##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3548392_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	331104
Sequences flagged as poor quality	0
Sequence length	51
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.860729559292547	33.0	31.0	34.0	30.0	34.0
2	32.04274789794143	33.0	31.0	34.0	30.0	34.0
3	32.21128708804484	34.0	31.0	34.0	30.0	34.0
4	35.85892046003673	37.0	35.0	37.0	35.0	37.0
5	35.63312131535711	37.0	35.0	37.0	33.0	37.0
6	34.98901553590413	37.0	35.0	37.0	33.0	37.0
7	35.3947037788731	37.0	35.0	37.0	33.0	37.0
8	35.762056634773366	37.0	35.0	37.0	35.0	37.0
9	37.3670900985793	39.0	37.0	39.0	35.0	39.0
10	36.98622789214265	39.0	37.0	39.0	33.0	39.0
11	37.02970667826423	39.0	37.0	39.0	33.0	39.0
12	36.961809582487675	39.0	37.0	39.0	33.0	39.0
13	36.90170460036725	39.0	37.0	39.0	33.0	39.0
14	38.08947641828549	40.0	37.0	41.0	33.0	41.0
15	38.166645525273026	40.0	37.0	41.0	33.0	41.0
16	38.050337658258435	40.0	37.0	41.0	33.0	41.0
17	38.019371556973034	40.0	37.0	41.0	33.0	41.0
18	37.93184014690248	40.0	37.0	41.0	33.0	41.0
19	37.95206642021842	40.0	37.0	41.0	33.0	41.0
20	37.86652532134919	40.0	37.0	41.0	33.0	41.0
21	37.854598555136754	40.0	37.0	41.0	33.0	41.0
22	37.80574683483135	40.0	36.0	41.0	33.0	41.0
23	37.70630073934474	40.0	36.0	41.0	33.0	41.0
24	37.59998369092491	39.0	36.0	41.0	33.0	41.0
25	37.56911121581135	39.0	36.0	41.0	32.0	41.0
26	37.580838648883734	39.0	36.0	41.0	33.0	41.0
27	37.497771093070455	39.0	36.0	41.0	33.0	41.0
28	37.32437542282787	39.0	36.0	41.0	32.0	41.0
29	36.97647566927612	39.0	35.0	41.0	31.0	41.0
30	37.06305571663284	39.0	35.0	41.0	31.0	41.0
31	37.00998779839567	39.0	35.0	41.0	31.0	41.0
32	37.013908016816465	39.0	35.0	41.0	31.0	41.0
33	36.79990274958925	39.0	35.0	41.0	31.0	41.0
34	36.78375374504687	39.0	35.0	41.0	31.0	41.0
35	36.72296921813086	39.0	35.0	41.0	31.0	41.0
36	36.65195829709094	39.0	35.0	41.0	31.0	41.0
37	36.61146346767179	39.0	35.0	41.0	31.0	41.0
38	36.412045762056636	39.0	35.0	40.0	30.0	41.0
39	36.37163247801295	39.0	35.0	40.0	30.0	41.0
40	36.305586764279504	39.0	35.0	40.0	30.0	41.0
41	36.22106951290229	39.0	35.0	40.0	30.0	41.0
42	36.118536773944136	38.0	35.0	40.0	30.0	41.0
43	35.978263627138304	38.0	35.0	40.0	29.0	41.0
44	35.92421716439548	38.0	35.0	40.0	29.0	41.0
45	35.75660215521407	38.0	35.0	40.0	29.0	41.0
46	35.605093867787765	38.0	34.0	40.0	28.0	41.0
47	35.48287849135015	38.0	34.0	40.0	28.0	41.0
48	35.36025236783609	38.0	34.0	40.0	28.0	41.0
49	35.17901626075191	38.0	34.0	40.0	27.0	41.0
50	35.01863160819561	37.0	34.0	40.0	26.0	41.0
51	33.79216499951677	36.0	32.0	39.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	2.0
11	1.0
12	6.0
13	8.0
14	10.0
15	25.0
16	38.0
17	64.0
18	152.0
19	301.0
20	534.0
21	782.0
22	1128.0
23	1480.0
24	1841.0
25	2103.0
26	2448.0
27	2987.0
28	3629.0
29	4645.0
30	6039.0
31	7737.0
32	10106.0
33	13816.0
34	21311.0
35	26730.0
36	30340.0
37	43802.0
38	66741.0
39	82269.0
40	28.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	21.60710834058181	32.5402290518991	19.76569295447956	26.086969653039528
