FastQCFastQC Report
Sat 18 Jun 2016
SRR3548390_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3548390_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences659087
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA146112.216854527550991No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA38760.588086246580497No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA27170.4122369277500542No Hit
CTGTCTCTTATACACATCTGACGCCGATTGGATCGTATGCCGTCTTCTGCT20730.3145260033956064Illumina Single End Adapter 2 (95% over 21bp)
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA17260.2618774152729457No Hit
AATGATACCTGTCTCTTATACACATCTGACGCCGATTGGATCGTATGCCGT7020.10651097654786089No Hit
TGCAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGG6710.10180750037551947No Hit
AATGATACGGCTGTCTCTTATACACATCTGACGCCGATTGGATCGTATGCC6680.10135232526206708No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGATCGA253.888987E-545.00000423
GGGTGCG302.1640863E-645.0000047
ACCGGAC302.1640863E-645.00000419
GTATTGG302.1640863E-645.00000443
GTCGACC302.1640863E-645.00000431
CGAAACG253.888987E-545.0000041
GTGATCG302.1640863E-645.00000436
CACGACA302.1640863E-645.00000415
AATGCGG253.888987E-545.0000042
CTTATTG253.888987E-545.00000442
TATCTCG302.1640863E-645.0000041
GTTAACG302.1640863E-645.0000041
CGCTAGC253.888987E-545.00000422
CCGAGTT253.888987E-545.00000429
ACAATCT253.888987E-545.00000438
TATTACG253.888987E-545.0000041
GAACGTT253.888987E-545.00000431
CGAATCA302.1640863E-645.00000441
CCCTTAT253.888987E-545.00000412
TCATCCG253.888987E-545.0000041