SRR3548400_1.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR3548400_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	259219
Sequences flagged as poor quality	0
Sequence length	51
%GC	49

Per base sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
CTGTCTCTTATACACATCTGACGCAATGGGGTTCGTATGCCGTCTTCTGCT	2539	0.9794806707841632	Illumina Single End Adapter 1 (95% over 21bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2003	0.7727057044429614	No Hit
AATGATACCTGTCTCTTATACACATCTGACGCAATGGGGTTCGTATGCCGT	1435	0.553585963991837	No Hit
AATGATACGGCTGTCTCTTATACACATCTGACGCAATGGGGTTCGTATGCC	1401	0.5404696415000443	No Hit
AATCTGTCTCTTATACACATCTGACGCAATGGGGTTCGTATGCCGTCTTCT	1354	0.5223382545260956	No Hit
AAACTGTCTCTTATACACATCTGACGCAATGGGGTTCGTATGCCGTCTTCT	1293	0.4988060288790559	No Hit
AAAAACTGTCTCTTATACACATCTGACGCAATGGGGTTCGTATGCCGTCTT	1127	0.434767513183833	No Hit
AAAAAACTGTCTCTTATACACATCTGACGCAATGGGGTTCGTATGCCGTCT	588	0.2268352242698259	No Hit
CCTGTCTCTTATACACATCTGACGCAATGGGGTTCGTATGCCGTCTTCTGC	528	0.20368877281372122	No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	456	0.1759130310663956	No Hit
AATGACTGTCTCTTATACACATCTGACGCAATGGGGTTCGTATGCCGTCTT	389	0.15006616027374536	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	370	0.14273645064597887	No Hit
GAAAAACTGTCTCTTATACACATCTGACGCAATGGGGTTCGTATGCCGTCT	366	0.14119335388223858	No Hit
AACTGTCTCTTATACACATCTGACGCAATGGGGTTCGTATGCCGTCTTCTG	355	0.13694983778195272	No Hit
AGATTAGGGACAGAAATATGACAACAAAAATGTTTGAAAAGAATGATACTA	328	0.12653393462670562	No Hit
ACCTGTCTCTTATACACATCTGACGCAATGGGGTTCGTATGCCGTCTTCTG	324	0.12499083786296529	No Hit
TGCAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGG	308	0.11881845080800404	No Hit
GAAAACTGTCTCTTATACACATCTGACGCAATGGGGTTCGTATGCCGTCTT	280	0.10801677346182187	No Hit
GCTGTCTCTTATACACATCTGACGCAATGGGGTTCGTATGCCGTCTTCTGC	278	0.1072452250799517	No Hit
AATGATCTGTCTCTTATACACATCTGACGCAATGGGGTTCGTATGCCGTCT	274	0.1057021283162114	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TACCAGG	75	0.0	45.000004	2
CGGGACA	95	0.0	45.000004	6
TCACGCC	40	6.7793735E-9	45.0	43
ATTTAGT	20	7.023168E-4	45.0	15
CGGGTTA	20	7.023168E-4	45.0	6
AACGTGA	20	7.023168E-4	45.0	22
AGGTAAT	35	1.2070632E-7	45.0	6
GGTACTA	25	3.8824204E-5	45.0	31
CTCACAC	20	7.023168E-4	45.0	38
CATATTA	20	7.023168E-4	45.0	12
AATCATG	20	7.023168E-4	45.0	1
GGTAATC	20	7.023168E-4	45.0	8
AGATATC	20	7.023168E-4	45.0	31
CGAAATG	20	7.023168E-4	45.0	1
CCGATTG	25	3.8824204E-5	45.0	1
TACCAAA	20	7.023168E-4	45.0	35
CGACGAC	20	7.023168E-4	45.0	28
CCTAGCG	25	3.8824204E-5	45.0	1
TAGACAA	25	3.8824204E-5	45.0	39
ACACCAT	20	7.023168E-4	45.0	11

Summary

Basic Statistics

Per base sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content