##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR3548412_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 256750 Sequences flagged as poor quality 0 Sequence length 51 %GC 49 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.78993962999026 31.0 31.0 34.0 30.0 34.0 2 31.98831158714703 33.0 31.0 34.0 30.0 34.0 3 32.147996105160665 34.0 31.0 34.0 30.0 34.0 4 35.814819863680626 37.0 35.0 37.0 35.0 37.0 5 35.57954040895813 37.0 35.0 37.0 33.0 37.0 6 34.92460370009737 37.0 35.0 37.0 33.0 37.0 7 35.313219084712756 37.0 35.0 37.0 33.0 37.0 8 35.70301071080818 37.0 35.0 37.0 35.0 37.0 9 37.34921908471276 39.0 37.0 39.0 34.0 39.0 10 36.95970788704966 39.0 37.0 39.0 33.0 39.0 11 36.994465433300874 39.0 37.0 39.0 33.0 39.0 12 36.883178188899706 39.0 37.0 39.0 33.0 39.0 13 36.86066212268744 39.0 37.0 39.0 33.0 39.0 14 38.02363777994158 40.0 37.0 41.0 33.0 41.0 15 38.03422005842259 40.0 37.0 41.0 33.0 41.0 16 37.950671859785786 40.0 37.0 41.0 33.0 41.0 17 37.91937682570594 40.0 37.0 41.0 33.0 41.0 18 37.90712366114898 40.0 37.0 41.0 33.0 41.0 19 37.880467380720546 40.0 37.0 41.0 33.0 41.0 20 37.7878558909445 40.0 36.0 41.0 33.0 41.0 21 37.75898344693282 40.0 36.0 41.0 33.0 41.0 22 37.7308899707887 40.0 36.0 41.0 33.0 41.0 23 37.59922103213243 39.0 36.0 41.0 33.0 41.0 24 37.495407984420645 39.0 36.0 41.0 32.0 41.0 25 37.524194741966895 39.0 36.0 41.0 32.0 41.0 26 37.4696864654333 39.0 36.0 41.0 33.0 41.0 27 37.355587147030185 39.0 36.0 41.0 32.0 41.0 28 37.238259006815966 39.0 36.0 41.0 32.0 41.0 29 37.024245374878284 39.0 35.0 41.0 31.0 41.0 30 37.0115988315482 39.0 35.0 41.0 31.0 41.0 31 36.98533592989289 39.0 35.0 41.0 31.0 41.0 32 36.8991665043817 39.0 35.0 41.0 31.0 41.0 33 36.63956767283349 39.0 35.0 41.0 30.0 41.0 34 36.600377799415774 39.0 35.0 41.0 30.0 41.0 35 36.57645959104187 39.0 35.0 41.0 30.0 41.0 36 36.46869717624148 39.0 35.0 40.0 30.0 41.0 37 36.44628237585199 39.0 35.0 40.0 30.0 41.0 38 36.22427263875365 39.0 35.0 40.0 30.0 41.0 39 36.211002921129506 39.0 35.0 40.0 30.0 41.0 40 36.13757740993184 38.0 35.0 40.0 30.0 41.0 41 36.08491139240506 38.0 35.0 40.0 30.0 41.0 42 35.976303797468354 38.0 35.0 40.0 30.0 41.0 43 35.879026290165534 38.0 35.0 40.0 29.0 41.0 44 35.78101655306718 38.0 35.0 40.0 29.0 41.0 45 35.591400194741965 38.0 35.0 40.0 29.0 41.0 46 35.40618500486855 38.0 34.0 40.0 28.0 41.0 47 35.329534566699124 38.0 34.0 40.0 28.0 41.0 48 35.20754819863681 37.0 34.0 40.0 27.0 41.0 49 35.09452385589095 37.0 34.0 40.0 27.0 41.0 50 34.96176436222006 37.0 34.0 40.0 27.0 41.0 51 33.809729308666014 36.0 32.0 39.0 23.0 41.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 9 2.0 10 1.0 11 2.0 12 2.0 13 3.0 14 10.0 15 16.0 16 30.0 17 52.0 18 152.0 19 256.0 20 451.0 21 719.0 22 951.0 23 1251.0 24 1416.0 25 1707.0 26 1979.0 27 2316.0 28 2756.0 29 3524.0 30 4575.0 31 6195.0 32 8143.0 33 10941.0 34 16534.0 35 21879.0 36 24924.0 37 35935.0 38 51793.0 39 58219.0 40 16.