FastQCFastQC Report
Sat 18 Jun 2016
SRR3548422_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3548422_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences651269
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AATGATACCTGTCTCTTATACACATCTGACGCTCCTTGGTTCGTATGCCGT109171.676265874776782No Hit
AATGATACGGCTGTCTCTTATACACATCTGACGCTCCTTGGTTCGTATGCC108981.6733484934796528No Hit
AATCTGTCTCTTATACACATCTGACGCTCCTTGGTTCGTATGCCGTCTTCT92981.4276742789845671No Hit
CTGTCTCTTATACACATCTGACGCTCCTTGGTTCGTATGCCGTCTTCTGCT47850.7347194477243658Illumina Single End Adapter 2 (95% over 21bp)
AATGACTGTCTCTTATACACATCTGACGCTCCTTGGTTCGTATGCCGTCTT26360.4047482683806538No Hit
AATGATCTGTCTCTTATACACATCTGACGCTCCTTGGTTCGTATGCCGTCT20740.3184552005392549No Hit
AATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCTCCTTGGTTC17000.2610288529010286No Hit
AACTGTCTCTTATACACATCTGACGCTCCTTGGTTCGTATGCCGTCTTCTG16490.2531979873139978No Hit
AATGATACGGCGACTGTCTCTTATACACATCTGACGCTCCTTGGTTCGTAT14780.22694155563983548No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA13950.2141972057629029No Hit
AAACTGTCTCTTATACACATCTGACGCTCCTTGGTTCGTATGCCGTCTTCT7880.12099455063882972No Hit
CCTGTCTCTTATACACATCTGACGCTCCTTGGTTCGTATGCCGTCTTCTGC7880.12099455063882972No Hit
TGCAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGG7610.11684879826922515No Hit
AATGCTGTCTCTTATACACATCTGACGCTCCTTGGTTCGTATGCCGTCTTC7220.11086048929090744No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCACGCA207.0310314E-445.00000424
CAGCGCG207.0310314E-445.0000041
GATACGT207.0310314E-445.0000049
CGTATAG302.1640462E-645.0000041
TCCGATC207.0310314E-445.00000435
ACTTGCG207.0310314E-445.0000041
GACGTCG207.0310314E-445.0000041
GATCACG207.0310314E-445.0000041
ATCGTTA207.0310314E-445.00000422
AATTGCG406.8066583E-945.0000041
CTACGAA302.1640462E-645.00000410
CTTGCCG207.0310314E-445.0000041
GCTTACG302.1640462E-645.0000041
CGTACCG207.0310314E-445.0000041
ACGTCCA207.0310314E-445.00000445
AGCTACG302.1640462E-645.0000048
TCGACCA207.0310314E-445.00000414
TATGTCG207.0310314E-445.0000041
CGAGTCA253.8889375E-545.014
CCGATCA253.8889375E-545.017