##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3548394_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	547210
Sequences flagged as poor quality	0
Sequence length	51
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.650063047093436	31.0	31.0	34.0	30.0	34.0
2	31.856093638639646	33.0	31.0	34.0	30.0	34.0
3	32.034703313170446	33.0	31.0	34.0	30.0	34.0
4	35.71822517863343	37.0	35.0	37.0	33.0	37.0
5	35.475665649385064	37.0	35.0	37.0	33.0	37.0
6	34.865850404780616	37.0	35.0	37.0	32.0	37.0
7	35.243387364996984	37.0	35.0	37.0	33.0	37.0
8	35.6233859030354	37.0	35.0	37.0	33.0	37.0
9	37.16027484877835	39.0	37.0	39.0	34.0	39.0
10	36.83312622210851	39.0	37.0	39.0	32.0	39.0
11	36.85768352186546	39.0	37.0	39.0	33.0	39.0
12	36.78524880758758	39.0	35.0	39.0	33.0	39.0
13	36.714210266625244	39.0	35.0	39.0	32.0	39.0
14	37.84613037042452	40.0	37.0	41.0	33.0	41.0
15	37.881471464337274	40.0	37.0	41.0	33.0	41.0
16	37.76058368816359	40.0	36.0	41.0	33.0	41.0
17	37.72674293232945	39.0	36.0	41.0	33.0	41.0
18	37.66458032565194	39.0	36.0	41.0	33.0	41.0
19	37.6336433910199	39.0	36.0	41.0	32.0	41.0
20	37.46794283730195	39.0	36.0	41.0	32.0	41.0
21	37.42348824034649	39.0	36.0	41.0	32.0	41.0
22	37.380075291021726	39.0	36.0	41.0	32.0	41.0
23	37.25848394583432	39.0	35.0	41.0	32.0	41.0
24	37.143105937391496	39.0	35.0	41.0	31.0	41.0
25	37.06123060616582	39.0	35.0	41.0	31.0	41.0
26	37.11076734708796	39.0	35.0	41.0	31.0	41.0
27	36.99036750059392	39.0	35.0	41.0	31.0	41.0
28	36.78682955355348	39.0	35.0	41.0	31.0	41.0
29	36.51257652455182	39.0	35.0	40.0	30.0	41.0
30	36.60605434842199	39.0	35.0	40.0	30.0	41.0
31	36.52543082180516	39.0	35.0	40.0	30.0	41.0
32	36.421986074815884	39.0	35.0	40.0	30.0	41.0
33	36.19961075272747	39.0	35.0	40.0	30.0	41.0
34	36.15721386670565	39.0	35.0	40.0	30.0	41.0
35	36.094731455931	39.0	35.0	40.0	29.0	41.0
36	36.05206045211162	39.0	35.0	40.0	29.0	41.0
37	35.92718700316149	38.0	35.0	40.0	29.0	41.0
38	35.70832587123773	38.0	35.0	40.0	28.0	41.0
39	35.64888068565998	38.0	35.0	40.0	28.0	41.0
40	35.548909924891724	38.0	35.0	40.0	27.0	41.0
41	35.5266479048263	38.0	35.0	40.0	27.0	41.0
42	35.40920670309388	38.0	35.0	40.0	27.0	41.0
43	35.34957511741379	38.0	34.0	40.0	27.0	41.0
44	35.240182014217574	38.0	34.0	40.0	26.0	41.0
45	35.023128232305694	38.0	34.0	40.0	26.0	41.0
46	34.87224831417554	37.0	34.0	40.0	25.0	41.0
47	34.74023318287312	37.0	34.0	40.0	25.0	41.0
48	34.61075272747208	37.0	34.0	40.0	24.0	41.0
49	34.46824436687926	37.0	33.0	40.0	24.0	41.0
50	34.385183019316166	37.0	33.0	40.0	24.0	41.0
51	33.16266332852104	35.0	31.0	39.0	21.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	2.0
10	7.0
11	6.0
12	13.0
13	16.0
14	23.0
15	45.0
16	93.0
17	187.0
18	407.0
19	762.0
20	1461.0
21	2292.0
22	3086.0
23	3845.0
24	4332.0
25	4734.0
26	5289.0
27	6134.0
28	7217.0
29	9115.0
30	11154.0
31	14173.0
32	18390.0
33	24543.0
34	37272.0
35	46335.0
36	52284.0
37	73613.0
38	105663.0
39	114694.0
40	23.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	20.063778074231102	32.05405602967782	20.60360739021582	27.278558505875257
2	23.768023245189234	34.13314815153232	17.993823212295094	24.105005390983354
3	22.281756546846733	32.04802543813161	19.48319657900989	26.18702143601177
4	20.89727892399627	31.859615138612234	17.627053599166683	29.61605233822481
