FastQCFastQC Report
Sat 18 Jun 2016
SRR3548406_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3548406_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences386143
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTCTCTTATACACATCTGACGCTAGTCACCTCGTATGCCGTCTTCTGCT25210.6528669430754928TruSeq Adapter, Index 7 (95% over 24bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA13890.35971129866396645No Hit
TGCTAGGGGAATGATACGGCTGTCTCTTATACACATCTGACGCTAGTCACC8770.22711793299373548No Hit
TGCTAGGGGAATGATACCTGTCTCTTATACACATCTGACGCTAGTCACCTC8170.2115796479542553No Hit
AATCTGTCTCTTATACACATCTGACGCTAGTCACCTCGTATGCCGTCTTCT7780.2014797626785932TruSeq Adapter, Index 7 (95% over 21bp)
AAACTGTCTCTTATACACATCTGACGCTAGTCACCTCGTATGCCGTCTTCT7700.19940799133999582No Hit
AATGATACCTGTCTCTTATACACATCTGACGCTAGTCACCTCGTATGCCGT6930.17946719220599622No Hit
TGCTAGGGCTGTCTCTTATACACATCTGACGCTAGTCACCTCGTATGCCGT6700.17351084960752883No Hit
AATGATACGGCTGTCTCTTATACACATCTGACGCTAGTCACCTCGTATGCC6640.1719570211035808No Hit
AAAAACTGTCTCTTATACACATCTGACGCTAGTCACCTCGTATGCCGTCTT6510.1685903926783601No Hit
TGCAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGG5470.14165736527659442No Hit
AGCCTGTCTCTTATACACATCTGACGCTAGTCACCTCGTATGCCGTCTTCT4510.1167961092134261RNA PCR Primer, Index 7 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCTAGTCACCTCGTATGCCGTCTTCTGC4480.1160191949614521RNA PCR Primer, Index 7 (95% over 23bp)
AGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT4440.11498330929215342No Hit
TGCTAGGGGAATGATCTGTCTCTTATACACATCTGACGCTAGTCACCTCGT3990.10332959551254328No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATACGA253.885977E-545.00000432
CGAACAG650.045.0000041
CGCGCCA253.885977E-545.00000422
ATAGATC253.885977E-545.00000411
CGACACG253.885977E-545.0000041
TGCATAA253.885977E-545.00000422
GCCGATG502.1827873E-1145.0000049
ATATCCG253.885977E-545.0000041
ATTCACG253.885977E-545.0000041
AGACCGA253.885977E-545.00000445
AGACCAC253.885977E-545.00000445
GTCAAGC253.885977E-545.00000416
CTACCGG502.1827873E-1145.0000042
TTAGTCT253.885977E-545.00000414
GAATTCG253.885977E-545.00000412
TATCGAC253.885977E-545.00000434
TATCCGG253.885977E-545.0000042
GTCATAC207.0274604E-445.09
AGTCCAA207.0274604E-445.020
TCGCACG207.0274604E-445.01