##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3548409_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	361163
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.353538430016364	31.0	31.0	34.0	30.0	34.0
2	31.55891661105927	31.0	31.0	34.0	30.0	34.0
3	31.708879370256643	31.0	31.0	34.0	30.0	34.0
4	35.42440393949546	37.0	35.0	37.0	33.0	37.0
5	35.13029850787594	37.0	35.0	37.0	32.0	37.0
6	35.16140634561126	37.0	35.0	37.0	32.0	37.0
7	35.14557415903622	37.0	35.0	37.0	32.0	37.0
8	35.30968288556691	37.0	35.0	37.0	33.0	37.0
9	36.96741083665824	39.0	37.0	39.0	33.0	39.0
10	36.51695217948682	38.0	35.0	39.0	32.0	39.0
11	36.61827208213466	38.0	35.0	39.0	32.0	39.0
12	36.529893704504616	38.0	35.0	39.0	32.0	39.0
13	36.558130262513046	38.0	35.0	39.0	32.0	39.0
14	37.546994016552084	39.0	36.0	40.0	32.0	41.0
15	37.6602337448742	39.0	36.0	40.0	33.0	41.0
16	37.408472074935695	39.0	36.0	40.0	32.0	41.0
17	37.369893926011244	39.0	36.0	40.0	32.0	41.0
18	37.189244191680764	39.0	36.0	40.0	32.0	41.0
19	37.16837549804382	39.0	36.0	40.0	31.0	41.0
20	37.24415291710391	39.0	36.0	40.0	32.0	41.0
21	37.24100752291901	39.0	36.0	40.0	32.0	41.0
22	37.229937728947874	39.0	36.0	40.0	32.0	41.0
23	37.074074586820906	39.0	36.0	40.0	31.0	41.0
24	36.89534088486362	39.0	35.0	40.0	31.0	41.0
25	37.02966804462251	39.0	36.0	40.0	31.0	41.0
26	36.95123531480246	39.0	36.0	40.0	31.0	41.0
27	36.89372111761172	39.0	36.0	40.0	31.0	41.0
28	36.70395915417692	39.0	35.0	40.0	30.0	41.0
29	36.62641798855365	39.0	35.0	40.0	30.0	41.0
30	36.76272486384264	39.0	36.0	40.0	30.0	41.0
31	36.394112907468376	39.0	35.0	40.0	30.0	41.0
32	36.51670852219081	39.0	35.0	40.0	30.0	41.0
33	36.49396809750722	39.0	35.0	40.0	30.0	41.0
34	36.38002231679325	39.0	35.0	40.0	30.0	41.0
35	36.49085039165141	39.0	35.0	40.0	30.0	41.0
36	36.32907025359741	39.0	35.0	40.0	30.0	41.0
37	36.20902196515147	38.0	35.0	40.0	30.0	41.0
38	36.07744425647145	38.0	35.0	40.0	30.0	41.0
39	35.87449711072286	38.0	35.0	40.0	29.0	41.0
40	35.78999509916575	38.0	35.0	40.0	28.0	41.0
41	35.80227210428532	38.0	35.0	40.0	29.0	41.0
42	35.80063849286887	38.0	35.0	40.0	29.0	41.0
43	35.841955017540556	38.0	35.0	40.0	29.0	41.0
44	35.760922353618724	38.0	35.0	40.0	28.0	41.0
45	35.427942507953475	38.0	34.0	40.0	27.0	41.0
46	35.20302744190296	38.0	34.0	40.0	26.0	41.0
47	35.07478063921277	38.0	34.0	40.0	26.0	41.0
48	35.024997023504625	38.0	34.0	40.0	26.0	41.0
49	34.897146163920446	38.0	34.0	40.0	26.0	41.0
50	34.86174109750999	38.0	34.0	40.0	26.0	41.0
51	33.23880906958908	36.0	31.0	39.0	21.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	0.0
12	5.0
13	3.0
14	6.0
15	6.0
16	23.0
17	60.0
18	137.0
19	314.0
20	605.0
21	1051.0
22	1545.0
23	1815.0
24	2267.0
25	2481.0
26	3082.0
27	3684.0
28	4628.0
29	6249.0
30	8291.0
31	10821.0
32	14190.0
33	18605.0
34	24700.0
35	31483.0
36	37824.0
37	50655.0
38	72055.0
39	64566.0
40	11.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	16.036249560447775	41.20327940569771	16.87409839878393	25.886372635070593
2	18.497188250180667	41.95889390662942	18.54758100912884	20.996336834061076
3	19.60139881438575	27.996777078493647	29.334677140238618	23.067146966881992
4	25.38742894482547	28.024465407586046	17.475765789961873	29.112339857626612
5	14.676475718719804	40.14060133513123	19.136234885633357	26.04668806051561
6	21.63427593635007	31.589337778233094	25.93399656110953	20.842389724307306
