##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3548418_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1003695
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.441076223354706	31.0	31.0	34.0	30.0	34.0
2	31.654597263112798	31.0	31.0	34.0	30.0	34.0
3	31.738775225541623	31.0	31.0	34.0	30.0	34.0
4	35.41457414852121	37.0	35.0	37.0	33.0	37.0
5	35.09932200519082	37.0	35.0	37.0	32.0	37.0
6	34.468498896577145	37.0	35.0	37.0	32.0	37.0
7	34.663489406642455	37.0	35.0	37.0	31.0	37.0
8	35.263150658317514	37.0	35.0	37.0	32.0	37.0
9	37.01800148451472	39.0	37.0	39.0	33.0	39.0
10	36.47575508496107	38.0	35.0	39.0	32.0	39.0
11	36.518076706569225	38.0	35.0	39.0	32.0	39.0
12	36.24245313566372	38.0	35.0	39.0	32.0	39.0
13	36.437353977054784	38.0	35.0	39.0	32.0	39.0
14	37.3667149881189	39.0	36.0	41.0	32.0	41.0
15	37.603197186396265	39.0	36.0	41.0	32.0	41.0
16	37.15833993394408	39.0	36.0	41.0	31.0	41.0
17	37.184008090107056	39.0	36.0	40.0	31.0	41.0
18	37.01534928439416	39.0	36.0	40.0	31.0	41.0
19	36.8798389949138	39.0	35.0	40.0	30.0	41.0
20	37.205585362087085	39.0	36.0	40.0	31.0	41.0
21	37.313595265494	39.0	36.0	40.0	32.0	41.0
22	37.25042567712303	39.0	36.0	40.0	32.0	41.0
23	37.093546346250605	39.0	36.0	40.0	31.0	41.0
24	37.046531067704834	39.0	36.0	40.0	31.0	41.0
25	37.07303613149413	39.0	36.0	40.0	31.0	41.0
26	36.956724901489	39.0	36.0	40.0	31.0	41.0
27	36.86631197724408	39.0	36.0	40.0	31.0	41.0
28	36.82024419768954	39.0	35.0	40.0	30.0	41.0
29	36.63773955235405	39.0	35.0	40.0	30.0	41.0
30	36.9523988861158	39.0	36.0	40.0	31.0	41.0
31	36.63150757949377	39.0	36.0	40.0	30.0	41.0
32	36.475558810196326	39.0	35.0	40.0	30.0	41.0
33	36.3548866936669	39.0	35.0	40.0	30.0	41.0
34	36.294757869671564	39.0	35.0	40.0	30.0	41.0
35	36.439376503818394	39.0	35.0	40.0	30.0	41.0
36	36.37767947434231	39.0	35.0	40.0	30.0	41.0
37	36.463123757715245	39.0	35.0	40.0	30.0	41.0
38	36.14518852838761	38.0	35.0	40.0	30.0	41.0
39	35.92530997962528	38.0	35.0	40.0	28.0	41.0
40	35.814793338613825	38.0	35.0	40.0	27.0	41.0
41	36.02200768161643	38.0	35.0	40.0	29.0	41.0
42	36.20896288215045	39.0	35.0	40.0	30.0	41.0
43	36.12131673466541	38.0	35.0	40.0	30.0	41.0
44	36.158073916877136	38.0	35.0	40.0	30.0	41.0
45	35.792035429089516	38.0	35.0	40.0	28.0	41.0
46	35.40511011811357	38.0	34.0	40.0	27.0	41.0
47	35.54194949661002	38.0	34.0	40.0	28.0	41.0
48	35.547863643836024	38.0	34.0	40.0	28.0	41.0
49	35.484854462760104	38.0	34.0	40.0	27.0	41.0
50	35.520110192837464	38.0	34.0	40.0	28.0	41.0
51	33.79945302108708	37.0	32.0	40.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	4.0
10	0.0
11	5.0
12	8.0
13	9.0
14	12.0
15	12.0
16	50.0
17	118.0
18	254.0
19	786.0
20	1834.0
21	3527.0
22	5114.0
23	6082.0
24	6647.0
25	6687.0
26	7431.0
27	8705.0
28	11287.0
29	15182.0
30	21542.0
31	29761.0
32	40587.0
33	53453.0
34	70920.0
35	87019.0
36	102561.0
37	131695.0
38	189489.0
39	202868.0
40	46.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	14.901638445942245	46.666068875505005	15.930636298875655	22.501656379677094
2	17.678876551143524	45.094675175227536	16.54715825026527	20.679290023363674
3	16.363935259217193	23.621418857322194	38.01553260701707	21.99911327644354
4	32.41074230717499	24.101843687574412	17.22993538873861	26.257478616511985
5	15.198342125845002	42.401725623819985	18.58851543546595	23.81141681486906
