##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3548396_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	334586
Sequences flagged as poor quality	0
Sequence length	51
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.712603037784007	31.0	31.0	34.0	30.0	34.0
2	31.914664690094625	33.0	31.0	34.0	30.0	34.0
3	32.09154895901203	34.0	31.0	34.0	30.0	34.0
4	35.76854680112139	37.0	35.0	37.0	35.0	37.0
5	35.50788138176732	37.0	35.0	37.0	33.0	37.0
6	34.85337402043122	37.0	35.0	37.0	32.0	37.0
7	35.25988236208329	37.0	35.0	37.0	33.0	37.0
8	35.64024495944241	37.0	35.0	37.0	33.0	37.0
9	37.23550297980191	39.0	37.0	39.0	34.0	39.0
10	36.8698421332632	39.0	37.0	39.0	32.0	39.0
11	36.90021100703556	39.0	37.0	39.0	33.0	39.0
12	36.793562791031306	39.0	35.0	39.0	33.0	39.0
13	36.766200618077264	39.0	35.0	39.0	33.0	39.0
14	37.90776063553167	40.0	37.0	41.0	33.0	41.0
15	37.958408301602574	40.0	37.0	41.0	33.0	41.0
16	37.84253375813692	40.0	37.0	41.0	33.0	41.0
17	37.79545169253944	39.0	37.0	41.0	33.0	41.0
18	37.755503816657004	39.0	37.0	41.0	33.0	41.0
19	37.71558582845666	40.0	36.0	41.0	33.0	41.0
20	37.62167873132767	39.0	36.0	41.0	33.0	41.0
21	37.585538546143596	39.0	36.0	41.0	32.0	41.0
22	37.573855451214335	39.0	36.0	41.0	32.0	41.0
23	37.428670057922325	39.0	36.0	41.0	32.0	41.0
24	37.329428009540145	39.0	36.0	41.0	32.0	41.0
25	37.299659878177806	39.0	36.0	41.0	32.0	41.0
26	37.30172810577849	39.0	36.0	41.0	32.0	41.0
27	37.21014925908436	39.0	35.0	41.0	32.0	41.0
28	37.016196134924954	39.0	35.0	41.0	31.0	41.0
29	36.80283096124763	39.0	35.0	41.0	31.0	41.0
30	36.81321991954236	39.0	35.0	41.0	31.0	41.0
31	36.73655801497971	39.0	35.0	41.0	31.0	41.0
32	36.693639303497456	39.0	35.0	40.0	31.0	41.0
33	36.439020162230335	39.0	35.0	40.0	30.0	41.0
34	36.36633929692216	39.0	35.0	40.0	30.0	41.0
35	36.360920660159124	39.0	35.0	40.0	30.0	41.0
36	36.32216530279211	39.0	35.0	40.0	30.0	41.0
37	36.24259831553024	39.0	35.0	40.0	30.0	41.0
38	36.03490283514552	38.0	35.0	40.0	29.0	41.0
39	35.972300096238335	38.0	35.0	40.0	29.0	41.0
40	35.87273526088958	38.0	35.0	40.0	29.0	41.0
41	35.83266185674236	38.0	35.0	40.0	29.0	41.0
42	35.752117542276125	38.0	35.0	40.0	29.0	41.0
43	35.70655974846527	38.0	35.0	40.0	29.0	41.0
44	35.60300191878919	38.0	35.0	40.0	28.0	41.0
45	35.33665783983789	38.0	34.0	40.0	28.0	41.0
46	35.18921891531624	37.0	34.0	40.0	27.0	41.0
47	35.04768280800751	37.0	34.0	40.0	27.0	41.0
48	34.96894669830776	37.0	34.0	40.0	26.0	41.0
49	34.876238097230605	37.0	34.0	40.0	26.0	41.0
50	34.763567513285075	37.0	34.0	40.0	26.0	41.0
51	33.539729695803175	36.0	32.0	39.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	0.0
12	1.0
13	11.0
14	11.0
15	25.0
16	45.0
17	85.0
18	215.0
19	385.0
20	662.0
21	1081.0
22	1440.0
23	1808.0
24	2102.0
25	2468.0
26	2758.0
27	3384.0
28	4002.0
29	5100.0
30	6441.0
31	8298.0
32	10796.0
33	14974.0
34	22549.0
35	28499.0
36	33369.0
37	47257.0
38	66187.0
39	70608.0
40	24.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	22.211628699347852	31.34799423765489	19.779966884448243	26.660410178549014
