##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3548393_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	553095
Sequences flagged as poor quality	0
Sequence length	51
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.68113796002495	31.0	31.0	34.0	30.0	34.0
2	31.878113163199814	33.0	31.0	34.0	30.0	34.0
3	32.03520371726376	33.0	31.0	34.0	30.0	34.0
4	35.722018821359804	37.0	35.0	37.0	33.0	37.0
5	35.49400374257587	37.0	35.0	37.0	33.0	37.0
6	35.52189949285385	37.0	35.0	37.0	33.0	37.0
7	35.58736202641499	37.0	35.0	37.0	35.0	37.0
8	35.624814905215196	37.0	35.0	37.0	35.0	37.0
9	37.206051401657945	39.0	37.0	39.0	34.0	39.0
10	36.85247019047361	39.0	37.0	39.0	32.0	39.0
11	36.87315018215677	39.0	37.0	39.0	33.0	39.0
12	36.83472459523228	39.0	35.0	39.0	33.0	39.0
13	36.77612706677876	39.0	35.0	39.0	33.0	39.0
14	37.88657644708414	40.0	37.0	41.0	33.0	41.0
15	37.92697095435685	40.0	37.0	41.0	33.0	41.0
16	37.82503367414278	40.0	37.0	41.0	33.0	41.0
17	37.75564776394652	39.0	36.0	41.0	33.0	41.0
18	37.654659687757075	39.0	36.0	41.0	33.0	41.0
19	37.65164935499327	39.0	36.0	41.0	33.0	41.0
20	37.47608096258328	39.0	36.0	41.0	32.0	41.0
21	37.42037805440295	39.0	36.0	41.0	32.0	41.0
22	37.37549064808035	39.0	36.0	41.0	32.0	41.0
23	37.2193131378877	39.0	35.0	41.0	32.0	41.0
24	37.12228821450203	39.0	35.0	41.0	31.0	41.0
25	37.045209231687146	39.0	35.0	41.0	31.0	41.0
26	37.07102938916461	39.0	35.0	41.0	31.0	41.0
27	36.99922617271897	39.0	35.0	41.0	31.0	41.0
28	36.79200679810882	39.0	35.0	41.0	31.0	41.0
29	36.5696995995263	39.0	35.0	40.0	30.0	41.0
30	36.64161672045489	39.0	35.0	40.0	30.0	41.0
31	36.533732903027506	39.0	35.0	40.0	30.0	41.0
32	36.42171417206809	39.0	35.0	40.0	30.0	41.0
33	36.348925591444505	39.0	35.0	40.0	30.0	41.0
34	36.25556911561305	39.0	35.0	40.0	30.0	41.0
35	36.1597537493559	39.0	35.0	40.0	30.0	41.0
36	36.07708621484555	39.0	35.0	40.0	30.0	41.0
37	35.94698740722661	38.0	35.0	40.0	29.0	41.0
38	35.83497410029018	38.0	35.0	40.0	29.0	41.0
39	35.71662734249993	38.0	35.0	40.0	28.0	41.0
40	35.58132870483371	38.0	35.0	40.0	28.0	41.0
41	35.51564197832199	38.0	35.0	40.0	27.0	41.0
42	35.37618673103174	38.0	34.0	40.0	27.0	41.0
43	35.303962248799934	38.0	34.0	40.0	26.0	41.0
44	35.208148690550445	38.0	34.0	40.0	26.0	41.0
45	35.00033086540287	38.0	34.0	40.0	26.0	41.0
46	34.857252370750054	37.0	34.0	40.0	25.0	41.0
47	34.706131857999075	37.0	34.0	40.0	24.0	41.0
48	34.59159638036865	37.0	34.0	40.0	24.0	41.0
49	34.375747385168914	37.0	33.0	40.0	24.0	41.0
50	34.30570155217458	37.0	33.0	40.0	24.0	41.0
51	32.995317260145185	35.0	31.0	39.0	20.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	3.0
10	8.0
11	9.0
12	10.0
13	13.0
14	14.0
15	37.0
16	75.0
17	195.0
18	389.0
19	724.0
20	1383.0
21	2226.0
22	2975.0
23	3733.0
24	4254.0
25	4860.0
26	5524.0
27	6181.0
28	7333.0
29	9048.0
30	11558.0
31	14375.0
32	18147.0
33	24745.0
34	37361.0
35	46233.0
36	52112.0
37	74059.0
38	108521.0
39	116959.0
40	31.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	20.037064157152027	32.24382791383035	20.48400365217549	27.235104276842133
2	23.57280394868874	34.43657961109755	18.006490747520772	23.98412569269294
3	22.285502490530558	31.967925944006005	19.605492727289164	26.141078838174277
4	20.81703866424394	31.81171408166771	17.624097126171815	29.747150127916544