6	19.547372458764865	29.014591085937464	32.333627247321154	19.104409207976527
7	66.41290431854299	17.560513901135305	9.836753196937316	6.189828583384395
8	68.79041940031583	2.9770996169154973	6.054329253408655	22.178151729360014
9	72.02656185394966	6.484340362361076	12.51296459581845	8.976133187870818
10	31.8235121227066	35.592286501377416	15.916787470297253	16.667413905618737
11	20.175252442225975	21.53831592266575	33.443326907078344	24.843104728029928
12	22.660270301236928	20.08299333961014	41.622405212738926	15.634331146413999
13	23.1232595559408	28.390995272468228	25.82039364547995	22.665351526111017
14	14.733659129516436	24.31555402786703	40.11367995257523	20.837106890041298
15	14.028365190620656	29.33650162649012	25.7618101116375	30.873323071251725
16	16.690030337901455	28.745983590632612	33.30125187432437	21.26273419714156
17	14.768729544333686	27.99874463856052	30.356233716417837	26.87629210068796
18	16.72221142877069	28.36070718694424	26.808642067560363	28.108439316724702
19	17.858213899640827	30.3160820767265	34.645385301311656	17.180318722321026
20	19.526748663687673	35.261010565958784	26.47756539586229	18.734675374491257
21	17.328072771110744	25.308883674821537	30.826296833201322	26.5367467208664
22	18.957452214069015	31.349961890813443	28.785437807302017	20.90714808781552
23	21.72442823766184	26.81890414916882	23.042258853536186	28.414408759633154
24	14.79114671289585	39.09554197241194	24.46211249433344	21.651198820358776
25	18.150135250250322	24.088393386437115	28.93388927911367	28.827582084198884
26	24.36048799685163	30.013799012648263	21.547880581252272	24.07783240924783
27	15.555721608656018	26.090993777990324	30.767015876336934	27.586268737016724
28	21.145467497596382	23.89122193495036	25.74975465654407	29.21355591090919
29	16.96063047041183	37.48857969801583	25.200085683399838	20.3507041481725
30	21.029994171536174	22.61254664016459	22.94920269603814	33.408256492261096
31	22.50444607176483	30.400569894240782	25.541225172985815	21.553758861008575
32	18.054189768804267	29.375358051997868	20.899775330155077	31.67067684904279
33	20.56820049915562	28.241447850193534	23.076034054169842	28.114317596481005
34	15.51736334244965	28.994365818301375	23.558052994186482	31.930217845062494
35	14.695101599589517	25.726241537518867	22.582258554640603	36.99639830825101
36	15.272169334309726	30.068098376498835	31.826301814794334	22.833430474397105
37	16.654661027503376	29.473595066230278	20.4128744289849	33.458869477281446
38	19.911825803655493	29.654227628911173	29.214054070210572	21.219892497222762
39	15.808188742596109	28.606399354385548	26.28637185599211	29.29904004702624
40	19.040545185539433	30.826396465061595	21.139489585979803	28.993568763419162
41	16.260816283831243	21.92259600775136	33.499618908134444	28.316968800282954
42	22.12574537085469	23.227574113650064	21.71047977722316	32.936200738272085
43	20.66215334339615	24.06049646555976	28.26107532666796	27.01627486437613
44	19.277071221835318	20.304773860585136	26.611271352353054	33.806883565226485
45	27.861153039518978	25.308186251799604	24.79129616068626	22.03936454799516
46	14.921863713578329	23.65768485446276	38.06953307528681	23.350918356672096
47	21.469669570935395	27.953611405855366	22.732503399937233	27.84421562327201
48	17.00237621986759	20.9055539780511	35.83947314672286	26.252596655358452