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 20.334956183057447 33.247127555988314 20.97176241480039 25.446153846153845 2 24.10282375851996 31.757351509250242 18.61304771178189 25.526777020447906 3 21.238948393378774 31.09250243427459 19.835637779941578 27.832911392405062 4 20.76689386562804 32.61148977604674 19.076923076923077 27.544693281402143 5 18.894644595910417 33.69737098344693 18.454527750730282 28.953456669912363 6 18.9713729308666 38.47439143135345 19.920155793573517 22.634079844206425 7 82.47984420642649 6.945277507302825 5.746056475170399 4.828821811100292 8 83.5240506329114 5.632716650438169 4.960467380720545 5.882765335929893 9 76.84985394352482 9.094839337877312 8.84985394352483 5.205452775073028 10 34.53203505355404 32.90944498539435 14.257059396299903 18.301460564751704 11 26.682765335929894 29.63700097370984 23.985589094449857 19.694644595910418 12 26.106329113924048 24.692891918208375 29.64673807205453 19.55404089581305 13 26.00077896786758 24.56864654333009 27.395520934761443 22.035053554040896 14 22.033885102239534 27.98364167478092 28.59084712755599 21.391626095423565 15 20.124634858812072 29.608568646543333 27.440311587147033 22.826484907497566 16 22.232521908471277 29.630769230769232 24.645374878286272 23.491333982473222 17 20.456085686465432 27.813826679649466 27.613242453748782 24.11684518013632 18 23.544693281402143 25.891333982473224 25.659201557935734 24.904771178188902 19 22.668354430379747 31.68958130477118 24.551509250243427 21.09055501460565 20 23.81421616358325 29.374488802336902 23.74060370009737 23.070691333982474 21 23.44537487828627 28.76923076923077 25.38266796494645 22.402726387536514 22 22.285881207400195 26.7333982473223 24.816358325219085 26.164362220058422 23 22.533592989289193 28.78948393378773 24.31236611489776 24.364556962025315 24 20.474391431353457 29.180136319376825 25.44732229795521 24.898149951314508 25 21.58792599805258 28.912950340798442 23.815384615384616 25.683739045764366 26 24.405842259006818 28.140603700097373 24.306523855890944 23.14703018500487 27 21.188704965920156 28.264849074975658 23.841480038948394 26.704965920155793 28 22.25589094449854 30.606426484907495 24.990847127555988 22.146835443037975 29 23.371372930866603 30.090749756572542 24.090749756572542 22.447127555988317 30 23.383446932814024 29.697760467380718 23.9205452775073 22.998247322297953 31 25.814216163583254 27.866796494644596 21.682960077896787 24.636027263875366 32 23.67361246348588 27.84381694255112 24.44089581304771 24.041674780915287 33 23.940408958130476 28.63641674780915 22.728724440116846 24.694449853943524 34 22.491918208373903 28.08101265822785 25.8157740993184 23.611295034079845 35 24.19629990262902 28.25744888023369 25.926777020447904 21.619474196689385 36 24.764946445959104 29.798247322297954 24.644595910418694 20.792210321324244 37 25.549756572541384 28.484907497565725 21.485881207400194 24.479454722492697 38 24.170204479065237 28.810905550146053 24.031158714703018 22.987731256085688 39 24.586562804284323 25.269328140214213 23.652190847127557 26.491918208373903 40 25.71879259980526 26.534371957156765 23.319182083739047 24.42765335929893 41 23.858227848101265 24.7396299902629 27.31567672833496 24.086465433300877 42 25.966504381694257 26.083739045764364 24.377020447906524 23.572736124634858 43 23.052775073028236 25.146251217137294 26.38987341772152 25.41110029211295 44 25.169230769230772 24.482960077896788 24.94800389483934 