FastQCFastQC Report
Sat 18 Jun 2016
SRR3548412_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3548412_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences256750
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTCTCTTATACACATCTGACGCATAGCAGGTCGTATGCCGTCTTCTGCT15520.6044790652385589Illumina Single End Adapter 2 (95% over 22bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA13080.5094449853943525No Hit
AATGATACGGCTGTCTCTTATACACATCTGACGCATAGCAGGTCGTATGCC9010.35092502434274586No Hit
AATGATACCTGTCTCTTATACACATCTGACGCATAGCAGGTCGTATGCCGT8090.3150925024342746No Hit
AAACTGTCTCTTATACACATCTGACGCATAGCAGGTCGTATGCCGTCTTCT6940.27030185004868545No Hit
AATCTGTCTCTTATACACATCTGACGCATAGCAGGTCGTATGCCGTCTTCT6770.2636806231742941No Hit
AAAAACTGTCTCTTATACACATCTGACGCATAGCAGGTCGTATGCCGTCTT5490.21382667964946447No Hit
TGCAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGG3960.1542356377799416No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3410.13281402142161636No Hit
TACAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGG3350.13047711781889No Hit
CCTGTCTCTTATACACATCTGACGCATAGCAGGTCGTATGCCGTCTTCTGC3230.1258033106134372Illumina Single End Adapter 2 (95% over 21bp)
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3100.12074001947419669No Hit
AAAAAACTGTCTCTTATACACATCTGACGCATAGCAGGTCGTATGCCGTCT2670.10399221032132425No Hit
GAAAAACTGTCTCTTATACACATCTGACGCATAGCAGGTCGTATGCCGTCT2620.10204479065238559No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGATCGT207.023042E-445.00000428
TTGATAT207.023042E-445.00000437
CTTCGCG207.023042E-445.00000428
TAGAGTC207.023042E-445.00000433
TTCTACG207.023042E-445.0000041
CCCCTAT207.023042E-445.00000428
CGTATTT207.023042E-445.00000434
ATTAGTG207.023042E-445.00000424
GATCGTG207.023042E-445.00000429
TTGTGTC302.1588949E-645.00000439
CAAGTAT207.023042E-445.00000442
ACCATTA207.023042E-445.00000410
CCCTATA207.023042E-445.00000429
TTAGCCA207.023042E-445.00000439
TGGACGT207.023042E-445.00000428
GACGTGA207.023042E-445.00000430
TAGGTAT207.023042E-445.00000419
TTAGAGT207.023042E-445.00000432
TATGGGC302.1588949E-645.0000044
GGCCGTA302.1588949E-645.00000442