49	21.632468030626832	19.950582597302965	29.365494497830518	29.051454874239685
50	19.943608367083627	21.088976232819732	25.483438694025573	33.48397670607107
51	15.493551327843619	19.415858403200176	37.36035349384026	27.730236775115948
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	2185.0
1	1719.5
2	1254.0
3	892.5
4	531.0
5	529.0
6	527.0
7	590.5
8	654.0
9	689.5
10	725.0
11	978.5
12	1232.0
13	1255.0
14	1278.0
15	1358.5
16	1439.0
17	1753.0
18	2067.0
19	2056.5
20	2046.0
21	2536.0
22	3026.0
23	3793.0
24	4560.0
25	4940.5
26	6879.5
27	8438.0
28	9790.5
29	11143.0
30	12660.5
31	14178.0
32	17123.0
33	20068.0
34	22961.0
35	25854.0
36	28388.0
37	30922.0
38	31730.5
39	32539.0
40	34612.5
41	36686.0
42	42133.5
43	47581.0
44	108817.0
45	170053.0
46	156848.5
47	143644.0
48	126351.0
49	109058.0
50	96389.0
51	83720.0
52	73390.0
53	63060.0
54	55575.5
55	48091.0
56	41606.0
57	35121.0
58	31928.5
59	28736.0
60	25714.5
61	22693.0
62	19778.0
63	16863.0
64	13688.0
65	10513.0
66	8236.5
67	5960.0
68	4832.5
69	3705.0
70	3164.5
71	2624.0
72	2234.0
73	1844.0
74	1619.0
75	1277.0
76	1160.0
77	851.0
78	542.0
79	527.0
80	512.0
81	299.0
82	86.0
83	61.5
84	37.0
85	23.0
86	9.0
87	8.0
88	7.0
89	6.5
90	6.0
91	3.0
92	0.0
93	1.0
94	2.0
95	1.5
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1003695.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	24.167355571883366
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.3389602500424	17.965760852068787
2	10.954562375273428	5.294856081152163
3	4.067605220103733	2.949097850408875
4	2.014490121824868	1.9473959628075292
5	1.2401890581327553	1.4986044972126715
6	0.8042235273995995	1.1661573567564245
7	0.5966801294075403	1.009412659504859
8	0.4699669221210956	0.9086286171139308
9	0.37579953780977443	0.8173872948598421
>10	3.969549819028538	21.803680297015994
>50	0.8824965474416	14.831171759715984
>100	0.27160208166994315	9.63197039369119
>500	0.006726986542908809	1.0191324758555058
>1k	0.0033634932714544045	1.3318831997521403
>5k	0.0016817466357272022	2.9348576702502913
>10k+	0.002102183294659003	14.890003031833835
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AATGATACCTGTCTCTTATACACATCTGACGCCACTAACCTCGTATGCCGT	42889	4.273110855389336	No Hit
AATGATACGGCTGTCTCTTATACACATCTGACGCCACTAACCTCGTATGCC	41844	4.168995561400625	No Hit
AATCTGTCTCTTATACACATCTGACGCCACTAACCTCGTATGCCGTCTTCT	39446	3.9300783604581073	TruSeq Adapter, Index 12 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCCACTAACCTCGTATGCCGTCTTCTGCT	11946	1.1902022028604307	RNA PCR Primer, Index 29 (96% over 26bp)
AATGACTGTCTCTTATACACATCTGACGCCACTAACCTCGTATGCCGTCTT	10418	1.0379647203582762	No Hit
AATGATCTGTCTCTTATACACATCTGACGCCACTAACCTCGTATGCCGTCT	8671	0.8639078604556165	No Hit
AACTGTCTCTTATACACATCTGACGCCACTAACCTCGTATGCCGTCTTCTG	7732	0.7703535436561904	RNA PCR Primer, Index 29 (95% over 24bp)
AATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCCACTAACCTC	6443	0.6419280757600666	No Hit
AATGATACGGCGACTGTCTCTTATACACATCTGACGCCACTAACCTCGTAT	6038	0.6015771723481735	No Hit
AATGCTGTCTCTTATACACATCTGACGCCACTAACCTCGTATGCCGTCTTC	3038	0.30268159151933605	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1752	0.17455501920404107	No Hit
AATGATACGGCGCTGTCTCTTATACACATCTGACGCCACTAACCTCGTATG	1743	0.17365833246155454	No Hit
AATGATACGGCGACCTGTCTCTTATACACATCTGACGCCACTAACCTCGTA	1594	0.15881318528038896	No Hit
