##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3548414_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	148498
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.74042074640736	31.0	31.0	34.0	30.0	34.0
2	31.92412692426834	33.0	31.0	34.0	30.0	34.0
3	32.07987986370187	34.0	31.0	34.0	30.0	34.0
4	35.752663335533136	37.0	35.0	37.0	35.0	37.0
5	35.49439049684171	37.0	35.0	37.0	33.0	37.0
6	34.90560815633881	37.0	35.0	37.0	32.0	37.0
7	35.12408921332274	37.0	35.0	37.0	32.0	37.0
8	35.49098304354268	37.0	35.0	37.0	33.0	37.0
9	37.07041845681423	39.0	37.0	39.0	33.0	39.0
10	36.83844226858274	39.0	37.0	39.0	32.0	39.0
11	36.88278630015219	39.0	37.0	39.0	33.0	39.0
12	36.72285822031273	39.0	35.0	39.0	32.0	39.0
13	36.7155853950895	39.0	35.0	39.0	32.0	39.0
14	37.82568115395494	40.0	37.0	41.0	33.0	41.0
15	37.88923083139167	40.0	37.0	41.0	33.0	41.0
16	37.74760602836402	40.0	36.0	41.0	33.0	41.0
17	37.70805667416396	39.0	36.0	41.0	33.0	41.0
18	37.563105227006425	39.0	36.0	41.0	33.0	41.0
19	37.441824132311545	39.0	35.0	41.0	32.0	41.0
20	37.37214642621449	39.0	35.0	41.0	32.0	41.0
21	37.35015286401163	39.0	35.0	41.0	32.0	41.0
22	37.288024081132406	39.0	35.0	41.0	32.0	41.0
23	37.09584640870584	39.0	35.0	41.0	32.0	41.0
24	36.99362280973481	39.0	35.0	41.0	31.0	41.0
25	36.99395951460626	39.0	35.0	41.0	31.0	41.0
26	36.968571967299226	39.0	35.0	41.0	31.0	41.0
27	36.851351533353984	39.0	35.0	41.0	31.0	41.0
28	36.67048041051058	39.0	35.0	41.0	31.0	41.0
29	36.38625436032809	39.0	35.0	40.0	30.0	41.0
30	36.43106977871756	39.0	35.0	40.0	30.0	41.0
31	36.28009804845856	39.0	35.0	40.0	30.0	41.0
32	36.11491063852712	38.0	35.0	40.0	30.0	41.0
33	35.80104782555994	38.0	35.0	40.0	28.0	41.0
34	35.76277121577395	38.0	35.0	40.0	28.0	41.0
35	35.69545717787445	38.0	35.0	40.0	28.0	41.0
36	35.61944268609678	38.0	35.0	40.0	27.0	41.0
37	35.490430847553505	38.0	35.0	40.0	27.0	41.0
38	35.23854193322469	38.0	34.0	40.0	26.0	41.0
39	35.106701773761266	38.0	34.0	40.0	25.0	41.0
40	34.91913022397608	38.0	34.0	40.0	24.0	41.0
41	34.802246494902285	38.0	34.0	40.0	24.0	41.0
42	34.78001723928942	38.0	34.0	40.0	24.0	41.0
43	34.689551374429286	38.0	34.0	40.0	24.0	41.0
44	34.500222225215154	37.0	34.0	40.0	23.0	41.0
45	34.20035286670527	37.0	33.0	40.0	23.0	41.0
46	33.96408032431413	37.0	33.0	40.0	22.0	41.0
47	33.817236595779065	36.0	33.0	40.0	22.0	41.0
48	33.668002262656735	36.0	33.0	40.0	21.0	41.0
49	33.541973629274466	36.0	33.0	40.0	20.0	41.0
50	33.40165524114803	36.0	33.0	40.0	20.0	41.0
51	32.02225619200259	35.0	30.0	39.0	15.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	1.0
10	1.0
11	3.0
12	2.0
13	4.0
14	7.0
15	13.0
16	21.0
17	63.0
18	141.0
19	273.0
20	477.0
21	735.0
22	997.0
23	1241.0
24	1468.0
25	1649.0
26	1773.0
27	1979.0
28	2176.0
29	2672.0
30	3247.0
31	4081.0
32	5212.0
33	7039.0
34	10287.0
35	13706.0
36	14270.0
37	19217.0
38	28066.0
39	27674.0
40	2.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	21.52217538283344	35.75738393783081	16.37328448867998	26.347156190655767
2	24.06699080122291	35.642904281539145	16.527495319802288	23.762609597435656
3	18.196204662689063	33.53109132782933	21.586149308408196	26.68655470107342
4	21.570660884321676	34.273862274239384	18.342334576896658	25.813142264542282
5	18.72685154008808	37.072553165699205	17.754447871351804	26.446147422860918
