FastQC Report![[OK]](data:image/png;base64,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)
Basic Statistics
| Measure | Value |
|---|---|
| Filename | SRR3548417_1.fastq |
| File type | Conventional base calls |
| Encoding | Sanger / Illumina 1.9 |
| Total Sequences | 1045999 |
| Sequences flagged as poor quality | 0 |
| Sequence length | 51 |
| %GC | 46 |
Per base sequence quality
Per sequence quality scores
![[FAIL]](data:image/png;base64,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)
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
| Sequence | Count | Percentage | Possible Source |
|---|---|---|---|
| AATGATACGGCTGTCTCTTATACACATCTGACGCCACTAACCTCGTATGCC | 49001 | 4.684612509189779 | No Hit |
| AATGATACCTGTCTCTTATACACATCTGACGCCACTAACCTCGTATGCCGT | 48707 | 4.656505407748956 | No Hit |
| AATCTGTCTCTTATACACATCTGACGCCACTAACCTCGTATGCCGTCTTCT | 44768 | 4.2799276098734325 | TruSeq Adapter, Index 12 (95% over 21bp) |
| CTGTCTCTTATACACATCTGACGCCACTAACCTCGTATGCCGTCTTCTGCT | 13314 | 1.2728501652487239 | RNA PCR Primer, Index 29 (96% over 26bp) |
| AATGACTGTCTCTTATACACATCTGACGCCACTAACCTCGTATGCCGTCTT | 11623 | 1.111186530771062 | No Hit |
| AATGATCTGTCTCTTATACACATCTGACGCCACTAACCTCGTATGCCGTCT | 9835 | 0.9402494648656452 | No Hit |
| AACTGTCTCTTATACACATCTGACGCCACTAACCTCGTATGCCGTCTTCTG | 8548 | 0.8172091942726523 | RNA PCR Primer, Index 29 (95% over 24bp) |
| AATGATACGGCGACTGTCTCTTATACACATCTGACGCCACTAACCTCGTAT | 7060 | 0.674952844123178 | No Hit |
| AATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCCACTAACCTC | 6917 | 0.6612817029461787 | No Hit |
| AATGCTGTCTCTTATACACATCTGACGCCACTAACCTCGTATGCCGTCTTC | 3447 | 0.32954142403577824 | No Hit |
| AATGATACGGCGCTGTCTCTTATACACATCTGACGCCACTAACCTCGTATG | 2050 | 0.1959848909989398 | No Hit |
| AATGATACGGCCTGTCTCTTATACACATCTGACGCCACTAACCTCGTATGC | 1866 | 0.17839405200196176 | No Hit |
| AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA | 1675 | 0.16013399630401176 | No Hit |
| AATGATACGGCGACCTGTCTCTTATACACATCTGACGCCACTAACCTCGTA | 1649 | 0.1576483342718301 | No Hit |
| AATGATACGGCGACCACCCTGTCTCTTATACACATCTGACGCCACTAACCT | 1242 | 0.11873816322960157 | No Hit |
| CCTGTCTCTTATACACATCTGACGCCACTAACCTCGTATGCCGTCTTCTGC | 1193 | 0.11405364632279763 | RNA PCR Primer, Index 29 (96% over 25bp) |
| AAACTGTCTCTTATACACATCTGACGCCACTAACCTCGTATGCCGTCTTCT | 1159 | 0.11080316520379083 | No Hit |
Adapter Content
Kmer Content
| Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
|---|---|---|---|---|
| CTTCGCG | 40 | 6.8139343E-9 | 45.000004 | 1 |
| ATCTACG | 50 | 2.1827873E-11 | 45.000004 | 1 |
| ACGCACG | 50 | 2.1827873E-11 | 45.000004 | 1 |
| TATATCG | 20 | 7.0329936E-4 | 45.000004 | 24 |
| TTATCCG | 25 | 3.8905633E-5 | 45.000004 | 1 |
| CCGTCTA | 20 | 7.0329936E-4 | 45.000004 | 40 |
| CGTTACG | 20 | 7.0329936E-4 | 45.000004 | 1 |
| TACGCTA | 25 | 3.8905633E-5 | 45.000004 | 11 |
| ATATCGG | 20 | 7.0329936E-4 | 45.000004 | 2 |
| TGCGCTA | 20 | 7.0329936E-4 | 45.000004 | 31 |
| CCGTAGT | 40 | 6.8139343E-9 | 45.000004 | 27 |
| CGCTAAT | 20 | 7.0329936E-4 | 45.000004 | 19 |
| TCGTCTA | 20 | 7.0329936E-4 | 45.000004 | 24 |
| TATAGCG | 25 | 3.8905633E-5 | 45.000004 | 1 |
| CTCGAAG | 25 | 3.8905633E-5 | 45.000004 | 1 |
| TTACGCG | 25 | 3.8905633E-5 | 45.000004 | 1 |
| TACTACG | 40 | 6.8139343E-9 | 45.000004 | 1 |
| CGGTCGA | 20 | 7.0329936E-4 | 45.000004 | 25 |
| TCATACG | 40 | 6.8139343E-9 | 45.000004 | 1 |
| CGCACCG | 50 | 2.1827873E-11 | 45.000004 | 1 |