2	23.761902769392623	31.14983890539353	18.410812167873132	26.67744615734071
3	20.495776870520583	31.005780277716344	20.105144865595094	28.39329798616798
4	20.910916774760448	32.10624473229603	18.208173683298163	28.77466480964535
5	17.39552760725195	36.80638161788001	17.1555295200636	28.642561254804445
6	20.639536621376866	38.16029361658886	18.2225197707017	22.977649991332573
7	82.50554416502783	6.901962425206075	5.727077642220535	4.865415767545564
8	83.31310933511863	5.6018482542604895	4.921006856234271	6.164035554386615
9	76.4559784330486	8.946877633851983	9.351257972539198	5.245885960560215
10	34.936309349464715	33.30324640002869	13.500863754012421	18.259580496494173
11	26.416825569509783	29.94387093303366	24.35517325889308	19.284130238563478
12	25.691451525168418	23.754430848869948	30.58107631520745	19.973041310754187
13	26.04621831158506	25.08831810057803	27.01308482721931	21.8523787606176
14	19.394415785478174	29.380787002444812	27.58603169289809	23.638765519178925
15	18.519603330683292	28.9040784730981	29.45281631628341	23.123501879935205
16	20.93811456546299	28.79917270895973	26.560884197186972	23.70182852839031
17	20.266837225705796	27.280280705110194	27.335871793798905	25.117010275385105
18	24.192584268319656	25.42963543005386	26.23182081736833	24.14595948425816
19	22.75857328160772	30.66864722373321	24.810362657134487	21.762416837524583
20	24.16389209351258	28.201120190324758	23.41072250482686	24.224265211335798
21	23.609774467550942	28.950105503517783	24.36653057808755	23.07358945084373
22	21.433054580885035	26.983197145128607	23.850071431560195	27.733676842426163
23	22.74452607102509	28.380745159689884	22.98811068006432	25.886618089220708
24	20.512812849312283	28.667069154118824	24.58441178052877	26.23570621604012
25	21.575319947636782	29.49884334670309	24.215000029887683	24.710836675772445
26	22.64260907509579	26.5662639799633	24.158213433915346	26.632913511025563
27	20.667631042542126	26.261409622638126	25.17170473361109	27.899254601208657
28	20.597394989628974	29.85958766953788	26.259915238533594	23.28310210229956
29	23.391295511467906	29.207737323139643	21.711308901149483	25.689658264242976
30	22.840465530536243	27.01398145768203	25.095790021100704	25.049762990681018
31	23.791790451483326	30.00125528264781	20.473659985773462	25.733294280095404
32	25.325028542736394	25.284381295093038	23.46511808623194	25.92547207593862
33	21.0788855481102	27.776117350994962	23.03204557273765	28.112951528157186
34	21.376865738554514	29.478220846060506	23.94541313742954	25.199500277955444
35	21.44381414643769	26.450598650272276	24.551236453408094	27.554350749881944
36	22.5663954857645	29.848828103985227	25.39705785657499	22.187718553675285
37	22.39394356010114	29.408582546789162	22.432498669998147	25.76497522311155
38	23.73918813100369	28.319475411403943	23.815102843514076	24.126233614078295
39	23.966932268534848	25.30560154937744	22.755883390219555	27.971582791868162
40	24.21201126167861	26.134386973752637	24.14267183922818	25.51092992534057
