FastQCFastQC Report
Sat 18 Jun 2016
SRR3548420_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3548420_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences364001
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTCTCTTATACACATCTGACGCCTTCTCTGTCGTATGCCGTCTTCTGCT33800.9285688775580286TruSeq Adapter, Index 13 (95% over 22bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA18000.4945041359776485No Hit
AAACTGTCTCTTATACACATCTGACGCCTTCTCTGTCGTATGCCGTCTTCT13790.3788451130628762No Hit
AATGATACCTGTCTCTTATACACATCTGACGCCTTCTCTGTCGTATGCCGT12690.34862541586424217No Hit
AATGATACGGCTGTCTCTTATACACATCTGACGCCTTCTCTGTCGTATGCC12670.34807596682426695No Hit
AATCTGTCTCTTATACACATCTGACGCCTTCTCTGTCGTATGCCGTCTTCT12570.34532872162439116No Hit
AAAAACTGTCTCTTATACACATCTGACGCCTTCTCTGTCGTATGCCGTCTT9690.26620805986796736No Hit
CCTGTCTCTTATACACATCTGACGCCTTCTCTGTCGTATGCCGTCTTCTGC6130.16840613075238806TruSeq Adapter, Index 16 (95% over 21bp)
AAAAAACTGTCTCTTATACACATCTGACGCCTTCTCTGTCGTATGCCGTCT5710.15686770091290958No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4380.1203293397545611No Hit
GAAAAACTGTCTCTTATACACATCTGACGCCTTCTCTGTCGTATGCCGTCT3760.10329641951533101No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCCCGT207.026927E-445.00000420
AACGTCC207.026927E-445.00000422
TCCGCAT207.026927E-445.00000441
ATTAGCT406.7921064E-945.00000410
CAACGCT207.026927E-445.00000426
ACTTGCA207.026927E-445.00000431
ATAATCG207.026927E-445.0000041
GTCCTAA207.026927E-445.00000425
TAGCGTC207.026927E-445.00000414
CGGCACT207.026927E-445.00000435
CGGCAAC207.026927E-445.00000430
GGATCTC207.026927E-445.0000048
ACCGAGT207.026927E-445.00000425
ATGCTTC207.026927E-445.00000411
TTCGTGT207.026927E-445.00000412
CGACATT207.026927E-445.00000410
ATCACGT207.026927E-445.00000444
CGTTAAC207.026927E-445.0000045
GTTACAC207.026927E-445.0000049
GTAGCGT207.026927E-445.00000413