FastQCFastQC Report
Sat 18 Jun 2016
SRR3548391_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3548391_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences335325
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTCTCTTATACACATCTGACGCTAGGATCGTCGTATGCCGTCTTCTGCT15740.4693953627078208No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA14910.4446432565421605No Hit
AAACTGTCTCTTATACACATCTGACGCTAGGATCGTCGTATGCCGTCTTCT5860.17475583389249236No Hit
AATCTGTCTCTTATACACATCTGACGCTAGGATCGTCGTATGCCGTCTTCT5000.1491090732871095No Hit
AATGATACGGCTGTCTCTTATACACATCTGACGCTAGGATCGTCGTATGCC4820.14374114664877358No Hit
AATGATACCTGTCTCTTATACACATCTGACGCTAGGATCGTCGTATGCCGT4790.1428464922090509No Hit
TGCAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGG4280.12763736673376574No Hit
AAAAACTGTCTCTTATACACATCTGACGCTAGGATCGTCGTATGCCGTCTT4020.11988369492283606No Hit
AGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT3980.11869082233653917No Hit
CCTGTCTCTTATACACATCTGACGCTAGGATCGTCGTATGCCGTCTTCTGC3470.103481696861254No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCCTA207.026131E-445.00000435
AAATCCA207.026131E-445.0000049
CACGGTT207.026131E-445.00000432
CGAAATC207.026131E-445.00000424
ACGTTTC207.026131E-445.00000419
AGATAGT207.026131E-445.00000425
ATCAGCG207.026131E-445.0000041
GATCCTA207.026131E-445.00000437
CGACATG207.026131E-445.0000041
ATCGTGT207.026131E-445.00000414
TGCATCG207.026131E-445.00000411
TAGCACG207.026131E-445.0000041
TAACCGT207.026131E-445.00000444
AACGCAC207.026131E-445.00000425
TTGCGAA207.026131E-445.00000427
CGTACTC207.026131E-445.00000445
CGCAGGA207.026131E-445.00000441
CCCTTAC207.026131E-445.00000425
CGTACCC207.026131E-445.00000429
TTAACCG207.026131E-445.00000443