##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3548397_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	387953
Sequences flagged as poor quality	0
Sequence length	51
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.964825636095092	33.0	31.0	34.0	30.0	34.0
2	32.161573695782735	34.0	31.0	34.0	30.0	34.0
3	32.29909035372842	34.0	31.0	34.0	30.0	34.0
4	35.923776849257514	37.0	35.0	37.0	35.0	37.0
5	35.725126497281884	37.0	35.0	37.0	35.0	37.0
6	35.73488541137715	37.0	35.0	37.0	33.0	37.0
7	35.77090008325751	37.0	35.0	37.0	35.0	37.0
8	35.81947039976492	37.0	35.0	37.0	35.0	37.0
9	37.5127244795117	39.0	37.0	39.0	35.0	39.0
10	37.101076676813946	39.0	37.0	39.0	33.0	39.0
11	37.11661721909613	39.0	37.0	39.0	33.0	39.0
12	37.006856500658586	39.0	37.0	39.0	33.0	39.0
13	36.99668516547108	39.0	37.0	39.0	33.0	39.0
14	38.209401654324104	40.0	38.0	41.0	33.0	41.0
15	38.24291860096455	40.0	38.0	41.0	33.0	41.0
16	38.124607877758386	40.0	37.0	41.0	33.0	41.0
17	38.11862261665717	40.0	37.0	41.0	33.0	41.0
18	38.065531133926015	40.0	37.0	41.0	33.0	41.0
19	38.09838562918704	40.0	37.0	41.0	33.0	41.0
20	38.011223008972735	40.0	37.0	41.0	33.0	41.0
21	37.96586699935301	40.0	37.0	41.0	33.0	41.0
22	37.92831348127221	40.0	37.0	41.0	33.0	41.0
23	37.80469799176704	40.0	37.0	41.0	33.0	41.0
24	37.67405587790274	40.0	36.0	41.0	33.0	41.0
25	37.69271793232686	40.0	36.0	41.0	33.0	41.0
26	37.62681046415416	40.0	36.0	41.0	33.0	41.0
27	37.53610360017837	40.0	36.0	41.0	33.0	41.0
28	37.43509394179192	40.0	36.0	41.0	32.0	41.0
29	37.283719935146785	39.0	36.0	41.0	32.0	41.0
30	37.300853453897766	39.0	36.0	41.0	32.0	41.0
31	37.241049817890314	39.0	36.0	41.0	32.0	41.0
32	37.15799336517568	39.0	35.0	41.0	32.0	41.0
33	37.0505602482775	39.0	35.0	41.0	31.0	41.0
34	36.987184014558466	39.0	35.0	41.0	31.0	41.0
35	36.90645000811954	39.0	35.0	41.0	31.0	41.0
36	36.79384616177733	39.0	35.0	41.0	31.0	41.0
37	36.72752369488057	39.0	35.0	41.0	31.0	41.0
38	36.629068469634205	39.0	35.0	41.0	31.0	41.0
39	36.5795753609329	39.0	35.0	41.0	30.0	41.0
40	36.44264640304367	39.0	35.0	41.0	30.0	41.0
41	36.342459009209875	39.0	35.0	40.0	30.0	41.0
42	36.26174304619374	39.0	35.0	40.0	30.0	41.0
43	36.17050519006168	38.0	35.0	40.0	30.0	41.0
44	36.088224604526836	38.0	35.0	40.0	30.0	41.0
45	35.90533647117048	38.0	35.0	40.0	29.0	41.0
46	35.734483300812215	38.0	35.0	40.0	28.0	41.0
47	35.58065023340456	38.0	34.0	40.0	28.0	41.0
48	35.52510226754272	38.0	34.0	40.0	28.0	41.0
49	35.345629496356516	37.0	34.0	40.0	28.0	41.0
50	35.22404260309883	37.0	34.0	40.0	27.0	41.0
51	33.988398079148766	36.0	32.0	39.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	1.0
10	2.0
11	6.0
12	7.0
13	7.0
14	16.0
15	28.0
16	49.0
17	76.0
18	157.0
19	308.0
20	522.0
21	766.0
22	1063.0
23	1424.0
24	1787.0
25	2215.0
26	2650.0
27	3333.0
28	4041.0
29	5156.0
30	6682.0
31	8708.0
32	11237.0
33	15163.0
34	23984.0
35	30229.0
36	35547.0
37	51455.0
38	79359.0
39	101952.0
