FastQCFastQC Report
Sat 18 Jun 2016
SRR3548397_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3548397_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences387953
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTCTCTTATACACATCTGACGCAAACGACGTCGTATGCCGTCTTCTGCT16980.4376818841457599No Hit
AATGATACCTGTCTCTTATACACATCTGACGCAAACGACGTCGTATGCCGT11250.28998358048526496No Hit
AATGATACGGCTGTCTCTTATACACATCTGACGCAAACGACGTCGTATGCC10630.27400226316074366No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA9250.23843094395455117No Hit
AATCTGTCTCTTATACACATCTGACGCAAACGACGTCGTATGCCGTCTTCT8600.22167633708206924No Hit
TGCAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGG7940.20466396702693368No Hit
GCAGGAGGGAAGGACAAAGAGGTGCAGACAGCACTGGTGGTGGGGTGGAAG5440.14022317136354145No Hit
AAACTGTCTCTTATACACATCTGACGCAAACGACGTCGTATGCCGTCTTCT4530.1167667217420667No Hit
AGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT3940.10155869396550614No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACGTTG207.027502E-445.032
GTGTTAA207.027502E-445.044
CGGAACA253.8860126E-545.027
TCGTTGA351.209155E-745.024
GATCGTG351.209155E-745.010
CGTATAA207.027502E-445.036
GTTTCGA207.027502E-445.030
TCCGATA207.027502E-445.029
GGGTCGA207.027502E-445.08
CGGCCGA207.027502E-445.039
TTTCGCG253.8860126E-545.01
TAGGTGA207.027502E-445.025
TTAGACG207.027502E-445.021
CGTGAAT207.027502E-445.027
TGGCGTG207.027502E-445.032
TTGTCGG253.8860126E-545.02
TAAACGT253.8860126E-545.042
TAAACGA207.027502E-445.022
ATTGTCG253.8860126E-545.01
GATCACG253.8860126E-545.01