6	18.905305121954505	38.04832388314994	20.835297445083437	22.21107354981212
7	75.70135624722218	12.680305458659378	6.7771956524667	4.841142641651739
8	77.14110627752562	9.654675483844901	4.990639604573799	8.213578634055677
9	72.18279034061065	13.049333997764279	9.265444652453231	5.502431009171841
10	31.6354429015879	37.13720050101685	14.124095947420168	17.103260649975084
11	26.402375789572925	33.05768427857615	22.42925830650918	18.110681625341755
12	24.883163409608212	27.898018828536415	29.737100836374903	17.481716925480477
13	25.244784441541302	30.57549596627564	23.200985871863594	20.978733720319465
14	19.06961709922019	32.99977104068741	27.711484329755283	20.219127530337108
15	17.281040822098614	32.55330038114991	27.11147624883837	23.0541825479131
16	18.987461110587347	33.69068943689477	26.083179571442038	21.23866988107584
17	19.14369217093833	30.602432355991326	25.338388395803314	24.915487077267034
18	23.209740198521192	29.59299115139598	23.32421985481286	23.87304879526997
19	22.244744036956725	31.413217686433487	25.386200487548656	20.955837789061132
20	24.09729423965306	30.361351668035937	22.846772347102316	22.69458174520869
21	22.325553206103784	31.219949090223437	23.353849883500114	23.10064782017266
22	22.533636816657463	31.693356139476624	22.58548936686016	23.18751767700575
23	22.017131543859175	31.788980322967316	22.51074088539913	23.68314724777438
24	18.440652399358914	31.621301296987163	25.275087879971448	24.662958423682475
25	20.998936012606233	32.2879769424504	23.775404382550608	22.93768266239276
26	23.498632978221927	30.64418376005064	21.718137618015056	24.13904564371237
27	20.524182143867257	30.81455642500236	22.986841573623888	25.674419857506496
28	21.250117846705006	31.085267141645005	25.291923123543754	22.372691888106235
29	20.498592573637357	33.36677935056364	22.079758649948147	24.054869425850853
30	21.694568277013833	30.109496424194266	24.310765128149875	23.885170170642027
31	23.29324300663982	30.864388745976377	21.368637961454027	24.473730285929776
32	21.97133968134251	28.627321580088623	23.011757734110898	26.389581004457973
33	21.60635160069496	29.2131880564048	23.391560829102076	25.788899513798164
34	18.59418982073833	27.68522134978249	27.42259155005455	26.29799727942464
35	20.464248676749854	30.18626513488397	24.846799283492032	24.502686904874142
36	22.024539051030988	29.79232043529206	24.80235424046115	23.3807862732158
37	21.623186844267263	29.847540034209214	21.695241686756724	26.834031434766796
38	23.97001979824644	29.600398658567794	23.0030034074533	23.426578135732466
39	23.729612520033943	24.905385931123654	25.33165429837439	26.03334725046802
40	27.163328799041064	26.508101119206994	24.58080243504963	21.74776764670231
41	20.015757787983677	26.434699457231748	28.039434874543765	25.510107880240813
42	25.28586243585772	27.571415103233715	23.647456531401094	23.495265929507468
43	23.204352920578057	25.262293094856496	25.90876644803297	25.624587536532477
44	24.993602607442526	24.48450484181605	24.729625988228797	25.792266562512626
45	26.050855903783216	24.473730285929776	24.42457137469865	25.05084243558836
46	20.604991313014317	25.40303573112096	28.5088014653396	25.483171490525123
47	22.640708965777318	28.172770003636412	24.67710002828321	24.50942100230306
48	21.82251612816334	25.960618998235667	28.745168285094746	23.47169658850624