40	22.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	20.96052872384026	31.130317332254165	20.873662531286005	27.03549141261957
2	24.710983031449686	30.918178232930277	19.187118027183704	25.18372070843633
3	21.4799215368872	30.489002533812087	20.547076578863933	27.48399935043678
4	22.36327596384098	30.311403700963773	19.03787314442729	28.287447190767956
5	18.84506628380242	34.48613620722098	16.48472882024369	30.184068688732914
6	19.971233628815863	37.31379832093063	19.892615858106524	22.822352192146987
7	85.36652635757424	6.047124265052725	4.693867556121488	3.8924818212515437
8	86.92186940170588	4.4690980608475765	3.7465878598696234	4.862444677576923
9	80.57496655522705	7.523334012109714	7.130245158563022	4.771454274100213
10	37.05216869053726	33.00064698558846	13.3219745690844	16.625209754789886
11	27.854920570275265	29.542238363925527	22.636504937453765	19.966336128345443
12	27.024923122130772	24.773877248017158	28.861228035354795	19.33997159449727
13	26.887019819411112	24.616899469781135	27.062041020432886	21.434039690374863
14	22.848128510412344	27.544831461543023	28.142326518933995	21.464713509110638
15	20.995842279863798	28.23357468559336	27.597930677169657	23.172652357373185
16	23.54847107768209	27.40486605336213	27.061525494067578	21.985137374888193
17	22.998146682716722	26.053671449892125	26.629256636757546	24.31892523063361
18	25.043239773890136	26.1531680383964	24.946320817212396	23.857271370501067
19	23.879696767391927	30.150817238170603	24.518949460372777	21.450536534064693
20	24.809964093588658	28.447775890378473	23.49227870386361	23.249981312169258
21	25.745644446621114	27.168497214868815	25.171863602034268	21.91399473647581
22	23.97171822359925	26.142599747907607	24.09157810353316	25.794103924959984
23	23.01026155230144	28.334617853193556	24.26840364683351	24.3867169476715
24	22.021739746825002	28.617641827747175	25.3260059852611	24.034612440166722
25	24.49033774709823	27.702324766144354	22.828796271713326	24.97854121504409
26	25.58299587836671	27.66804226285143	23.070835900225028	23.678125958556837
27	22.029988168669917	26.46325714712865	25.692545230994472	25.81420945320696
28	22.425654654043147	28.56815129667769	25.84256340329885	23.163630645980312
29	23.524756864877965	30.583601621845947	23.08423958572301	22.80740192755308
30	24.22716153760894	28.600371694509384	24.667678816763885	22.50478795111779
31	24.964622003180796	29.111773848894067	20.63780921915799	25.285794928767146
32	25.396890860490835	28.682855912958527	22.95613128394419	22.96412194260645
33	23.438406198689016	28.3632295664681	23.363139349354174	24.835224885488707
34	21.122919528912007	29.24400636159535	24.949156212221585	24.68391789727106
35	23.460831595579876	28.64367590919519	24.784445538505953	23.111046956718983
36	24.081783102592325	30.003634460875418	23.517797258946317	22.396785177585947
37	23.62915095385266	29.801032599309714	21.634837209661995	24.934979237175636
