##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR3548417_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1045999 Sequences flagged as poor quality 0 Sequence length 51 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.446004250482076 31.0 31.0 34.0 30.0 34.0 2 31.662861054360473 31.0 31.0 34.0 30.0 34.0 3 31.72628080906387 31.0 31.0 34.0 30.0 34.0 4 35.39446118017321 37.0 35.0 37.0 33.0 37.0 5 35.07991881445393 37.0 35.0 37.0 32.0 37.0 6 35.0972285824365 37.0 35.0 37.0 32.0 37.0 7 34.94109650200431 37.0 35.0 37.0 32.0 37.0 8 35.26607004404402 37.0 35.0 37.0 33.0 37.0 9 37.042491436416285 39.0 37.0 39.0 33.0 39.0 10 36.49784846830637 38.0 35.0 39.0 32.0 39.0 11 36.547913525729946 38.0 35.0 39.0 32.0 39.0 12 36.27124978130954 38.0 35.0 39.0 32.0 39.0 13 36.50426338839712 38.0 35.0 39.0 32.0 39.0 14 37.38923555376248 39.0 36.0 41.0 32.0 41.0 15 37.62680461453596 39.0 36.0 41.0 32.0 41.0 16 37.20832046684557 39.0 36.0 41.0 31.0 41.0 17 37.19480037743822 39.0 36.0 40.0 31.0 41.0 18 36.93556494795884 39.0 36.0 40.0 31.0 41.0 19 36.89399511854218 39.0 35.0 40.0 30.0 41.0 20 37.24631954715062 39.0 36.0 40.0 32.0 41.0 21 37.370610296950574 39.0 36.0 40.0 32.0 41.0 22 37.301466827406145 39.0 36.0 40.0 32.0 41.0 23 37.13239018392943 39.0 36.0 40.0 31.0 41.0 24 37.062491455536765 39.0 36.0 40.0 31.0 41.0 25 37.095026859490304 39.0 36.0 40.0 31.0 41.0 26 36.98723325739317 39.0 36.0 40.0 31.0 41.0 27 36.95149421748969 39.0 36.0 40.0 31.0 41.0 28 36.87397024280138 39.0 36.0 40.0 31.0 41.0 29 36.726550407791976 39.0 35.0 40.0 30.0 41.0 30 37.018848010370945 39.0 36.0 40.0 31.0 41.0 31 36.729809493125714 39.0 36.0 40.0 30.0 41.0 32 36.54771084867194 39.0 35.0 40.0 30.0 41.0 33 36.61474341753673 39.0 35.0 40.0 30.0 41.0 34 36.546827482626654 39.0 35.0 40.0 30.0 41.0 35 36.68286872167182 39.0 35.0 40.0 30.0 41.0 36 36.555609517791126 39.0 35.0 40.0 30.0 41.0 37 36.6324910444465 39.0 35.0 40.0 30.0 41.0 38 36.41039618584721 39.0 35.0 40.0 30.0 41.0 39 36.09177255427586 38.0 35.0 40.0 30.0 41.0 40 35.950751386951616 38.0 35.0 40.0 29.0 41.0 41 36.10701539867629 38.0 35.0 40.0 30.0 41.0 42 36.28813985481822 39.0 35.0 40.0 30.0 41.0 43 36.152840490287275 38.0 35.0 40.0 30.0 41.0 44 36.20828891805824 38.0 35.0 40.0 30.0 41.0 45 35.8230246874041 38.0 35.0 40.0 29.0 41.0 46 35.42044973274353 38.0 34.0 40.0 27.0 41.0 47 35.54278350170507 38.0 34.0 40.0 28.0 41.0 48 35.560582753903205 38.0 34.0 40.0 28.0 41.0 49 35.471957430169624 38.0 34.0 40.0 27.0 41.0 50 35.47849950143356 38.0 34.0 40.0 27.0 41.0 51 33.65485244249756 37.0 32.0 40.0 22.0 41.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 8 1.0 9 0.0 10 1.0 11 2.0 12 5.0 13 7.0 14 12.0 15 21.0 16 54.0 17 145.0 18 269.0 19 704.0 20 1516.0 21 2865.0 22 3981.0 23 5005.0 24 5708.0 25 6374.0 26 7522.0 27 9339.0 28 12032.0 29 16332.0 30 22445.0 31 30978.0 32 42559.0 33 55588.0 34 74372.0 35 91118.0 36 107806.0 37 138672.0 38 199426.0 39 211091.0 40 49.