25.399805258033105 45 26.655890944498537 24.00155793573515 26.44829600778968 22.89425511197663 46 21.55326192794547 24.799221032132422 28.02414800389484 25.623369036027267 47 23.837585199610515 27.05043816942551 25.60973709834469 23.502239532619278 48 23.540408958130477 24.62122687439143 28.29367088607595 23.544693281402143 49 24.14255111976631 23.831353456669913 27.479259980525804 24.546835443037978 50 22.624342745861732 24.324050632911394 26.947224926971764 26.104381694255114 51 23.564556962025314 22.44673807205453 28.720155793573515 25.26854917234664 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1623.0 1 1328.5 2 1034.0 3 689.5 4 345.0 5 322.5 6 300.0 7 320.5 8 341.0 9 377.0 10 413.0 11 466.5 12 520.0 13 511.0 14 502.0 15 575.0 16 648.0 17 621.0 18 594.0 19 777.0 20 960.0 21 968.0 22 976.0 23 988.5 24 1001.0 25 1120.5 26 1346.5 27 1453.0 28 1615.0 29 1777.0 30 2506.5 31 3236.0 32 3445.0 33 3654.0 34 4871.5 35 6089.0 36 6415.5 37 6742.0 38 7068.0 39 7394.0 40 8208.5 41 9023.0 42 10945.5 43 12868.0 44 15237.5 45 17607.0 46 19781.5 47 21956.0 48 23487.5 49 25019.0 50 24340.5 51 23662.0 52 21349.5 53 19037.0 54 16849.0 55 14661.0 56 14002.5 57 13344.0 58 12353.0 59 11362.0 60 11381.5 61 11401.0 62 10280.5 63 9160.0 64 8366.5 65 7573.0 66 6976.5 67 6380.0 68 5464.5 69 4549.0 70 3763.5 71 2978.0 72 2361.5 73 1745.0 74 1471.5 75 1081.0 76 964.0 77 847.5 78 731.0 79 527.0 80 323.0 81 243.5 82 164.0 83 150.0 84 136.0 85 83.5 86 31.0 87 24.5 88 18.0 89 12.5 90 7.0 91 6.5 92 6.0 93 4.0 94 2.0 95 2.0 96 2.0 97 1.0 98 0.0 99 0.5 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 44 0.0 45 0.0 46 0.0 47 0.0 48 0.0 49 0.0 50 0.0 51 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 51 256750.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 29.466017526777023 #Duplication Level Percentage of deduplicated Percentage of total 1 78.08179342797472 23.007594936708863 2 9.247362994686336 5.449659201557936 3 3.1287175826790388 2.76572541382668 4 1.565019694926904 1.8445959104186953 5 0.9027943003674623 1.3300876338851022 6 0.6199275649668226 1.0960077896786757 7 0.46395431834403994 0.9569620253164556 8 0.3820022735083406 0.9004868549172348 9 0.28815396409971716 0.7641674780915287 >10 3.9284109234144924 27.863680623174293 >50 1.2187062151373356 24.890360272638752 >100 0.16390408967139874 6.602921129503408 >500 0.006609035873846723 1.4138266796494645 >1k 0.0026436143495386895 1.1139240506329113 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source CTGTCTCTTATACACATCTGACGCATAGCAGGTCGTATGCCGTCTTCTGCT 1552 0.6044790652385589 Illumina Single End Adapter 2 (95% over 22bp) AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1308 0.5094449853943525 No Hit AATGATACGGCTGTCTCTTATACACATCTGACGCATAGCAGGTCGTATGCC 901 0.35092502434274586 No Hit AATGATACCTGTCTCTTATACACATCTGACGCATAGCAGGTCGTATGCCGT 809 0.3150925024342746 No Hit AAACTGTCTCTTATACACATCTGACGCATAGCAGGTCGTATGCCGTCTTCT 694 0.27030185004868545 No Hit AATCTGTCTCTTATACACATCTGACGCATAGCAGGTCGTATGCCGTCTTCT 677 0.2636806231742941 No Hit AAAAACTGTCTCTTATACACATCTGACGCATAGCAGGTCGTATGCCGTCTT 549 0.21382667964946447 No Hit TGCAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGG 396 