##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3548400_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	259219
Sequences flagged as poor quality	0
Sequence length	51
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.539559214409437	31.0	31.0	34.0	30.0	34.0
2	31.724904424444198	31.0	31.0	34.0	30.0	34.0
3	31.927123397590453	33.0	31.0	34.0	30.0	34.0
4	35.63506918860114	37.0	35.0	37.0	33.0	37.0
5	35.37950921807429	37.0	35.0	37.0	33.0	37.0
6	34.75744833519148	37.0	35.0	37.0	32.0	37.0
7	35.1285553913872	37.0	35.0	37.0	32.0	37.0
8	35.50370536110393	37.0	35.0	37.0	33.0	37.0
9	37.13625544423827	39.0	37.0	39.0	34.0	39.0
10	36.7453273101123	39.0	37.0	39.0	32.0	39.0
11	36.775016491846664	39.0	37.0	39.0	32.0	39.0
12	36.621354916113404	39.0	35.0	39.0	32.0	39.0
13	36.56637437842134	39.0	35.0	39.0	32.0	39.0
14	37.62453369544671	39.0	36.0	41.0	33.0	41.0
15	37.65941925553297	39.0	36.0	41.0	33.0	41.0
16	37.57075291548845	39.0	36.0	41.0	32.0	41.0
17	37.552073729163375	39.0	36.0	41.0	32.0	41.0
18	37.458330600766146	39.0	36.0	41.0	32.0	41.0
19	37.38851704543263	39.0	35.0	41.0	32.0	41.0
20	37.314201505290896	39.0	35.0	40.0	32.0	41.0
21	37.28480551194164	39.0	35.0	40.0	32.0	41.0
22	37.279497259074375	39.0	35.0	40.0	32.0	41.0
23	37.185121461004016	39.0	35.0	40.0	32.0	41.0
24	37.035738121048226	39.0	35.0	40.0	31.0	41.0
25	37.05138512223255	39.0	35.0	40.0	31.0	41.0
26	36.9569707467431	39.0	35.0	40.0	31.0	41.0
27	36.89878442552436	39.0	35.0	40.0	31.0	41.0
28	36.745574205594494	39.0	35.0	40.0	31.0	41.0
29	36.46427923879037	39.0	35.0	40.0	30.0	41.0
30	36.53865650280265	39.0	35.0	40.0	31.0	41.0
31	36.42568638872922	38.0	35.0	40.0	30.0	41.0
32	36.33680015739587	38.0	35.0	40.0	30.0	41.0
33	36.05932821282391	38.0	35.0	40.0	30.0	41.0
34	35.945362801337865	38.0	35.0	40.0	29.0	41.0
35	35.719198824160266	38.0	35.0	40.0	28.0	41.0
36	35.85245680293497	38.0	35.0	40.0	29.0	41.0
37	35.75970511420845	38.0	35.0	40.0	29.0	41.0
38	35.618546480003396	38.0	35.0	40.0	29.0	41.0
39	35.601020758509215	38.0	35.0	40.0	28.0	41.0
40	35.460853563974865	38.0	34.0	40.0	28.0	41.0
41	35.31293616594463	38.0	34.0	40.0	27.0	41.0
42	35.17171966561093	38.0	34.0	40.0	27.0	41.0
43	35.16575173887716	38.0	34.0	40.0	27.0	41.0
44	35.17862502362867	38.0	34.0	40.0	27.0	41.0
45	34.96651480022683	37.0	34.0	40.0	26.0	41.0
46	34.78066422600195	37.0	34.0	40.0	26.0	41.0
47	34.67258958641149	37.0	33.0	40.0	25.0	41.0
48	34.581809203800645	37.0	33.0	40.0	25.0	41.0
49	34.373074504569495	37.0	33.0	40.0	24.0	41.0
50	34.3547579459839	37.0	33.0	40.0	24.0	41.0
51	33.11281966213896	35.0	31.0	39.0	21.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	2.0
12	3.0
13	3.0
14	8.0
15	15.0
16	30.0
17	85.0
18	173.0
19	318.0
20	561.0
21	907.0
22	1249.0
23	1530.0
24	2016.0
25	2247.0
26	2511.0
27	2845.0
28	3425.0
29	4387.0
30	5540.0
31	7330.0
32	9403.0
33	12944.0
34	19704.0
35	24012.0
36	26991.0
37	36586.0
38	49482.0
39	44910.0
40	1.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	20.86112514900528	34.99473418229373	19.082320354603635	25.061820314097343
2	23.129863165894477	34.349333960859354	18.696160389477622	23.824642483768553
