Basic Statistics
Measure | Value |
---|---|
Filename | SRR3548420_1.fastq |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 364001 |
Sequences flagged as poor quality | 0 |
Sequence length | 51 |
%GC | 49 |
Per base sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
CTGTCTCTTATACACATCTGACGCCTTCTCTGTCGTATGCCGTCTTCTGCT | 3380 | 0.9285688775580286 | TruSeq Adapter, Index 13 (95% over 22bp) |
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA | 1800 | 0.4945041359776485 | No Hit |
AAACTGTCTCTTATACACATCTGACGCCTTCTCTGTCGTATGCCGTCTTCT | 1379 | 0.3788451130628762 | No Hit |
AATGATACCTGTCTCTTATACACATCTGACGCCTTCTCTGTCGTATGCCGT | 1269 | 0.34862541586424217 | No Hit |
AATGATACGGCTGTCTCTTATACACATCTGACGCCTTCTCTGTCGTATGCC | 1267 | 0.34807596682426695 | No Hit |
AATCTGTCTCTTATACACATCTGACGCCTTCTCTGTCGTATGCCGTCTTCT | 1257 | 0.34532872162439116 | No Hit |
AAAAACTGTCTCTTATACACATCTGACGCCTTCTCTGTCGTATGCCGTCTT | 969 | 0.26620805986796736 | No Hit |
CCTGTCTCTTATACACATCTGACGCCTTCTCTGTCGTATGCCGTCTTCTGC | 613 | 0.16840613075238806 | TruSeq Adapter, Index 16 (95% over 21bp) |
AAAAAACTGTCTCTTATACACATCTGACGCCTTCTCTGTCGTATGCCGTCT | 571 | 0.15686770091290958 | No Hit |
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA | 438 | 0.1203293397545611 | No Hit |
GAAAAACTGTCTCTTATACACATCTGACGCCTTCTCTGTCGTATGCCGTCT | 376 | 0.10329641951533101 | No Hit |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
GCCCCGT | 20 | 7.026927E-4 | 45.000004 | 20 |
AACGTCC | 20 | 7.026927E-4 | 45.000004 | 22 |
TCCGCAT | 20 | 7.026927E-4 | 45.000004 | 41 |
ATTAGCT | 40 | 6.7921064E-9 | 45.000004 | 10 |
CAACGCT | 20 | 7.026927E-4 | 45.000004 | 26 |
ACTTGCA | 20 | 7.026927E-4 | 45.000004 | 31 |
ATAATCG | 20 | 7.026927E-4 | 45.000004 | 1 |
GTCCTAA | 20 | 7.026927E-4 | 45.000004 | 25 |
TAGCGTC | 20 | 7.026927E-4 | 45.000004 | 14 |
CGGCACT | 20 | 7.026927E-4 | 45.000004 | 35 |
CGGCAAC | 20 | 7.026927E-4 | 45.000004 | 30 |
GGATCTC | 20 | 7.026927E-4 | 45.000004 | 8 |
ACCGAGT | 20 | 7.026927E-4 | 45.000004 | 25 |
ATGCTTC | 20 | 7.026927E-4 | 45.000004 | 11 |
TTCGTGT | 20 | 7.026927E-4 | 45.000004 | 12 |
CGACATT | 20 | 7.026927E-4 | 45.000004 | 10 |
ATCACGT | 20 | 7.026927E-4 | 45.000004 | 44 |
CGTTAAC | 20 | 7.026927E-4 | 45.000004 | 5 |
GTTACAC | 20 | 7.026927E-4 | 45.000004 | 9 |
GTAGCGT | 20 | 7.026927E-4 | 45.000004 | 13 |