5	17.36844484220613	36.61938726620201	17.703287861940534	28.308880029651323
6	19.597718294325567	36.92674856941394	19.053327186107268	24.422205950153227
7	81.48328948914744	7.648053227745685	6.109981106319891	4.75867617678699
8	82.51674667100588	6.154819696435513	5.0589862501017	6.269447382456901
9	75.86291685876748	9.73847169111997	9.347038031441253	5.051573418671295
10	32.33820591399307	35.58972690044206	13.634005008181235	18.43806217738363
11	25.506468147424943	32.58427575732921	23.780905631039875	18.12835046420597
12	26.549146168379757	23.70948932823475	30.035346549869374	19.706017953516124
13	27.099322901129103	25.176868349921804	26.27595621005433	21.447852538894765
14	20.194722425623084	29.645178495556824	28.46816550502174	21.69193357379835
15	19.29704661947767	29.57032697818639	30.44449868467442	20.688127717661523
16	20.869290085789963	29.565264556721722	26.16241332863251	23.4030320288558
17	19.295600213344905	27.21087697411837	27.195508908957773	26.298013903578948
18	23.445881810538875	26.415172800332677	25.960458872345622	24.178486516782833
19	24.439201222213182	30.893607788897025	24.132743922834234	20.534447066055563
20	23.449678626637375	28.14923295274772	24.688706280114626	23.712382140500278
21	23.76354875744673	29.69327149947116	24.66267096972491	21.8805087733572
22	20.23413699274085	29.51210913134272	24.425098762418752	25.828655113497685
23	22.65053923828637	27.85145409016534	23.70424610600349	25.793760565544797
24	20.512208571764347	28.249034975908298	24.318064708594363	26.920691743732995
25	21.325992822209567	29.115793850965925	23.937840696444553	25.62037263037995
26	21.703866424393638	28.24433415597682	24.609515544345907	25.442283875283632
27	19.669134597130693	27.475388495647223	24.448964463609325	28.406512443612762
28	22.19220929496741	27.079796418336816	26.29150507598152	24.436489210714253
29	22.461964038727526	29.17997812310724	24.10923982317685	24.248818014988384
30	22.598830219040128	26.24522007973314	24.721973621168154	26.43397608005858
31	23.869859608204738	26.81944331443965	23.926269447382456	25.38442762997315
32	22.833871215613954	25.70806100217865	25.166743506992468	26.29132427521493
33	22.99695350708287	25.055912637069582	26.54769976224699	25.399434093600558
34	21.224744392916225	24.05445719089849	28.749491497843948	25.971306918341337
35	22.859002522170695	23.832976251819307	27.91057594084199	25.397445285168008
36	22.538804364530506	25.65815999059836	28.612263715998154	23.190771928872977
37	24.124788689104044	25.490557679964564	26.04977445104367	24.33487917988772
38	24.851065368517162	24.292210198971244	28.757265930807545	22.099458501704046
39	23.967130420632984	22.21969101148989	28.891600900387814	24.921577667489313
40	23.78217123640604	23.075963442084994	28.60105406846925	24.540811253039713
41	21.724658512552093	21.805657255986766	30.840271562751425	25.629412668709715
42	24.488198229960496	24.059158010829965	25.824677496632585	25.62796626257695
43	21.84507182310453	23.439192182174853	28.189009121398673	26.526726873321945
44	24.64947251376346	20.747068767571577	26.34484130212712	28.258617416537845
45	25.976911742105784	21.859355083665555	27.98868187201114	24.175051302217522
46	21.037253997956952	23.10706117393938	31.477413464233088	24.37827136387058