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 14.677069480945967 47.42882163367269 15.701640250134083 22.192468635247263 2 17.309098765868804 46.0032944582165 16.414547241440957 20.27305953447374 3 16.227166565168798 23.236446688763564 38.81227419911492 21.724112546952725 4 32.7952512382899 23.68673392613186 17.13930892859362 26.378705906984617 5 14.956419652408846 42.60912295327242 18.875639460458373 23.558817933860357 6 20.04629067523009 27.26933773359248 33.35997453152441 19.32439705965302 7 65.16468944998991 17.912158615830418 10.459187819491223 6.463964114688446 8 67.4636400226004 2.9798307646565627 6.384136122501073 23.17239309024196 9 70.90427428706911 6.333371255613055 13.276207721039887 9.48614673627795 10 31.42899754206266 34.95787280867381 16.414834048598518 17.19829560066501 11 20.26292568157331 21.18931280049025 33.83464037728526 24.713121140651186 12 21.699064721859198 19.8822369811061 42.82575795961564 15.592940337419062 13 23.294094927433008 27.992760987343203 25.69438402904783 23.018760056175964 14 14.393225997347988 24.13874200644551 41.43302240250708 20.03500959369942 15 13.623053176915084 29.616567511058804 25.677175599594264 31.083203712431846 16 16.446956450245175 27.921728414654318 34.04945893829726 21.581856196803248 17 14.473723206236336 28.21666177501126 30.693432785308588 26.61618223344382 18 16.3991552573186 28.48061996235178 26.994289669492993 28.125935110836625 19 16.991029628135397 30.537409691596263 35.4626534059784 17.008907274289935 20 19.243708645992967 35.275941946407215 26.734824794287565 18.745524613312252 21 16.915981755240683 25.188647407884712 30.572208960046808 27.3231618768278 22 17.98223516466077 32.12947622320863 29.105668361059617 20.782620251070984 23 21.98176097682694 26.012739973938793 22.784916620379178 29.220582428855096 24 14.454220319522292 39.50959800152772 24.283962030556435 21.752219648393545 25 17.99351624619144 23.685299890344062 28.489797791393684 29.831386072070813 26 24.015414928694963 30.397160991549704 21.17631087601422 24.411113203741113 27 15.227356813916648 25.29572208004023 31.386741287515573 28.09017981852755 28 21.29399741299944 23.636638275944815 24.95308312914257 30.116281181913173 29 16.776689079052655 37.58311432420107 25.330616950876628 20.309579645869643 30 21.297725906047713 22.398874186304194 22.589409741309506 33.71399016633859 31 22.0004034420683 30.689895497032023 25.515894374660014 21.793806686239662 32 17.213974391944927 29.63396714528408 20.548490008116644 32.60356845465435 33 20.759197666536966 28.34008445514766 22.954324048110944 27.946393830204425 34 14.65010960813538 29.20165315645617 23.241513615213783 32.90672362019467 35 14.543417345523274 25.162547956546806 22.261015545903966 38.03301915202596 36 15.053551676435637 30.127849070601403 32.29219148393067 22.526407769032282 37 16.292749801864055 29.62239925659585 20.033001943596503 34.05184899794359 38 19.97564051208462 29.499932600317972 29.595917395714526 20.92850949188288 39 15.623915510435479 28.878517092272553 26.506048284941 28.991519112350968 40 18.77171966703601 31.474791084886316 20.77640609599053 28.97708315208714 41 16.06789299033747 21.458529119052695 33.99687762607804 28.4767002645318 42 22.290174273589173 22.88931442573081 21.375450645746316 33.445060654933705 43 20.731090565096142 23.626122013500968 28.51943453100815 27.12335289039473 44 19.2279342523272 19.82439753766495 26.58941356540494 34.35825464460291 45 28.40882257057607 25.25184058493364 24.51120890172935 21.82812794276094 46 14.737681393576857 22.852985519106614 39.152331885594535 23.25700120172199 47 21.67420810153738 27.821537114280225 22.419046289719205 28.08520849446319 48 16.798773230184732 20.333002230403665 36.41294112135863 26.455283418052982 49 21.89265955321181 19.220668471002362 29.661500632409783 