7	69.50878135357166	13.093810827797975	10.499691275130619	6.897716543499749
8	71.99630083923326	4.418503556565872	7.196750497697716	16.38844510650316
9	68.02219496460047	9.957000024919497	14.385748263249557	7.6350567472304744
10	27.07586325288028	39.50404664929685	15.576623297513864	17.843466800309002
11	18.40553988088481	24.164989215395817	36.13216193242386	21.297308971295507
12	21.549826532618237	19.45160495399584	41.66899710103195	17.329571412353978
13	23.45063032481179	27.395663453897544	27.36437564202313	21.78933057926753
14	14.549940054767516	26.332708500040148	40.19403981027957	18.923311634912768
15	13.094641477670747	31.66631133310998	28.53891456212292	26.70013262709635
16	15.286172725334543	29.264625667634835	31.083748889005797	24.365452718024823
17	15.702882078175229	27.79105279333708	30.917895797742297	25.588169330745398
18	17.63635809869782	26.460905463737983	27.881870512760166	28.020865924804035
19	16.823151873253906	31.094270454060908	32.66032234752729	19.42225532515789
20	19.55349800505589	31.66908016601922	28.07291998349776	20.704501845427135
21	17.08176086697696	28.30217934838286	28.595398753471425	26.02066103116875
22	16.114607531779278	30.372712597912855	29.99836638858355	23.51431348172432
23	20.411282440338574	26.661922732948835	23.776245074938462	29.15054975177413
24	14.441124921434367	35.28296087915982	25.514241492068678	24.76167270733713
25	16.750054684449957	25.304363957548254	28.0377558055504	29.907825552451385
26	21.46177764610439	28.742977547533936	22.87692814601717	26.918316660344498
27	14.328156538737357	25.884711335325044	28.587922904616477	31.199209221321123
28	19.296550311078377	26.40275997264393	24.39590988002647	29.904779836251222
29	17.400453534830536	28.948701832690503	25.35503359978735	28.29581103269161
30	20.930992377403	23.286161650002906	22.127128194194864	33.65571777839922
31	19.979898273078913	32.24859689392324	21.96293640267691	25.808568430320932
32	18.031470554846425	23.299175164676335	26.849926487486258	31.81942779299098
33	23.692903204370324	24.327242823877306	23.601254835074467	28.378599136677902
34	13.885697039840736	22.83096551972378	31.122235666444237	32.16110177399125
35	17.850388882582102	21.512170405052565	29.417742127515833	31.219698584849496
36	18.17544986612693	24.889869671034962	31.07987252293286	25.85480793990525
37	17.213003546874955	24.72955424558994	29.208972126159104	28.848470081376
38	24.12317983846629	26.265979626927454	29.733665962460165	19.877174572146096
39	19.209885841019155	20.811655679014738	36.17590949238986	23.802548987576248
40	24.175787663741858	23.1593491027597	28.909384405379292	23.75547882811916
41	17.866724996746623	18.862951077491328	38.214878046754514	25.055445879007536
42	22.673972693769848	21.52352261998045	28.705875186550116	27.09662949969958
43	19.20850142456453	20.51677497418063	30.166711429465366	30.10801217178947
44	19.54712968936464	18.757181660358345	28.57740133956136	33.11828731071566
45	26.831652190285272	22.90683154143697	27.065064804534238	23.196451463743518
46	16.92975194025966	19.93227434703998	38.7008082223262	24.437165490374152
47	22.21573084729056	23.186760548561175	26.694871844568794	27.90263675957947
48	18.559486990638575	18.534567494455413	39.22079504268156	23.685150472224453
49	22.107746363830184	17.250659674440627	33.454146742606525	27.187447219122667
