FastQCFastQC Report
Sat 18 Jun 2016
SRR3548401_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3548401_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences500879
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTCTCTTATACACATCTGACGCAGTGCCATTCGTATGCCGTCTTCTGCT27900.5570207575083005No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA18930.3779355892341264No Hit
AAACTGTCTCTTATACACATCTGACGCAGTGCCATTCGTATGCCGTCTTCT10780.21522164035625369No Hit
AAAAACTGTCTCTTATACACATCTGACGCAGTGCCATTCGTATGCCGTCTT8960.17888551925714594No Hit
TGCAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGG8890.17748797613794948No Hit
AATGATACGGCTGTCTCTTATACACATCTGACGCAGTGCCATTCGTATGCC7730.154328690162694No Hit
AATGATACCTGTCTCTTATACACATCTGACGCAGTGCCATTCGTATGCCGT7470.14913781571996432No Hit
AATCTGTCTCTTATACACATCTGACGCAGTGCCATTCGTATGCCGTCTTCT7400.14774027260076786No Hit
CCTGTCTCTTATACACATCTGACGCAGTGCCATTCGTATGCCGTCTTCTGC6890.13755817273233656No Hit
TCTCACGGGGAGTTGGTGTAAACATGCCATGTAATAAATGATTTCCAGTTA5230.10441643590567783No Hit
AGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT5020.10022380654808845No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATGCG650.045.0000041
ACTATGC302.1630403E-645.0000044
CCTTAGT302.1630403E-645.00000417
AATCCGA302.1630403E-645.00000432
CCCTCGT302.1630403E-645.00000410
CCAATCG302.1630403E-645.00000439
TATGCAT302.1630403E-645.00000411
ATCCGAA302.1630403E-645.00000433
CCCAATC302.1630403E-645.00000438
AATCTAG302.1630403E-645.0000041
ATGCGAG302.1630403E-645.0000041
CAATACG302.1630403E-645.0000041
GATAGCG453.8380676E-1045.0000041
TCACGCG551.8189894E-1245.01
ACAACGC207.02947E-445.014
CTAGATC253.887644E-545.023
TTGATAC207.02947E-445.034
TTTAGCG253.887644E-545.01
AACGTAG207.02947E-445.01
ATGATTA253.887644E-545.032