##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3548387_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	564504
Sequences flagged as poor quality	0
Sequence length	46
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.922028896163713	31.0	31.0	33.0	28.0	34.0
2	31.235629862675907	31.0	31.0	34.0	28.0	34.0
3	31.433738290605557	31.0	31.0	34.0	30.0	34.0
4	35.40668268072503	37.0	35.0	37.0	33.0	37.0
5	34.93246992049658	35.0	35.0	37.0	32.0	37.0
6	35.074787778297406	35.0	35.0	37.0	32.0	37.0
7	34.901495826424615	36.0	35.0	37.0	32.0	37.0
8	35.256283392146024	37.0	35.0	37.0	33.0	37.0
9	36.66969941754177	39.0	35.0	39.0	32.0	39.0
10	35.94410314187322	37.0	35.0	39.0	31.0	39.0
11	36.20257960971047	37.0	35.0	39.0	32.0	39.0
12	35.884790896078684	37.0	35.0	39.0	31.0	39.0
13	35.5167899607443	37.0	35.0	39.0	30.0	39.0
14	36.67932911015688	38.0	35.0	40.0	31.0	41.0
15	36.68859919504556	38.0	35.0	40.0	31.0	41.0
16	36.54632208097728	38.0	35.0	40.0	31.0	41.0
17	36.506929977466946	38.0	35.0	40.0	31.0	41.0
18	36.24057048311438	38.0	35.0	40.0	31.0	41.0
19	36.294015631421566	38.0	34.0	40.0	31.0	41.0
20	36.09645104374814	38.0	34.0	40.0	30.0	41.0
21	36.2300036846506	38.0	34.0	40.0	31.0	41.0
22	36.28468177373411	38.0	35.0	40.0	31.0	41.0
23	36.07889758088517	38.0	34.0	40.0	31.0	41.0
24	35.63632852911582	37.0	34.0	40.0	29.0	41.0
25	35.84431111205589	37.0	34.0	40.0	30.0	41.0
26	35.806052747190456	37.0	34.0	40.0	30.0	41.0
27	35.6888613721072	37.0	34.0	40.0	30.0	41.0
28	35.67482604197667	37.0	34.0	40.0	30.0	41.0
29	35.4716441336111	37.0	34.0	40.0	29.0	41.0
30	35.46806045661324	37.0	34.0	40.0	29.0	41.0
31	35.34285319501722	37.0	34.0	40.0	29.0	41.0
32	35.2218124229412	37.0	34.0	40.0	28.0	41.0
33	35.21858126780324	37.0	34.0	40.0	28.0	41.0
34	35.156206156200845	37.0	34.0	40.0	27.0	41.0
35	34.93603942576138	37.0	34.0	40.0	26.0	41.0
36	34.952359947848024	37.0	34.0	40.0	26.0	41.0
37	34.80809170528464	37.0	34.0	40.0	25.0	41.0
38	34.62449690347633	37.0	33.0	40.0	25.0	41.0
39	34.53136558819778	37.0	33.0	40.0	24.0	41.0
40	34.34306045661324	36.0	33.0	40.0	24.0	41.0
41	34.470072842707935	37.0	33.0	40.0	24.0	41.0
42	34.29520428553208	36.0	33.0	40.0	24.0	41.0
43	34.28954622110738	36.0	33.0	40.0	23.0	41.0
44	34.22865028414325	36.0	33.0	40.0	23.0	41.0
45	34.03779601207432	36.0	33.0	40.0	23.0	41.0
46	32.94938388390516	35.0	31.0	39.0	21.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	3.0
9	6.0
10	13.0
11	16.0
12	14.0
13	16.0
14	30.0
15	47.0
16	68.0
17	135.0
18	278.0
19	538.0
20	1099.0
21	1826.0
22	2876.0
23	3934.0
24	5204.0
25	6122.0
26	7027.0
27	8049.0
28	9303.0
29	11783.0
30	15185.0
31	19877.0
32	26739.0
33	36942.0
34	59125.0
35	70001.0
36	58628.0
37	73557.0
38	92179.0
39	53883.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	16.17933619602341	38.74658106939898	19.11394781967887	25.96013491489874
2	19.71819508807732	40.3159233592676	18.302970395249634	21.66291115740544
3	17.76462168558593	39.082096849623746	20.73572552187407	22.41755594291626
4	19.50491050550572	38.49042699431713	16.841156129983137	25.16350637019401