41	20.989521378659	23.904467012965277	26.55191789255976	28.554093715815966
42	23.41699891806591	26.219267990890234	24.351885613863104	26.011847477180755
43	21.693077415074153	24.142970716049085	26.84392054658593	27.320031322290834
44	24.022224480402645	24.243094451052944	24.752978307520337	26.981702761024074
45	24.290018111935346	24.26879785765095	26.230326433263794	25.21085759714991
46	22.46836388850699	23.806136538886864	27.980250219674463	25.745249352931683
47	21.92560358173982	26.343002994745746	26.11495998039368	25.61643344312075
48	23.110052422994386	24.66510852217367	27.573777743240903	24.65106131159104
49	22.64470121284214	23.23558068777534	26.707632716252323	27.412085383130197
50	21.494324329170976	24.05031890156791	26.48227959328842	27.973077175972694
51	22.92923194634563	22.51289653482214	27.581249663763575	26.97662185506865
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1903.0
1	1513.5
2	1124.0
3	805.0
4	486.0
5	495.0
6	504.0
7	540.0
8	576.0
9	614.0
10	652.0
11	767.5
12	883.0
13	941.0
14	999.0
15	982.5
16	966.0
17	867.5
18	769.0
19	802.5
20	836.0
21	899.0
22	962.0
23	1131.0
24	1300.0
25	1271.0
26	1556.0
27	1870.0
28	2758.5
29	3647.0
30	3531.5
31	3416.0
32	4209.0
33	5002.0
34	5225.0
35	5448.0
36	6752.0
37	8056.0
38	8728.0
39	9400.0
40	10097.5
41	10795.0
42	12819.5
43	14844.0
44	17795.0
45	20746.0
46	23676.0
47	26606.0
48	28935.5
49	31265.0
50	31567.5
51	31870.0
52	29136.0
53	26402.0
54	25332.5
55	24263.0
56	21264.5
57	18266.0
58	16840.5
59	15415.0
60	14963.0
61	14511.0
62	13368.0
63	12225.0
64	11386.0
65	10547.0
66	8964.5
67	7382.0
68	6538.0
69	5694.0
70	5216.0
71	4738.0
72	3967.5
73	3197.0
74	2781.0
75	1844.5
76	1324.0
77	1170.0
78	1016.0
79	705.5
80	395.0
81	363.0
82	331.0
83	272.0
84	213.0
85	139.0
86	65.0
87	45.0
88	25.0
89	18.5
90	12.0
91	16.5
92	21.0
93	14.5
94	8.0
95	5.5
96	3.0
97	1.5
98	0.0
99	0.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	334586.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	30.613096963631087
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.41088388488045	23.391737974446013
2	9.557581918947905	5.851743640451989
3	3.4995527283561567	3.213964410075202
4	1.7180672176402407	2.1038143329462624
5	1.077380810703416	1.6490981612409572
6	0.7355754612225542	1.3510945751084227
7	0.48272130647698297	1.034431591311339
8	0.4477414952708396	1.0965403047493907
9	0.3567940036540955	0.9830312486914473
>10	4.700286271804018	34.22864019216158
>50	0.9194691409860704	18.280159054309074
>100	0.08694979920194361	4.391974345996632
>500	0.004997114896663426	1.3079547425381821
>1k	0.00199884595866537	1.1158154259735087
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTGTCTCTTATACACATCTGACGCACTAGCACTCGTATGCCGTCTTCTGCT	2062	0.6162840047102986	TruSeq Adapter, Index 13 (95% over 22bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1585	0.4737197611376447	No Hit
AATGATACCTGTCTCTTATACACATCTGACGCACTAGCACTCGTATGCCGT	988	0.295290299056147	No Hit
AATCTGTCTCTTATACACATCTGACGCACTAGCACTCGTATGCCGTCTTCT	948	0.28333522621986573	No Hit