38	24.429247872809334	28.96587988751215	23.253332233543755	23.351540006134762
39	23.738442543297772	25.49355205398592	23.97171822359925	26.796287179117055
40	24.539054988619753	25.972476047356253	24.280260753235574	25.20820821078842
41	22.5813436163659	24.867187520137747	26.74447677940369	25.806992084092663
42	24.299335228751936	27.19014932221171	23.82144228811222	24.68907316092413
43	22.676973757130376	26.101357638683037	25.169543733390388	26.052124870796206
44	24.120705343173015	24.22819259033955	24.92569976260011	26.725402303887325
45	26.000829997448143	24.939361211280747	25.37008348949486	23.689725301776246
46	22.78136784610507	26.125587377852472	26.90171232082237	24.191332455220092
47	21.924563026964606	28.21269586779842	25.451536655213392	24.411204450023586
48	22.858181274535834	25.81240511092839	26.937799166393866	24.39161444814191
49	23.6033746355873	25.759563658484403	26.19750330581282	24.439558400115477
50	22.214031081084563	25.947988545004165	26.68029374692295	25.15768662698832
51	23.797212548942785	23.929187298461414	26.909187453119323	25.364412699476485
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1257.0
1	1010.5
2	764.0
3	532.5
4	301.0
5	315.5
6	330.0
7	338.0
8	346.0
9	378.0
10	410.0
11	412.5
12	415.0
13	484.5
14	554.0
15	538.0
16	522.0
17	543.0
18	564.0
19	614.5
20	665.0
21	638.0
22	611.0
23	1038.0
24	1465.0
25	1669.5
26	2119.0
27	2364.0
28	3159.0
29	3954.0
30	4784.0
31	5614.0
32	6282.0
33	6950.0
34	7887.5
35	8825.0
36	10053.5
37	11282.0
38	11959.0
39	12636.0
40	13835.5
41	15035.0
42	16055.5
43	17076.0
44	20461.0
45	23846.0
46	26571.5
47	29297.0
48	31878.0
49	34459.0
50	35074.0
51	35689.0
52	32930.0
53	30171.0
54	27441.0
55	24711.0
56	23487.0
57	22263.0
58	20777.0
59	19291.0
60	18902.5
61	18514.0
62	17127.0
63	15740.0
64	13711.0
65	11682.0
66	9702.0
67	7722.0
68	6927.5
69	6133.0
70	5603.0
71	5073.0
72	4573.0
73	4073.0
74	3315.0
75	1920.0
76	1283.0
77	1063.0
78	843.0
79	632.5
80	422.0
81	311.0
82	200.0
83	151.5
84	103.0
85	69.5
86	36.0
87	26.0
88	16.0
89	12.0
90	8.0
91	5.5
92	3.0
93	2.0
94	1.0
95	1.0
96	1.0
97	1.0
98	1.0
99	1.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	387953.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	24.6542751312659
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.69611174422616	19.155413155717316
2	8.575282026618712	4.228347248249144
3	3.041391784373791	2.2494992950176953
4	1.4762616705176326	1.455846455627357
5	0.8803203446004579	1.085182998971525
6	0.6461258586259894	0.9557858812794332
7	0.4621159053603354	0.7975192871301421
8	0.3805660397085115	0.7506063878871925
9	0.31783537382249316	0.7052400677401643
>10	4.558428387717336	27.715212925277033
>50	1.6215877131535752	28.209602709606575
>100	0.33665457358829864	10.88508144027756
>500	0.0041820443924012254	0.8049944194270955
>1k	0.003136533294300919	1.0016677277917685
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTGTCTCTTATACACATCTGACGCAAACGACGTCGTATGCCGTCTTCTGCT	1698	0.4376818841457599	No Hit
