##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3548390_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	659087
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.148491777261576	31.0	31.0	34.0	28.0	34.0
2	31.312608198917594	31.0	31.0	34.0	28.0	34.0
3	31.508222738424518	31.0	31.0	34.0	30.0	34.0
4	35.468323605229656	37.0	35.0	37.0	33.0	37.0
5	35.05031657429141	37.0	35.0	37.0	32.0	37.0
6	35.04412922724921	36.0	35.0	37.0	32.0	37.0
7	34.246126839097116	36.0	35.0	37.0	31.0	37.0
8	34.601048116561245	37.0	35.0	37.0	32.0	37.0
9	35.744668002858496	39.0	35.0	39.0	31.0	39.0
10	35.43332670800668	37.0	35.0	39.0	30.0	39.0
11	35.864266781168496	37.0	35.0	39.0	31.0	39.0
12	35.63968034569032	37.0	35.0	39.0	30.0	39.0
13	35.32744235586501	37.0	35.0	39.0	30.0	39.0
14	36.365387270572775	38.0	35.0	40.0	30.0	41.0
15	36.51364083952498	38.0	35.0	40.0	31.0	41.0
16	36.53929602616954	38.0	35.0	40.0	31.0	41.0
17	36.45682133011272	38.0	35.0	40.0	31.0	41.0
18	36.31571552769209	38.0	35.0	40.0	30.0	41.0
19	36.22505830034578	38.0	34.0	40.0	30.0	41.0
20	36.14507796391068	38.0	34.0	40.0	30.0	41.0
21	36.122800176607946	38.0	34.0	40.0	30.0	41.0
22	36.111662041581766	38.0	34.0	40.0	30.0	41.0
23	36.003362226838036	38.0	34.0	40.0	30.0	41.0
24	35.63424100308457	37.0	34.0	40.0	29.0	41.0
25	35.72957439609642	38.0	34.0	40.0	29.0	41.0
26	35.575089479841054	37.0	34.0	40.0	29.0	41.0
27	35.3621949757771	37.0	34.0	40.0	28.0	41.0
28	35.29341649888406	37.0	34.0	40.0	27.0	41.0
29	35.074987065440524	37.0	34.0	40.0	27.0	41.0
30	35.07194801293304	37.0	34.0	40.0	27.0	41.0
31	34.95956982917278	37.0	33.0	40.0	27.0	41.0
32	34.81370441231582	37.0	33.0	40.0	26.0	41.0
33	34.73984466390628	37.0	33.0	40.0	25.0	41.0
34	34.665158014040635	37.0	33.0	40.0	25.0	41.0
35	34.49281809533491	37.0	33.0	40.0	24.0	41.0
36	34.33476157168932	36.0	33.0	40.0	24.0	41.0
37	34.17338833871705	36.0	33.0	40.0	23.0	41.0
38	34.057643376367615	36.0	33.0	40.0	23.0	41.0
39	34.00328939881988	36.0	33.0	40.0	23.0	41.0
40	33.7795799340603	36.0	33.0	40.0	23.0	41.0
41	33.713974027708026	36.0	33.0	39.0	22.0	41.0
42	33.59889058652348	35.0	33.0	39.0	22.0	41.0
43	33.53701711610152	35.0	33.0	39.0	22.0	41.0
44	33.421212980987335	35.0	32.0	39.0	22.0	40.0
45	33.20914082662835	35.0	32.0	39.0	22.0	40.0
46	33.07460471834523	35.0	32.0	39.0	20.0	40.0
47	32.91374279875039	35.0	32.0	39.0	20.0	40.0
48	32.7761873622147	35.0	31.0	39.0	20.0	40.0
49	32.59590160327848	35.0	31.0	39.0	19.0	40.0
50	32.42106580770065	35.0	31.0	39.0	18.0	40.0
51	30.431251109489338	34.0	27.0	37.0	11.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	5.0
9	6.0
10	18.0
11	20.0
12	21.0
13	30.0
14	60.0
15	137.0
16	249.0
17	539.0
18	959.0
19	1711.0
20	2655.0
21	3800.0
22	5062.0
23	6416.0
24	7504.0
25	8785.0
26	9954.0
27	11800.0
28	14053.0
29	17054.0
30	21419.0
31	26908.0
32	34325.0
33	46519.0
34	70509.0
35	70309.0
36	66423.0
37	85642.0
38	96132.0
39	50062.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	20.921365464650343	32.995947424239894	19.834559018763837	26.24812809234593
2	23.75392019566461	33.840904159845365	19.77007587769141	22.635099766798618