3	20.009721509611563	32.77074597155301	21.306694339535294	25.91283817930013
4	19.711518060018747	33.12835864654983	19.338088643193593	27.822034650237832
5	17.268024334635964	35.74622230623527	19.23315806325925	27.752595295869515
6	19.14867351544447	38.50720819075762	19.843452833318544	22.500665460479365
7	76.9391904142829	9.08883993843044	8.136749235202668	5.835220412083991
8	78.74615672462281	6.7633931154737885	6.118378668230338	8.372071491673063
9	72.7952040552583	9.317989807845874	11.942025854586277	5.944780282309553
10	33.97976228594354	31.164382240499343	14.211921194048276	20.643934279508834
11	24.728897187320374	29.651375863651968	26.24267511254962	19.377051836478035
12	24.990452088774358	23.14529413353188	32.25187968474533	19.612374092948436
13	27.010365752510424	24.624352381576966	26.822493721525042	21.542788144387565
14	19.348504546348842	27.87064219829565	31.07796882172989	21.70288443362562
15	16.908097014493535	30.219235472708405	30.095016183227308	22.77765132957075
16	19.664839382915606	28.562335322642245	27.64419274821676	24.128632546225393
17	18.36670923041907	27.594427877586135	29.176102060419957	24.862760831574846
18	21.47489188678299	26.15857633892577	26.605302852028593	25.761228922262642
19	22.936976070426937	32.46984210262365	25.253164312801147	19.34001751414827
20	23.36557119655581	29.24939915669762	24.692248639181543	22.69278100756503
21	21.39966591955065	30.09733082837292	24.70883692939175	23.794166322684678
22	19.035255903309555	29.473148187439964	25.650511729464277	25.841084179786204
23	22.45630142852183	27.736778554041173	23.544184646958747	26.262735370478246
24	18.42766155258681	30.97535288694116	23.36865739008329	27.228328170388743
25	22.085186656842286	28.507555387529464	23.4666440345808	25.940613921047458
26	22.075156527877972	29.828446217291173	23.8207847418592	24.275612512971655
27	18.700403905577907	27.25610391213607	25.646653987554924	28.396838194731096
28	21.930105432086382	30.100802796091337	25.5968891169243	22.372202654897983
29	23.451213066943396	32.542753424710384	21.562462628125253	22.44357088022097
30	24.28024180326288	28.31351096948912	24.27214054525324	23.134106681994762
31	26.411644208179187	31.1242617246421	18.949614032921968	23.51448003425675
32	27.03196910720279	26.410486885606378	23.293045648660012	23.264498358530815
33	26.775429270230962	26.895790817802705	23.44774109922498	22.88103881274135
34	25.84687079265023	27.843252230739257	24.577287930282885	21.73258904632762
35	24.998939120974928	27.80930410193697	26.390426627677755	20.801330149410344
36	27.19978088025955	27.72019026383097	26.332560499037495	18.747468356871988
37	28.237513453874907	26.72566439960034	23.49866329242841	21.53815885409634
38	30.33149576227051	25.838769534640594	24.8226403157176	19.007094387371296
39	30.488120083790154	21.903872787102795	25.105798571863946	22.502208557243105
40	30.097716602563857	23.711224871633636	24.083882740076923	22.107175785725584
41	23.375987099711054	22.28386036517385	30.988083435242014	23.35206909987308
42	25.389342602201225	25.607690794270482	25.857672469996412	23.14529413353188
43	20.866140213487437	24.541410930525924	28.163444809215378	26.429004046771265
