##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3548391_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	335325
Sequences flagged as poor quality	0
Sequence length	51
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.878070528591664	33.0	31.0	34.0	30.0	34.0
2	32.05525385819727	33.0	31.0	34.0	30.0	34.0
3	32.195085364944454	34.0	31.0	34.0	30.0	34.0
4	35.847348095131586	37.0	35.0	37.0	35.0	37.0
5	35.640766420636695	37.0	35.0	37.0	33.0	37.0
6	35.66299261910087	37.0	35.0	37.0	33.0	37.0
7	35.73593975993439	37.0	35.0	37.0	35.0	37.0
8	35.74917169909789	37.0	35.0	37.0	35.0	37.0
9	37.382154626109	39.0	37.0	39.0	35.0	39.0
10	36.99682695892045	39.0	37.0	39.0	33.0	39.0
11	37.03327518079475	39.0	37.0	39.0	33.0	39.0
12	37.001139193319915	39.0	37.0	39.0	33.0	39.0
13	36.94666070230374	39.0	37.0	39.0	33.0	39.0
14	38.12934317453217	40.0	37.0	41.0	33.0	41.0
15	38.18428092149407	40.0	37.0	41.0	33.0	41.0
16	38.07379706255126	40.0	37.0	41.0	33.0	41.0
17	38.01934839334974	40.0	37.0	41.0	33.0	41.0
18	37.90244389771117	40.0	37.0	41.0	33.0	41.0
19	37.95846417654514	40.0	37.0	41.0	33.0	41.0
20	37.881425482740624	40.0	37.0	41.0	33.0	41.0
21	37.834986952956086	40.0	37.0	41.0	33.0	41.0
22	37.789276075449195	40.0	36.0	41.0	33.0	41.0
23	37.67502870349661	40.0	36.0	41.0	33.0	41.0
24	37.57739506448967	39.0	36.0	41.0	32.0	41.0
25	37.543445910683666	39.0	36.0	41.0	32.0	41.0
26	37.53246850070827	39.0	36.0	41.0	33.0	41.0
27	37.470966972340264	39.0	36.0	41.0	32.0	41.0
28	37.30813389994781	39.0	36.0	41.0	32.0	41.0
29	36.99543428017595	39.0	35.0	41.0	31.0	41.0
30	37.066791918288224	39.0	35.0	41.0	31.0	41.0
31	36.99940654588832	39.0	35.0	41.0	31.0	41.0
32	36.986308804890776	39.0	35.0	41.0	31.0	41.0
33	36.901731156340865	39.0	35.0	41.0	31.0	41.0
34	36.84705285916648	39.0	35.0	41.0	31.0	41.0
35	36.764586595094315	39.0	35.0	41.0	31.0	41.0
36	36.65337210169239	39.0	35.0	41.0	31.0	41.0
37	36.60741370312383	39.0	35.0	41.0	30.0	41.0
38	36.511017669425186	39.0	35.0	41.0	30.0	41.0
39	36.42139118765377	39.0	35.0	40.0	30.0	41.0
40	36.31289047938567	39.0	35.0	40.0	30.0	41.0
41	36.18440617311563	39.0	35.0	40.0	30.0	41.0
42	36.062891224931036	38.0	35.0	40.0	29.0	41.0
43	35.91136062029374	38.0	35.0	40.0	29.0	41.0
44	35.87866100052188	38.0	35.0	40.0	29.0	41.0
45	35.7348333706106	38.0	35.0	40.0	29.0	41.0
46	35.55947513606203	38.0	34.0	40.0	28.0	41.0
47	35.4152687691046	38.0	34.0	40.0	27.0	41.0
48	35.32325057779766	38.0	34.0	40.0	27.0	41.0
49	35.09056885111459	37.0	34.0	40.0	26.0	41.0
50	34.93314247371953	37.0	34.0	40.0	26.0	41.0
51	33.63611421755014	36.0	32.0	39.0	22.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	3.0
11	3.0
12	4.0
13	8.0
14	16.0
15	15.0
16	41.0
17	77.0
18	156.0
19	314.0
20	476.0
21	841.0
22	1148.0
23	1476.0
24	1875.0
25	2211.0
26	2605.0
27	2934.0
28	3833.0
29	4848.0
30	6179.0
31	7683.0
32	10087.0
33	13636.0
34	21318.0
35	26484.0
36	30377.0
37	43668.0
38	68548.0
39	84440.0