0.1542356377799416 No Hit CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 341 0.13281402142161636 No Hit TACAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGG 335 0.13047711781889 No Hit CCTGTCTCTTATACACATCTGACGCATAGCAGGTCGTATGCCGTCTTCTGC 323 0.1258033106134372 Illumina Single End Adapter 2 (95% over 21bp) GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 310 0.12074001947419669 No Hit AAAAAACTGTCTCTTATACACATCTGACGCATAGCAGGTCGTATGCCGTCT 267 0.10399221032132425 No Hit GAAAAACTGTCTCTTATACACATCTGACGCATAGCAGGTCGTATGCCGTCT 262 0.10204479065238559 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.689776046738072 0.0 2 0.0 0.0 0.0 0.9920155793573515 0.0 3 0.0 0.0 0.0 1.3916260954235637 0.0 4 0.0 0.0 0.0 3.0613437195715676 0.0 5 0.0 0.0 0.0 3.342161635832522 0.0 6 0.0 0.0 0.0 4.673417721518987 0.0 7 0.0 0.0 0.0 5.711781888997079 0.0 8 0.0 0.0 0.0 6.5628042843232715 0.0 9 0.0 0.0 0.0 7.807205452775073 0.0 10 0.0 0.0 0.0 9.484323271665044 0.0 11 0.0 0.0 0.0 10.97682570593963 0.0 12 0.0 0.0 0.0 11.66854917234664 0.0 13 0.0 0.0 0.0 11.968062317429405 0.0 14 0.0 0.0 0.0 12.321324245374878 0.0 15 0.0 0.0 0.0 12.581499513145083 0.0 16 0.0 0.0 0.0 13.181304771178189 0.0 17 0.0 0.0 0.0 13.873417721518987 0.0 18 0.0 0.0 0.0 15.054722492697175 0.0 19 0.0 0.0 0.0 15.434469328140214 0.0 20 0.0 0.0 0.0 15.905744888023369 0.0 21 0.0 0.0 0.0 16.29406037000974 3.8948393378773126E-4 22 0.0 0.0 0.0 16.68471275559883 3.8948393378773126E-4 23 0.0 0.0 0.0 17.090944498539436 3.8948393378773126E-4 24 0.0 0.0 0.0 17.38072054527751 3.8948393378773126E-4 25 0.0 0.0 0.0 17.63933787731256 3.8948393378773126E-4 26 0.0 0.0 0.0 17.897955209347614 3.8948393378773126E-4 27 0.0 0.0 0.0 18.195131450827652 3.8948393378773126E-4 28 0.0 0.0 0.0 18.49425511197663 3.8948393378773126E-4 29 0.0 0.0 0.0 18.770399221032132 3.8948393378773126E-4 30 0.0 0.0 0.0 19.22181110029211 3.8948393378773126E-4 31 0.0 0.0 0.0 19.483933787731257 3.8948393378773126E-4 32 0.0 0.0 0.0 19.803700097370985 3.8948393378773126E-4 33 0.0 0.0 0.0 20.10438169425511 3.8948393378773126E-4 34 0.0 0.0 0.0 20.385589094449855 3.8948393378773126E-4 35 0.0 0.0 0.0 20.66640701071081 3.8948393378773126E-4 36 0.0 0.0 0.0 20.93242453748783 3.8948393378773126E-4 37 0.0 0.0 0.0 21.180525803310612 3.8948393378773126E-4 38 0.0 0.0 0.0 21.45783836416748 3.8948393378773126E-4 39 0.0 0.0 0.0 21.74255111976631 3.8948393378773126E-4 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TGATCGT 20 7.023042E-4 45.000004 28 TTGATAT 20 7.023042E-4 45.000004 37 CTTCGCG 20 7.023042E-4 45.000004 28 TAGAGTC 20 7.023042E-4 45.000004 33 TTCTACG 20 7.023042E-4 45.000004 1 CCCCTAT 20 7.023042E-4 45.000004 28 CGTATTT 20 7.023042E-4 45.000004 34 ATTAGTG 20 7.023042E-4 45.000004 24 GATCGTG 20 7.023042E-4 45.000004 29 TTGTGTC 30 2.1588949E-6 45.000004 39 CAAGTAT 20 7.023042E-4 45.000004 42 ACCATTA 20 7.023042E-4 45.000004 10 CCCTATA 20 7.023042E-4 45.000004 29 TTAGCCA 20 7.023042E-4 45.000004 39 TGGACGT 20 7.023042E-4 45.000004 28 GACGTGA 20 7.023042E-4 45.000004 30 TAGGTAT 20 7.023042E-4 45.000004 19 TTAGAGT 20 7.023042E-4 45.000004 32 TATGGGC 30 2.1588949E-6 45.000004 4 GGCCGTA 30 2.1588949E-6 45.000004 42 >>END_MODULE