SRR3548409_1.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR3548409_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	361163
Sequences flagged as poor quality	0
Sequence length	51
%GC	47

Per base sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
AATGATACCTGTCTCTTATACACATCTGACGCCCCATGTTTCGTATGCCGT	9718	2.690751821199846	No Hit
AATGATACGGCTGTCTCTTATACACATCTGACGCCCCATGTTTCGTATGCC	9662	2.6752463569081	No Hit
AATCTGTCTCTTATACACATCTGACGCCCCATGTTTCGTATGCCGTCTTCT	8547	2.366521487527792	No Hit
CTGTCTCTTATACACATCTGACGCCCCATGTTTCGTATGCCGTCTTCTGCT	6133	1.6981252232371533	Illumina Paired End PCR Primer 2 (95% over 21bp)
AATGACTGTCTCTTATACACATCTGACGCCCCATGTTTCGTATGCCGTCTT	2221	0.6149577891422986	No Hit
CAGCAGGGAATGATACCTGTCTCTTATACACATCTGACGCCCCATGTTTCG	1879	0.520263703646276	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1759	0.48703770873539093	No Hit
AATGATCTGTCTCTTATACACATCTGACGCCCCATGTTTCGTATGCCGTCT	1722	0.4767930269712014	No Hit
AAACTGTCTCTTATACACATCTGACGCCCCATGTTTCGTATGCCGTCTTCT	1601	0.4432901487693922	No Hit
AACTGTCTCTTATACACATCTGACGCCCCATGTTTCGTATGCCGTCTTCTG	1450	0.4014807718398618	No Hit
AATGATACGGCGACTGTCTCTTATACACATCTGACGCCCCATGTTTCGTAT	1348	0.37323867616560946	No Hit
AAAAACTGTCTCTTATACACATCTGACGCCCCATGTTTCGTATGCCGTCTT	1308	0.36216334452864773	No Hit
AATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCCCCATGTTTC	1286	0.3560719121283188	No Hit
CCTGTCTCTTATACACATCTGACGCCCCATGTTTCGTATGCCGTCTTCTGC	1014	0.2807596569969792	TruSeq Adapter, Index 14 (95% over 21bp)
CAGCAGGGAATGATACGGCTGTCTCTTATACACATCTGACGCCCCATGTTT	798	0.220952866157386	No Hit
AAAAAACTGTCTCTTATACACATCTGACGCCCCATGTTTCGTATGCCGTCT	736	0.20378610212009535	No Hit
AATGCTGTCTCTTATACACATCTGACGCCCCATGTTTCGTATGCCGTCTTC	628	0.17388270670029876	No Hit
AGCCCAGGGAATGATACCTGTCTCTTATACACATCTGACGCCCCATGTTTC	537	0.1486863272262109	No Hit
GAAAAACTGTCTCTTATACACATCTGACGCCCCATGTTTCGTATGCCGTCT	491	0.13594969584370492	No Hit
AGCCTGTCTCTTATACACATCTGACGCCCCATGTTTCGTATGCCGTCTTCT	473	0.13096579660707217	No Hit
ACCTGTCTCTTATACACATCTGACGCCCCATGTTTCGTATGCCGTCTTCTG	472	0.1306889133161481	No Hit
AACCTGTCTCTTATACACATCTGACGCCCCATGTTTCGTATGCCGTCTTCT	469	0.12985826344337598	No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	437	0.12099799813380663	No Hit
CAGCAGGGAATCTGTCTCTTATACACATCTGACGCCCCATGTTTCGTATGC	418	0.1157372156062498	No Hit
CAGCAGCTGTCTCTTATACACATCTGACGCCCCATGTTTCGTATGCCGTCT	405	0.11213773282423725	No Hit
TACCTGTCTCTTATACACATCTGACGCCCCATGTTTCGTATGCCGTCTTCT	395	0.10936889991499682	No Hit
CACCTGTCTCTTATACACATCTGACGCCCCATGTTTCGTATGCCGTCTTCT	390	0.10798448346037662	No Hit
AATGATACGGCGCTGTCTCTTATACACATCTGACGCCCCATGTTTCGTATG	388	0.10743071687852852	No Hit
GCTGTCTCTTATACACATCTGACGCCCCATGTTTCGTATGCCGTCTTCTGC	385	0.1066000670057564	TruSeq Adapter, Index 14 (95% over 21bp)

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGTTCA	30	2.1613523E-6	45.000004	16
TCCGCCC	35	1.2088276E-7	45.000004	35
GATCGTC	30	2.1613523E-6	45.000004	9
ACGCATG	35	1.2088276E-7	45.000004	1
GCGAAAC	30	2.1613523E-6	45.000004	38
ATGTGAC	30	2.1613523E-6	45.000004	21
CGTTGAT	35	1.2088276E-7	45.000004	25
ATAGCGG	60	0.0	45.000004	2
CGACCGG	30	2.1613523E-6	45.000004	2
CCTCGTT	30	2.1613523E-6	45.000004	14
CGTTCAT	30	2.1613523E-6	45.000004	17
TGCATAG	30	2.1613523E-6	45.000004	1
CGGCTAG	30	2.1613523E-6	45.000004	1
TGGGACG	35	1.2088276E-7	45.000004	6
ACGAACG	30	2.1613523E-6	45.000004	1
TCGCCTT	25	3.8854756E-5	45.0	29
AACGTAG	20	7.026856E-4	45.0	1
CGAACAG	40	6.7921064E-9	45.0	1
CTCGTCC	20	7.026856E-4	45.0	37
CTCGTCA	25	3.8854756E-5	45.0	36

Summary

Basic Statistics

Per base sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content