3	20.349513797116316	34.512590902263284	19.60075073548712	25.537144565133286
4	20.181705905290197	35.60167322371705	18.588593008206807	25.628027862785945
5	17.616794140985938	38.29555127016616	16.653795325958484	27.43385926288942
6	19.110982313412343	39.68459399138505	18.926181217350667	22.278242477851936
7	79.69767268964492	12.97446315888494	3.9929478202422444	3.3349163312278955
8	83.03714077200733	10.788560539048714	3.0722802907658626	3.102018398178086
9	77.31164474492745	14.725976995449766	4.967781188219461	2.99459707140332
10	41.100036869184194	36.8226046030342	9.170413010725442	12.90694551705617
11	33.79644872376485	29.922453333171497	21.21055338673043	15.070544556333232
12	33.849552487000956	27.85656521824888	22.71581748691751	15.578064807832654
13	23.426649289092335	36.96355716316662	20.675115728272594	18.934677819468444
14	17.473262255210617	37.37200096497124	25.7527458438719	19.40199093594624
15	17.847416198468487	31.788823023364138	32.19726682516876	18.166493952998618
16	19.758544774817285	29.436478037042153	30.519946532096675	20.28503065604389
17	19.19412763413631	29.80577677908986	25.25721187036006	25.74288371641377
18	22.890301280407595	30.31177978021111	24.749236443747183	22.04868249563411
19	23.787754879097903	33.57705431908079	21.632348991862987	21.002841809958323
20	27.263623770458224	31.152791664833323	21.69577005767069	19.887814507037767
21	22.851914845839776	33.17134156795689	22.964646548938152	21.012097037265185
22	22.143055469156575	29.849777040056928	23.209530759975543	24.797636730810954
23	21.888157405623232	34.04952608684438	22.073261951760543	21.989054555771848
24	21.094787182875706	31.002735602431848	27.64399844026661	20.258478774425832
25	20.779350829253193	31.1265432332909	24.346103018266177	23.74800291918973
26	22.79820418245239	35.499865723341536	20.717447013823666	20.984483080382407
27	19.18487240682945	34.30761037617189	23.788210054211355	22.71930716278731
28	19.904200811122053	33.928752956741675	27.27773419897525	18.889312033161023
29	20.06654660158674	32.44624761222722	26.133879138869375	21.353326647316667
30	23.151268345453634	31.845568187507872	24.753181294730435	20.249982172308055
31	26.596640504212644	30.09344745079178	22.304187459318726	21.00572458567685
32	26.27088684801855	31.798229975708818	23.317710711939394	18.613172464333235
33	25.562937821562254	30.936583485943437	21.84339851946708	21.657080173027232
34	22.21391106181733	32.14188718636538	23.438028666928645	22.206173084888643
35	22.71703128722005	31.854064789625646	24.940106541321555	20.488797381832747
36	26.634420038629198	30.98285962247776	22.979970777757718	19.402749561135327
37	22.866480449470252	34.23599615832204	22.64207911853822	20.255444273669486
38	23.766816823879093	31.84571991254569	23.138523442276966	21.24893982129825
39	24.219867786802045	29.14015903818464	24.665180772796308	21.974792402217005
40	25.729076737972377	28.366513070353385	23.452442545521304	22.451967646152937
41	20.8914756322003	28.625052534794342	25.34566756740764	25.137804265597712
42	24.628615038682298	29.452409166012984	22.424960589421428	23.49401520588329