44	25.34112082833434	20.577966892858935	27.79503045687237	26.28588182193435
45	29.313823446583775	21.636145498593855	26.28433872517061	22.765692329651763
46	22.597494782404066	23.9681504827964	29.738175056612366	23.69617967818717
47	25.799420567165214	23.290731003514402	26.161662532453256	24.748185896867128
48	24.365112125268595	21.660449272622763	31.229192304576443	22.7452462975322
49	24.47274312453948	20.194507347069464	29.785239507906443	25.54751002048461
50	22.194746527067846	19.634363221831734	28.27339045363187	29.89749979746855
51	23.293045648660012	20.101921541245048	31.4409823353998	25.164050474695145
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	2370.0
1	1816.5
2	1263.0
3	884.0
4	505.0
5	495.0
6	485.0
7	520.5
8	556.0
9	628.0
10	700.0
11	750.0
12	800.0
13	828.0
14	856.0
15	898.0
16	940.0
17	949.5
18	959.0
19	899.0
20	839.0
21	852.5
22	866.0
23	933.5
24	1001.0
25	1024.0
26	1296.0
27	1545.0
28	2086.5
29	2628.0
30	2688.5
31	2749.0
32	3228.5
33	3708.0
34	3862.0
35	4016.0
36	4273.5
37	4531.0
38	5226.5
39	5922.0
40	6410.0
41	6898.0
42	9200.5
43	11503.0
44	15654.5
45	19806.0
46	22153.0
47	24500.0
48	26344.0
49	28188.0
50	26670.5
51	25153.0
52	22672.5
53	20192.0
54	18122.5
55	16053.0
56	14846.5
57	13640.0
58	12971.0
59	12302.0
60	11305.0
61	10308.0
62	9111.5
63	7915.0
64	7424.0
65	6933.0
66	5964.5
67	4996.0
68	4488.0
69	3980.0
70	3370.0
71	2760.0
72	2321.5
73	1883.0
74	1772.0
75	1244.0
76	827.0
77	716.0
78	605.0
79	449.5
80	294.0
81	277.0
82	260.0
83	246.0
84	232.0
85	131.5
86	31.0
87	19.0
88	7.0
89	5.0
90	3.0
91	2.5
92	2.0
93	1.5
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	259219.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	35.31492676076985
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.67278765170467	27.430088072247788
2	9.747331854975258	6.884526211427403
3	3.613602350807817	3.828423070839715
4	1.8078935582185423	2.553825143990217
5	1.0312093770140809	1.8208541812135683
6	0.6991250013654785	1.4813728931906998
7	0.47409414155096513	1.171981992060767
8	0.38342636793637963	1.0832539281456992
9	0.3386386725364037	1.0763099927088677
>10	3.5141955146761634	29.033365609773977
>50	0.6368591809313656	14.439913740890905
>100	0.0710048829511814	4.463407389118853
>500	0.0021847656292671206	0.4305239970835471
>1k	0.007646679702434921	4.30215377730799
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTGTCTCTTATACACATCTGACGCAATGGGGTTCGTATGCCGTCTTCTGCT	2539	0.9794806707841632	Illumina Single End Adapter 1 (95% over 21bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2003	0.7727057044429614	No Hit
AATGATACCTGTCTCTTATACACATCTGACGCAATGGGGTTCGTATGCCGT	1435	0.553585963991837	No Hit
AATGATACGGCTGTCTCTTATACACATCTGACGCAATGGGGTTCGTATGCC	1401	0.5404696415000443	No Hit
AATCTGTCTCTTATACACATCTGACGCAATGGGGTTCGTATGCCGTCTTCT	1354	0.5223382545260956	No Hit
AAACTGTCTCTTATACACATCTGACGCAATGGGGTTCGTATGCCGTCTTCT	1293	0.4988060288790559	No Hit
AAAAACTGTCTCTTATACACATCTGACGCAATGGGGTTCGTATGCCGTCTT	1127	0.434767513183833	No Hit
AAAAAACTGTCTCTTATACACATCTGACGCAATGGGGTTCGTATGCCGTCT	588	0.2268352242698259	No Hit