5	14.855696328103965	43.333971061321094	16.344436886186813	25.465895724388137
6	18.1139549055454	41.05604211839066	20.10774060059805	20.722262375465895
7	72.4869974349163	17.60146960871845	5.366835310290095	4.5446976460751385
8	74.76297776455083	14.268986579368793	4.148420560350325	6.8196150957300565
9	70.78320082762922	17.87020109689214	7.555482334934739	3.7911157405439115
10	34.712951546844664	43.92050366339299	10.11418873914091	11.25235605062143
11	26.69741932740955	30.963465272168133	27.032403667644516	15.3067117327778
12	27.20919603758344	31.22670521378059	26.766506526083074	14.797592222552897
13	19.651233649362982	41.41210691155421	21.880624406558677	17.05603503252413
14	13.879441066848063	41.62468290747275	27.898473704349303	16.597402321329877
15	13.209649533041395	37.65287757039808	29.881453452942758	19.25601944361776
16	15.659765032665845	35.82011819225373	30.161522327565436	18.358594447514985
17	14.607159559542538	35.108520045916414	24.441456570724036	25.842863823817012
18	17.374721879738672	34.92375607613055	25.1066422912858	22.594879752844975
19	18.619531482504996	39.05180477020535	23.97361223303998	18.35505151424968
20	20.88594589232317	38.52532488698043	22.810998682028824	17.777730538667573
21	16.696427306095263	37.5543840256225	24.718159658744668	21.031029009537576
22	17.71679208650426	36.254658957243876	24.74614883154061	21.282400124711252
23	18.69251590777036	37.4123124016836	20.788160934200643	23.107010756345396
24	15.26543656023695	40.04825475107351	24.952347547581592	19.733961141107947
25	15.335409492226804	37.49450845343877	25.065544265408217	22.10453778892621
26	18.559124470331476	41.520697816135936	20.262921077618582	19.657256635914006
27	13.945339625582811	40.033374431359206	25.833475050663946	20.187810892394033
28	14.914154726981563	39.1315207686748	26.241089522837747	19.713234981505888
29	15.640810339696444	37.09025976786701	26.044456726613095	21.224473165823447
30	17.087389991922112	36.9124045179485	25.470678684296306	20.529526805833083
31	21.015085809843686	37.5779445318368	18.95965307597466	22.447316582344857
32	19.939982710485666	38.78094752207247	20.301007610220655	20.978062157221206
33	19.17772061845443	37.67714666326545	20.444673554128933	22.700459164151184
34	15.798825163329225	38.12621345464337	20.658489576690332	25.416471805337075
35	16.914671995238297	38.729752136388754	22.65351529838584	21.702060569987104
36	19.2112013378116	33.74803367203775	24.603722914275185	22.43704207587546
37	16.285092753992885	36.986451823193455	23.6935433584173	23.034912064396355
38	17.507050437197964	36.28371101001942	22.63137196547766	23.57786658730496
39	17.583754942391906	35.8156895256721	24.051202471550244	22.549353060385755
40	19.297117469495344	35.67326360840667	20.508800646231027	24.520818275866958
41	16.67446111985035	31.48427646216856	25.77129657185777	26.06996584612332
42	21.026068902966145	35.0968283661409	21.1293454076499	22.74775732324306
43	19.21509856440344	32.807916330088005	24.473342970111815	23.50364213539674
44	18.64344628204583	34.63713277496705	22.833496308263538	23.88592463472358