47	22.660302479682514	24.080492501288205	28.05557815565138	25.2036268633779
48	22.56990209638489	22.506260226543358	31.24653088529095	23.677306791780797
49	23.770238385810753	21.78793878086043	29.616792775201368	24.825030058127446
50	22.12947142895886	21.408980374076787	27.80155307858505	28.6599951183793
51	22.186062068903173	20.98409857257795	31.125032770138944	25.704806588379935
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	3548.0
1	2901.0
2	2254.0
3	1605.0
4	956.0
5	963.5
6	971.0
7	1069.5
8	1168.0
9	1269.0
10	1370.0
11	1507.5
12	1645.0
13	1848.0
14	2051.0
15	1967.0
16	1883.0
17	1809.0
18	1735.0
19	1745.0
20	1755.0
21	1679.0
22	1603.0
23	1898.5
24	2194.0
25	2766.0
26	3705.0
27	4072.0
28	4700.0
29	5328.0
30	6171.5
31	7015.0
32	7697.0
33	8379.0
34	9047.5
35	9716.0
36	10987.5
37	12259.0
38	14176.0
39	16093.0
40	16578.5
41	17064.0
42	20496.5
43	23929.0
44	29549.0
45	35169.0
46	39847.0
47	44525.0
48	50238.0
49	55951.0
50	54036.0
51	52121.0
52	47855.0
53	43589.0
54	40289.5
55	36990.0
56	33488.5
57	29987.0
58	28042.5
59	26098.0
60	25090.0
61	24082.0
62	22146.5
63	20211.0
64	17588.5
65	14966.0
66	12786.5
67	10607.0
68	9277.5
69	7948.0
70	6735.0
71	5522.0
72	5347.5
73	5173.0
74	4288.5
75	3119.0
76	2834.0
77	2132.0
78	1430.0
79	1222.5
80	1015.0
81	768.5
82	522.0
83	383.0
84	244.0
85	198.5
86	153.0
87	151.0
88	149.0
89	106.5
90	64.0
91	39.0
92	14.0
93	7.0
94	0.0
95	0.5
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	553095.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	29.12734924154245
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.51886523351055	22.287917112229827
2	9.930551744482319	5.785012976454902
3	3.8754586628247183	3.3864551382977
4	1.9049880732949411	2.2194901164733927
5	1.1647425194237984	1.6962931069865503
6	0.7768520117736024	1.3576583913554738
7	0.5025751412159192	1.0247077160819513
8	0.39126019770992715	0.9117097938409594
9	0.31956431747606007	0.8377255332234314
>10	3.2517667622340958	22.4149339289559
>50	1.0365901386406187	21.319547958075294
>100	0.3161631164268554	12.69567132871291
>500	0.004373798053335944	0.9245392693291319
>1k	0.006248282933337063	3.1383376299825883
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTGTCTCTTATACACATCTGACGCCTCGTTTGTCGTATGCCGTCTTCTGCT	3470	0.6273786600855188	TruSeq Adapter, Index 27 (95% over 23bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2930	0.5297462461240836	No Hit
AAACTGTCTCTTATACACATCTGACGCCTCGTTTGTCGTATGCCGTCTTCT	1493	0.2699355445267088	No Hit
CAGCACGGGAATGATACTGTCTCTTATACACATCTGACGCCTCGTTTGTCG	1489	0.2692123414603278	No Hit
AATGATACGGCTGTCTCTTATACACATCTGACGCCTCGTTTGTCGTATGCC	1484	0.26830833762735157	No Hit
AATCTGTCTCTTATACACATCTGACGCCTCGTTTGTCGTATGCCGTCTTCT	1449	0.26198031079651773	No Hit
AATGATACCTGTCTCTTATACACATCTGACGCCTCGTTTGTCGTATGCCGT	1379	0.24932425713485024	No Hit
AAAAACTGTCTCTTATACACATCTGACGCCTCGTTTGTCGTATGCCGTCTT	1321	0.23883781267232573	No Hit
CAGCACGGGAATGATACCTGTCTCTTATACACATCTGACGCCTCGTTTGTC	1226	0.22166173984577695	No Hit
CCTGTCTCTTATACACATCTGACGCCTCGTTTGTCGTATGCCGTCTTCTGC	1003	0.1813431688950361	TruSeq Adapter, Index 20 (95% over 21bp)
CAGCACGGGAATGATACGGCTGTCTCTTATACACATCTGACGCCTCGTTTG	913	0.1650710999014636	No Hit
TGCAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGG	807	0.14590621864236705	No Hit