49	23.753855270778057	25.71819149079449	25.95186467157807	24.576088566849386
50	20.934962087031476	24.71750461285674	26.84682621988175	27.500707080230036
51	20.7181241498202	24.464302549529286	28.425972066963865	26.39160123368665
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	2216.0
1	1754.0
2	1292.0
3	870.0
4	448.0
5	420.5
6	393.0
7	411.5
8	430.0
9	476.0
10	522.0
11	578.5
12	635.0
13	658.5
14	682.0
15	763.5
16	845.0
17	939.0
18	1033.0
19	882.5
20	732.0
21	739.5
22	747.0
23	817.0
24	887.0
25	936.5
26	1089.0
27	1192.0
28	1277.0
29	1362.0
30	1652.5
31	1943.0
32	2380.0
33	2817.0
34	2569.0
35	2321.0
36	2812.5
37	3304.0
38	3666.0
39	4028.0
40	4309.5
41	4591.0
42	5032.5
43	5474.0
44	8116.5
45	10759.0
46	11261.5
47	11764.0
48	12269.5
49	12775.0
50	12804.5
51	12834.0
52	12215.0
53	11596.0
54	10552.0
55	9508.0
56	8926.0
57	8344.0
58	7738.0
59	7132.0
60	6706.0
61	6280.0
62	5782.5
63	5285.0
64	4392.5
65	3500.0
66	3023.0
67	2546.0
68	2346.0
69	2146.0
70	1888.0
71	1630.0
72	1385.5
73	1141.0
74	1045.5
75	835.0
76	720.0
77	533.0
78	346.0
79	260.0
80	174.0
81	159.5
82	145.0
83	87.5
84	30.0
85	17.0
86	4.0
87	3.0
88	2.0
89	2.0
90	2.0
91	2.0
92	2.0
93	2.0
94	2.0
95	1.5
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	148498.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	36.997804684238176
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.93045266740685	29.94249080795701
2	7.200451393312826	5.328017885762771
3	2.517245772738028	2.793977023259573
4	1.2959356400502358	1.9178709477568723
5	0.9810524016672431	1.8148392570943714
6	0.6625288946324238	1.4707268784764778
7	0.5169181485593637	1.3387385688696143
8	0.4277315665896143	1.2660103166372612
9	0.382228208441783	1.2727444140661828
>10	4.748730456307675	39.14463494457838
>50	0.2875812234942939	6.536788374254199
>100	0.04004295517009155	3.1374159921345743
>500	0.003640268651826505	1.1926086546620156
>1k	0.005460402977739757	2.8431359344907
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1869	1.2586028094654473	No Hit
AATGATACCTGTCTCTTATACACATCTGACGCTCACCGTATCGTATGCCGT	1271	0.8559037832159355	No Hit
AATGATACGGCTGTCTCTTATACACATCTGACGCTCACCGTATCGTATGCC	1082	0.7286293418093173	No Hit
AATCTGTCTCTTATACACATCTGACGCTCACCGTATCGTATGCCGTCTTCT	955	0.643106304462013	No Hit
CTGTCTCTTATACACATCTGACGCTCACCGTATCGTATGCCGTCTTCTGCT	816	0.5495023502000027	Illumina Paired End PCR Primer 2 (95% over 21bp)
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	447	0.3010141550727956	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	392	0.2639766192137268	No Hit
AAACTGTCTCTTATACACATCTGACGCTCACCGTATCGTATGCCGTCTTCT	320	0.21549111772549123	TruSeq Adapter, Index 14 (95% over 21bp)
AATGACTGTCTCTTATACACATCTGACGCTCACCGTATCGTATGCCGTCTT	312	0.21010383978235397	No Hit
AATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCTCACCGTATC	260	0.17508653315196163	No Hit
CGCAGCGGGAGAAACAAGCGAGATAGGAATGTCTTACACGCGGGGCAAGAC	256	0.172392894180393	No Hit
AAAAACTGTCTCTTATACACATCTGACGCTCACCGTATCGTATGCCGTCTT	255	0.17171948443750085	No Hit
AATGATCTGTCTCTTATACACATCTGACGCTCACCGTATCGTATGCCGTCT	245	0.16498538700857923	No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	232	0.15623106035098114	No Hit
AATGATACGGCGACTGTCTCTTATACACATCTGACGCTCACCGTATCGTAT	213	0.14343627523603011	No Hit
GCATTGGGAGAAACAAGCGAGATAGGAATGTCTTACACGCGGGGCAAGACA	211	0.14208945575024579	No Hit