50	20.18091554228977	18.502172149417298	27.639043866619783	33.67786844167315
51	17.9378840025141	16.50778180489142	38.31400226490532	27.24033192768916
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	2096.0
1	1638.5
2	1181.0
3	827.0
4	473.0
5	419.5
6	366.0
7	367.5
8	369.0
9	398.0
10	427.0
11	496.5
12	566.0
13	582.0
14	598.0
15	631.5
16	665.0
17	747.5
18	830.0
19	939.0
20	1048.0
21	1013.5
22	979.0
23	1345.5
24	1712.0
25	1668.5
26	1875.5
27	2126.0
28	2796.5
29	3467.0
30	3812.5
31	4158.0
32	4868.5
33	5579.0
34	6403.5
35	7228.0
36	7744.0
37	8260.0
38	9294.0
39	10328.0
40	11151.0
41	11974.0
42	15181.0
43	18388.0
44	33325.0
45	48262.0
46	48726.5
47	49191.0
48	49962.5
49	50734.0
50	43964.5
51	37195.0
52	31758.5
53	26322.0
54	22684.0
55	19046.0
56	16295.0
57	13544.0
58	11800.0
59	10056.0
60	8854.0
61	7652.0
62	6592.5
63	5533.0
64	4458.5
65	3384.0
66	2706.0
67	2028.0
68	1744.5
69	1461.0
70	1051.0
71	641.0
72	617.5
73	594.0
74	572.5
75	365.0
76	179.0
77	160.5
78	142.0
79	125.0
80	108.0
81	80.5
82	53.0
83	34.0
84	15.0
85	10.5
86	6.0
87	6.0
88	6.0
89	11.0
90	16.0
91	8.5
92	1.0
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	361163.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	28.97482831408275
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.69531090215438	21.063341526287914
2	11.823137878653114	6.8514678033540495
3	4.476519842929062	3.8911918168036275
4	2.194480843942451	2.543388227671038
5	1.3349485354637287	1.9339952311598876
6	0.9450544563843543	1.6429674372717282
7	0.6828628498924164	1.3850083686388617
8	0.5343266374814906	1.2385618067733857
9	0.44660122628560106	1.16461744708357
>10	4.1968550272954275	26.84830107055206
>50	0.5452920897861915	10.281965474696197
>100	0.10666591153038847	6.035098087712513
>500	0.003987510711416392	0.7795888646853678
>1k	0.009968776778540979	4.502493969142464
>5k	0.003987510711416392	9.838012868167331
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AATGATACCTGTCTCTTATACACATCTGACGCCCCATGTTTCGTATGCCGT	9718	2.690751821199846	No Hit
AATGATACGGCTGTCTCTTATACACATCTGACGCCCCATGTTTCGTATGCC	9662	2.6752463569081	No Hit
AATCTGTCTCTTATACACATCTGACGCCCCATGTTTCGTATGCCGTCTTCT	8547	2.366521487527792	No Hit
CTGTCTCTTATACACATCTGACGCCCCATGTTTCGTATGCCGTCTTCTGCT	6133	1.6981252232371533	Illumina Paired End PCR Primer 2 (95% over 21bp)
AATGACTGTCTCTTATACACATCTGACGCCCCATGTTTCGTATGCCGTCTT	2221	0.6149577891422986	No Hit
CAGCAGGGAATGATACCTGTCTCTTATACACATCTGACGCCCCATGTTTCG	1879	0.520263703646276	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1759	0.48703770873539093	No Hit
AATGATCTGTCTCTTATACACATCTGACGCCCCATGTTTCGTATGCCGTCT	1722	0.4767930269712014	No Hit
AAACTGTCTCTTATACACATCTGACGCCCCATGTTTCGTATGCCGTCTTCT	1601	0.4432901487693922	No Hit
AACTGTCTCTTATACACATCTGACGCCCCATGTTTCGTATGCCGTCTTCTG	1450	0.4014807718398618	No Hit
AATGATACGGCGACTGTCTCTTATACACATCTGACGCCCCATGTTTCGTAT	1348	0.37323867616560946	No Hit
AAAAACTGTCTCTTATACACATCTGACGCCCCATGTTTCGTATGCCGTCTT	1308	0.36216334452864773	No Hit
AATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCCCCATGTTTC	1286	0.3560719121283188	No Hit
CCTGTCTCTTATACACATCTGACGCCCCATGTTTCGTATGCCGTCTTCTGC	1014	0.2807596569969792	TruSeq Adapter, Index 14 (95% over 21bp)
CAGCAGGGAATGATACGGCTGTCTCTTATACACATCTGACGCCCCATGTTT	798	0.220952866157386	No Hit
AAAAAACTGTCTCTTATACACATCTGACGCCCCATGTTTCGTATGCCGTCT	736	0.20378610212009535	No Hit
