 | 7.0310314E-4 | 45.000004 | 1 |
GCTTACG | 30 | 2.1640462E-6 | 45.000004 | 1 |
CGTACCG | 20 | 7.0310314E-4 | 45.000004 | 1 |
ACGTCCA | 20 | 7.0310314E-4 | 45.000004 | 45 |
AGCTACG | 30 | 2.1640462E-6 | 45.000004 | 8 |
TCGACCA | 20 | 7.0310314E-4 | 45.000004 | 14 |
TATGTCG | 20 | 7.0310314E-4 | 45.000004 | 1 |
CGAGTCA | 25 | 3.8889375E-5 | 45.0 | 14 |
CCGATCA | 25 | 3.8889375E-5 | 45.0 | 17 |