AACTGTCTCTTATACACATCTGACGCTCACCGTATCGTATGCCGTCTTCTG	202	0.13602876806421635	TruSeq Adapter, Index 14 (95% over 22bp)
CCTGTCTCTTATACACATCTGACGCTCACCGTATCGTATGCCGTCTTCTGC	188	0.1266010316637261	TruSeq Adapter, Index 14 (95% over 23bp)
ATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCATA	183	0.1232339829492653	No Hit
AAAAAACTGTCTCTTATACACATCTGACGCTCACCGTATCGTATGCCGTCT	174	0.11717329526323586	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	6.734097428921601E-4	0.0	0.0	0.6565744993198561	0.0
2	6.734097428921601E-4	0.0	0.0	0.9697100297647107	0.0
3	6.734097428921601E-4	0.0	0.0	1.4619725518188798	0.0
4	6.734097428921601E-4	0.0	0.0	3.5677248178426644	0.0
5	6.734097428921601E-4	0.0	0.0	3.9192447036323723	0.0
6	6.734097428921601E-4	0.0	0.0	5.43980390308287	0.0
7	6.734097428921601E-4	0.0	0.0	6.6128836752010125	0.0
8	6.734097428921601E-4	0.0	0.0	7.469460868159841	0.0
9	6.734097428921601E-4	0.0	0.0	9.270831930396369	0.0
10	6.734097428921601E-4	0.0	0.0	10.841896860563779	0.0
11	6.734097428921601E-4	0.0	0.0	12.76582849600668	0.0
12	6.734097428921601E-4	0.0	0.0	13.474928955272125	0.0
13	6.734097428921601E-4	0.0	0.0	13.883015259464774	0.0
14	6.734097428921601E-4	0.0	0.0	14.338913655402767	0.0
15	6.734097428921601E-4	0.0	0.0	14.68571967299223	0.0
16	6.734097428921601E-4	0.0	0.0	15.320071650796644	0.0
17	6.734097428921601E-4	0.0	0.0	15.909305175827283	0.0
18	6.734097428921601E-4	0.0	0.0	17.147032283263073	0.0
19	6.734097428921601E-4	0.0	0.0	17.46959555010842	0.0
20	6.734097428921601E-4	0.0	0.0	18.039300192595185	0.0
21	6.734097428921601E-4	0.0	0.0	18.480383574189553	0.0
22	6.734097428921601E-4	0.0	0.0	18.89385715632534	0.0
23	6.734097428921601E-4	0.0	0.0	19.25682500774421	0.0
24	6.734097428921601E-4	0.0	0.0	19.550431655645195	0.0
25	6.734097428921601E-4	0.0	0.0	19.806327357944216	0.0
26	6.734097428921601E-4	0.0	0.0	20.040000538727796	0.0
27	6.734097428921601E-4	0.0	0.0	20.421150453204756	0.0
28	6.734097428921601E-4	0.0	0.0	20.687820711390053	0.0
29	0.0013468194857843203	0.0	0.0	20.944389823431965	0.0
30	0.0013468194857843203	0.0	0.0	21.516114695147408	0.0
31	0.0013468194857843203	0.0	0.0	21.749114466188097	0.0
32	0.0013468194857843203	0.0	0.0	22.034640197174372	0.0
33	0.0013468194857843203	0.0	0.0	22.296596587159424	0.0
34	0.0013468194857843203	0.0	0.0	22.528922948457218	0.0
35	0.0013468194857843203	0.0	0.0	22.7794313728131	0.0
36	0.0013468194857843203	0.0	0.0	23.006370456167758	0.0
37	0.0013468194857843203	0.0	0.0	23.250144783094722	0.0
38	0.0013468194857843203	0.0	0.0	23.48583819310698	0.0
39	0.0013468194857843203	0.0	0.0	23.738366846691537	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACACGAC	60	0.0	45.000004	26
CATGCGG	30	2.152703E-6	45.000004	2
GACACGA	65	0.0	45.000004	25
CCCTACA	30	2.152703E-6	45.000004	27
TGCGGGC	65	0.0	45.000004	4
CGCAAAG	30	2.152703E-6	45.000004	1
GACTAAT	30	2.152703E-6	45.000004	9
GCATAGG	30	2.152703E-6	45.000004	2
CGGACAC	20	7.0134306E-4	45.0	25
AGCACAT	20	7.0134306E-4	45.0	20
TCACGCG	35	1.2023884E-7	45.0	1
TTCTATC	20	7.0134306E-4	45.0	11
ACAACGG	35	1.2023884E-7	45.0	2
ATCCTGC	20	7.0134306E-4	45.0	20
ATCCTAA	35	1.2023884E-7	45.0	25
TAATATC	40	6.7466317E-9	45.0	21
CAGCGCG	20	7.0134306E-4	45.0	1
TCCGCTA	20	7.0134306E-4	45.0	33
CTCGTCC	35	1.2023884E-7	45.0	37
CTCCGGT	20	7.0134306E-4	45.0	22
>>END_MODULE