45	21.449626574833836	36.366261355101116	20.944404291200772	21.23970777886428
46	18.498894604821224	34.90232132987543	26.050302566500854	20.548481498802488
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	29971.0
1	23029.0
2	16087.0
3	10241.5
4	4396.0
5	3725.0
6	3054.0
7	3054.0
8	2987.5
9	2921.0
10	3052.0
11	3183.0
12	3379.5
13	3576.0
14	3738.5
15	3901.0
16	3933.5
17	3966.0
18	3883.0
19	3800.0
20	3800.0
21	3654.5
22	3509.0
23	3619.0
24	3729.0
25	3838.5
26	3948.0
27	4634.0
28	5320.0
29	6385.0
30	7450.0
31	7450.0
32	8586.0
33	9722.0
34	11949.0
35	14176.0
36	15633.5
37	17091.0
38	18817.5
39	20544.0
40	25862.0
41	31180.0
42	34493.0
43	37806.0
44	37806.0
45	50280.0
46	62754.0
47	63221.0
48	63688.0
49	58000.5
50	52313.0
51	45906.0
52	39499.0
53	34285.0
54	29071.0
55	25856.0
56	22641.0
57	22641.0
58	19414.5
59	16188.0
60	15773.5
61	15359.0
62	13831.5
63	12304.0
64	9806.5
65	7309.0
66	5953.5
67	4598.0
68	4349.0
69	4100.0
70	4100.0
71	2966.0
72	1832.0
73	1542.5
74	1253.0
75	1055.5
76	858.0
77	772.0
78	686.0
79	526.5
80	367.0
81	367.0
82	261.0
83	155.0
84	129.0
85	103.0
86	69.5
87	36.0
88	34.5
89	33.0
90	19.0
91	5.0
92	5.0
93	5.0
94	5.0
95	6.5
96	8.0
97	8.0
98	8.0
99	4.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
46	564504.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	25.491187007146383
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.58199025894629	19.266746480631163
2	10.017300673889027	5.107057695698374
3	3.7902932228884216	2.8985722006970494
4	1.9327934114358583	1.9707679318836755
5	1.1893125348277391	1.5158494117618597
6	0.8724626404708666	1.334406499499495
7	0.6175624513794551	1.1019679955693804
8	0.48743245141732694	0.99401854179447
9	0.407830329002603	0.935647126541225
>10	3.7793528343094596	21.125813468835034
>50	0.8901135110373415	16.097497704778462
>100	0.4210090529886878	15.614912147131099
>500	0.005576278847532289	1.0326904495752887
>1k	0.004879243991590752	4.119923168324538
>5k	0.0013940697118830722	2.170568914661343
>10k+	6.970348559415361E-4	4.71356026261756
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	26528	4.699346683105877	No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	6610	1.1709394441846293	No Hit
CTGTCTCTTATACACATCTGACGCACTCACCATCGTATGCCGTCTT	5606	0.9930841942661169	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	4700	0.8325893173476184	No Hit
AATGATACCTGTCTCTTATACACATCTGACGCACTCACCATCGTAT	4671	0.8274520641129204	No Hit
AATGATACGGCTGTCTCTTATACACATCTGACGCACTCACCATCGT	4620	0.8184175842863824	No Hit
AATCTGTCTCTTATACACATCTGACGCACTCACCATCGTATGCCGT	4455	0.789188384847583	No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2484	0.44003231155137956	No Hit
AAACTGTCTCTTATACACATCTGACGCACTCACCATCGTATGCCGT	1223	0.21665036917364625	No Hit
AATGACTGTCTCTTATACACATCTGACGCACTCACCATCGTATGCC	1034	0.18316964981647607	No Hit
AACTGTCTCTTATACACATCTGACGCACTCACCATCGTATGCCGTC	934	0.16545498348993098	No Hit
AAAAACTGTCTCTTATACACATCTGACGCACTCACCATCGTATGCC	931	0.16492354350013463	No Hit
AATGATCTGTCTCTTATACACATCTGACGCACTCACCATCGTATGC	839	0.14862605047971317	No Hit
AGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	696	0.12329407763275371	No Hit
AATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCACTCA	621	0.11000807788784489	No Hit
CCTGTCTCTTATACACATCTGACGCACTCACCATCGTATGCCGTCT	606	0.10735087793886315	No Hit
ACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	597	0.10575655796947409	No Hit
AATGATACGGCGACTGTCTCTTATACACATCTGACGCACTCACCAT	588	0.10416223800008503	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.7714666326545074E-4	0.0	0.0	1.1066352054192707	0.0
2	1.7714666326545074E-4	0.0	0.0	1.3895384266541955	0.0
3	1.7714666326545074E-4	0.0	0.0	1.8807661238892903	0.0
4	1.7714666326545074E-4	0.0	0.0	4.168969573289117	0.0
5	1.7714666326545074E-4	0.0	0.0	4.427957994983206	0.0
6	1.7714666326545074E-4	0.0	0.0	5.717585703555688	0.0
7	1.7714666326545074E-4	0.0	0.0	6.725195924209571	0.0
8	1.7714666326545074E-4	0.0	0.0	7.198886101781387	0.0
9	1.7714666326545074E-4	0.0	0.0	8.914197242180746	0.0
10	1.7714666326545074E-4	0.0	0.0	10.122160338987856	0.0
11	1.7714666326545074E-4	0.0	0.0	12.366962855887646	0.0
12	1.7714666326545074E-4	0.0	0.0	13.094681348582117	0.0
13	1.7714666326545074E-4	0.0	0.0	13.39175630287828	0.0
14	1.7714666326545074E-4	0.0	0.0	13.750301149327552	0.0
15	1.7714666326545074E-4	0.0	0.0	14.029838583960432	0.0
16	1.7714666326545074E-4	0.0	0.0	14.534352280940436	0.0
17	3.542933265309015E-4	0.0	0.0	15.248076187236936	0.0
18	3.542933265309015E-4	0.0	0.0	16.189256409166276	0.0
19	3.542933265309015E-4	0.0	0.0	16.595099414707423	0.0
20	3.542933265309015E-4	0.0	0.0	16.976850474044472	0.0
21	3.542933265309015E-4	0.0	0.0	17.441860465116278	0.0
22	3.542933265309015E-4	0.0	0.0	17.930430962402394	0.0
23	3.542933265309015E-4	0.0	0.0	18.427327352861983	0.0
24	3.542933265309015E-4	0.0	0.0	18.75186003996429	0.0
25	3.542933265309015E-4	0.0	0.0	19.033523234556355	0.0
26	3.542933265309015E-4	0.0	0.0	19.310049175913722	0.0
27	3.542933265309015E-4	0.0	0.0	19.597203917067016	0.0
28	3.542933265309015E-4	0.0	0.0	19.899238977934612	0.0
29	3.542933265309015E-4	0.0	0.0	20.197376812210365	0.0
30	3.542933265309015E-4	0.0	0.0	20.625363150659695	0.0
31	3.542933265309015E-4	0.0	0.0	20.933066904751783	0.0
32	3.542933265309015E-4	0.0	0.0	21.211010019415273	0.0
33	3.542933265309015E-4	0.0	0.0	21.499227640548163	0.0
34	3.542933265309015E-4	0.0	0.0	21.799668381446367	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGCGAGC	55	5.456968E-12	40.000004	17
GAGCGCG	55	5.456968E-12	40.000004	20
CTAGAGA	20	0.0012552262	40.0	14
AACGTAG	20	0.0012552262	40.0	20
CTAGACG	40	1.9254003E-8	40.0	1
AACCGCG	20	0.0012552262	40.0	1
TCGTTGA	90	0.0	40.0	24
AGCCGAC	25	7.789607E-5	40.0	38
CGAGTTT	30	4.863108E-6	40.0	32
CTACTAC	20	0.0012552262	40.0	11
AATCACG	30	4.863108E-6	40.0	1
CGAAACG	20	0.0012552262	40.0	1
CAACGAC	25	7.789607E-5	40.0	10
CAACGAA	25	7.789607E-5	40.0	12
AACCCGA	30	4.863108E-6	40.0	24
TCCGACA	20	0.0012552262	40.0	23
GCGCGAC	20	0.0012552262	40.0	9
CGTGATT	20	0.0012552262	40.0	40
ACCGCAA	20	0.0012552262	40.0	28
CGCATCG	25	7.789607E-5	40.0	1
>>END_MODULE