40	21.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	21.577275777231044	32.99664504585104	19.591739357339893	25.834339819578023
2	24.300603891746814	30.71736375158428	19.287855065980768	25.694177290688135
3	20.91463505554313	32.3909639901588	20.18519346902259	26.509207485275475
4	21.463654663386265	31.882800268396334	19.299187355550586	27.354357712666815
5	17.767240736598822	33.73205099530306	17.21851934690226	31.282188921195853
6	19.97375680310147	37.38432863639753	19.822858420934914	22.819056139566094
7	85.00708268098114	5.728770595690748	5.117721613360173	4.146425109967942
8	86.06098561097443	5.047938567061806	4.172370088719898	4.718705733243868
9	78.66785953925296	9.007082680981139	7.6367702974726015	4.688287482293298
10	33.510773130545	35.98658018340416	12.873182733169314	17.629463952881533
11	25.489599642138224	31.443823156639084	25.03362409602624	18.03295310519645
12	25.67091627525535	23.200178930887944	32.35219563110415	18.776709162752553
13	26.957131141429958	24.191754268247223	27.43308730336241	21.418027286960413
14	21.138000447327222	30.48684112428241	27.85804816223067	20.517110266159698
15	19.86938045180049	29.244464325654217	28.996943263997615	21.889211958547676
16	21.49854618653545	29.45709386416163	26.693208081711774	22.351151867591142
17	20.837992991873556	26.118243495116676	26.90285543875345	26.14090807425632
18	24.381719227615	26.412286587638857	25.79437858793708	23.411615596809064
19	24.141057183329607	30.37500931931708	25.09416237978081	20.389771117572504
20	24.359352866621933	27.611719973160366	24.374860210243792	23.654066949973906
21	25.97122194885559	27.38209200029822	25.334526205919627	21.312159844926565
22	21.996272273167822	28.249608588682623	24.300007455453667	25.45411168269589
23	21.807500186386342	28.62894207112503	24.014612689182137	25.548945053306493
24	19.993737418921942	28.7357041675986	24.66114963095504	26.60940878252442
25	22.148959964213823	28.585104003578614	24.288078729590694	24.977857302616865
26	23.682397673898457	27.58368746738239	23.358532766718852	25.375382092000297
27	21.104003578617757	26.298367255647502	25.433534630582272	27.164094535152465
28	23.632893461567136	27.257436815030196	26.766569745769033	22.343099977633642
29	22.64221277864758	30.788637888615522	23.870275106240214	22.69887422649668
30	25.444866920152094	26.49459479609334	26.16357265339596	21.896965630358608
31	24.688883918586445	29.10370536047118	22.57213151420264	23.635279206739728
32	24.21501528368001	29.2990382464773	23.45575188250205	23.030194587340638
33	23.67136360247521	27.012301498546186	26.47103556251398	22.845299336464624
34	23.516588384403192	27.727130395884593	24.811749794975025	23.944531424737196
35	23.94632073361664	28.461045254603746	26.52888988294938	21.06374412883024
36	24.08707969879967	28.376947737269813	26.369641392678744	21.16633117125177
37	24.682621337508387	29.210467456944755	23.091329307388357	23.0155818981585
38	24.29135912920301	27.68001192872586	25.296950719451278	22.73167822261985