AATGCTGTCTCTTATACACATCTGACGCCCCATGTTTCGTATGCCGTCTTC	628	0.17388270670029876	No Hit
AGCCCAGGGAATGATACCTGTCTCTTATACACATCTGACGCCCCATGTTTC	537	0.1486863272262109	No Hit
GAAAAACTGTCTCTTATACACATCTGACGCCCCATGTTTCGTATGCCGTCT	491	0.13594969584370492	No Hit
AGCCTGTCTCTTATACACATCTGACGCCCCATGTTTCGTATGCCGTCTTCT	473	0.13096579660707217	No Hit
ACCTGTCTCTTATACACATCTGACGCCCCATGTTTCGTATGCCGTCTTCTG	472	0.1306889133161481	No Hit
AACCTGTCTCTTATACACATCTGACGCCCCATGTTTCGTATGCCGTCTTCT	469	0.12985826344337598	No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	437	0.12099799813380663	No Hit
CAGCAGGGAATCTGTCTCTTATACACATCTGACGCCCCATGTTTCGTATGC	418	0.1157372156062498	No Hit
CAGCAGCTGTCTCTTATACACATCTGACGCCCCATGTTTCGTATGCCGTCT	405	0.11213773282423725	No Hit
TACCTGTCTCTTATACACATCTGACGCCCCATGTTTCGTATGCCGTCTTCT	395	0.10936889991499682	No Hit
CACCTGTCTCTTATACACATCTGACGCCCCATGTTTCGTATGCCGTCTTCT	390	0.10798448346037662	No Hit
AATGATACGGCGCTGTCTCTTATACACATCTGACGCCCCATGTTTCGTATG	388	0.10743071687852852	No Hit
GCTGTCTCTTATACACATCTGACGCCCCATGTTTCGTATGCCGTCTTCTGC	385	0.1066000670057564	TruSeq Adapter, Index 14 (95% over 21bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	1.9780542303613604	0.0
2	0.0	0.0	0.0	2.62319229821438	0.0
3	0.0	0.0	0.0	3.6678729548707922	0.0
4	0.0	0.0	0.0	9.167328879204126	0.0
5	0.0	0.0	0.0	9.830464360967209	0.0
6	0.0	0.0	0.0	12.783424658672123	0.0
7	0.0	0.0	0.0	15.124472883434903	0.0
8	0.0	0.0	0.0	16.357987944501513	0.0
9	0.0	0.0	0.0	20.903304048310595	0.0
10	0.0	0.0	0.0	23.386947167899258	0.0
11	0.0	0.0	0.0	28.284458817763724	0.0
12	0.0	0.0	0.0	29.708746466277	0.0
13	0.0	0.0	0.0	30.381849746513346	0.0
14	0.0	0.0	0.0	31.255139646087777	0.0
15	0.0	0.0	0.0	31.85403820435648	0.0
16	0.0	0.0	0.0	32.85857078382891	0.0
17	0.0	0.0	0.0	34.52347001215518	0.0
18	0.0	0.0	0.0	36.84153692377126	0.0
19	0.0	0.0	0.0	37.7713110146942	0.0
20	0.0	0.0	0.0	38.659275728687604	0.0
21	0.0	0.0	0.0	39.38969385014523	0.0
22	0.0	0.0	0.0	40.120388854893775	0.0
23	0.0	0.0	0.0	40.80982824929464	0.0
24	0.0	0.0	0.0	41.28108361044736	0.0
25	0.0	0.0	0.0	41.69751607999712	0.0
26	0.0	0.0	0.0	42.19175275429654	0.0
27	0.0	0.0	0.0	42.773761431818876	0.0
28	0.0	0.0	0.0	43.24557055955344	0.0
29	0.0	0.0	0.0	43.71931787032448	0.0
30	0.0	0.0	0.0	44.455273657600586	0.0
31	0.0	0.0	0.0	44.85121676362196	0.0
32	0.0	0.0	0.0	45.23082375547883	0.0
33	0.0	0.0	0.0	45.622336728845426	0.0
34	0.0	0.0	0.0	46.01994113461235	0.0
35	0.0	0.0	0.0	46.42945152188901	0.0
36	0.0	0.0	0.0	46.81238111323696	0.0
37	0.0	0.0	0.0	47.19641823774861	0.0
38	0.0	0.0	0.0	47.57297951340531	0.0
39	0.0	0.0	0.0	47.95729352120787	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGTTCA	30	2.1613523E-6	45.000004	16
TCCGCCC	35	1.2088276E-7	45.000004	35
GATCGTC	30	2.1613523E-6	45.000004	9
ACGCATG	35	1.2088276E-7	45.000004	1
GCGAAAC	30	2.1613523E-6	45.000004	38
ATGTGAC	30	2.1613523E-6	45.000004	21
CGTTGAT	35	1.2088276E-7	45.000004	25
ATAGCGG	60	0.0	45.000004	2
CGACCGG	30	2.1613523E-6	45.000004	2
CCTCGTT	30	2.1613523E-6	45.000004	14
CGTTCAT	30	2.1613523E-6	45.000004	17
TGCATAG	30	2.1613523E-6	45.000004	1
CGGCTAG	30	2.1613523E-6	45.000004	1
TGGGACG	35	1.2088276E-7	45.000004	6
ACGAACG	30	2.1613523E-6	45.000004	1
TCGCCTT	25	3.8854756E-5	45.0	29
AACGTAG	20	7.026856E-4	45.0	1
CGAACAG	40	6.7921064E-9	45.0	1
CTCGTCC	20	7.026856E-4	45.0	37
CTCGTCA	25	3.8854756E-5	45.0	36
>>END_MODULE