43	23.370359300062056	28.664804494702523	23.572153600359286	24.39268260487614
44	22.58093392829778	30.9015350022076	23.519353287198808	22.998177782295812
45	22.330587615898963	32.204246176908356	23.491435880240395	21.973730326952285
46	22.907446209680966	30.334386810845913	25.09607988019791	21.662087099275208
47	22.206476534964274	31.364599817626505	24.042349492555612	22.38657415485361
48	23.549546569724484	28.617618007941285	27.097181403972463	20.735654018361764
49	22.664382699097388	27.751723217117014	25.907201932370082	23.67669215141552
50	20.857792673804823	31.088156798723084	24.00335615783652	24.050694369635572
51	20.808330311476332	29.741293638017442	25.20441155719958	24.245964493306648
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	17071.0
1	13474.5
2	9878.0
3	6405.0
4	2932.0
5	2447.5
6	1963.0
7	2014.5
8	2066.0
9	2123.5
10	2181.0
11	2373.0
12	2565.0
13	2579.5
14	2594.0
15	2549.5
16	2505.0
17	2857.0
18	3209.0
19	2983.0
20	2757.0
21	2499.0
22	2241.0
23	2634.0
24	3027.0
25	3400.5
26	3835.0
27	3896.0
28	5496.5
29	7097.0
30	8686.0
31	10275.0
32	11616.0
33	12957.0
34	14123.0
35	15289.0
36	17125.5
37	18962.0
38	20332.0
39	21702.0
40	24588.0
41	27474.0
42	32207.0
43	36940.0
44	42522.0
45	48104.0
46	48566.5
47	49029.0
48	49091.0
49	49153.0
50	46485.0
51	43817.0
52	41323.5
53	38830.0
54	36770.5
55	34711.0
56	32324.0
57	29937.0
58	28792.5
59	27648.0
60	28749.0
61	29850.0
62	27656.5
63	25463.0
64	22937.0
65	20411.0
66	17435.5
67	14460.0
68	12803.0
69	11146.0
70	9347.5
71	7549.0
72	6606.0
73	5663.0
74	4775.5
75	3341.5
76	2795.0
77	2062.5
78	1330.0
79	1063.5
80	797.0
81	663.5
82	530.0
83	381.0
84	232.0
85	185.5
86	139.0
87	114.5
88	90.0
89	75.5
90	61.0
91	50.0
92	39.0
93	39.5
94	40.0
95	24.5
96	9.0
97	9.0
98	9.0
99	5.5
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	659087.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	25.65807684402921
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.98718985971452	20.77975540802538
2	8.30030086984373	4.259395150940259
3	2.9071839294725565	2.2377824598640093
4	1.3777192903719262	1.4139850968745709
5	0.8033729581560477	1.0306502547391465
6	0.5067192383282745	0.7800864693224892
7	0.3829565636216054	0.687815025612996
8	0.27697332558740284	0.5685282297334324
9	0.23784175429103793	0.5492305807486348
>10	2.4387873951225494	14.75367960228254
>50	0.9650374629886301	18.12841547355146
>100	0.808037021459701	30.138747355802426
>500	0.004849434487380051	0.7831134554148358
>1k	0.0024247172436900255	1.6163088438274031
>5k	0.0	0.0
>10k+	6.061793109225064E-4	2.2725065932604105
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	14611	2.216854527550991	No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	3876	0.588086246580497	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2717	0.4122369277500542	No Hit
CTGTCTCTTATACACATCTGACGCCGATTGGATCGTATGCCGTCTTCTGCT	2073	0.3145260033956064	Illumina Single End Adapter 2 (95% over 21bp)
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1726	0.2618774152729457	No Hit
AATGATACCTGTCTCTTATACACATCTGACGCCGATTGGATCGTATGCCGT	702	0.10651097654786089	No Hit
TGCAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGG	671	0.10180750037551947	No Hit
AATGATACGGCTGTCTCTTATACACATCTGACGCCGATTGGATCGTATGCC	668	0.10135232526206708	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.5172503781746567E-4	0.0	0.0	0.4976581240412874	0.0
2	1.5172503781746567E-4	0.0	0.0	0.6075070514211326	0.0
3	1.5172503781746567E-4	0.0	0.0	0.7522527374989948	0.0
4	1.5172503781746567E-4	0.0	0.0	1.5670161905787856	0.0
5	1.5172503781746567E-4	0.0	0.0	1.6748926924670036	0.0
6	1.5172503781746567E-4	0.0	0.0	2.23308910659746	0.0
7	1.5172503781746567E-4	0.0	0.0	2.674002066495015	0.0
8	1.5172503781746567E-4	0.0	0.0	2.943465733658834	0.0
9	1.5172503781746567E-4	0.0	0.0	3.52502780361318	0.0
10	1.5172503781746567E-4	0.0	0.0	4.254521785439555	0.0
11	1.5172503781746567E-4	0.0	0.0	5.071561114086608	0.0
12	1.5172503781746567E-4	0.0	0.0	5.456942710142971	0.0
13	1.5172503781746567E-4	0.0	0.0	5.609426373149524	0.0
14	1.5172503781746567E-4	0.0	0.0	5.786944667395958	0.0
15	1.5172503781746567E-4	0.0	0.0	5.917883375032432	0.0
16	1.5172503781746567E-4	0.0	0.0	6.173691788792678	0.0
17	1.5172503781746567E-4	0.0	0.0	6.499748895062412	0.0
18	1.5172503781746567E-4	0.0	0.0	6.935351478636356	0.0
19	1.5172503781746567E-4	0.0	0.0	7.1181801492064025	0.0
20	1.5172503781746567E-4	0.0	0.0	7.312843372726211	0.0
21	1.5172503781746567E-4	0.0	0.0	7.527686026275742	0.0
22	1.5172503781746567E-4	0.0	0.0	7.739494179068924	0.0
23	1.5172503781746567E-4	0.0	0.0	7.968902436248932	0.0
24	1.5172503781746567E-4	0.0	0.0	8.135193077696874	0.0
25	1.5172503781746567E-4	0.0	0.0	8.272200786846046	0.0
26	1.5172503781746567E-4	0.0	0.0	8.4090567709574	0.0
27	1.5172503781746567E-4	0.0	0.0	8.548188630636016	0.0
28	1.5172503781746567E-4	0.0	0.0	8.695361917318959	0.0
29	1.5172503781746567E-4	0.0	0.0	8.85088008108186	0.0
30	1.5172503781746567E-4	0.0	0.0	9.070426210803733	0.0
31	1.5172503781746567E-4	0.0	0.0	9.225489199453182	0.0
32	1.5172503781746567E-4	0.0	0.0	9.391324665787673	0.0
33	1.5172503781746567E-4	0.0	0.0	9.54957388023129	0.0
34	1.5172503781746567E-4	0.0	0.0	9.711616220620343	0.0
35	1.5172503781746567E-4	0.0	0.0	9.896417316682015	0.0
36	1.5172503781746567E-4	0.0	0.0	10.05724585676853	0.0
37	1.5172503781746567E-4	0.0	0.0	10.218377846930679	0.0
38	1.5172503781746567E-4	0.0	0.0	10.387247814021517	0.0
39	1.5172503781746567E-4	0.0	0.0	10.586766238751485	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TGATCGA	25	3.888987E-5	45.000004	23
GGGTGCG	30	2.1640863E-6	45.000004	7
ACCGGAC	30	2.1640863E-6	45.000004	19
GTATTGG	30	2.1640863E-6	45.000004	43
GTCGACC	30	2.1640863E-6	45.000004	31
CGAAACG	25	3.888987E-5	45.000004	1
GTGATCG	30	2.1640863E-6	45.000004	36
CACGACA	30	2.1640863E-6	45.000004	15
AATGCGG	25	3.888987E-5	45.000004	2
CTTATTG	25	3.888987E-5	45.000004	42
TATCTCG	30	2.1640863E-6	45.000004	1
GTTAACG	30	2.1640863E-6	45.000004	1
CGCTAGC	25	3.888987E-5	45.000004	22
CCGAGTT	25	3.888987E-5	45.000004	29
ACAATCT	25	3.888987E-5	45.000004	38
TATTACG	25	3.888987E-5	45.000004	1
GAACGTT	25	3.888987E-5	45.000004	31
CGAATCA	30	2.1640863E-6	45.000004	41
CCCTTAT	25	3.888987E-5	45.000004	12
TCATCCG	25	3.888987E-5	45.000004	1
>>END_MODULE