FastQCFastQC Report
Sat 18 Jun 2016
SRR3548385_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3548385_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences734165
Sequences flagged as poor quality0
Sequence length46
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA208562.840778299156184No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA50610.6893545728821178No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA39040.5317605715336471No Hit
CTGTCTCTTATACACATCTGACGCTCGATTGCTCGTATGCCGTCTT33930.4621576893477624No Hit
AATGATACCTGTCTCTTATACACATCTGACGCTCGATTGCTCGTAT31500.4290588627896999No Hit
AATGATACGGCTGTCTCTTATACACATCTGACGCTCGATTGCTCGT25390.3458350643247771No Hit
AATCTGTCTCTTATACACATCTGACGCTCGATTGCTCGTATGCCGT25120.34215741692943685No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA20830.2837236860923634No Hit
AAACTGTCTCTTATACACATCTGACGCTCGATTGCTCGTATGCCGT11090.15105596153453243No Hit
ATGTTAGGGGGGGAGGGGAACTGGTATCTCCCAAGATTAACCTTCA9400.12803661302295807No Hit
TGCAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTT7970.10855870274393357No Hit
AAAAACTGTCTCTTATACACATCTGACGCTCGATTGCTCGTATGCC7360.10024994381372034No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAG304.8651073E-640.0000041
TTTGTCG304.8651073E-640.00000414
GACGTAA304.8651073E-640.00000422
CGCATGA304.8651073E-640.00000413
ATAGCCG304.8651073E-640.0000041
CACGCGA304.8651073E-640.00000430
GTGATCC304.8651073E-640.00000422
CGATTAG304.8651073E-640.0000041
CGCGACG304.8651073E-640.00000432
ACCTCGA304.8651073E-640.00000417
GGCTTAA304.8651073E-640.00000425
TGCGCAT304.8651073E-640.00000411
TACCTCG304.8651073E-640.0000041
CGTAAAT304.8651073E-640.00000424
TCGAGGT304.8651073E-640.00000419
ATTGCAA304.8651073E-640.00000432
TTACCGG600.040.0000042
AGTACGA304.8651073E-640.00000433
TCAACGA304.8651073E-640.00000419
GTACGAC304.8651073E-640.00000434