CCTGTCTCTTATACACATCTGACGCAATGGGGTTCGTATGCCGTCTTCTGC	528	0.20368877281372122	No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	456	0.1759130310663956	No Hit
AATGACTGTCTCTTATACACATCTGACGCAATGGGGTTCGTATGCCGTCTT	389	0.15006616027374536	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	370	0.14273645064597887	No Hit
GAAAAACTGTCTCTTATACACATCTGACGCAATGGGGTTCGTATGCCGTCT	366	0.14119335388223858	No Hit
AACTGTCTCTTATACACATCTGACGCAATGGGGTTCGTATGCCGTCTTCTG	355	0.13694983778195272	No Hit
AGATTAGGGACAGAAATATGACAACAAAAATGTTTGAAAAGAATGATACTA	328	0.12653393462670562	No Hit
ACCTGTCTCTTATACACATCTGACGCAATGGGGTTCGTATGCCGTCTTCTG	324	0.12499083786296529	No Hit
TGCAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGG	308	0.11881845080800404	No Hit
GAAAACTGTCTCTTATACACATCTGACGCAATGGGGTTCGTATGCCGTCTT	280	0.10801677346182187	No Hit
GCTGTCTCTTATACACATCTGACGCAATGGGGTTCGTATGCCGTCTTCTGC	278	0.1072452250799517	No Hit
AATGATCTGTCTCTTATACACATCTGACGCAATGGGGTTCGTATGCCGTCT	274	0.1057021283162114	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	1.0782388636635432	0.0
2	0.0	0.0	0.0	1.5874607956978461	0.0
3	0.0	0.0	0.0	2.208943017294257	0.0
4	0.0	0.0	0.0	5.179790061685293	0.0
5	0.0	0.0	0.0	5.660078929399465	0.0
6	0.0	0.0	0.0	8.120546719183393	0.0
7	0.0	0.0	0.0	9.923269513423014	0.0
8	0.0	0.0	0.0	11.359121052083374	0.0
9	0.0	0.0	0.0	13.576551101578202	0.0
10	0.0	0.0	0.0	16.48143075931934	0.0
11	0.0	0.0	0.0	19.084249225558313	0.0
12	0.0	0.0	0.0	20.27011908849274	0.0
13	0.0	0.0	0.0	20.82524814924832	0.0
14	0.0	0.0	0.0	21.38307762934044	0.0
15	0.0	0.0	0.0	21.817845142524273	0.0
16	0.0	0.0	0.0	22.687765943082876	0.0
17	0.0	0.0	0.0	23.683449129886313	0.0
18	0.0	0.0	0.0	25.461096601715152	0.0
19	0.0	0.0	0.0	26.015454114088858	0.0
20	0.0	0.0	0.0	26.770414205748807	0.0
21	0.0	0.0	0.0	27.342903105096465	0.0
22	0.0	0.0	0.0	27.944710842955185	0.0
23	0.0	0.0	0.0	28.500997226283566	0.0
24	0.0	0.0	0.0	28.914932933156905	0.0
25	0.0	0.0	0.0	29.247856059933877	0.0
26	0.0	0.0	0.0	29.57614989641963	0.0
27	0.0	0.0	0.0	30.02904879657741	0.0
28	0.0	0.0	0.0	30.408264826266592	0.0
29	0.0	0.0	0.0	30.78400888823736	0.0
30	0.0	0.0	0.0	31.399704496969743	0.0
31	0.0	0.0	0.0	31.760403365494042	0.0
32	0.0	0.0	0.0	32.116858717918056	0.0
33	0.0	0.0	0.0	32.49337432827069	0.0
34	0.0	0.0	0.0	32.83902800334852	0.0
35	0.0	0.0	0.0	33.19548335577253	0.0
36	0.0	0.0	0.0	33.49368680536535	0.0
37	0.0	0.0	0.0	33.78803251304881	0.0
38	0.0	0.0	0.0	34.11516902696176	0.0
39	0.0	0.0	0.0	34.47393902453138	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TACCAGG	75	0.0	45.000004	2
CGGGACA	95	0.0	45.000004	6
TCACGCC	40	6.7793735E-9	45.0	43
ATTTAGT	20	7.023168E-4	45.0	15
CGGGTTA	20	7.023168E-4	45.0	6
AACGTGA	20	7.023168E-4	45.0	22
AGGTAAT	35	1.2070632E-7	45.0	6
GGTACTA	25	3.8824204E-5	45.0	31
CTCACAC	20	7.023168E-4	45.0	38
CATATTA	20	7.023168E-4	45.0	12
AATCATG	20	7.023168E-4	45.0	1
GGTAATC	20	7.023168E-4	45.0	8
AGATATC	20	7.023168E-4	45.0	31
CGAAATG	20	7.023168E-4	45.0	1
CCGATTG	25	3.8824204E-5	45.0	1
TACCAAA	20	7.023168E-4	45.0	35
CGACGAC	20	7.023168E-4	45.0	28
CCTAGCG	25	3.8824204E-5	45.0	1
TAGACAA	25	3.8824204E-5	45.0	39
ACACCAT	20	7.023168E-4	45.0	11
>>END_MODULE