AATGATACGGCCTGTCTCTTATACACATCTGACGCCACTAACCTCGTATGC	1555	0.15492754272961407	No Hit
AATGATACGGCGACCACCCTGTCTCTTATACACATCTGACGCCACTAACCT	1238	0.12334424302203359	No Hit
CCTGTCTCTTATACACATCTGACGCCACTAACCTCGTATGCCGTCTTCTGC	1099	0.1094954144436308	RNA PCR Primer, Index 29 (96% over 25bp)
AAACTGTCTCTTATACACATCTGACGCCACTAACCTCGTATGCCGTCTTCT	1089	0.108499095840868	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	9.963186027627915E-5	0.0	0.0	1.33267576305551	0.0
2	9.963186027627915E-5	0.0	0.0	1.6405382113092124	0.0
3	9.963186027627915E-5	0.0	0.0	2.8290466725449464	0.0
4	9.963186027627915E-5	0.0	0.0	8.440811202606369	0.0
5	9.963186027627915E-5	0.0	0.0	8.990480175750601	0.0
6	9.963186027627915E-5	0.0	0.0	11.172417915801114	0.0
7	9.963186027627915E-5	0.0	0.0	12.942577177329767	0.0
8	9.963186027627915E-5	0.0	0.0	13.683240426623625	0.0
9	9.963186027627915E-5	0.0	0.0	19.28484250693687	0.0
10	9.963186027627915E-5	0.0	0.0	20.69184363775848	0.0
11	9.963186027627915E-5	0.0	0.0	26.56294990011906	0.0
12	9.963186027627915E-5	0.0	0.0	27.442101435196946	0.0
13	9.963186027627915E-5	0.0	0.0	27.986489919746536	0.0
14	9.963186027627915E-5	0.0	0.0	28.981015149024355	0.0
15	9.963186027627915E-5	0.0	0.0	29.432746003517003	0.0
16	9.963186027627915E-5	0.0	0.0	30.037411763533743	0.0
17	9.963186027627915E-5	0.0	0.0	30.762731706345054	0.0
18	9.963186027627915E-5	0.0	0.0	32.55441144969338	0.0
19	9.963186027627915E-5	0.0	0.0	33.111752076078886	0.0
20	9.963186027627915E-5	0.0	0.0	33.5640807217332	0.0
21	9.963186027627915E-5	0.0	0.0	34.14553225830556	0.0
22	9.963186027627915E-5	0.0	0.0	34.71293570257897	0.0
23	9.963186027627915E-5	0.0	0.0	35.24546799575568	0.0
24	9.963186027627915E-5	0.0	0.0	35.620083790394496	0.0
25	9.963186027627915E-5	0.0	0.0	35.92694992004543	0.0
26	9.963186027627915E-5	0.0	0.0	36.231026357608634	0.0
27	9.963186027627915E-5	0.0	0.0	36.57605148974539	0.0
28	9.963186027627915E-5	0.0	0.0	36.91529797398612	0.0
29	9.963186027627915E-5	0.0	0.0	37.28353732956725	0.0
30	9.963186027627915E-5	0.0	0.0	37.86299622893409	0.0
31	9.963186027627915E-5	0.0	0.0	38.204833141542004	0.0
32	9.963186027627915E-5	0.0	0.0	38.53670686812229	0.0
33	9.963186027627915E-5	0.0	0.0	38.87216734167252	0.0
34	9.963186027627915E-5	0.0	0.0	39.185310278520866	0.0
35	9.963186027627915E-5	0.0	0.0	39.56271576524741	0.0
36	9.963186027627915E-5	0.0	0.0	39.90644568320057	0.0
37	9.963186027627915E-5	0.0	0.0	40.25097265603595	0.0
38	9.963186027627915E-5	0.0	0.0	40.58434086052038	0.0
39	9.963186027627915E-5	0.0	0.0	40.93504500869288	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AATCCCG	20	7.0328574E-4	45.000004	1
GGTACGT	20	7.0328574E-4	45.000004	9
ATAGGCG	20	7.0328574E-4	45.000004	1
CGAAAAG	30	2.165225E-6	45.000004	1
TAAACCG	40	6.8139343E-9	45.000004	1
TCGATAG	20	7.0328574E-4	45.000004	1
CGTTCCG	20	7.0328574E-4	45.000004	23
GCTTGCG	20	7.0328574E-4	45.000004	1
TTATCCG	30	2.165225E-6	45.000004	1
TAGCCGT	40	6.8139343E-9	45.000004	44
GCCGCGC	20	7.0328574E-4	45.000004	32
CCGTAGT	55	1.8189894E-12	45.000004	27
CATAGCG	40	6.8139343E-9	45.000004	1
CGTAGCG	40	6.8139343E-9	45.000004	1
ACGGAGT	20	7.0328574E-4	45.000004	33
GTCCGGA	20	7.0328574E-4	45.000004	26
TCCGTTG	20	7.0328574E-4	45.000004	31
AGTTCGG	30	2.165225E-6	45.000004	2
ACGCTAT	20	7.0328574E-4	45.000004	34
CGAGCAT	20	7.0328574E-4	45.000004	26
>>END_MODULE