Basic Statistics
Measure | Value |
---|---|
Filename | SRR3548412_1.fastq |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 256750 |
Sequences flagged as poor quality | 0 |
Sequence length | 51 |
%GC | 49 |
Per base sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
CTGTCTCTTATACACATCTGACGCATAGCAGGTCGTATGCCGTCTTCTGCT | 1552 | 0.6044790652385589 | Illumina Single End Adapter 2 (95% over 22bp) |
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA | 1308 | 0.5094449853943525 | No Hit |
AATGATACGGCTGTCTCTTATACACATCTGACGCATAGCAGGTCGTATGCC | 901 | 0.35092502434274586 | No Hit |
AATGATACCTGTCTCTTATACACATCTGACGCATAGCAGGTCGTATGCCGT | 809 | 0.3150925024342746 | No Hit |
AAACTGTCTCTTATACACATCTGACGCATAGCAGGTCGTATGCCGTCTTCT | 694 | 0.27030185004868545 | No Hit |
AATCTGTCTCTTATACACATCTGACGCATAGCAGGTCGTATGCCGTCTTCT | 677 | 0.2636806231742941 | No Hit |
AAAAACTGTCTCTTATACACATCTGACGCATAGCAGGTCGTATGCCGTCTT | 549 | 0.21382667964946447 | No Hit |
TGCAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGG | 396 | 0.1542356377799416 | No Hit |
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA | 341 | 0.13281402142161636 | No Hit |
TACAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGG | 335 | 0.13047711781889 | No Hit |
CCTGTCTCTTATACACATCTGACGCATAGCAGGTCGTATGCCGTCTTCTGC | 323 | 0.1258033106134372 | Illumina Single End Adapter 2 (95% over 21bp) |
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA | 310 | 0.12074001947419669 | No Hit |
AAAAAACTGTCTCTTATACACATCTGACGCATAGCAGGTCGTATGCCGTCT | 267 | 0.10399221032132425 | No Hit |
GAAAAACTGTCTCTTATACACATCTGACGCATAGCAGGTCGTATGCCGTCT | 262 | 0.10204479065238559 | No Hit |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
TGATCGT | 20 | 7.023042E-4 | 45.000004 | 28 |
TTGATAT | 20 | 7.023042E-4 | 45.000004 | 37 |
CTTCGCG | 20 | 7.023042E-4 | 45.000004 | 28 |
TAGAGTC | 20 | 7.023042E-4 | 45.000004 | 33 |
TTCTACG | 20 | 7.023042E-4 | 45.000004 | 1 |
CCCCTAT | 20 | 7.023042E-4 | 45.000004 | 28 |
CGTATTT | 20 | 7.023042E-4 | 45.000004 | 34 |
ATTAGTG | 20 | 7.023042E-4 | 45.000004 | 24 |
GATCGTG | 20 | 7.023042E-4 | 45.000004 | 29 |
TTGTGTC | 30 | 2.1588949E-6 | 45.000004 | 39 |
CAAGTAT | 20 | 7.023042E-4 | 45.000004 | 42 |
ACCATTA | 20 | 7.023042E-4 | 45.000004 | 10 |
CCCTATA | 20 | 7.023042E-4 | 45.000004 | 29 |
TTAGCCA | 20 | 7.023042E-4 | 45.000004 | 39 |
TGGACGT | 20 | 7.023042E-4 | 45.000004 | 28 |
GACGTGA | 20 | 7.023042E-4 | 45.000004 | 30 |
TAGGTAT | 20 | 7.023042E-4 | 45.000004 | 19 |
TTAGAGT | 20 | 7.023042E-4 | 45.000004 | 32 |
TATGGGC | 30 | 2.1588949E-6 | 45.000004 | 4 |
GGCCGTA | 30 | 2.1588949E-6 | 45.000004 | 42 |