2	24.37512080796366	30.578005702135886	19.274306562288587	25.772566927611866
3	20.808567700782834	32.286230308301924	20.25828742630714	26.6469145646081
4	21.336196482072097	31.825347926935343	19.38152363003769	27.45693196095487
5	18.00974920266744	33.4985382236397	17.222987339325407	31.26872523436745
6	19.6207233980864	38.68150188460424	19.3135691504784	22.384205566830964
7	85.32334251473857	5.763445926355466	4.932891176186334	3.9803203827196287
8	86.33631729003575	5.020476949840534	4.075456654102638	4.567749106021069
9	78.83293466705325	9.017710447472698	7.464120034792694	4.685234850681357
10	33.69696530395284	36.096815502077895	12.749468444959891	17.456750749009377
11	25.6448125060404	31.612423890982893	24.783753745046873	17.959009857929832
12	25.797634580071517	23.333756161206146	32.05699719725524	18.81161206146709
13	27.153401952256694	24.246762346573885	27.289008891466125	21.310826809703297
14	21.27881269933314	30.68975306852228	27.47897941432299	20.55245481782159
15	19.82247269740021	29.293213008601526	28.95797090944235	21.92634338455591
16	21.67929109886924	29.394691698076734	26.658693341065042	22.267323861988984
17	21.116628008118298	26.162172610418477	26.736916497535518	25.984282883927705
18	24.633951870107275	26.30412196772011	25.62397313230888	23.437953029863728
19	24.173069488740698	30.433036145742726	24.98882526336136	20.405069102155213
20	24.568111529912052	27.628177249444285	24.1857543249251	23.617956895718567
21	26.300799748719434	27.227698849908187	25.346114815888665	21.125386585483717
22	22.2881632357205	28.289298830578915	24.1229341838214	25.29960374987919
23	21.72972842369769	28.670749975838405	24.121726104184788	25.477795496279114
24	20.169795592925485	28.705784285300084	24.688919493573017	26.435500628201414
25	22.178227988789022	28.86706291678747	24.22864115202474	24.726067942398764
26	23.720643664830384	27.55418237170194	23.132610901710642	25.59256306175703
27	21.2549531265101	26.188146322605586	25.522494442833672	27.03440610805064
28	23.7472214168358	27.329177539383398	26.752017492993136	22.17158355078767
29	22.761428433362326	30.704854063979898	23.89883541123031	22.634882091427468
30	25.36725620952933	26.64600850488064	26.144051415869335	21.84268386972069
31	24.74690731613028	28.954950710350825	22.773509229728422	23.524632743790473
32	24.158270513192228	29.190526239489706	23.651481105634485	22.99972214168358
33	23.790712283753745	27.024741470957764	26.356371412003483	22.82817483328501
34	23.69376389291582	27.716971102735094	24.86469508069972	23.724569923649366
35	24.20327147965594	28.483799652073067	26.33130375954383	20.98162510872717
36	24.002126220160434	28.48832995071035	26.23224122934184	21.27730259978738
37	24.44005508843143	29.287172610418477	23.24617038755195	23.026601913598142
38	24.366664250507394	27.641768145356142	25.198729100222288	22.79283850391418
39	25.545145936020102	24.672308398569633	25.018121194549142	24.76442447086112
40	24.068268580264814	25.335242099159178	26.043780806030735	24.55270851454528
41	22.138965400599208	25.164600850488068	27.183301923262782	25.51313182564995
