FastQCFastQC Report
Sat 18 Jun 2016
SRR3548396_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3548396_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences334586
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTCTCTTATACACATCTGACGCACTAGCACTCGTATGCCGTCTTCTGCT20620.6162840047102986TruSeq Adapter, Index 13 (95% over 22bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA15850.4737197611376447No Hit
AATGATACCTGTCTCTTATACACATCTGACGCACTAGCACTCGTATGCCGT9880.295290299056147No Hit
AATCTGTCTCTTATACACATCTGACGCACTAGCACTCGTATGCCGTCTTCT9480.28333522621986573No Hit
AATGATACGGCTGTCTCTTATACACATCTGACGCACTAGCACTCGTATGCC9370.2800475811898884No Hit
AAACTGTCTCTTATACACATCTGACGCACTAGCACTCGTATGCCGTCTTCT7400.22116884747120322No Hit
AAAAACTGTCTCTTATACACATCTGACGCACTAGCACTCGTATGCCGTCTT6620.19785645544045477No Hit
TGCAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGG4610.13778221443814148No Hit
CCTGTCTCTTATACACATCTGACGCACTAGCACTCGTATGCCGTCTTCTGC4300.12851703299002348TruSeq Adapter, Index 16 (95% over 21bp)
GCCCAGGGGCTGTCTCTTATACACATCTGACGCACTAGCACTCGTATGCCG4140.12373500385551099No Hit
GCCCAGGGGAATGATACGGCTGTCTCTTATACACATCTGACGCACTAGCAC4010.11984960518371958No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3610.10789453234743833No Hit
GCCCAGGGGAATGATACCTGTCTCTTATACACATCTGACGCACTAGCACTC3570.1066990250638102No Hit
AAAAAACTGTCTCTTATACACATCTGACGCACTAGCACTCGTATGCCGTCT3460.10341138003383285No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCAAC302.160872E-645.00000416
TCTTACG302.160872E-645.0000041
TCTAGCG302.160872E-645.0000041
CCATGCG302.160872E-645.0000041
AAGCACG302.160872E-645.0000041
GCAATAG302.160872E-645.0000041
ATACGCG302.160872E-645.0000041
TTCGCAA302.160872E-645.00000415
AAGTATA207.0261094E-445.042
TATCACG207.0261094E-445.01
TAATAGG750.045.02
CGGAAGA253.884858E-545.01
TGATAGG253.884858E-545.02
ATGATTC253.884858E-545.011
ACGTTTA207.0261094E-445.028
ACGCATG207.0261094E-445.01
CGCGGAT253.884858E-545.027
TTTCGGT207.0261094E-445.026
TTCACGG207.0261094E-445.02
ATAGCGA253.884858E-545.011
Produced by FastQC (version 0.11.5)