FastQCFastQC Report
Sat 18 Jun 2016
SRR3548387_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3548387_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences564504
Sequences flagged as poor quality0
Sequence length46
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA265284.699346683105877No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA66101.1709394441846293No Hit
CTGTCTCTTATACACATCTGACGCACTCACCATCGTATGCCGTCTT56060.9930841942661169No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA47000.8325893173476184No Hit
AATGATACCTGTCTCTTATACACATCTGACGCACTCACCATCGTAT46710.8274520641129204No Hit
AATGATACGGCTGTCTCTTATACACATCTGACGCACTCACCATCGT46200.8184175842863824No Hit
AATCTGTCTCTTATACACATCTGACGCACTCACCATCGTATGCCGT44550.789188384847583No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA24840.44003231155137956No Hit
AAACTGTCTCTTATACACATCTGACGCACTCACCATCGTATGCCGT12230.21665036917364625No Hit
AATGACTGTCTCTTATACACATCTGACGCACTCACCATCGTATGCC10340.18316964981647607No Hit
AACTGTCTCTTATACACATCTGACGCACTCACCATCGTATGCCGTC9340.16545498348993098No Hit
AAAAACTGTCTCTTATACACATCTGACGCACTCACCATCGTATGCC9310.16492354350013463No Hit
AATGATCTGTCTCTTATACACATCTGACGCACTCACCATCGTATGC8390.14862605047971317No Hit
AGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA6960.12329407763275371No Hit
AATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCACTCA6210.11000807788784489No Hit
CCTGTCTCTTATACACATCTGACGCACTCACCATCGTATGCCGTCT6060.10735087793886315No Hit
ACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA5970.10575655796947409No Hit
AATGATACGGCGACTGTCTCTTATACACATCTGACGCACTCACCAT5880.10416223800008503No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGAGC555.456968E-1240.00000417
GAGCGCG555.456968E-1240.00000420
CTAGAGA200.001255226240.014
AACGTAG200.001255226240.020
CTAGACG401.9254003E-840.01
AACCGCG200.001255226240.01
TCGTTGA900.040.024
AGCCGAC257.789607E-540.038
CGAGTTT304.863108E-640.032
CTACTAC200.001255226240.011
AATCACG304.863108E-640.01
CGAAACG200.001255226240.01
CAACGAC257.789607E-540.010
CAACGAA257.789607E-540.012
AACCCGA304.863108E-640.024
TCCGACA200.001255226240.023
GCGCGAC200.001255226240.09
CGTGATT200.001255226240.040
ACCGCAA200.001255226240.028
CGCATCG257.789607E-540.01