FastQCFastQC Report
Sat 18 Jun 2016
SRR3548418_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3548418_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1003695
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AATGATACCTGTCTCTTATACACATCTGACGCCACTAACCTCGTATGCCGT428894.273110855389336No Hit
AATGATACGGCTGTCTCTTATACACATCTGACGCCACTAACCTCGTATGCC418444.168995561400625No Hit
AATCTGTCTCTTATACACATCTGACGCCACTAACCTCGTATGCCGTCTTCT394463.9300783604581073TruSeq Adapter, Index 12 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCCACTAACCTCGTATGCCGTCTTCTGCT119461.1902022028604307RNA PCR Primer, Index 29 (96% over 26bp)
AATGACTGTCTCTTATACACATCTGACGCCACTAACCTCGTATGCCGTCTT104181.0379647203582762No Hit
AATGATCTGTCTCTTATACACATCTGACGCCACTAACCTCGTATGCCGTCT86710.8639078604556165No Hit
AACTGTCTCTTATACACATCTGACGCCACTAACCTCGTATGCCGTCTTCTG77320.7703535436561904RNA PCR Primer, Index 29 (95% over 24bp)
AATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCCACTAACCTC64430.6419280757600666No Hit
AATGATACGGCGACTGTCTCTTATACACATCTGACGCCACTAACCTCGTAT60380.6015771723481735No Hit
AATGCTGTCTCTTATACACATCTGACGCCACTAACCTCGTATGCCGTCTTC30380.30268159151933605No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA17520.17455501920404107No Hit
AATGATACGGCGCTGTCTCTTATACACATCTGACGCCACTAACCTCGTATG17430.17365833246155454No Hit
AATGATACGGCGACCTGTCTCTTATACACATCTGACGCCACTAACCTCGTA15940.15881318528038896No Hit
AATGATACGGCCTGTCTCTTATACACATCTGACGCCACTAACCTCGTATGC15550.15492754272961407No Hit
AATGATACGGCGACCACCCTGTCTCTTATACACATCTGACGCCACTAACCT12380.12334424302203359No Hit
CCTGTCTCTTATACACATCTGACGCCACTAACCTCGTATGCCGTCTTCTGC10990.1094954144436308RNA PCR Primer, Index 29 (96% over 25bp)
AAACTGTCTCTTATACACATCTGACGCCACTAACCTCGTATGCCGTCTTCT10890.108499095840868No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AATCCCG207.0328574E-445.0000041
GGTACGT207.0328574E-445.0000049
ATAGGCG207.0328574E-445.0000041
CGAAAAG302.165225E-645.0000041
TAAACCG406.8139343E-945.0000041
TCGATAG207.0328574E-445.0000041
CGTTCCG207.0328574E-445.00000423
GCTTGCG207.0328574E-445.0000041
TTATCCG302.165225E-645.0000041
TAGCCGT406.8139343E-945.00000444
GCCGCGC207.0328574E-445.00000432
CCGTAGT551.8189894E-1245.00000427
CATAGCG406.8139343E-945.0000041
CGTAGCG406.8139343E-945.0000041
ACGGAGT207.0328574E-445.00000433
GTCCGGA207.0328574E-445.00000426
TCCGTTG207.0328574E-445.00000431
AGTTCGG302.165225E-645.0000042
ACGCTAT207.0328574E-445.00000434
CGAGCAT207.0328574E-445.00000426
Produced by FastQC (version 0.11.5)