5	17.63326693591126	36.50865298514282	17.554321010215457	28.30375906873047
6	19.28619725516712	38.06198717128708	18.544617240182014	24.10719833336379
7	81.75727782752507	7.686811279033644	5.89115695985088	4.664753933590395
8	82.87184079238318	6.161985343835091	4.956780760585516	6.009393103196214
9	76.12105041940023	9.787284589097421	9.103086566400467	4.98857842510188
10	32.43599349427094	35.700370972752694	13.505783885528407	18.357851647447962
11	25.666745856252625	32.522797463496644	23.630050620419947	18.18040605983078
12	26.807624129675993	23.729098517936443	29.824016373969776	19.63926097841779
13	27.142596078288044	25.26689936221926	26.270353246468446	21.320151313024247
14	20.349774309680015	29.734471226768516	28.182781747409585	21.732972716141884
15	19.529065623800733	29.58224447652638	30.387602565742583	20.5010873339303
16	20.991027210760038	29.6434641179803	26.01578918513916	23.349719486120502
17	19.563787211491018	27.057802306244405	27.031121507282396	26.34728897498218
18	23.5914913835639	26.496043566455292	25.95164562051132	23.960819429469492
19	24.642824509786003	30.773012189104733	23.897406845635132	20.686756455474132
20	23.448584638438625	28.124303283931212	24.432850276858975	23.994261800771184
21	24.022952796915263	29.762979477714225	24.63021509109848	21.583852634272034
22	20.396922570859452	29.57822408216224	24.292684709709253	25.732168637269055
23	22.651632828347434	27.962756528572214	23.64741141426509	25.73819922881526
24	20.706492936898083	28.207269603991154	24.227444673891192	26.85879278521957
25	21.47073335648106	29.239414484384422	23.682863982748852	25.606988176385663
26	21.80625354068822	28.19831508927103	24.463916960581862	25.531514409458893
27	19.818716763217044	27.506259023044173	24.353721605964804	28.32130260777398
28	22.251969079512435	27.222821220372435	26.18665594561503	24.3385537545001
29	22.564463368724986	29.28363882238994	24.050547321869118	24.101350487015953
30	22.674475978143676	26.382924288664316	24.730359459805193	26.212240273386815
31	23.956616289906982	26.770344109208533	23.8113338572029	25.461705743681584
32	23.132069954861937	25.743864329964733	25.039929825843828	26.084135889329506
33	23.220335885674604	25.21828913945286	26.220829297710203	25.340545677162336
34	21.287805412912775	24.322289431845178	28.557409404067908	25.83249575117414
35	22.858317647703807	23.96008844867601	27.749858372471266	25.43173553114892
36	22.68763363242631	25.91911697520148	28.186071160980248	23.20717823139197
37	24.098061073445294	25.614480729518828	25.983626030226052	24.303832166809816
38	24.90634308583542	24.52787778001133	28.580983534657626	21.984795599495623
39	24.159828950494326	22.447689186966613	28.44392463588019	24.948557226658867
40	23.790500904588733	23.134811132837484	28.62776630544032	24.44692165713346
41	21.904204967014493	22.15730706675682	30.50730067067488	25.43118729555381
42	24.452952248679665	24.29432941649458	25.722483141755447	25.5302351930703
43	21.796019809579505	23.699493795800517	27.979020851227133	26.525465543392844
44	24.7382175033351	21.085323733118912	26.216443412949324	27.960015350596663
45	25.87215145922041	21.931251256373237	27.90720198826776	24.289395296138593
46	21.122786498784745	23.484037206922387	30.988651523181225	24.40452477111164
47	22.757625043401987	24.1866924946547	27.905922771879172	25.149759690064144
