SRR3548393_1.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR3548393_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	553095
Sequences flagged as poor quality	0
Sequence length	51
%GC	49

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
CTGTCTCTTATACACATCTGACGCCTCGTTTGTCGTATGCCGTCTTCTGCT	3470	0.6273786600855188	TruSeq Adapter, Index 27 (95% over 23bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2930	0.5297462461240836	No Hit
AAACTGTCTCTTATACACATCTGACGCCTCGTTTGTCGTATGCCGTCTTCT	1493	0.2699355445267088	No Hit
CAGCACGGGAATGATACTGTCTCTTATACACATCTGACGCCTCGTTTGTCG	1489	0.2692123414603278	No Hit
AATGATACGGCTGTCTCTTATACACATCTGACGCCTCGTTTGTCGTATGCC	1484	0.26830833762735157	No Hit
AATCTGTCTCTTATACACATCTGACGCCTCGTTTGTCGTATGCCGTCTTCT	1449	0.26198031079651773	No Hit
AATGATACCTGTCTCTTATACACATCTGACGCCTCGTTTGTCGTATGCCGT	1379	0.24932425713485024	No Hit
AAAAACTGTCTCTTATACACATCTGACGCCTCGTTTGTCGTATGCCGTCTT	1321	0.23883781267232573	No Hit
CAGCACGGGAATGATACCTGTCTCTTATACACATCTGACGCCTCGTTTGTC	1226	0.22166173984577695	No Hit
CCTGTCTCTTATACACATCTGACGCCTCGTTTGTCGTATGCCGTCTTCTGC	1003	0.1813431688950361	TruSeq Adapter, Index 20 (95% over 21bp)
CAGCACGGGAATGATACGGCTGTCTCTTATACACATCTGACGCCTCGTTTG	913	0.1650710999014636	No Hit
TGCAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGG	807	0.14590621864236705	No Hit
AAAAAACTGTCTCTTATACACATCTGACGCCTCGTTTGTCGTATGCCGTCT	750	0.13560057494643776	No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	689	0.12457172818412751	No Hit
CAGCACGGGAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCC	683	0.12348692358455601	No Hit
CAGCACGGGAACTGTCTCTTATACACATCTGACGCCTCGTTTGTCGTATGC	677	0.12240211898498449	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	561	0.10142923005993547	No Hit

Adapter graph

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CCTTACG	35	1.2103919E-7	45.000004	1
TTATGCG	35	1.2103919E-7	45.000004	1
TATTACG	35	1.2103919E-7	45.000004	30
TCACGCG	40	6.8048394E-9	45.0	1
CTTCGAC	20	7.030108E-4	45.0	23
AATCCGT	20	7.030108E-4	45.0	44
CCTTACT	20	7.030108E-4	45.0	27
GTACCGA	25	3.888172E-5	45.0	14
CGAACTT	20	7.030108E-4	45.0	5
TACCCGG	50	2.1827873E-11	45.0	2
CGAACGT	25	3.888172E-5	45.0	10
CGACGTT	25	3.888172E-5	45.0	24
ACGTTGT	20	7.030108E-4	45.0	36
ACCGCTT	20	7.030108E-4	45.0	10
GACCGAA	25	3.888172E-5	45.0	9
GAATCGT	25	3.888172E-5	45.0	15
TCGGGAT	25	3.888172E-5	45.0	5
CGTCTTG	25	3.888172E-5	45.0	22
GAATACG	20	7.030108E-4	45.0	1
CCGTCGA	20	7.030108E-4	45.0	15