AAAAAACTGTCTCTTATACACATCTGACGCCTCGTTTGTCGTATGCCGTCT	750	0.13560057494643776	No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	689	0.12457172818412751	No Hit
CAGCACGGGAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCC	683	0.12348692358455601	No Hit
CAGCACGGGAACTGTCTCTTATACACATCTGACGCCTCGTTTGTCGTATGC	677	0.12240211898498449	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	561	0.10142923005993547	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.8080076659525036E-4	0.0	0.0	0.7143438288178342	0.0
2	1.8080076659525036E-4	0.0	0.0	1.0842621972717164	0.0
3	1.8080076659525036E-4	0.0	0.0	1.4787694699825527	0.0
4	1.8080076659525036E-4	0.0	0.0	3.3120892432583915	0.0
5	1.8080076659525036E-4	0.0	0.0	3.6312025962990084	0.0
6	1.8080076659525036E-4	0.0	0.0	5.258228694889667	0.0
7	1.8080076659525036E-4	0.0	0.0	6.53178929478661	0.0
8	1.8080076659525036E-4	0.0	0.0	7.634493170251042	0.0
9	1.8080076659525036E-4	0.0	0.0	8.992668528914562	0.0
10	1.8080076659525036E-4	0.0	0.0	11.076397363924823	0.0
11	1.8080076659525036E-4	0.0	0.0	12.727650765239245	0.0
12	1.8080076659525036E-4	0.0	0.0	13.732541425975645	0.0
13	1.8080076659525036E-4	0.0	0.0	14.169717679602961	0.0
14	1.8080076659525036E-4	0.0	0.0	14.576881005975466	0.0
15	1.8080076659525036E-4	0.0	0.0	14.92763449317025	0.0
16	1.8080076659525036E-4	0.0	0.0	15.590992505808225	0.0
17	1.8080076659525036E-4	0.0	0.0	16.688814760574584	0.0
18	1.8080076659525036E-4	0.0	0.0	18.17174264818883	0.0
19	1.8080076659525036E-4	0.0	0.0	18.603133277285096	0.0
20	1.8080076659525036E-4	0.0	0.0	19.32163552373462	0.0
21	1.8080076659525036E-4	0.0	0.0	19.823538451803035	0.0
22	1.8080076659525036E-4	0.0	0.0	20.35057268642819	0.0
23	1.8080076659525036E-4	0.0	0.0	20.8908053770148	0.0
24	1.8080076659525036E-4	0.0	0.0	21.240474059610012	0.0
25	1.8080076659525036E-4	0.0	0.0	21.55235538198682	0.0
26	1.8080076659525036E-4	0.0	0.0	21.843987018504958	0.0
27	1.8080076659525036E-4	0.0	0.0	22.351133168804637	0.0
28	1.8080076659525036E-4	0.0	0.0	22.77691897413645	0.0
29	1.8080076659525036E-4	0.0	0.0	23.118994024534665	0.0
30	1.8080076659525036E-4	0.0	0.0	23.780182427973493	0.0
31	1.8080076659525036E-4	0.0	0.0	24.085374121986277	0.0
32	1.8080076659525036E-4	0.0	0.0	24.44155163217892	0.0
33	1.8080076659525036E-4	0.0	0.0	24.770247425849085	0.0
34	1.8080076659525036E-4	0.0	0.0	25.079778338260155	0.0
35	1.8080076659525036E-4	0.0	0.0	25.40883573346351	0.0
36	1.8080076659525036E-4	0.0	0.0	25.72216346197308	0.0
37	1.8080076659525036E-4	0.0	0.0	26.02554714831991	0.0
38	1.8080076659525036E-4	0.0	0.0	26.35894376192155	0.0
39	1.8080076659525036E-4	0.0	0.0	26.684204341026405	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CCTTACG	35	1.2103919E-7	45.000004	1
TTATGCG	35	1.2103919E-7	45.000004	1
TATTACG	35	1.2103919E-7	45.000004	30
TCACGCG	40	6.8048394E-9	45.0	1
CTTCGAC	20	7.030108E-4	45.0	23
AATCCGT	20	7.030108E-4	45.0	44
CCTTACT	20	7.030108E-4	45.0	27
GTACCGA	25	3.888172E-5	45.0	14
CGAACTT	20	7.030108E-4	45.0	5
TACCCGG	50	2.1827873E-11	45.0	2
CGAACGT	25	3.888172E-5	45.0	10
CGACGTT	25	3.888172E-5	45.0	24
ACGTTGT	20	7.030108E-4	45.0	36
ACCGCTT	20	7.030108E-4	45.0	10
GACCGAA	25	3.888172E-5	45.0	9
GAATCGT	25	3.888172E-5	45.0	15
TCGGGAT	25	3.888172E-5	45.0	5
CGTCTTG	25	3.888172E-5	45.0	22
GAATACG	20	7.030108E-4	45.0	1
CCGTCGA	20	7.030108E-4	45.0	15
>>END_MODULE