29.225171343376044 50 19.992275327223066 20.506042548797847 25.18243325280426 34.31924887117483 51 15.313494563570329 18.647054155883513 37.889806778017956 28.149644502528204 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 2062.0 1 1658.0 2 1254.0 3 882.5 4 511.0 5 545.5 6 580.0 7 643.5 8 707.0 9 738.0 10 769.0 11 1005.0 12 1241.0 13 1260.0 14 1279.0 15 1350.5 16 1422.0 17 1713.0 18 2004.0 19 2029.5 20 2055.0 21 2537.0 22 3019.0 23 3929.0 24 4839.0 25 5139.0 26 6961.0 27 8483.0 28 9962.0 29 11441.0 30 12847.5 31 14254.0 32 17056.5 33 19859.0 34 23147.0 35 26435.0 36 28821.0 37 31207.0 38 32070.5 39 32934.0 40 35022.5 41 37111.0 42 42685.5 43 48260.0 44 117232.0 45 186204.0 46 171613.5 47 157023.0 48 136176.0 49 115329.0 50 100800.5 51 86272.0 52 75212.5 53 64153.0 54 56311.0 55 48469.0 56 41463.5 57 34458.0 58 31640.0 59 28822.0 60 25775.0 61 22728.0 62 19811.0 63 16894.0 64 13804.0 65 10714.0 66 8270.0 67 5826.0 68 4689.5 69 3553.0 70 3112.0 71 2671.0 72 2271.5 73 1872.0 74 1654.5 75 1320.5 76 1204.0 77 859.5 78 515.0 79 522.5 80 530.0 81 313.0 82 96.0 83 68.0 84 40.0 85 24.5 86 9.0 87 8.0 88 7.0 89 5.0 90 3.0 91 2.0 92 1.0 93 2.0 94 3.0 95 2.0 96 1.0 97 0.5 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 44 0.0 45 0.0 46 0.0 47 0.0 48 0.0 49 0.0 50 0.0 51 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 51 1045999.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 23.477243677226593 #Duplication Level Percentage of deduplicated Percentage of total 1 74.27241763954142 17.43711647420256 2 10.89569131857159 5.1160160023589505 3 4.134289088108994 2.911851370609013 4 2.0375409079778457 1.9134337759565363 5 1.2339320389064758 1.448466157927219 6 0.8064566349303186 1.1360027360025153 7 0.6085561027242938 1.0001053940445108 8 0.47752081691727805 0.8968698063772194 9 0.39217706954368414 0.828651296366794 >10 3.9574197171280945 21.096999597905562 >50 0.8785303035545566 14.351229095716656 >100 0.2914711595196846 10.064630118440153 >500 0.006998601287902031 1.1099479730744983 >1k 0.003293459429600956 1.3802827415079162 >5k 0.001646729714800478 3.127648590098464 >10k+ 0.0020584121435005976 16.180748869411442 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source AATGATACGGCTGTCTCTTATACACATCTGACGCCACTAACCTCGTATGCC 49001 4.684612509189779 No Hit AATGATACCTGTCTCTTATACACATCTGACGCCACTAACCTCGTATGCCGT 48707 4.656505407748956 No Hit AATCTGTCTCTTATACACATCTGACGCCACTAACCTCGTATGCCGTCTTCT 44768 4.2799276098734325 TruSeq Adapter, Index 12 (95% over 21bp) CTGTCTCTTATACACATCTGACGCCACTAACCTCGTATGCCGTCTTCTGCT 13314 1.2728501652487239 RNA PCR Primer, Index 29 (96% over 26bp) AATGACTGTCTCTTATACACATCTGACGCCACTAACCTCGTATGCCGTCTT 11623 1.111186530771062 No Hit AATGATCTGTCTCTTATACACATCTGACGCCACTAACCTCGTATGCCGTCT 9835 0.9402494648656452 No Hit AACTGTCTCTTATACACATCTGACGCCACTAACCTCGTATGCCGTCTTCTG 8548 0.8172091942726523 RNA PCR Primer, Index 29 (95% over 24bp) AATGATACGGCGACTGTCTCTTATACACATCTGACGCCACTAACCTCGTAT 7060 0.674952844123178 No Hit AATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCCACTAACCTC 6917 0.6612817029461787 No Hit AATGCTGTCTCTTATACACATCTGACGCCACTAACCTCGTATGCCGTCTTC 3447 0.32954142403577824 No Hit AATGATACGGCGCTGTCTCTTATACACATCTGACGCCACTAACCTCGTATG 2050 0.1959848909989398 No Hit AATGATACGGCCTGTCTCTTATACACATCTGACGCCACTAACCTCGTATGC 1866 0.17839405200196176 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1675 