39	25.48930142399165	24.581227167673152	24.960262431968985	24.96920897636621
40	23.979721166032952	25.290389920226648	26.07023037351823	24.659658540222175
41	22.253336315514797	24.935212107656753	27.098486542906137	25.712965033922313
42	25.447252665324687	26.4152687691046	24.67278013867144	23.46469842689928
43	22.433758294192202	26.035637068515623	25.939909043465292	25.590695593826883
44	24.08707969879967	23.326623425035415	26.1665548348617	26.419742041303213
45	24.544546335644522	24.266308804890777	26.617162454335347	24.571982405129354
46	22.45403712815925	25.71982405129352	28.497427868485797	23.32871095206143
47	23.451278610303437	25.80690375009319	26.312383508536495	24.429434131066877
48	22.402743606948484	24.934019235070455	27.062700365317227	25.600536792663835
49	23.82315663908149	24.912249310370534	27.07403265488705	24.190561395660925
50	22.59002460299709	23.96809065831656	27.314694699172442	26.127190039513902
51	23.88458957727578	22.86557817043167	28.099008424662642	25.15082382762991
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1814.0
1	1386.0
2	958.0
3	684.0
4	410.0
5	374.0
6	338.0
7	367.5
8	397.0
9	437.5
10	478.0
11	496.0
12	514.0
13	661.5
14	809.0
15	719.5
16	630.0
17	677.5
18	725.0
19	759.0
20	793.0
21	891.5
22	990.0
23	1314.0
24	1638.0
25	1704.5
26	1974.0
27	2177.0
28	2938.0
29	3699.0
30	4023.5
31	4348.0
32	5445.0
33	6542.0
34	7095.5
35	7649.0
36	8332.5
37	9016.0
38	9268.0
39	9520.0
40	11401.5
41	13283.0
42	14378.0
43	15473.0
44	18618.5
45	21764.0
46	24755.0
47	27746.0
48	29893.0
49	32040.0
50	30785.0
51	29530.0
52	27502.0
53	25474.0
54	23315.0
55	21156.0
56	19108.0
57	17060.0
58	17190.0
59	17320.0
60	16813.5
61	16307.0
62	14423.0
63	12539.0
64	10696.5
65	8854.0
66	7618.5
67	6383.0
68	5564.0
69	4745.0
70	4189.5
71	3634.0
72	3273.5
73	2913.0
74	2161.5
75	1265.5
76	1121.0
77	881.5
78	642.0
79	450.5
80	259.0
81	202.0
82	145.0
83	154.5
84	164.0
85	107.5
86	51.0
87	59.0
88	67.0
89	44.0
90	21.0
91	13.5
92	6.0
93	3.0
94	0.0
95	0.5
96	1.0
97	1.0
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	335325.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	30.07014332581082
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.02574641686768	23.462453778585814
2	8.853371033829793	5.324442718076877
3	3.00982090250929	2.715172377704269
4	1.4658653074876504	1.763151195699496
5	0.875918601546164	1.3169498945118465
6	0.5749465513292744	1.0373235121891158
7	0.4629569616996795	0.9744827534393893
8	0.42496049398597047	1.02228983695724
9	0.290972949999479	0.7874638479376488
>10	4.893545076623357	35.80720490679308
>50	1.0329039771458781	20.354392000757954
>100	0.08599200584176721	4.336914296545331
>500	9.999070446717118E-4	0.1761946601341905
>1k	0.0019998140893434237	0.9215642206677371
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTGTCTCTTATACACATCTGACGCTAGGATCGTCGTATGCCGTCTTCTGCT	1574	0.4693953627078208	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1491	0.4446432565421605	No Hit
