FastQCFastQC Report
Sat 18 Jun 2016
SRR3548414_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3548414_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences148498
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA18691.2586028094654473No Hit
AATGATACCTGTCTCTTATACACATCTGACGCTCACCGTATCGTATGCCGT12710.8559037832159355No Hit
AATGATACGGCTGTCTCTTATACACATCTGACGCTCACCGTATCGTATGCC10820.7286293418093173No Hit
AATCTGTCTCTTATACACATCTGACGCTCACCGTATCGTATGCCGTCTTCT9550.643106304462013No Hit
CTGTCTCTTATACACATCTGACGCTCACCGTATCGTATGCCGTCTTCTGCT8160.5495023502000027Illumina Paired End PCR Primer 2 (95% over 21bp)
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4470.3010141550727956No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3920.2639766192137268No Hit
AAACTGTCTCTTATACACATCTGACGCTCACCGTATCGTATGCCGTCTTCT3200.21549111772549123TruSeq Adapter, Index 14 (95% over 21bp)
AATGACTGTCTCTTATACACATCTGACGCTCACCGTATCGTATGCCGTCTT3120.21010383978235397No Hit
AATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCTCACCGTATC2600.17508653315196163No Hit
CGCAGCGGGAGAAACAAGCGAGATAGGAATGTCTTACACGCGGGGCAAGAC2560.172392894180393No Hit
AAAAACTGTCTCTTATACACATCTGACGCTCACCGTATCGTATGCCGTCTT2550.17171948443750085No Hit
AATGATCTGTCTCTTATACACATCTGACGCTCACCGTATCGTATGCCGTCT2450.16498538700857923No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2320.15623106035098114No Hit
AATGATACGGCGACTGTCTCTTATACACATCTGACGCTCACCGTATCGTAT2130.14343627523603011No Hit
GCATTGGGAGAAACAAGCGAGATAGGAATGTCTTACACGCGGGGCAAGACA2110.14208945575024579No Hit
AACTGTCTCTTATACACATCTGACGCTCACCGTATCGTATGCCGTCTTCTG2020.13602876806421635TruSeq Adapter, Index 14 (95% over 22bp)
CCTGTCTCTTATACACATCTGACGCTCACCGTATCGTATGCCGTCTTCTGC1880.1266010316637261TruSeq Adapter, Index 14 (95% over 23bp)
ATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCATA1830.1232339829492653No Hit
AAAAAACTGTCTCTTATACACATCTGACGCTCACCGTATCGTATGCCGTCT1740.11717329526323586No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACACGAC600.045.00000426
CATGCGG302.152703E-645.0000042
GACACGA650.045.00000425
CCCTACA302.152703E-645.00000427
TGCGGGC650.045.0000044
CGCAAAG302.152703E-645.0000041
GACTAAT302.152703E-645.0000049
GCATAGG302.152703E-645.0000042
CGGACAC207.0134306E-445.025
AGCACAT207.0134306E-445.020
TCACGCG351.2023884E-745.01
TTCTATC207.0134306E-445.011
ACAACGG351.2023884E-745.02
ATCCTGC207.0134306E-445.020
ATCCTAA351.2023884E-745.025
TAATATC406.7466317E-945.021
CAGCGCG207.0134306E-445.01
TCCGCTA207.0134306E-445.033
CTCGTCC351.2023884E-745.037
CTCCGGT207.0134306E-445.022
Produced by FastQC (version 0.11.5)