0.16013399630401176 No Hit AATGATACGGCGACCTGTCTCTTATACACATCTGACGCCACTAACCTCGTA 1649 0.1576483342718301 No Hit AATGATACGGCGACCACCCTGTCTCTTATACACATCTGACGCCACTAACCT 1242 0.11873816322960157 No Hit CCTGTCTCTTATACACATCTGACGCCACTAACCTCGTATGCCGTCTTCTGC 1193 0.11405364632279763 RNA PCR Primer, Index 29 (96% over 25bp) AAACTGTCTCTTATACACATCTGACGCCACTAACCTCGTATGCCGTCTTCT 1159 0.11080316520379083 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 9.560238585314135E-5 0.0 0.0 1.465010960813538 0.0 2 9.560238585314135E-5 0.0 0.0 1.8008621423156237 0.0 3 9.560238585314135E-5 0.0 0.0 3.0824121246769836 0.0 4 9.560238585314135E-5 0.0 0.0 9.26578323688646 0.0 5 9.560238585314135E-5 0.0 0.0 9.855841162372048 0.0 6 9.560238585314135E-5 0.0 0.0 12.18892178673211 0.0 7 9.560238585314135E-5 0.0 0.0 14.050300239292772 0.0 8 9.560238585314135E-5 0.0 0.0 14.823054324143714 0.0 9 9.560238585314135E-5 0.0 0.0 20.835488370447774 0.0 10 9.560238585314135E-5 0.0 0.0 22.297057645370597 0.0 11 9.560238585314135E-5 0.0 0.0 28.702130690373508 0.0 12 9.560238585314135E-5 0.0 0.0 29.619913594563666 0.0 13 9.560238585314135E-5 0.0 0.0 30.203088148267828 0.0 14 9.560238585314135E-5 0.0 0.0 31.26475264316696 0.0 15 9.560238585314135E-5 0.0 0.0 31.715231085306964 0.0 16 9.560238585314135E-5 0.0 0.0 32.33215328121729 0.0 17 9.560238585314135E-5 0.0 0.0 33.09114062250538 0.0 18 9.560238585314135E-5 0.0 0.0 34.87880963557326 0.0 19 9.560238585314135E-5 0.0 0.0 35.442098893019974 0.0 20 9.560238585314135E-5 0.0 0.0 35.90930775268428 0.0 21 9.560238585314135E-5 0.0 0.0 36.48703297039481 0.0 22 9.560238585314135E-5 0.0 0.0 37.04860138489616 0.0 23 9.560238585314135E-5 0.0 0.0 37.58665161247764 0.0 24 9.560238585314135E-5 0.0 0.0 37.95319115983858 0.0 25 9.560238585314135E-5 0.0 0.0 38.269061442697364 0.0 26 9.560238585314135E-5 0.0 0.0 38.582350461138105 0.0 27 9.560238585314135E-5 0.0 0.0 38.94296266057616 0.0 28 9.560238585314135E-5 0.0 0.0 39.277475408676295 0.0 29 9.560238585314135E-5 0.0 0.0 39.64305893217871 0.0 30 9.560238585314135E-5 0.0 0.0 40.211606320847345 0.0 31 9.560238585314135E-5 0.0 0.0 40.56265828170008 0.0 32 9.560238585314135E-5 0.0 0.0 40.904341208739204 0.0 33 9.560238585314135E-5 0.0 0.0 41.235507873334484 0.0 34 9.560238585314135E-5 0.0 0.0 41.552525384823504 0.0 35 9.560238585314135E-5 0.0 0.0 41.936751373567276 0.0 36 9.560238585314135E-5 0.0 0.0 42.2645719546577 0.0 37 9.560238585314135E-5 0.0 0.0 42.60329120773538 0.0 38 9.560238585314135E-5 0.0 0.0 42.94220166558477 0.0 39 9.560238585314135E-5 0.0 0.0 43.27642760652735 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CTTCGCG 40 6.8139343E-9 45.000004 1 ATCTACG 50 2.1827873E-11 45.000004 1 ACGCACG 50 2.1827873E-11 45.000004 1 TATATCG 20 7.0329936E-4 45.000004 24 TTATCCG 25 3.8905633E-5 45.000004 1 CCGTCTA 20 7.0329936E-4 45.000004 40 CGTTACG 20 7.0329936E-4 45.000004 1 TACGCTA 25 3.8905633E-5 45.000004 11 ATATCGG 20 7.0329936E-4 45.000004 2 TGCGCTA 20 7.0329936E-4 45.000004 31 CCGTAGT 40 6.8139343E-9 45.000004 27 CGCTAAT 20 7.0329936E-4 45.000004 19 TCGTCTA 20 7.0329936E-4 45.000004 24 TATAGCG 25 3.8905633E-5 45.000004 1 CTCGAAG 25 3.8905633E-5 45.000004 1 TTACGCG 25 3.8905633E-5 45.000004 1 TACTACG 40 6.8139343E-9 45.000004 1 CGGTCGA 20 7.0329936E-4 45.000004 25 TCATACG 40 6.8139343E-9 45.000004 1 CGCACCG 50 2.1827873E-11 45.000004 1 >>END_MODULE