AAACTGTCTCTTATACACATCTGACGCTAGGATCGTCGTATGCCGTCTTCT	586	0.17475583389249236	No Hit
AATCTGTCTCTTATACACATCTGACGCTAGGATCGTCGTATGCCGTCTTCT	500	0.1491090732871095	No Hit
AATGATACGGCTGTCTCTTATACACATCTGACGCTAGGATCGTCGTATGCC	482	0.14374114664877358	No Hit
AATGATACCTGTCTCTTATACACATCTGACGCTAGGATCGTCGTATGCCGT	479	0.1428464922090509	No Hit
TGCAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGG	428	0.12763736673376574	No Hit
AAAAACTGTCTCTTATACACATCTGACGCTAGGATCGTCGTATGCCGTCTT	402	0.11988369492283606	No Hit
AGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT	398	0.11869082233653917	No Hit
CCTGTCTCTTATACACATCTGACGCTAGGATCGTCGTATGCCGTCTTCTGC	347	0.103481696861254	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.546037426377395	0.0
2	0.0	0.0	0.0	0.8075747409229852	0.0
3	0.0	0.0	0.0	1.1031089241780363	0.0
4	0.0	0.0	0.0	2.4006560799224634	0.0
5	0.0	0.0	0.0	2.6753149929173192	0.0
6	0.0	0.0	0.0	3.8658018340416014	0.0
7	0.0	0.0	0.0	4.682323119361813	0.0
8	0.0	0.0	0.0	5.478267352568404	0.0
9	0.0	0.0	0.0	6.428390367553866	0.0
10	0.0	0.0	0.0	7.968090658316559	0.0
11	0.0	0.0	0.0	9.22716767315291	0.0
12	0.0	0.0	0.0	9.94318944307761	0.0
13	0.0	0.0	0.0	10.352941176470589	0.0
14	0.0	0.0	0.0	10.709610079773354	0.0
15	0.0	0.0	0.0	10.964288376947737	0.0
16	0.0	0.0	0.0	11.49392380526355	0.0
17	0.0	0.0	0.0	12.128830239320063	0.0
18	0.0	0.0	0.0	13.200328039961231	0.0
19	0.0	0.0	0.0	13.543278908521584	0.0
20	0.0	0.0	0.0	14.105420114813986	0.0
21	0.0	0.0	0.0	14.527100574069932	0.0
22	0.0	0.0	0.0	14.95385074181764	0.0
23	0.0	0.0	0.0	15.399686870946097	0.0
24	0.0	0.0	0.0	15.701483635279207	0.0
25	0.0	0.0	0.0	15.968090658316559	0.0
26	0.0	0.0	0.0	16.211734884067695	0.0
27	0.0	0.0	0.0	16.58242004025945	0.0
28	0.0	0.0	0.0	16.9253709088198	0.0
29	0.0	0.0	0.0	17.211660329531053	0.0
30	0.0	0.0	0.0	17.68791470961008	0.0
31	0.0	0.0	0.0	17.97002907626929	0.0
32	0.0	0.0	0.0	18.247968388876462	0.0
33	0.0	0.0	0.0	18.566465369417728	0.0
34	0.0	0.0	0.0	18.886453440691866	0.0
35	0.0	0.0	0.0	19.210020129724892	0.0
36	0.0	0.0	0.0	19.46768060836502	0.0
37	0.0	0.0	0.0	19.742041303213302	0.0
38	0.0	0.0	0.0	20.019682397673897	0.0
39	0.0	0.0	0.0	20.34652948631924	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGCCTA	20	7.026131E-4	45.000004	35
AAATCCA	20	7.026131E-4	45.000004	9
CACGGTT	20	7.026131E-4	45.000004	32
CGAAATC	20	7.026131E-4	45.000004	24
ACGTTTC	20	7.026131E-4	45.000004	19
AGATAGT	20	7.026131E-4	45.000004	25
ATCAGCG	20	7.026131E-4	45.000004	1
GATCCTA	20	7.026131E-4	45.000004	37
CGACATG	20	7.026131E-4	45.000004	1
ATCGTGT	20	7.026131E-4	45.000004	14
TGCATCG	20	7.026131E-4	45.000004	11
TAGCACG	20	7.026131E-4	45.000004	1
TAACCGT	20	7.026131E-4	45.000004	44
AACGCAC	20	7.026131E-4	45.000004	25
TTGCGAA	20	7.026131E-4	45.000004	27
CGTACTC	20	7.026131E-4	45.000004	45
CGCAGGA	20	7.026131E-4	45.000004	41
CCCTTAC	20	7.026131E-4	45.000004	25
CGTACCC	20	7.026131E-4	45.000004	29
TTAACCG	20	7.026131E-4	45.000004	43
>>END_MODULE