42	25.329201700976128	26.52580458103798	24.769256789407557	23.37573692857833
43	22.411689378563835	26.297175509809605	25.890958731999614	25.40017637962694
44	23.889774813955736	23.590171064076543	26.10086256886054	26.41919155310718
45	24.463612641345318	24.465726780709385	26.71215086498502	24.358509712960277
46	22.391756064559775	25.819984053348797	28.494068328984245	23.29419155310718
47	23.437046970136272	25.938073837827393	26.177575625785256	24.447303566251087
48	22.447629747752973	25.145573596211463	26.900611288296123	25.50618536773944
49	23.84537788731033	25.145875616120616	27.170919106987533	23.83782738958152
50	22.746327437904707	24.03444235043974	27.275115975645114	25.944114236010442
51	23.73755677974292	23.260667343191262	28.031977867981055	24.96979800908476
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1778.0
1	1425.0
2	1072.0
3	741.0
4	410.0
5	372.0
6	334.0
7	356.5
8	379.0
9	429.0
10	479.0
11	496.5
12	514.0
13	657.5
14	801.0
15	713.5
16	626.0
17	674.0
18	722.0
19	762.0
20	802.0
21	901.5
22	1001.0
23	1325.5
24	1650.0
25	1713.5
26	1998.0
27	2219.0
28	2993.0
29	3767.0
30	4080.5
31	4394.0
32	5390.5
33	6387.0
34	6961.5
35	7536.0
36	8303.0
37	9070.0
38	9274.0
39	9478.0
40	11312.5
41	13147.0
42	14177.0
43	15207.0
44	18240.0
45	21273.0
46	24193.0
47	27113.0
48	29306.0
49	31499.0
50	29924.0
51	28349.0
52	26646.0
53	24943.0
54	22896.5
55	20850.0
56	18965.0
57	17080.0
58	17113.5
59	17147.0
60	16659.0
61	16171.0
62	14400.0
63	12629.0
64	10862.0
65	9095.0
66	7730.5
67	6366.0
68	5616.0
69	4866.0
70	4262.5
71	3659.0
72	3236.5
73	2814.0
74	2091.5
75	1213.0
76	1057.0
77	836.5
78	616.0
79	419.0
80	222.0
81	186.0
82	150.0
83	141.5
84	133.0
85	94.0
86	55.0
87	60.0
88	65.0
89	45.5
90	26.0
91	16.0
92	6.0
93	3.0
94	0.0
95	0.0
96	0.0
97	0.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	331104.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	30.49713726173667
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.35883321295772	23.897200921651017
2	8.735955948104607	5.3284329532366215
3	2.982864292123137	2.7290646525003686
4	1.4119076912776818	1.722365706471887
5	0.8189460132400923	1.2487754487867553
6	0.5889611656078899	1.077697770562574
7	0.44597059381065	0.9520578489899121
8	0.3819748135286506	0.9319310654967621
9	0.3239786376524746	0.8892378884122167
>10	4.86467923512122	36.249251905922804
>50	1.0009340008954455	19.839519391056758
>100	0.08299452754677519	4.217171042130467
>500	0.0	0.0
>1k	0.0019998681336572334	0.9172934047818673
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTGTCTCTTATACACATCTGACGCTAGGATCGTCGTATGCCGTCTTCTGCT	1530	0.46209046100318935	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1478	0.446385425727264	No Hit
AAACTGTCTCTTATACACATCTGACGCTAGGATCGTCGTATGCCGTCTTCT	499	0.15070793466705326	No Hit
AATCTGTCTCTTATACACATCTGACGCTAGGATCGTCGTATGCCGTCTTCT	456	0.13772107857349958	No Hit
AATGATACGGCTGTCTCTTATACACATCTGACGCTAGGATCGTCGTATGCC	438	0.13228472020875615	No Hit
AATGATACCTGTCTCTTATACACATCTGACGCTAGGATCGTCGTATGCCGT	425	0.1283584613897748	No Hit
AGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT	390	0.11778776456944041	No Hit
CCTGTCTCTTATACACATCTGACGCTAGGATCGTCGTATGCCGTCTTCTGC	366	0.11053928674978256	No Hit
TGCAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGG	356	0.10751908765825842	No Hit
AAAAACTGTCTCTTATACACATCTGACGCTAGGATCGTCGTATGCCGTCTT	347	0.10480090847588674	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.5212863631970619	0.0
2	0.0	0.0	0.0	0.7719628877935634	0.0
3	0.0	0.0	0.0	1.0289818304822653	0.0
4	0.0	0.0	0.0	2.227698849908186	0.0
5	0.0	0.0	0.0	2.4786773944138396	0.0
6	0.0	0.0	0.0	3.567761186817435	0.0
7	0.0	0.0	0.0	4.351502851067942	0.0
8	0.0	0.0	0.0	5.131318256499468	0.0
9	0.0	0.0	0.0	6.036169904320093	0.0
10	0.0	0.0	0.0	7.481033149705229	0.0
11	0.0	0.0	0.0	8.632937083212525	0.0
12	0.0	0.0	0.0	9.304629361167487	0.0
13	0.0	0.0	0.0	9.695443123610708	0.0
14	0.0	0.0	0.0	10.043370058954286	0.0
15	0.0	0.0	0.0	10.284985986276215	0.0
16	3.0201990915241134E-4	0.0	0.0	10.83164202184208	0.0
17	3.0201990915241134E-4	0.0	0.0	11.430547501691311	0.0
18	3.0201990915241134E-4	0.0	0.0	12.454697013627138	0.0
19	3.0201990915241134E-4	0.0	0.0	12.778764376147675	0.0
20	3.0201990915241134E-4	0.0	0.0	13.290990142070164	0.0
21	3.0201990915241134E-4	0.0	0.0	13.68844834251474	0.0
22	3.0201990915241134E-4	0.0	0.0	14.095269160143037	0.0
23	3.0201990915241134E-4	0.0	0.0	14.514774813955736	0.0
24	3.0201990915241134E-4	0.0	0.0	14.817398762926452	0.0
25	3.0201990915241134E-4	0.0	0.0	15.06022276988499	0.0
26	3.0201990915241134E-4	0.0	0.0	15.29428819947811	0.0
27	3.0201990915241134E-4	0.0	0.0	15.679363583647435	0.0
28	3.0201990915241134E-4	0.0	0.0	15.999806707258143	0.0
29	3.0201990915241134E-4	0.0	0.0	16.294276118681744	0.0
30	3.0201990915241134E-4	0.0	0.0	16.772675654779164	0.0
31	3.0201990915241134E-4	0.0	0.0	17.034828935923457	0.0
32	3.0201990915241134E-4	0.0	0.0	17.32476804870977	0.0
33	3.0201990915241134E-4	0.0	0.0	17.613499081859477	0.0
34	3.0201990915241134E-4	0.0	0.0	17.93061998646951	0.0
35	3.0201990915241134E-4	0.0	0.0	18.25045907026191	0.0
36	3.0201990915241134E-4	0.0	0.0	18.504759833768244	0.0
37	3.0201990915241134E-4	0.0	0.0	18.77718179182372	0.0
38	3.0201990915241134E-4	0.0	0.0	19.061382526336136	0.0
39	3.0201990915241134E-4	0.0	0.0	19.39753068522277	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CCCCTCG	25	3.8847695E-5	45.000004	1
CGAACTG	30	2.160803E-6	45.000004	1
TACCCGG	30	2.160803E-6	45.000004	2
CGTGCAC	30	2.160803E-6	45.000004	19
TATGGGA	200	0.0	45.000004	4
AATGCGA	50	2.1827873E-11	45.000004	31
GCTTCGC	30	2.160803E-6	45.000004	27
TCTCGTC	25	3.8847695E-5	45.000004	12
TATTCCG	30	2.160803E-6	45.000004	1
ACAATAT	30	2.160803E-6	45.000004	22
TTGGTAG	25	3.8847695E-5	45.000004	20
GACGCAT	25	3.8847695E-5	45.000004	34
ACTCACG	25	3.8847695E-5	45.000004	1
TCAACCG	25	3.8847695E-5	45.000004	1
TACCGAG	25	3.8847695E-5	45.000004	1
ATCCTAC	20	7.026005E-4	45.0	32
AAATCCG	20	7.026005E-4	45.0	40
CTCCGTC	20	7.026005E-4	45.0	15
CGAAATG	20	7.026005E-4	45.0	1
CGAAACG	20	7.026005E-4	45.0	1
>>END_MODULE