AATGATACCTGTCTCTTATACACATCTGACGCAAACGACGTCGTATGCCGT	1125	0.28998358048526496	No Hit
AATGATACGGCTGTCTCTTATACACATCTGACGCAAACGACGTCGTATGCC	1063	0.27400226316074366	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	925	0.23843094395455117	No Hit
AATCTGTCTCTTATACACATCTGACGCAAACGACGTCGTATGCCGTCTTCT	860	0.22167633708206924	No Hit
TGCAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGG	794	0.20466396702693368	No Hit
GCAGGAGGGAAGGACAAAGAGGTGCAGACAGCACTGGTGGTGGGGTGGAAG	544	0.14022317136354145	No Hit
AAACTGTCTCTTATACACATCTGACGCAAACGACGTCGTATGCCGTCTTCT	453	0.1167667217420667	No Hit
AGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT	394	0.10155869396550614	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.5083089961928378	0.0
2	0.0	0.0	0.0	0.6766283544656183	0.0
3	0.0	0.0	0.0	0.8810345583098984	0.0
4	0.0	0.0	0.0	1.9726616368477625	0.0
5	0.0	0.0	0.0	2.142269811033811	0.0
6	0.0	0.0	0.0	3.045987529417223	0.0
7	0.0	0.0	0.0	3.6973550919827916	0.0
8	0.0	0.0	0.0	4.3541356813840855	0.0
9	0.0	0.0	0.0	5.253986952027694	0.0
10	0.0	0.0	0.0	6.335303503259415	0.0
11	0.0	0.0	0.0	7.38362636711148	0.0
12	0.0	0.0	0.0	7.859457202289968	0.0
13	0.0	0.0	0.0	8.072369591161817	0.0
14	0.0	0.0	0.0	8.320337772874549	0.0
15	0.0	0.0	0.0	8.517526607604529	0.0
16	0.0	0.0	0.0	8.940515990339035	0.0
17	0.0	0.0	0.0	9.404747482298113	0.0
18	0.0	0.0	0.0	10.27701809239779	0.0
19	0.0	0.0	0.0	10.517253378630917	0.0
20	0.0	0.0	0.0	10.871677754779574	0.0
21	0.0	0.0	0.0	11.15882594025565	0.0
22	0.0	0.0	0.0	11.454738073941947	0.0
23	0.0	0.0	0.0	11.77410665724972	0.0
24	0.0	0.0	0.0	12.017692864857342	0.0
25	0.0	0.0	0.0	12.221325779153661	0.0
26	0.0	0.0	0.0	12.406399744298923	0.0
27	0.0	0.0	0.0	12.671638059249446	0.0
28	0.0	0.0	0.0	12.887128079947828	0.0
29	0.0	0.0	0.0	13.123754681623804	0.0
30	0.0	0.0	0.0	13.488747348261258	0.0
31	0.0	0.0	0.0	13.708103816699445	0.0
32	0.0	0.0	0.0	13.938801865174389	0.0
33	0.0	0.0	0.0	14.171819782293216	0.0
34	0.0	0.0	0.0	14.401486778037546	0.0
35	0.0	0.0	0.0	14.646104038375782	0.0
36	0.0	0.0	0.0	14.87267787592827	0.0
37	0.0	0.0	0.0	15.078630658868471	0.0
38	0.0	0.0	0.0	15.30958647052607	0.0
39	0.0	0.0	0.0	15.545182019471431	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AACGTTG	20	7.027502E-4	45.0	32
GTGTTAA	20	7.027502E-4	45.0	44
CGGAACA	25	3.8860126E-5	45.0	27
TCGTTGA	35	1.209155E-7	45.0	24
GATCGTG	35	1.209155E-7	45.0	10
CGTATAA	20	7.027502E-4	45.0	36
GTTTCGA	20	7.027502E-4	45.0	30
TCCGATA	20	7.027502E-4	45.0	29
GGGTCGA	20	7.027502E-4	45.0	8
CGGCCGA	20	7.027502E-4	45.0	39
TTTCGCG	25	3.8860126E-5	45.0	1
TAGGTGA	20	7.027502E-4	45.0	25
TTAGACG	20	7.027502E-4	45.0	21
CGTGAAT	20	7.027502E-4	45.0	27
TGGCGTG	20	7.027502E-4	45.0	32
TTGTCGG	25	3.8860126E-5	45.0	2
TAAACGT	25	3.8860126E-5	45.0	42
TAAACGA	20	7.027502E-4	45.0	22
ATTGTCG	25	3.8860126E-5	45.0	1
GATCACG	25	3.8860126E-5	45.0	1
>>END_MODULE