AATGATACGGCTGTCTCTTATACACATCTGACGCACTAGCACTCGTATGCC	937	0.2800475811898884	No Hit
AAACTGTCTCTTATACACATCTGACGCACTAGCACTCGTATGCCGTCTTCT	740	0.22116884747120322	No Hit
AAAAACTGTCTCTTATACACATCTGACGCACTAGCACTCGTATGCCGTCTT	662	0.19785645544045477	No Hit
TGCAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGG	461	0.13778221443814148	No Hit
CCTGTCTCTTATACACATCTGACGCACTAGCACTCGTATGCCGTCTTCTGC	430	0.12851703299002348	TruSeq Adapter, Index 16 (95% over 21bp)
GCCCAGGGGCTGTCTCTTATACACATCTGACGCACTAGCACTCGTATGCCG	414	0.12373500385551099	No Hit
GCCCAGGGGAATGATACGGCTGTCTCTTATACACATCTGACGCACTAGCAC	401	0.11984960518371958	No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	361	0.10789453234743833	No Hit
GCCCAGGGGAATGATACCTGTCTCTTATACACATCTGACGCACTAGCACTC	357	0.1066990250638102	No Hit
AAAAAACTGTCTCTTATACACATCTGACGCACTAGCACTCGTATGCCGTCT	346	0.10341138003383285	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.6796458907425893	0.0
2	0.0	0.0	0.0	0.9853968785304824	0.0
3	0.0	0.0	0.0	1.370051347037832	0.0
4	0.0	0.0	0.0	3.057211001058024	0.0
5	0.0	0.0	0.0	3.365054126592266	0.0
6	0.0	0.0	0.0	4.876773086740031	0.0
7	0.0	0.0	0.0	5.9766397876779065	0.0
8	0.0	0.0	0.0	7.123729026319093	0.0
9	0.0	0.0	0.0	8.538014142851166	0.0
10	0.0	0.0	0.0	10.737747544726917	0.0
11	0.0	0.0	0.0	12.269491251875452	0.0
12	0.0	0.0	0.0	13.065699102771784	0.0
13	0.0	0.0	0.0	13.444674911681899	0.0
14	0.0	0.0	0.0	13.828432749726527	0.0
15	0.0	0.0	0.0	14.235204102980997	0.0
16	0.0	0.0	0.0	14.844912817631341	0.0
17	0.0	0.0	0.0	15.650684726796698	0.0
18	0.0	0.0	0.0	16.87787295344097	0.0
19	0.0	0.0	0.0	17.264918436515575	0.0
20	0.0	0.0	0.0	17.91886092066016	0.0
21	0.0	0.0	0.0	18.33071317987005	0.0
22	0.0	0.0	0.0	18.753623881453496	0.0
23	0.0	0.0	0.0	19.18221324263418	0.0
24	0.0	0.0	0.0	19.501114810541985	0.0
25	0.0	0.0	0.0	19.776978116239174	0.0
26	0.0	0.0	0.0	20.037598704070106	0.0
27	0.0	0.0	0.0	20.416275636159313	0.0
28	0.0	0.0	0.0	20.716049087529065	0.0
29	0.0	0.0	0.0	21.033456271332334	0.0
30	0.0	0.0	0.0	21.59952897013025	0.0
31	0.0	0.0	0.0	21.88854285594735	0.0
32	0.0	0.0	0.0	22.162015147077284	0.0
33	0.0	0.0	0.0	22.465673997118827	0.0
34	0.0	0.0	0.0	22.73914628824876	0.0
35	0.0	0.0	0.0	23.039816370081233	0.0
36	0.0	0.0	0.0	23.29595380559856	0.0
37	0.0	0.0	0.0	23.526088957696974	0.0
38	0.0	0.0	0.0	23.801653386573257	0.0
39	0.0	0.0	0.0	24.10053020748029	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGCAAC	30	2.160872E-6	45.000004	16
TCTTACG	30	2.160872E-6	45.000004	1
TCTAGCG	30	2.160872E-6	45.000004	1
CCATGCG	30	2.160872E-6	45.000004	1
AAGCACG	30	2.160872E-6	45.000004	1
GCAATAG	30	2.160872E-6	45.000004	1
ATACGCG	30	2.160872E-6	45.000004	1
TTCGCAA	30	2.160872E-6	45.000004	15
AAGTATA	20	7.0261094E-4	45.0	42
TATCACG	20	7.0261094E-4	45.0	1
TAATAGG	75	0.0	45.0	2
CGGAAGA	25	3.884858E-5	45.0	1
TGATAGG	25	3.884858E-5	45.0	2
ATGATTC	25	3.884858E-5	45.0	11
ACGTTTA	20	7.0261094E-4	45.0	28
ACGCATG	20	7.0261094E-4	45.0	1
CGCGGAT	25	3.884858E-5	45.0	27
TTTCGGT	20	7.0261094E-4	45.0	26
TTCACGG	20	7.0261094E-4	45.0	2
ATAGCGA	25	3.884858E-5	45.0	11
>>END_MODULE