48	22.49118254417865	22.731857970431825	31.14599513897772	23.630964346411798
49	23.694376930246158	22.05204583249575	29.527786407412144	24.725790829845945
50	22.232415343286853	21.618574221962316	27.763564262349007	28.385446172401817
51	22.23917691562654	21.25472853200782	30.92615266533872	25.57994188702692
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	3694.0
1	2964.5
2	2235.0
3	1600.0
4	965.0
5	972.0
6	979.0
7	1110.5
8	1242.0
9	1289.0
10	1336.0
11	1458.5
12	1581.0
13	1802.0
14	2023.0
15	1882.0
16	1741.0
17	1766.0
18	1791.0
19	1759.5
20	1728.0
21	1667.0
22	1606.0
23	1901.5
24	2197.0
25	2649.0
26	3524.0
27	3947.0
28	4622.0
29	5297.0
30	6112.5
31	6928.0
32	7626.5
33	8325.0
34	9033.5
35	9742.0
36	11032.5
37	12323.0
38	14104.5
39	15886.0
40	16438.0
41	16990.0
42	20444.0
43	23898.0
44	28973.5
45	34049.0
46	38745.0
47	43441.0
48	48997.0
49	54553.0
50	53048.5
51	51544.0
52	47198.5
53	42853.0
54	39611.5
55	36370.0
56	33083.0
57	29796.0
58	28084.5
59	26373.0
60	25366.0
61	24359.0
62	22139.5
63	19920.0
64	17407.0
65	14894.0
66	12841.0
67	10788.0
68	9400.5
69	8013.0
70	6821.5
71	5630.0
72	5328.0
73	5026.0
74	4263.5
75	3185.0
76	2869.0
77	2170.0
78	1471.0
79	1275.5
80	1080.0
81	803.5
82	527.0
83	370.5
84	214.0
85	186.0
86	158.0
87	152.0
88	146.0
89	107.5
90	69.0
91	36.5
92	4.0
93	3.5
94	3.0
95	3.5
96	4.0
97	2.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	547210.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	29.244219268901062
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.659667777314	22.418521335608794
2	10.050884157963752	5.87860520323632
3	3.755198020514798	3.2945350293023394
4	1.889720874202247	2.210536464087596
5	1.1164021542973128	1.6324154696272062
6	0.7249393595859706	1.27201713530333
7	0.5029438097640625	1.0295739336873693
8	0.3656699905876863	0.8554986707842627
9	0.28325412611539585	0.7455191195645633
>10	3.2811496251641223	22.771934959974985
>50	1.0603869843017775	21.992493346987555
>100	0.29896933570596007	12.009146983704454
>500	0.005088839756697193	1.075776399255896
>1k	0.005724944726284342	2.813425948875353
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTGTCTCTTATACACATCTGACGCCTCGTTTGTCGTATGCCGTCTTCTGCT	3269	0.597394053471245	TruSeq Adapter, Index 27 (95% over 23bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	3066	0.5602967782021527	No Hit
AAACTGTCTCTTATACACATCTGACGCCTCGTTTGTCGTATGCCGTCTTCT	1361	0.24871621498145136	No Hit
AATCTGTCTCTTATACACATCTGACGCCTCGTTTGTCGTATGCCGTCTTCT	1353	0.24725425339449209	No Hit
AATGATACGGCTGTCTCTTATACACATCTGACGCCTCGTTTGTCGTATGCC	1280	0.2339138539134884	No Hit
CAGCACGGGAATGATACTGTCTCTTATACACATCTGACGCCTCGTTTGTCG	1261	0.23044169514446008	No Hit
AAAAACTGTCTCTTATACACATCTGACGCCTCGTTTGTCGTATGCCGTCTT	1243	0.22715228157380163	No Hit
AATGATACCTGTCTCTTATACACATCTGACGCCTCGTTTGTCGTATGCCGT	1220	0.22294914201129368	No Hit
CAGCACGGGAATGATACCTGTCTCTTATACACATCTGACGCCTCGTTTGTC	1071	0.19572010745417665	No Hit
CCTGTCTCTTATACACATCTGACGCCTCGTTTGTCGTATGCCGTCTTCTGC	929	0.16977028928564902	TruSeq Adapter, Index 20 (95% over 21bp)
TGCAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGG	866	0.15825734178834452	No Hit
CAGCACGGGAATGATACGGCTGTCTCTTATACACATCTGACGCCTCGTTTG	815	0.14893733667147896	No Hit
AAAAAACTGTCTCTTATACACATCTGACGCCTCGTTTGTCGTATGCCGTCT	695	0.12700791286708943	No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	673	0.12298751850295132	No Hit
CAGCACGGGAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCC	643	0.11750516255185393	No Hit
CAGCACGGGAACTGTCTCTTATACACATCTGACGCCTCGTTTGTCGTATGC	593	0.1083679026333583	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	569	0.10398201787248039	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	3.6549039673982567E-4	0.0	0.0	0.660441146908865	0.0
2	3.6549039673982567E-4	0.0	0.0	1.0082052594068092	0.0
3	3.6549039673982567E-4	0.0	0.0	1.3704062425759762	0.0
4	3.6549039673982567E-4	0.0	0.0	3.068657371027576	0.0
5	3.6549039673982567E-4	0.0	0.0	3.3829791122238264	0.0
6	3.6549039673982567E-4	0.0	0.0	4.938323495550154	0.0
7	3.6549039673982567E-4	0.0	0.0	6.161071617843241	0.0
8	3.6549039673982567E-4	0.0	0.0	7.257908298459458	0.0
9	3.6549039673982567E-4	0.0	0.0	8.517388205624897	0.0
10	3.6549039673982567E-4	0.0	0.0	10.556276383838014	0.0
11	3.6549039673982567E-4	0.0	0.0	12.141956470093747	0.0
12	3.6549039673982567E-4	0.0	0.0	13.116902103397234	0.0
13	3.6549039673982567E-4	0.0	0.0	13.54324665119424	0.0
14	3.6549039673982567E-4	0.0	0.0	13.932676668920523	0.0
15	3.6549039673982567E-4	0.0	0.0	14.259973319201038	0.0
16	3.6549039673982567E-4	0.0	0.0	14.914932110158805	0.0
17	3.6549039673982567E-4	0.0	0.0	15.926061292739533	0.0
18	3.6549039673982567E-4	0.0	0.0	17.31675225233457	0.0
19	3.6549039673982567E-4	0.0	0.0	17.752965040843552	0.0
20	3.6549039673982567E-4	0.0	0.0	18.43003600080408	0.0
21	3.6549039673982567E-4	0.0	0.0	18.917417444856635	0.0
22	3.6549039673982567E-4	0.0	0.0	19.416129091208127	0.0
23	3.6549039673982567E-4	0.0	0.0	19.93530819977705	0.0
24	3.6549039673982567E-4	0.0	0.0	20.282524076679884	0.0
25	3.6549039673982567E-4	0.0	0.0	20.5836881635935	0.0
26	3.6549039673982567E-4	0.0	0.0	20.883207543721788	0.0
27	3.6549039673982567E-4	0.0	0.0	21.38612232963579	0.0
28	3.6549039673982567E-4	0.0	0.0	21.793461376802323	0.0
29	3.6549039673982567E-4	0.0	0.0	22.125692147438826	0.0
30	3.6549039673982567E-4	0.0	0.0	22.790519179108568	0.0
31	3.6549039673982567E-4	0.0	0.0	23.1019169971309	0.0
32	3.6549039673982567E-4	0.0	0.0	23.442736792090788	0.0
33	3.6549039673982567E-4	0.0	0.0	23.767292264395753	0.0
34	3.6549039673982567E-4	0.0	0.0	24.06900458690448	0.0
35	3.6549039673982567E-4	0.0	0.0	24.392646333217595	0.0
36	3.6549039673982567E-4	0.0	0.0	24.678825313864877	0.0
37	3.6549039673982567E-4	0.0	0.0	24.973593318835547	0.0
38	3.6549039673982567E-4	0.0	0.0	25.28407741086603	0.0
39	3.6549039673982567E-4	0.0	0.0	25.623069753842216	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCACGCG	20	7.030042E-4	45.000004	1
AAATCGT	20	7.030042E-4	45.000004	43
CGAACAT	40	6.8030204E-9	45.000004	17
GATCGTA	20	7.030042E-4	45.000004	21
CACGGTA	20	7.030042E-4	45.000004	4
ACGTTGC	20	7.030042E-4	45.000004	11
ACGCATA	20	7.030042E-4	45.000004	20
TTAGCGT	20	7.030042E-4	45.000004	19
CGCATAG	20	7.030042E-4	45.000004	1
CGGCAAA	20	7.030042E-4	45.000004	11
GAATCGA	20	7.030042E-4	45.000004	24
CGTCTGA	20	7.030042E-4	45.000004	28
CGACACG	20	7.030042E-4	45.000004	1
GCGTTGA	20	7.030042E-4	45.000004	13
CCTCGCG	20	7.030042E-4	45.000004	36
ATCGTAC	20	7.030042E-4	45.000004	29
ATCGTAA	20	7.030042E-4	45.000004	17
ATCCGAG	20	7.030042E-4	45.000004	1
ACGATTC	20	7.030042E-4	45.000004	14
CCCGTTA	40	6.8030204E-9	45.000004	14
>>END_MODULE